• The Journal of the Korean Society for Microbiology
• /
• v.21 no.1
• /
• pp.33-45
• /
• 1986

On hundred and forty stains of shigella cultures isolated from the twelve hygiene laboratories of cities and provincial general hospital laboratories in 1983 were tested for their resistance to thirteen antimicrobial drugs and their R-plasmid transfer. Antimicrobial drugs were used amikacin, ampicillin, cephalothin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, rifampicin, streptamycin, tetracycline, tobramycin, cefoperazone and piperacillin. All strains were resistant to one or more of thirteen antimicrobial drugs but 94.3% were susceptible to amikacin, gentamicin and tobramycin of total isolated. The most strains commonly found resistance was to chloramphenicol (94%) followed by streptamycin (93%), tetracyline (92%) piperacillin (90%) ampicillin (83%), cefoperazone (42%), nalidixic acid (14%), cephalothin (17%), rifampicin (22%) and kanamycin (6%), sixty percent of strains among 140 were resistance to ampicillin, chloramphenicol, streptomycin, tetracycline at the same time. The transfer of drug resistance by conjugation was tested and ninety four strains (94.3%) were resistant to one or more drugs were found to transfer their drug resistance of E. coli. percentage of transfer frequency by conjugation was one strains (54%), the transfer frequency of drug resistance varied by donor strains and recipients, but not by selecting drugs. Resistance to nalidixic acid was not transferred by conjugation to recipients. Percentage of plasmid curing after the treatment of acriflavine, acridine orange was about 8%. Among strains cured two strains were tested compare original strains with them in biochemical properties in arginine dihydrolase and arabinose fermentation reaction. It was found to growth curves of No.2 shigella flexneri, serotype 1b, and its derivatives cured with acriflavine in $M{\ddot{u}}ller$ Hinton broth medium (pH 7.4, $38^{\circ}C$) by temperature Gradient Biophoto Recorder TN-1120 (Tokyo, Japan).

• Korean Journal of Clinical Laboratory Science
• /
• v.38 no.1
• /
• pp.54-58
• /
• 2006

To find out the effectiveness of clinical examination for the diagnosis of respiratory tuberculosis, a 78 respiratory tuberculosis patient,s group was matched by sex and age with 78 control healthy subjects. In the result of blood chemistry, mean values of $123.5{\pm}62.04mg/dL$ in glucose, $429.01{\pm}150.77IU/L$ in LDH, and $44.51{\pm}43.76IU/L$ in ${\gamma}$-GTP, were higher than that of the controls (healthy subjects), and $3.51{\pm}0.68mg/dL$ in albumin was lower than that of the controls. In the result of the haematology examination, mean values of $12.52{\pm}3.27g/dL$ in hemoglobin, $36.72{\pm}7.28%$ in hematocrit, and $24.61{\pm}12.36%$ in lymphocyte, were lower than that of the controls, $9.23{\pm}5.25%$ in monocyte $78.30{\pm}37.35mm/hr$ in ESR, and $48.45{\pm}35.15U/L$ in ADA were higher than that of the controls. For the comparisons of the tuberculosis patients values from normal reference values, 22.2% in glucose, 22.4% in LDH, 25.0% in ${\gamma}$-GTP, 35.4% in albumin, 88% in ESR, and 88.6% in ADA, showed abnormal values. We concluded that the values of glucose, ${\gamma}$-GTP, albumin, WBC, RBC, hemoglobin, hematocrit, lymphocyte, monocyte, ADA, and the ESR were useful in tuberculosis diagnosis.

• Mass Spectrometry Letters
• /
• v.3 no.4
• /
• pp.93-100
• /
• 2012

Cellular processes such as proliferation, differentiation, and adaptation to environmental changes are regulated by protein phosphorylation. In order to enhance the understanding of molecular dynamics for biological process in detail, it is necessary to develop sensitive and comprehensive analytical methods for the determination of protein phosphorylation. Neural stem cells hold great promise for neural repair following an injury or disease. In this study, we made differentiated oligodendrocytes from human neural stem cells using over-expression of olig2 gene. We confirmed using quantitative phosphoproteome analysis approach that combines stable isotope labeling by amino acids in cell culture (SILAC) and $TiO_2$ micro-column for phosphopeptide enrichment with $MS^2$ and $MS^3$ mass spectrometry. We detected 275 phosphopeptides which were modulated at least 2-fold between human neural stem cells and oligodendrocytes. Among them, 23 phosphoproteins were up-regulated in oligodendrocytes and 79 phosphoproteins were up-regulated in F3 cells.

• Journal of The Korean Society of Integrative Medicine
• /
• v.4 no.2
• /
• pp.1-11
• /
• 2016

Purpose : The purpose of this study was to identify whether virtual reality-based exercise could improve on balance, gait and fall efficacy in patients with Parkinson's disease. Methods : Ten patients with Parkinson's disease were randomly divided into either an experimental or control group. The experimental subjects performed vertual reality-based exercise, whereas the control subjects performed conventional physical therapy for 4 weeks. The balance, gait and fall efficacy of all subjects were assessed by using the Measurement Training and Documentation (MTD) balance system, force platform system, Korean version of Berg Balance scale (K-BBS), 6 Minute Walk Test (6MWT), and Korean version of Fall efficacy scale (K-FES) at pre training and post training. Wilcoxon signed rank test was used to analyze change before and after intervention in intra-group. Mann Whitney U test was used to analyze changes of all variables in inter-groups. Results : Subjects in the experimental group showed significant improvements in difference of weight distribution, K-BBS scores, antero-posterior and medio-lateral sway length, ground reaction force (GRF), 6MWT, and step length following training. The changes of difference of weight distribution, K-BBS scores, AP Sway Length, GRF, 6MWT, step length and K-FES scores in the experimental group were significantly more than them of the control group. Conclusion : The result of this study suggest that virtual reality-based exercise training is an intervention to improve on balance, gait, and falls efficacy in patients with Parkinson's disease.

• Journal of Korean Medicine Rehabilitation
• /
• v.21 no.2
• /
• pp.15-30
• /
• 2011

Objectives : The present study investigated the effects of Achyranthis Radix on short-term memory, apoptotic neuronal cell death in the hippocampus following transient global ischemia in gerbils. Methods : The gerbils were divided into 5 groups(n=10); Sham operation group, ischemia-induced group, ischemia-induced and 50 mg/kg Achyranthis Radix-treated group, ischemia-induced and 100 mg/kg Achyranthis Radix-treated group, ischemia-induced and 200 mg/kg Achyranthis Radix-treated group. For this study, a step-down avoidance task, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay, immunohistochemistry for caspase-3 and BrdU(5-Bromo-2'-deoxyuridine), and western blotting for bax, bcl-2 were performed. Results : The results revealed that ischemic injury impaired short-term memory and increased apoototic neuronal cell death in the hippocampal CA1(cornu ammonis area 1) region. Ischemic injury enhanced cell proliferation in the hippocampal CA1 region, the compensatory and adaptive process for excessive apoptosis. Achyranthis Radix treatment improved short-term memory by suppressing ischemia-induced apoptotic neuronal cell death in the hippocampal CA1 region. Also, Achyranthis Radix suppressed the ischemia-induced increase in cell proliferation in the hippocampal CA1 region. Conclusions : We showed that Achyranthis Radix alleviates ischemia-induced apoptotic neuronal cell death, thus facilitates the recovery of short-term memory impairment induced by ischemic cerebral injury.

• Journal of Periodontal and Implant Science
• /
• v.38 no.sup2
• /
• pp.309-316
• /
• 2008

Purpose: The aim of this study was to determine the survival rates of implants placed in grafted maxillary sinuses and compare the results obtained with graft materials, implant surfaces and timing of implant placement. Materials and Methods: Between January 1996 and December 2005, 391 implants were placed in 161 patients who underwent sinus grafting treatment simultaneously or separately at Ewha Womans University Hospital. According to inclusion criteria, 272 implants were placed in 102 patients with 112 sinus grafts (30 females, 72 males), aged 26 to 88 years (mean age $49.0{\pm}9.7$). The follow-up period ranged from 12 to 134 months (mean F/U $47{\pm}32$). Survival rates were evaluated according to graft material, implant surface and timing of implant placement. The Kaplan-Meier procedure and the log rank (Mantel-Cox) test were used to estimate survival rates and test for equality of survival rates between different groups of patients. Results: Ten-year cumulative survival rate for implants placed in the grafted sinuses was 90.1%. The survival rates for autogenous bone, combination and bone substitutes were 94.6%, 85.9% and 100%, respectively (p > 0.05). According to implant surface, survival rates were 84.8% in machined group and 97.5% in rough group (p < 0.05). The survival rates were 92.9% in delayed group and 86.0% in simultaneous group (p > 0.05). Conclusion: Ten-year cumulative survival rate for implants placed in the grafted sinuses was 90.1%. Rough-surfaced implants have a higher survival rate than machined-surfaced implants when placed in grafted sinuses (p < 0.05).

• Journal of Sasang Constitutional Medicine
• /
• v.7 no.2
• /
• pp.89-100
• /
• 1995

This study was conducted for the purpose of finding out an objective classification method for Sasang Constitutional medicine, which divides people into 4 groups of constitution and presents comprehensively physiology, pathology, diagnosis, therapy and recuperation regarding each constitution. Questionnaire of Sasang Constitution Classification(I) was administered to 328 inpatients at Kyung Hee Oriental Medicine Hospital. Data was collected during 10 months from June 1994 to Mar. 11, 1995. For the purposes of this study, the collected data was analyzed by crosstabs, variation analysis, and discrimination analysis. The analyzing program was SPSS PC+V4.0. For the purposes of this study, the collected data was analyzed by crosstabs, cariation analysis, and discrimination analysis. The analyzing program was SPSS PC+V4.0. The results were as follows : 1. There was significant differences of each group scales through variation analysis. The questions of each group had Sasang constitutional diagnostic discrimination abilities 2. The diagnostic discrimination abilities(Hit-ratio=56.10%) of the Questionnaire for Sasang Constitution Classification(I) were found to have over 20% improvement than the propotional chance criteria(33.3%), Especially, Hit-ratio for $So{\breve{u}}m$-In(63%) was higher than that of SoYang-In(55%) and $Ta{\breve{e}}um$-In(56.3%). 3. Through discrimination analysis on good questions of each group, the diagnostic discrimination abilities of the Questionnaire for Sasang Constitution Classification(I) was 57.93%. 4. This would be on the ground that the Questionnaire for Sasang Constitution Classification(I) could be used as a tool for Sasang constitution classification.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.19 no.1
• /
• pp.236-250
• /
• 2006

Purpose : A study on the recognition about osteoporosis and TKM treatment for osteoporosis of women who visited Sangji Oriental Medical Hospital. Methods : We studied the recognition and realities of TKM treatment for osteoporosis with questionnaire from 14th september, 2005 to 14th October, 2005 in Sang-ji Oriental medical clinic. A questionnaire was given to 184 women and 171 women answered. Results : 1) In 171 women, 169(98.8%) women have heard about osteoporosis, 1(0.6%) woman hasn't heard about it, and 1(0.6%) woman doesn't know. 2) In 171 women, 85(49.7%) women checked up for osteoporosis. 31(36.1%) women of them were diagonosed as osteoporosis, 50(58.1%) women of them were not diagonosed as osteoporosis, and 5(5.8%) women of them didn't know.3) In 31 women, 18 women is taking none medical care, 5 women calcium supplement, 4 women HRT, and 4 women both therapy. 4) In 171 women, 74(43.3%) women know about TKM treatment for osteoporosis, 96(56.1%) women don't know. 5) In 171 women, 140(81.9%) women have intention of TKM treatment for osteoporosis, 17(9.9%) women don't have, and 14(8.2%) women don't know. Conclusion : The guide that treats osteoporosis with T]U is needed on purpose to make intention of TKM treatment for osteoporosis to real demand. If the guide be informed to oriental doctors, It can expand the range of oriental gynecology.

• Journal of the Korea Society of Computer and Information
• /
• v.17 no.9
• /
• pp.113-125
• /
• 2012

We developed a Smartphone application based on PHR(Personal Health Records) provided by a tertiary hospital to provide users personalized diet, exercise contents. It uses Relevance ratio algorithm of diet and excercise being correlated with diseases listed on PHR to provide personalized contents to management of individual diseases and record the amounts of food taken and exercise along with calories consumed through exercise. Besides, developed U-Health Service also considering users' conditions and conveniences, as a service to use contents and record suitable for the individuals who share the information on restaurants locations, their menus, and nutrition based on users' location information.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.11
• /
• pp.1516-1524
• /
• 2014

Flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) can utilize a variety of external electron acceptors and also has stricter substrate specificity than any other glucose oxidoreductases, which makes it the ideal diagnostic enzyme in the field of glucose biosensors. A gene coding for a hypothetical protein, similar to glucose oxidase and derived from Aspergillus terreus NIH2624, was overexpressed in Pichia pastoris GS115 under the control of an AOX1 promoter with a level of 260,000 U/l in the culture supernatant after fed-batch cultivation for 84 h. After a three-step purification protocol that included isopropanol precipitation, affinity chromatography, and a second isopropanol precipitation, recombinant FAD-GDH was purified with a recovery of 65%. This is the first time that isopropanol precipitation has been used to concentrate a fermentation supernatant and exchange buffers after affinity chromatography purification. The purified FAD-GDH exhibited a broad and diffuse band between 83 and 150 kDa. The recombinant FAD-GDH was stable across a wide pH range (3.5 to 9.0) with maximum activity at pH 7.5 and $55^{\circ}C$. In addition, it displayed very high thermal stability, with a half-life of 82 min at $60^{\circ}C$. These characteristics indicate that FAD-GDH will be useful in the field of glucose biosensors.