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Effects of Bisphenol A on Vitellogenin Synthesis in the Hepatocyte Primary Culture in the Olive Flounder, Paralichthys olivaceus (넙치 Paralichthys olivaceus 초대 배양 간세포의 Vitellogenin합성에 미치는 Bisphenol A의 영향)

  • Yeo, In-Kyu;Choe, Mi-Kyung
    • Korean Journal of Ichthyology
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    • v.12 no.3
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    • pp.180-185
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    • 2000
  • The effects of bisphenol-A(BPA), a monomer of plastics used in many consumer products, on vitellogenin(VTG) synthesis were examined in primary hepatocyte culture of olive flounder, Paralichthys olivaceus. The hepatocytes were precultured for 2 days, and then estradiol-$17\beta(10^{-6}M)$ and BPA were simultaneously added to the incubation medium. The hepatocytes were cultured for 6 more days and then spent medium was analyzed by SDS-PAGE. BPA increased the rate of VTG to total protein concentrations in a concentration-dependent way, and a significant difference was obtained at concentrations of $10^{-6}M$ and $10^{-5}M$ (P<0.05). In particular, the rate of VTG to total protein concentrations was 26.36% at $10^{-5}M$ of BPA, and its level did not differ from the control level with $E_2$ alone. $E_2$ and/or BPA-primed VTG synthesis was markedly inhibited to about 80% of the control(with $E_2$) by the addition of tamoxifen($10^{-6}M$) to the incubation medium. Furthermore, In vivo $E_2$-primed VTG synthesis was significantly inhibited by in vitro $E_2$-free incubation of hepatocyte to about 22% of the control (with $E_2$) on Day 6. The effect of reducing was delayed in a BPA concentration-dependent way. These results suggest that BPA induce VTG synthesis by estrogenic activity through estrogen receptor mediated response and prolong VTG synthesis on vitellogenesis in olive flounder.

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An Effect of Academic Retention Concerned with High School Students' Cognitive Styles and Teaching Methods in 'The Weather and Climate' Unit ('날씨와 기후' 단원에서 고등학생들의 인지양식 및 수업방법에 따른 학업파지 효과)

  • Ryu, Sang-Ock
    • Journal of the Korean earth science society
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    • v.25 no.4
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    • pp.242-250
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    • 2004
  • The purpose of this study is to examine the interactive effects of teaching methods (expository and discovery) and learner's cognitive styles on the retention of learned content in Earth Science three weeks after an instruction is given. The subjects of this study were 1st graders of a boys' high school (n = 185) and co-educational high school (n = 189) in Gwangiu Metropolitan City of Korea. 92 students of the boys' high school and 95 students of the co-ed high school were assigned to the expository teaching group, and the rest of the boys' high school students and the co-ed high school students were to the discovery teaching group. The students were classified into field-independent learners, medium learners, and field-dependent learners based on their cognitive styles. The results of the study can be summarized as follows: First, the discovery teaching was more effective than expository teaching in terms of comprehension and application related to post-instruction retention of learned content. But there was no noticeable difference in knowledge domain. Second, the field-independent cognitive style was more effective than its counterpart, the field dependent cognitive style. Third, in the post-instruction retention test, not so much relevance was found between teaching methods and learner's cognitive style.

KOSPI 200 Derivatives and Volatility Asymmetry of Stock Markets (KOSPI 200 파생상품 거래와 주식수익률 변동성의 비대칭성)

  • Park, Jong-Won
    • The Korean Journal of Financial Management
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    • v.23 no.1
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    • pp.101-133
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    • 2006
  • We examine whether new derivatives on KOSPI 200 affect volatility asymmetry of KOSPI 200 portfolio, relative to the carefully matched non-KOSPI 200 portfolio. To test the effect or new derivatives trading, we use GJR-GARCH model and newly developed Volatility Ratio(down-market volatility to up-market volatility ratio). Our results show that KOSPI 200 portfolio experiences lower volatility asymmetry than non-KOSPI 200 portfolio after the trading of new derivatives on KOSPI 200, especially after the introduction of stock index options(KOSPI 200 options). For non-KOSPI portfolio, no significant reduction in volatility asymmetry occurred when trading of stock index options began. Also, we find that in the period of after January 1999, the period of after do-regulations and Financial Crisis in the Korean capital market, volatility asymmetry of stock markets was significantly decreased. This means that level of volatility asymmetry is closely related to the level of market regulations. Further, the results of the paper show that leverage effect and changes in foreign exchange ratio can be good candidates for explaining the stylized volatility asymmetry in the Korean stock market.

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Evaluation of Extruded Pellets and Raw Fish-Based Moist Pellet for Growth of Sub-Adult Flounder Paralichthys olivaceus (미성어기 넙치 사육을 위한 배합사료 및 습사료의 효과 비교)

  • Kim, Kyoung-Duck;Kang, Yong-Jin;Lee, Jong-Yun;Nam, Myong-Mo;Kim, Kang-Woong;Jang, Mi-Soon;Lee, Sang-Min
    • Journal of Aquaculture
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    • v.21 no.2
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    • pp.102-106
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    • 2008
  • This study was conducted to evaluate extruded pellets(EP) for the growth of sub-adult flounder Paralichthys olivaceus by comparing with raw fish-based moist pellet(MP). Two replicate groups of 150 fish per each tank(initial mean weight $594{\pm}6.5g$) were fed one of two EPs(EP1 and EP2) and a MP for 32 weeks. Survival was not significantly affected by experimental diets. Final mean weight of fish fed the EP1 was not significantly different from that of fish fed the MP, but that of fish fed EP2 was significantly lower than that of fish fed EP1 and MP (P<0.05). Feed efficiency and protein efficiency ratio of fish fed the EP1 and EP2 were significantly higher than those of fish fed MP(P<0.05). Daily feed intake of fish fed the EPs was significantly lower than that of fish fed MP(P<0.05). Condition factor was not significantly different among all groups. The contents of moisture, crude protein and lipid in dorsal muscle were significantly affected by experimental diets(P<0.05). Based on the results of this study, the dietary formulations used in EP1 and EP2 could be applied in the practical extruded pellet feeds for sub-adult flounder(594-1,126 g).

Temperature-dependent Fecundity and Life Table Parameters of Aphis gossypii Glover (Homoptera: Aphididae) on Cucumber Plants (오이에서 온도에 따른 목화진딧물 산자수 및 생명표)

  • Kim Ji-Soo;Kim Tae-Heung
    • Korean journal of applied entomology
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    • v.43 no.3 s.136
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    • pp.211-215
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    • 2004
  • Temperature-fecundity of the melon aphid, Aphis gossypii Glover, was studied at constant temperatures ranging from 15 to $32.5^{\circ}C$ under $60-70\%$ RH and a photoperiod of 16 : 8 (L : D) A life table parameters were constructed using the results. The longevity of A. gossypii gradually increased with decreasing temperature below $27.5^{\circ}C$. Also fecundity increased with decreasing temperature and the highest fecundity was 61.8 nymphs per female at $17.5^{\circ}C$. However. daily fecundity increased with increasing temperatures up to $22.5^{\circ}C$ showing 5.9 nymphs per day and thereafter decreased. Longevity and fecundity of the adult in the greenhouse with an average temperature of $21^{\circ}C$ and $65.6\%$ RH, were 20.0 days and 59.6, respectively, which were longer and higher than those in the growth chamber with similar conditions. net reproductive rate (Ro) was 54.9 at $17.5^{\circ}C$ while intrinsic rate of increase ($r_m$) and finite rate of increase ($\lambda$) were the highest 0.5 and 1.6 at $30^{\circ}C$, respectively. doubling time (DT) and mean generation time (T) were the shortest 1.4 and 6.8 at $30^{\circ}C$ indicating that optimal temperature for the development is $30^{\circ}C$.

Development Time and Development Model of the Green Peach Aphid, Myzus persicae (복숭아혹진딧물(Myzus persicae)의 발육과 발육모형)

  • Kim Ji-Soo;Kim Tae-Heung
    • Korean journal of applied entomology
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    • v.43 no.4 s.137
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    • pp.305-310
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    • 2004
  • The development of Myzus persicae (Sulzer) was studied at temperatures ranging from 15 to $32.5^{\circ}C$ under $70{\pm}5\%$ RH, and a photoperiod of 16:8 (L:D). Mortality of 1st-2nd nymph was higher than that of 3rd-4th nymph at the most temperature ranges whereas at high temperature of $32.5^{\circ}C$, more 3-4nymph stage individuals died. The total developmental time ranged from 12.4 days at $15^{\circ}C$ to 4.9 days at $27.5^{\circ}C$, suggesting that higher the temperature, faster the development. However, at higher end temperature ranges of 30 and $32.5^{\circ}C$, the development took 5.0 and 6.3 days, respectively. The lower developmental threshold temperature and effective accumulative temperatures for the total immature stage were $4.9^{\circ}C$ and 116.5 day-degrees. The nonlinear shape of temperature related development was well described by the modified Sharpe and DeMichele model. When the normalized cumulative frequency distributions of developmental times for each life stage were fitted to the three-parameter Weibull function, attendance of shortened developmental times was apparent with pre-nymph, post-nymph, and total nymph stages in descending order. The coefficient of determination $r^2$ ranged between 0.87 and 0.94.

Occurrence of Insect Pests in Rubus coreanus Miquel (복분자딸기에서 발생하는 해충 종류 및 발생 양상)

  • Lim, Ju-Rak;Choi, Seon-U;Kim, Ju-Hee;Lee, Ki-Kwon;Cheong, Seong-Soo;Ryu, Jeong;Hwang, Chang-Yeon
    • Korean journal of applied entomology
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    • v.49 no.2
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    • pp.97-103
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    • 2010
  • In this study, 5 species (Tetranychus kanzawai Kishida, Endoclyta excrescens (Butler), leaf roller caterpillar (Adoxophyes orana Fisher), Anomis mesogona (Walker), and Aulacaspis rosae (Bouche)) were found in Rubus coreanus Miquel in Jeonbuk province, from 2006 to 2008. T. kanzawai appeared in mid-May and showed three peaks in late June, mid-July, and late August. Its density was over 10 adults per leaf. A. mesogona appeared in mid-June and showed two peaks in late July, and early October. Of the 28 insect pests in pre-harvesting, the 7 main species were T. kanzawai, T. urticae, A. orana, Lygocoris lucorum Meyer, Frankliniella intonsa (Trybom), Psylliodes punctifrons Baly and Motschulskyia serrata (Matsumura). Of the 19 insect pests in after-harvesting, the 6 main species were T. kanzawai, E. excrescens, A. mesogona, A. orana, P. punctifrons and A. rosae. Growth of R. coreanus decreased when insect pests were not managed and yields decreased approx. 7% a year. Management of insect pests in field using pesticide was not different.

Seasonal Variation in Community Structure of Subtidal Seaweeds in Jeju Island, Korea (제주도 주변 해역 조하대 해조류 군집구조의 계절적 변동)

  • Kim, Bo Yeon;Ko, Jun-Cheol;Ko, Hyuck Joon;Park, Sung Eun;Cha, Hyung Kee;Choi, Han Gil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.46 no.5
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    • pp.607-618
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    • 2013
  • Marine macroalgal community structures and characteristics of ocean environmental factors were examined seasonally at four sites in Jeju Island, Korea, from March to November 2012. A total of 71 macroalgal species were identified, including 9 green, 7 brown, and 55 red algae. Peyssonnelia capensis occurred at all study sites and in all seasons. The average annual biomass of seaweed was 991.84 g wet $wt/m^2$, with seasonal variations from 543.80 g in autumn to 1,284.17 $g/m^2$ in summer. A green alga, Codium coactum, was the dominant species, occupying 21.31% (211.39 $g/m^2$) of the total algal biomass in Jeju Island. Subdominant species were Ecklonia cava and Lithophyllum okamurae, comprising 20.85% (206.75 $g/m^2$) and 19.64% (194.75 $g/m^2$), respectively, of the total algal biomass in Jeju Island. The vertical distribution of subtidal seaweeds was represented by L. okamurae at 5 m depth, C. coactum at 10 m depth, E. cava at 5-10 m depth and P. capensis at the 20 m depth level. In the present study, crustose coralline algae, which predominated on barren ground, were subdominant species at all study sites. Community indices varied between 0.51-0.63 for dominance index (DI), 5.53-8.14 for richness index (R), 0.51-0.63 for evenness index (J'), and 2.04-2.32 for diversity index (H'). On the basis of seaweed biomass and community indices, Sinchang was the best preserved coastal area, showing maximal values in biomass, and evenness- and diversity-indices, and minimal value in the dominance index, representing stable environmental conditions. In contrast, the Onpyung and Topyeong sites, located near tourist venues such as Udo and Seogwipo were relatively poor habitats based on community indices and biomass. The present results could imply that climate changes alter seaweed community structure, and long-term monitoring of the study sites is required.

A Study on Selection Attributes and Information Sources of Optical Shop (안경원 선택속성과 정보원천에 관한 연구)

  • Cha, Jung-Won
    • Journal of Korean Ophthalmic Optics Society
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    • v.21 no.3
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    • pp.173-179
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    • 2016
  • Purpose: This study is to help assist in the management of optical shops by using the importance sequence of optical shop selection attributes, which is related to the consumer's selection method of consumer, and by using the importance sequence of optical shop information sources which is related to a route for optical shop selection. Methods: Customer surveys were conducted from March 10 to March 31, 2015 targeting customers who have visited an optical shop in Seoul and Northern Gyeonggi-do regions. The analys method was descriptive statistics and data were analyzed by utilizing SPSS v.10.0 statistical package program. Results: The highest ranking five attributes among the importance of optical shop selection are "friendliness and politeness of staff", "cleanliness of an optical shop", "quick resolution of customer's complaints by staff", "eyes examination and glasses dispensing skill of staff", "customer's complaints and claims handling". The lowest ranking five attributes among the importance of optical shop selection are "provide free gifts", "scale or size of an optical shop", "opening time and closing time", "convenient parking facilities", "favorable countenance of staff". The two highestr ranking criteria among the importance of optical shop information sources are "previous utilization experience", "recommendation by a relative, a friend and a family etc". The two lowest ranking criteria among the importance of optical shop information sources are "advertisement" and "spatial exterior view of optical shop". Conclusions: It is shown that the important thing in management of an optical shop is an inner caliber like ability of ophthalmic optician, interaction with customers, and previous utilization experience rather than external factors like advertisement, exterior view, and bonus gift.

An analysis of the impact of Korean medicine health counseling on health perception change and satisfaction (한의건강상담이 건강인식 변화에 미치는 영향 및 만족도 분석)

  • Lee, Eunyoung;Jang, Eunsu;Kim, Sujung;Baek, Younghwa;Lee, Siwoo;Mun, Sujeong
    • Journal of Society of Preventive Korean Medicine
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    • v.21 no.1
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    • pp.19-30
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    • 2017
  • Objectives : The purpose of this study was to examine the changes of health perception, satisfaction survey and lifestyle in Korean medicine counseling for Daejeon University faculty members. Methods : In this study, the demographic questionnaire, lifestyle habit questionnaire, Mibyeong questionnaire, quality of life questionnaire(SF-12, EQ5D), pattern-based questionnaire, constitutional questionnaire(KS-15) and instrumental examination were performed on the first and second visit. We carried out 1:1 consultation at every visit. Results : A total of 189 participants participated in this study. 66% of respondents answered that they felt the change in health perception and more than 93% of the respondents felt satisfaction due to the Korean medicine health counseling. In the demographic characteristics, it was found that women were more satisfied with the Korean medicine health counseling than men(p <.05), the higher the education level(p <.05) and the higher the age(p <.05), managed their health continuously after receiving Korean medicine health counseling.(p <.05) In addition, in the change of lifestyle habit, the group who smoked and drank in the past answered that the consciousness to manage and improve health was higher than other group after Korean medicine health counseling.(p <.05) Conclusions : Korean medicine health counseling had a positive effect on overall health perception change and satisfaction was high. Since the Korean medicine health counseling has different effects on lifestyle and health perception change and the satisfaction of Korean medicine health counseling is different according to demographic characteristics and existing lifestyle habits. In the future, it will be expected that the effect of Korean medicine health counseling program on health care and disease prevention will be further enhanced if the Korean medicine health counseling program reflecting such characteristics is implemented.