• Journal of Radiopharmaceuticals and Molecular Probes
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• v.6 no.2
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• pp.92-101
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• 2020

The cyclic Arg-Gly-Asp (cRGD) peptide is well-known as a binding molecule to the integrin α_vβ₃ receptor which is highly expressed on activated endothelial cells and new blood vessels in tumors. Although numerous results have been reported by the usage of cRGD peptide-based ligands for cancer diagnosis and therapy, the distinct mechanisms, and functions of cRGD-integrin binding to cancer cells are still being investigated. In this study, we evaluated the internalization efficacy of different types of cRGD peptides (monomer, dimer and tetramer form) in integrin α_vβ₃ overexpressing cancer cells. Western blot and flow cytometric analysis showed U87MG expresses highly integrin α_vβ₃, whereas CT-26 does not show integrin α_vβ₃ expression. Cytotoxicity assay indicated that all cRGD peptides (0-200 µM) had at least 70-80% of viability in U87MG cells. Fluorescence images showed cRGD dimer peptides have the highest cellular internalization compare to cRGD monomer and cRGD tetramer peptides. Additionally, transmission electron microscope results clearly visualized the endocytic internalization of integrin α_vβ₃ receptors and correlated with confocal microscopic results. These results support the rationale for the use of cRGD dimer peptides for imaging, diagnosis, or therapy of integrin α_vβ₃-rich glioblastoma.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.6
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• pp.591-598
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• 2017

Propofol is known to cause vasorelaxation of several systemic vascular beds. However, its effect on the pulmonary vasculature remains controversial. In the present study, we investigated the effects of propofol on human pulmonary arteries obtained from patients who had undergone surgery. Arterial rings were mounted in a Multi-Myograph system for measurement of isometric forces. U46619 was used to induce sustained contraction of the intrapulmonary arteries, and propofol was then applied (in increments from $10-300{\mu}m$). Arteries denuded of endothelium, preincubated or not with indomethacin, were used to investigate the effects of propofol on isolated arteries. Propofol exhibited a bifunctional effect on isolated human pulmonary arteries contracted by U46619, evoking constriction at low concentrations ($10-100{\mu}m$) followed by secondary relaxation (at $100-300{\mu}m$). The extent of constriction induced by propofol was higher in an endothelium-denuded group than in an endothelium-intact group. Preincubation with indomethacin abolished constriction and potentiated relaxation. The maximal relaxation was greater in the endothelium-intact than the endothelium-denuded group. Propofol also suppressed $CaCl_2$-induced constriction in the 60 mM $K^+$-containing $Ca^{2+}$-free solution in a dose-dependent manner. Fluorescent imaging of $Ca^{2+}$ using fluo-4 showed that a 10 min incubation with propofol ($10-300{\mu}m$) inhibited the $Ca^{2+}$ influx into human pulmonary arterial smooth muscle cells induced by a 60 mM $K^+$-containing $Ca^{2+}$-free solution. In conclusion, propofol-induced arterial constriction appears to involve prostaglandin production by cyclooxygenase in pulmonary artery smooth muscle cells and the relaxation depends in part on endothelial function, principally on the inhibition of calcium influx through L-type voltage-operated calcium channels.

• Investigative Magnetic Resonance Imaging
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• v.25 no.3
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• pp.172-182
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• 2021

Purpose: We evaluated the diagnostic performance of LI-RADS version 2018 using gadoxetic acid enhanced MRI for recurrent but untreated HCC in patients with prior history of HCC. Materials and Methods: We enrolled 50 consecutive patients who 1) prior history of treatment of HCC, 2) underwent liver surgery for radiological/clinical diagnosis of new HCC between 2013 to 2018, 3) had gadoxetic acid enhanced MRI within one month before surgery, and 4) did not have more than five HCCs or infiltrative tumors only. Two radiologists reviewed MRI and determined the presence of LR3, LR4 and LR5 observations except previously treated tumors based on LI-RADS version 2018 in consensus. We sub-classified LR4 into LR4m (LR4 with major features only) and LR4u (LR4 upgraded from LR3 by ancillary features). LR4u were further sub-classified into LR4ua (with arterial phase hyperenhancement) and LR4un (without arterial phase hyperenhancement). Results: PPV for LR5, LR4 and LR3 observations for recurrent HCC were 100%, 61.5% and 25.0%, respectively. 100% (3/3) of LR4m were HCC. However, PPV of LR4u was 56.5%. PPV of LR4ua and LR4un were 73.3% and 25.0%, respectively. Sensitivity of LR5 and LR5+LR4 observations as a diagnostic threshold were 32.1% and 89.3%, respectively. Sensitivity for LR5+LR4m+LR4ua observations for diagnosis of HCC were 83.7% and significantly superior to that of LR5 without significant deterioration of specificity (75.0%). Conclusion: In patients with prior history of HCC, LR4 observations by major features or with APHE may be regarded as recurrent HCCs given high sensitivity and comparable specificity/PPV to LR5 observations.

• Journal of Internet Computing and Services
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• v.9 no.3
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• pp.25-41
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• 2008

In this paper, we describes a Compact Distributed Object Group Framework(CDOGF) for mobile collaboration service in ubiquitous computing. CDOGF support network connections of sensors and devices, management of mobile computing devices, and grouping of objects for mobile collaboration service according to the logical area. For this, we defined methods for detail functions and interface. And, we classified into four interactions of that sensors and a application, the CDOGF and a application, between the components of the CDOGF, the CDOGF on a mobile device and the DOGF on a server. We also defined mobile collaboration environment by analyzing existing technologies. In this environment we used the TMO scheme for interaction and the TMOSM for distributed middleware. Finally, we implemented u-hospital application that applied this environment. This application collects environment and health information by PDAs in wards. Also, It provides the information service for patient management task.

• Journal of the Korea Society of Computer and Information
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• v.16 no.1
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• pp.175-182
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• 2011

This study proposes CAOPI(Computer Aided Organ Prediction Index) system based on APACHE II(Acute Physiology And Chronic Health Evaluation) for classifying disease severity and predicting the conditions of patients' major organs. The existing ICU disease severity evaluation is mostly about calculating risk scores using patients' data at certain points, which has limitations on making precise treatments. CAOPI system is designed to provide personalized treatments by classifying accurate severity degrees of emergency patients, predicting patients' mortality rate and scoring the conditions of certain organs.

• Clinical and Experimental Pediatrics
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• v.59 no.10
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• pp.402-407
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• 2016

Purpose: Buckwheat allergy is one of the most severe types of food allergy in some countries, especially among children. However, few studies have investigated this condition. The aim of this study was to report the clinical and laboratory findings in Korean children with buckwheat allergy. Methods: Thirty-seven subjects, aged 1 to 14 years, were enrolled by retrospective medical record review from January 2000 through May 2015 at the Department of Pediatrics in Ajou University Hospital. The demographic profile, previous exposure to buckwheat pillows, clinical symptoms, and laboratory findings of each subject were recorded. Results: Twenty-six of the 37 children had immediate-type allergic symptoms to buckwheat, while 11 subjects were tolerant to buckwheat. Seventeen out of 26 buckwheat allergic children (65.4%) had anaphylaxis. The median buckwheat specific IgE level in the buckwheat allergic group ($7.71kU_A/L$) was significantly higher (P<0.001) than in the buckwheat tolerant group ($0.08kU_A/L$) with an optimal cutoff value of $1.27kU_A/L$ (sensitivity 84.6%, specificity 100%). When adjusted for age, the difference between the 2 groups showed no statistical significance (P=0.063). In subjects who had anaphylaxis, buckwheat-specific IgE levels ranged from 0.37 to $100kU_A/L$. Conclusion: Almost two-thirds of buckwheat-allergic children had anaphylaxis, and a wide-range of buckwheat specific IgE levels were observed in these children. Anaphylaxis occurred in a subject with a remarkably low IgE level ($0.37kU_A/L$).

• The Journal of the Korea Contents Association
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• v.14 no.5
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• pp.1-7
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• 2014

Nowadays hospitals have been improving their job performances through informatization and also establishing an advanced, integrated medical information system through their manager's decision making support system in order to play roles as a hub hospital providing high quality medical services integrated with ICT technology. This study connects the OCS system and HIS system to the integrated medical information system to design an optimized, customized mobile health care and medical treatment environment and also investigates the systematic medical system that can perform patients' cure and medical treatment promptly and accurately in order to maximize convenience of treatment by inquiring into patients' information and information of medical treatment promptly.

• Journal of Preventive Medicine and Public Health
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• v.7 no.2
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• pp.359-366
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• 1974

The authors collected epidemiological and clinical informations on 1,307 CO patients from the questionairefilled by attendants of patients and from the clinical records kept in the hospital. All patients were treated by hyperbaric oxygenation at the Hyperbaric Chamber Unit of Seoul National University Hospital during 5 years, from January, 1969 to December, 1973. The following findings were obtained. 1. Female showed higher incidence rate than male. The highest incidence rate was observed in the age group of 15-29 years in both sex. 2. The most important variable influencing the recovery of the patients was the starting time of treatment. It was evident that earlier the starting time, shorter the recovery time. 3. Duration of admission was within 5 days in 57.1 percent of the admitted cases and 76.1 percent of them was discharged within 8 days. 4. As the complications, pulmonary edema, bronchopneumonia and trophic changes were observed. Also the neurological disorders were found. 5. The fatality rate followed by the hyperbaric oxygenation was 1.07 percent.

• Journal of Sasang Constitutional Medicine
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• v.14 no.3
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• pp.146-152
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• 2002

Purpose Because Taeyangin constitutions are very rare, There are few references about Taeyangin diseases. I suppose to gather basic materials on the case of a diagnosis and treatment on Taeyangin constitution patient. So I want to accomplish the theory of Taeyangin diseases. Methods I studied I patients with sequelae of CVA who were diagnosed by Taeyangin at department of Sasang constitutional Medicine in Kangnam Kyung Hee Oriental Hospital, and then I investigated the Ordinary symptoms as the diagnosis and treatment on a Taeyangin constitution Conclusion & Results To diagnose Taeyangin constitution, It is important to find out one's ordinary symptomes(especially appearance, personality, taste of food, existence of $y{\breve{u}}lk{\breve{u}}k{\cdot}haey{\breve{u}}k$, condition of urine and feces)

• Journal of Digital Contents Society
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• v.9 no.1
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• pp.27-31
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• 2008

u-Health is a representative realization method of ubiquitous IT and it is being embossed as an industry that can make our lives abundant. Through the u-Health, the diagnosis will go beyond the restriction of space, which is based on hospital, and be positioned as a universal value in a daily life by combining diagnosis and life naturally. The purpose of this study is to suggest systematic, intelligent, mobile medical information system that has the same effect as the assistant of specialist by providing scientific and objective knowledge, which is suitable for u-Health age. Mobile medical information system can provide user with the opinion of specialist by planting the experience, knowledge and decision making process of specialist that are necessary for solving the problem requiring special medical knowledge and passing through an inference process.