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Circulating miR-195 as a Therapeutic Biomarker in Turkish Breast Cancer Patients

  • Cecener, Gulsah;Ak, Secil;Eskiler, Gamze Guney;Demirdogen, Elif;Erturk, Elif;Gokgoz, Sehsuvar;Polatkan, Volkan;Egeli, Unal;Tunca, Berrin;Tezcan, Gulcin;Topal, Ugur;Tolunay, Sahsine;Tasdelen, Ismet
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.9
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    • pp.4241-4246
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    • 2016
  • Background: Dysregulation of miRNA expression may be used as a biomarker for specific tumours because it may contribute to development of cancer. Circulating miRNA profiles have been highlighted for their potential as predictive markers in heterogeneous diseases such as breast cancer. In the literature, there is evidence that miR-195 levels are differentially expressed pre- and post-operative periods in breast cancer patients. At the same time, miRNA expression levels may vary because of ethnic origins. This study aimed to determine expression levels and potential roles of miR-195 in Turkish breast cancer patients. Materials and Methods: The expression patterns of miR-195 were initially examined in breast cancer tissues (luminal A and B type) (n=96). Subsequently, blood samples were prospectively collected from preoperative and postoperative Turkish breast cancer patients and disease free controls. Total RNA was isolated, and the expression level of miR-195 was quantified by real-time PCR. Results: We found that miR-195 level was altered in Turkish breast cancer patients, with down-regulation evident in breast cancer tissues compared to normal adjacent specimens. Furthermore, circulating levels of miR-195 was significantly decreased in post-operative blood samples compared with pre-operative levels (p=0.01 and <0.05). However, miR-195 was significantly increased in pre-operative blood samples of the luminal B type (p=0.04 and <0.05). Conclusions: This study represents the first report of a miR-195 expression profile in Turkish breast cancer patients. Our data suggests that miR-195 levels might be a clinically useful biomarker in the earliest stage of Turkish breast cancer patients.

Design of A CMOS Analog Multiplier using Gilbert Cell

  • Lee, Geun-Ho;Park, Hyun-Seung;Yu, Young-Gyu;Kim, Tae-Pyung;Kim, Jae-Young;Kim, Dong-Yong
    • The Journal of the Acoustical Society of Korea
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    • v.18 no.3E
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    • pp.44-48
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    • 1999
  • The CMOS four-quadrant analog multiplier for low-voltage low-power applications are presented in this thesis. The circuit approach is based on the characteristic of the LV (Low-Voltage) composite transistor which is one of the useful analog building block. SPICE simulations are carried out to examine the performances of the designed multiplier. Simulation results are obtained by 0.6㎛ CMOS parameters with 2V power supply. The basic configuration of the multiplier is the CMOS Gilbert cell with two LV composite transistors. The linear input range of the multiplier is over ±0.4V with a linearity error of less than 1.3%. The measured -3dB bandwidth is 288MHz and the power dissipation is 255 ㎼.

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GENETICAL STUDIES ON NATIVE CHICKENS IN INDONESIA

  • Yamamoto, Y.;Namikawa, T.;Okada, I.;Nishibori, M.;Mansjoer, S.S.;Martojo, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.4
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    • pp.405-410
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    • 1996
  • Phylogenetic analyses were carried out using four Indonesian native chicken breeds; Kampung, Bangkok, Pelung and Kedu. Gene frequencies of four blood group (A, B, D and E) and eight electrophoretic loci (akp, Akp-2, Es-1, Amy-1, Alb, Tf, Pas and Pa-1) were examined. Geographical and breed specific trends in the gene frequencies were not found in the local population of Kampung breed or in four native breeds. The values of average heterozygosity were estimated as 0.35-0.45. Genetic distances among the local populations of Kampung breed and other native breeds were comparatively small. In a cluster analysis, the Bangkok breed and Kampung E population showed distance from another cluster. The coefficient of gene differentiation for local populations of Kampung breed was estimated as 0.099.

Experimental and numerical investigation of composite conical shells' stability subjected to dynamic loading

  • Jalili, Sina;Zamani, Jamal;Shariyat, M.;Jalili, N.;Ajdari, M.A.B.;Jafari, M.
    • Structural Engineering and Mechanics
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    • v.49 no.5
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    • pp.555-568
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    • 2014
  • In this article, stability of composite conical shells subjected to dynamic external pressure is investigated by numerical and experimental methods. In experimental tests, cross-ply glass woven fabrics were selected for manufacturing of specimens. Hand-layup method was employed for fabricating the glass-epoxy composite shells. A test-setup that includes pressure vessel and data acquisition system was designed. Also, numerical analyses are performed. In these analyses, effect of actual geometrical imperfections of experimental specimens on the numerical results is investigated. For introducing the imperfections to the numerical models, linear eigen-value buckling analyses were employed. The buckling modes are multiplied by very small numbers that are derived from measurement of actual specimens. Finally, results are compared together while a good agreement between results of imperfect numerical analyses and experimental tests is observed.

Nonlinear QSAR Study of Xanthone and Curcuminoid Derivatives as α-Glucosidase Inhibitors

  • Saihi, Youcef;Kraim, Khairedine;Ferkous, Fouad;Djeghaba, Zeineddine;Azzouzi, Abdelkader;Benouis, Sabrina
    • Bulletin of the Korean Chemical Society
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    • v.34 no.6
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    • pp.1643-1650
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    • 2013
  • A non linear QSAR model was constructed on a series of 57 xanthone and curcuminoide derivatives as ${\alpha}$-glucosidase inhibitors by back-propagation neural network method. The neural network architecture was optimized to obtain a three-layer neural network, composed of five descriptors, nine hidden neurons and one output neuron. A good predictive determination coefficient was obtained (${R^2}_{Pset}$ = 86.7%), the statistical results being better than those obtained with the same data set using a multiple regression analysis (MLR). As in the MLR model, the descriptor MATS7v weighted by Van der Waals volume was found as the most important independent variable on the ${\alpha}$-glucosidase inhibitory.

Angioleiomyoma of the gingiva: a report of two cases

  • Arpag, Osman Fatih;Damlar, Ibrahim;Kilic, Soydan;Altan, Ahmet;Tas, Zeynel Abidin;Ozgur, Tumay
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.42 no.2
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    • pp.115-119
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    • 2016
  • Leiomyoma is a type of benign smooth muscle neoplasm that is a common neoplasm of the uterus and gastrointestinal tract but rarely affects the head and neck region and is especially unlikely to affect the oral cavity. The diagnosis of leiomyoma is mainly determined by histopathological studies due to variation in its clinical appearance and symptoms. In the present paper we report two rare cases of gingival angioleiomyoma in the posterior maxilla and mandible. After total excision, hematoxylin-eosin and smooth muscle actin staining confirmed the diagnosis of angioleimyoma.

Effect of nitrogen sources on production of exopolymer by Pseudomonas elodea ATCC 31461

  • Lee, Nam-Gyu;Kim, Hyeon-Suk;Seo, Hyeong-Pil;Jeong, Dae-Yeong;Kim, Ji-Mo;Lee, Jin-U
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.247-250
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    • 2000
  • Extracellular polysaccharide was produced by Pseudomonas elodea ATCC 31461 under aerobic condition. Nitrogen sources in medium affected cell growth and production of exopolymer. Ammonium nitrate limitation was found to be essential for higher production of exopolymer. Conversion rate of exopolymer from glucose under ammonium nitrate limitation was about 5 times higher than with ammonium nitrate.

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