• 수도
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• v.24 no.4 s.85
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• pp.97-103
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• 1997

최 정진씨는 현재 미국 필라델피아시의 수도국장으로 재직하고 있으며, 본 원고는 수자원공사의 초청으로 방한하여 지난 5월 세미나에서 발표한 내용을 간추린 것임. 미국의 안전음용수 법의 개요와 규제 추세를 밝힌 것으로 우리나라의 경우에도 많은 참고가 될 것으로 생각된다.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.1
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• pp.59-64
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• 2012

Hypoxic pulmonary vasoconstriction (HPV) is physiologically important response for preventing mismatching between ventilation and perfusion in lungs. The HPV of isolated pulmonary arteries (HPV-PA) usually require a partial pretone by thromboxane agonist (U46619). Because the HPV of ventilated/perfused lungs (HPV-lung) can be triggered without pretone conditioning, we suspected that a putative tissue factor might be responsible for the pretone of HPV. Here we investigated whether HPV can be also observed in precision-cut lung slices (PCLS) from rats. The HPV in PCLS also required partial contraction by U46619. In addition, $K^+$ channel blockers (4AP and TEA) required U46619-pretone to induce significant contraction of PA in PCLS. In contrast, the airways in PCLS showed reversible contraction in response to the $K^+$ channel blockers without pretone conditioning. Also, the airways showed no hypoxic constriction but a relaxation under the partial pretone by U46619. The airways in PCLS showed reliable, concentration-dependent contraction by metacholine ($EC_{50}$, ~210 nM). In summary, the HPV in PCLS is more similar to isolated PA than V/P lungs. The metacholineinduced constriction of bronchioles suggested that the PLCS might be also useful for studying airway physiology in situ.

• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.97-104
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• 2008

Plasminogen activators (PAs) are serine protease that cleave plasminogen to form the active protease plasmin. PA/plasmin system playa role in mammalian fertilization and motility and acrosome reaction of sperm. The present study was undertaken to identify PAs in porcine gametes and investigate a possible role of plasminogen in in vitro fertilization in the pig. When boar spermatozoa were preincubated in a fertilization medium (mTBM) for 0, 2, 4 or 6 h, the activity of tPA-PAI ($110{\sim}117\;kDa$), tPA ($62{\sim}70\;kDa$), and uPA ($34{\sim}38\;kDa$) was observed in the sperm incubation medium and sperm sample. PA activities in the sperm incubation medium significantly (p<0.05) increased according to increasing incubation times, while PA activities in sperm significantly (p<0.05) decreased at the same times. In addition, the rate of acrosome reaction in spermatozoa increased by increasing culture times. When oocytes were separated from porcine cumulus-oocytes complexes at 0, 22 or 44 h of maturation culture, no PA activities were observed in cumulus free-oocyte just after aspiration from follicles. However, the activity of tPA-PAI ($108{\sim}113\;kDa$) and tPA ($75{\sim}83\;kDa$) was observed at 22 h of in vitro culture and significantly (p<0.05) increased as the duration of the culture increased. On the other hand, when porcine oocytes were activated by sperm penetration or calcium ionophore, plasminogen significantly (p<0.05) increased ZP dissolution time (sec) in activated oocytes by sperm penetration. These results suggest that supplementation of plasminogen to fertilization medium may playa positive role in the improvement of in vitro fertilization ability in the pig.

• Journal of Embryo Transfer
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• v.32 no.4
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• pp.267-274
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• 2017

Alpha lipoic acid (ALA) is a biological membranes compound. As the antioxidant, it decreases the oxidized forms of other antioxidant substances such as vitamin C, vitamin E, and glutathione (GSH). To examine the effect of ALA on the in vitro maturation (IVM) of porcine oocytes, we investigated intracellular GSH and reactive oxygen species (ROS) levels, and subsequent embryonic development after parthenogenetic activation (PA). Intracellular GSH levels in oocytes treated with 50uM ALA increased significantly (P < 0.05) and exhibited a significant (P < 0.05) decrease in intracellular ROS levels compared with the control group. Oocytes matured with 50 uM of ALA during IVM displayed significantly higher cleavage rates (67.8% vs. 83.4%, respectively), and higher blastocyst formation rates and total cell number of blastocysts after PA (31.6%, 58.49 vs. 46.8%, 68.58, respectively) than the control group. In conclusion, these results suggest that treatment with ALA during IVM improves the cytoplasmic maturation of porcine oocytes by increasing the intracellular GSH levels, thereby decreasing the intracellular ROS levels and subsequent embryonic developmental potential of PA.

• The Journal of Korean Medicine
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• v.26 no.3 s.63
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• pp.215-227
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• 2005

Objectives : This study was carried out to investigate the effects of Pyungjintang on indomethacin-induced gastric mucosal lesions of mire. Methods : Experimental mice were classified into not-treated group (NOR group), gastro-inflammation elicitated group (CON group), misoprostol-administered group after gastro-inflammation elicitation (MA group), and Pyungjintang-administered group after gastro-inflammation elicitation (PA group). This study examined the morphological change, distribution of mast cells, mucus surface cells, neutral mucus secreting cells, acid mucus secreting cells, PNA reaction, angiogenesis (MIP-2), COX-1, Hsp70, NF-kB p50, COX-2IL-12B, ICAM-1, BrdU and apoptotic cells of gastric mucosa. Results : 1. The scars of diapedesis, dilatation of right gastric artery and the hemorrhagic erosions of gastric mucosa were reduced in the MA and PA groups. 2. Gastric perforation was observed in the gastro-inflammation elicitated group, but not in the MA and PA groups. 3. The COX-1 positive cellsl, cell proliferation of gastric mucosa, neutral mucus secreting ce31s, acid mucus secreting cells and PNA positive reaction of surface mucus cells were increased in the MA and PA groups. 4. The distribution of apoptotic cells, mast cells, MIP-2, Hsp70, NF-kB p50, COX-2, IL-l2B and ICAM-1 were decreased in the MA and PA groups. Conclusions : Pyungjintang had excellent effects on indomethacin-induced gastric mucosal lesions in mice.

• Korean Journal of Pharmacognosy
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• v.29 no.3
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• pp.149-154
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• 1998

Binding of urokinase-type plasminogen activator to its cellular receptor accelerate production of plasmin from plasminogen on the cell surface. Plasmin can digest extracellular matrix components and basement membranes through activating certain proMMPs, which is related to the invasiveness to the cells. Plasmin also acts the regulation of blood coagulation and relates closely to cardiovascular diseases such as stroke and coronary occlusion. Therefore, its inhibitors may be useful as antimetastatic agents and to the treatment of cardiovascular diseases. To search for plasmin inhibitors from plant resources, we screened plasmin inhibitory activities with 76 methanol extract of plant species. Among them, three plant samples showed strong inhibitory activities (>70%) and thirteen plant samples showed more than 50% inhibitory activities of plasmin. Their inhibitory activities were not directly related with uPA inhibitory activites and cell viability.

• The korean journal of orthodontics
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• v.45 no.4
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• pp.171-179
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• 2015

Objective: To compare dentoalveolar and skeletal changes produced by the pendulum appliance (PA) and the distal screw appliance (DS) in Class II patients. Methods: Forty-three patients (19 men, 24 women) with Class II malocclusion were retrospectively selected for the study. Twenty-four patients (mean age, $12.2{\pm}1.5years$) were treated with the PA, and 19 patients (mean age, $11.3{\pm}1.9years$) were treated with the DS. The mean distalization time was 7 months for the PA group and 9 months for the DS group. Lateral cephalograms were obtained at T1, before treatment, and at T2, the end of distalization. A Mann-Whitney U test was used for statistical comparisons of the two groups between T1 and T2. Results: PA and DS were equally effective in distalizing maxillary molars (4.7 mm and 4.2 mm, respectively) between T1 and T2; however, the maxillary first molars showed less distal tipping in the DS group than in the PA group ($3.2^{\circ}$ vs. $9.0^{\circ}$, respectively). Moreover, significant premolar anchorage loss (2.7 mm) and incisor proclination ($5.0^{\circ}$) were noted in the PA group, whereas premolar distal movement (1.9 mm) and no significant changes at the incisor ($0.1^{\circ}$) were observed in the DS group. No significant sagittal or vertical skeletal changes were detected between the two groups during the distalization phase. Conclusions: PA and DS seem to be equally effective in distalizing maxillary molars; however, greater distal molar tipping and premolar anchorage loss can be expected using PA.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.10 no.S_4
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• pp.197-206
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• 2001

The long-range transport mechanisms of Asian dust were analyzed based on the synoptic weather system and numerical simulation by using NCEP/NCAR reanalysis and TOMS data during the periods of 1996-2001. We classified the whole weather types of eastern Asia during spring and created the representative weather types during the yellow sand events using cluster analysis and weather charts for the last 6 years(1996~2001). These long-range transport mechanisms were related to various pressure patterns including high and low, trough and ridge, and upper-level fronts. Case studies of the yellow sand events have performed by the simulation of MM5 with meteorological elements such as the horizontal wind of u and v component, potential temperature, potential vorticity, and vertical circulation during the episodic days(2~8 March 2001). In addition, the origin of the long-range transport was examined with the estimation of backward trajectory using HYSPLIT4 Model. In this paper, we concluded that three weather types at 1000 hPa, 850 hPa, 500 hPa, and 300 hPa levels were classified respectively. The dominant features were the extending continental outflow from China to Korea at 1000 hPa and 850 hPa levels, the deep trough passage and cold advection at 500 hPa and 300 hPa levels during the yellow sand events. And also, we confirmed the existence of pola $r_tropical jets in the upper-level, the behavior of potential vorticity over Korea, the estimation of potential vorticity through vertical cross section, and the transport of yellow sand through backward trajectories.es.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1993.10a
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• pp.113-113
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• 1993

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.1458-1465
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• 2010

A lot of dredging and reclaming projects are recently under way in Korea for the efficient use of limiting land space. Saemanguem area is special case of reclaiming by dredged soil. In case of a confined disposal of dredged soils by a pump dredger, generally coarse grained soils are separated from fines with dropping at the near part of the pump dredger. This kind of seperation of fine contents could be a factor of liquefaction by earthquake. In Korea, recently, earthquakes with magnitude of 3.0 or higher are distinctively increasing in 1990. In this study, cyclic shear characterics of Saemanguem Dredged sand depending on fine content were analyzed. A series of undrained cyclic triaxial test with cyclic stress ratio ($\sigma_d/{2\sigma_{{\upsilon}c}}'$) were performed on both isotropic consolidated specimen and sand with fine contents of 0%, 5%, 15%, 30%, 40% under the effective vertical stress of 100kPa and 50% and 60%, 70% of relative density for fine content of 0%, respectively. In the test results, cyclic shear strength increased by increasing of cyclic stress ratio($\sigma_d/{2\sigma_{{\upsilon}c}}'$) with increasing the relative density at the same number of cyclic under the effective confining pressure of 100kPa. It is almost highest the double amplitude(DA) 1%, 3%, 5%, 7.5% and 10% at fine content of 15% between Cyclic stress ratio($\sigma_d/{2\sigma_{{\upsilon}c}}'$) value at cyclic number five and fine content. Number of cyclic is 30 under the effective vertical stress of 100kPa, 70% of relative density for fine content of 15%. when the cyclic stress ratio at each relative density was compared at cyclic number five, the double amplitude(DA) 1%, 3%, 5%, 7.5% and 10%, and the pore-pressure ratio (${\Delta}u/{\sigma'}_c$) 0.95 value were compared; under the relative density of 70% and the effective confining pressure of 100kPa. The pore-pressure ratio (${\Delta}u/{\sigma'}_c$) 0.95 value showed a similar trend to the double amplitude (DA) 5% line.