• Proceedings of the PSK Conference
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• 2002.10a
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• pp.417.1-417.1
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• 2002

The in vitro release of rhGH from PLGA microspheres was characterized. rhGH-loaded PLGA microspheres were prepared with 50:50 poly(D.L-lactide-co-glycolide) (PLGA) polymers using a double emulsion process. To simulate rhGH release under physiological conditions. the microspheres were suspended in a physiological buller at 37$^{\circ}C$. Quantification of the rhGH released and its molecular form analysis were carried out using SE-HPLC. (omitted)

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.154.2-154.2
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• 2001

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.156.1-156.1
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• 2001

• Journal of the Ergonomics Society of Korea
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• v.32 no.4
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• pp.397-403
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• 2013

Objective: This article aims to report the results of ergonomic evaluation of some university laboratories. Background: Laboratory researchers are at risk for musculoskeletal injuries during routine laboratory procedures. However, little attention has paid to the working conditions of laboratory researchers, especially in Korea. Method: A checklist and questionnaire was developed and used to analyze the work conditions of 27 laboratories of chemistry, biology and pharmacy. Results: It was found that glove box, clean bench, and computer workstation are better than microscopy, and fume hood tasks, from ergonomic point of view. Conclusion: No domestic guideline is available for ergonomic intervention of laboratories. Further attention and studies are anticipated to improve the working conditions of laboratories.

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.98-98
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• 2001

• Journal of Microbiology and Biotechnology
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• v.20 no.11
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• pp.1471-1480
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• 2010

An extremely thermostable xylanase gene, xynB, from the hyperthermophilic bacterium Thermotoga maritima MSB8 was successful expressed in Kluyveromyces lactis. The response surface methodology (RSM) was also applied to optimize the medium components for the production of XynB secreted by the recombinant K. lactis. The secretion level (102 mg/l) and enzyme activity (49 U/ml) of XynB in the optimized medium (yeast extract, lactose, and urea; YLU) were much higher than those (56 mg/l, 16 U/ml) in the original medium (yeast extract, lactose, and peptone; YLP). The secretory efficiency of mature XynB was also improved when using the YLU medium. When the mRNA levels of 13 characterized secretion-related genes in the K. lactis cultured in YLP and YLU were detected using a semiquantitative RT-PCR method, the unfolded protein response (UPR)-related genes, including ero1, hac1, and kar2, were found to be up-regulated in the K. lactis cultured in YLU. Therefore, the nutrient ingredients, especially the nitrogen source, were shown to have a significant influence on the XynB secretory efficiency of the host K. lactis.

• The Korean Journal of Nuclear Medicine
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• v.5 no.1
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• pp.11-17
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• 1971

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.20 no.4
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• pp.256-262
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• 2010

This study evaluated the airborne concentrations of fungi in university laboratories, hospital diagnostic laboratories in Seoul. The incubated fungi was identified by lactophenol cotton blue (LPCB) staining method. Variables such as types of ventilation, temperature and relative humidity were investigated to explain laboratory airborne fungal concentrations. A total of 97 air samples were collected from 10 facilities in two institutions. Aspergilus spp., including Aspergilus niger, Aspergillius flavos and Penicillium spp. were found as predominant species. Airborne fungal concentrations ranged from not detected (ND) to 1,890 CFU/$m^3$. Airborne fungal concentrations were high in general-ventilated facilities and in laboratories where relative humidity ( > 60 %) were high ( p < 0.001). Therefore, we suggest that relative humidity should be maintained to properly reduce the concentration of fungal in university and hospital laboratories.

• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.29-31
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• 2007

• Journal of the Korea Safety Management & Science
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• v.10 no.4
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• pp.83-91
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• 2008

In this study, actual status of safety management for the objects of 173 university, government research institution, and corporate affiliated research center was carried out through questionnaires. For unexpected accident status according to kinds, scale, and types of laboratories, frequency analysis using cross analysis and multiple replies analysis was used. The result of this study is judged to be used for laboratory safety education prevention programs, laboratory environment improvement, investment or improvement of laboratories through the actual safety management status of laboratories such as vulnerable works for generation of unexpected sudden accidents according to kind, scale, and type of laboratories, period of occurrence, prevention education, necessity for preliminary test, and cause of laboratory accidents.