• Journal of Microbiology and Biotechnology
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• 제6권3호
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• pp.209-212
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• 1996

A two-step cooling technique has been developed for cryopreservation of suspension cultured Scutellaria baicalensis cells. Efficient regrowth of cryopreserved cells was obtained in cryoprotected cells with a mixture of 1.5 M glycerol and 0.4 M sucrose in Schenk and Hildebrandt medium without pretreatment in high osmotic medium. Optimum freezing conditions were found to be a cooling rate of $0.5^{\circ}C$ min from $4^{\circ}C$ to $-40^{\circ}C$, and then retaining samples at $-40^{\circ}C$ for 30 min prior to plunging into liquid nitrogen. A regrowth rate of approximately 95$%$ was obtained after three month storage in liquid nitrogen. Callus cultures established from the cryopreserved cells were found to produce the same patterns of flavonoid accumulation and retain their baicalin producing activity.

• 설비공학논문집
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• 제16권2호
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• pp.111-120
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• 2004

The optimal design method of indoor thermal environment using CFD coupled simulation and genetic algorithms (GA) is developed in this study. CFD could analyze the thermal environment considering the distribution of temperature, velocity, etc. in a room. Therefore, It would be appropriate to use CFD for the optimal design method considering their distribution. In this paper, the optimal design means the most appropriate boundary conditions of the room among the conditions where the design target of indoor therm environment is achieved. Two step optimal indoor thermal environment design method is proposed. It includes the GA for searching the optimal indoor thermal environment design. To examine the performance of this method, the optimal design of hybrid ventilation system, which uses the natural cross ventilation and the radiation-cooling panel is conducted. The optimal design which satisfies the design target (thermal comfort, minimum cooling load, minimum vertical temperature difference) is found using two step optimal design method.

• ALGAE
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• 제18권4호
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• pp.321-331
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• 2003

Cryopreservation of sporothalli of the red alga Porphyra (P. seriata, P. yezoensis, P. tenera, P. pseudolinearis and P. dentata) was performed by the two-step cooling method in liquid nitrogen. The algal samples were suspended in various cryoprotective solutions, and slowly cooled to -40$^{\circ}C$ in 4 hours using a programmed freezer. After the first slow cooling the suspensions with cryoprotectants were immediately immersed in liquid nitrogen. The suspension from the programmed freezer was thawed quickly by agitation of the vial in a water bath at 40°C. When ice in the suspension of cryogenic vial was mostly melted, the vial was transferred to an ice bath for complete melting of the residual ice. The cryoprotectants in the vial were washed off by gradual dilution with seawater. The viability of the cell was assessed with neutral red staining. The viability of Porphyra samples ranged 54.6-70.9% when the mixed suspension of 10% dimethylsulfoxide and 0.5 M sorbitol in 50% seawater used as a cryoprotectant.

• Bulletin of the Korean Chemical Society
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• 제21권3호
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• pp.281-290
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• 2000

We varied recombination method of fenetic algorithm (GA), i.e., crossover step, to compare efficiency of these methods, and to find more optimum GA method. In one method (A), we select two conformations(parents) to be recombined by systematic combination of lowest energy conformations, and in the other (B), we select them in a ratio proportional to the energy of the conformation. Second variation lies in how to select crossover point. First, we select it randomly(1). Second, we select range of residues where internal energy of the molecule does not vary for more than two residues, select randomly among such regions, and we select either thr first (2a) or the second residue (2b) from the N-terminal side, or the first (2c) or the second residue (2d) from the C-terminal side in the selected region for crossover point. Third, we select longest such hregion, and select such residue(as cases 2) (3a, 3b, 3c or 3d) of the region. These methods were tested in a 2-dimensionl lattice system for 8 different sequences (the same ones used by Unger and Moult., 1993). Results show that compared to Unger and Moult's result(UM) which corresponds to B-1 case, our B-1 case performed similarly in overall. There are many cases where our new methods performed better than UM for some different sequences. When cooling factor affecting higher energy conformation to be accepted in Monte Carlo step was reduced, our B-1 and other cases performed better than UM; we found lower energy conformers, and found same energy conformers in a smaller steps. We discuss importance of cooling factor variation in Monte Carlo simulations of protein folding for different proteins. (A) method tends to find the minimum conformer faster than (B) method, and (3) method is superior or at least equal to (1) method.

• Clinical and Experimental Reproductive Medicine
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• 제28권1호
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• pp.55-63
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• 2001

Objectives: This study was carried out to evaluate the effects of embryonic stage, cryoprotectant, and freezing-thawing method on the rates of survival and development of the cryopreserved mouse early embryo and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Materials and Methods: Two to eight cell embryos were obtained from oviducts of mated $F_1$ hybrid female mice superovulated by pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Two-step 1,2-propanediol (PROH), dimethylsulfoxide (DMSO) and 4-step PROH DMSO were used as cryoprotectant and dehydration and rehydration method of embryos, and slow-cooling or rapid-cooling method was used as frozen program. The survival rates of embryos were measured after thawing and rehydration, and the developmental rates of embryos were compared and observed during culturing embryos for 24, 48, 72, 96 hrs. Results: As for the survival and development rates of embryos according to embryonic stage, the survival rate of 2 cell stage in PROH and DMSO was significantly higher than 4-8 cell (64.5% versus 62.1 %,79.7% versus 73.2%) (p<0.01, p<0.01), but the development rates of 4-8 cell embryos in PROH and DMSO were significantly higher than 2 cell embryos for whole culture period (p<0.01) and the development rates of 4-8 cell embryos in PROH were significantly higher than 2 cell embryos in DMSO (p<0.01). As for the survival and development rates of embryos according to cryoprotectant, the survival rate of 2 cell embryo in DMSO was significantly higher than that in PROH (74.4% versus 64.5%) (p<0.01), whereas the development rate of embryos was not differ till 24 hrs. The developmen1 rate from morular to hatching blastocyst, however, was significantly higher in PROH than in DMSO during 48 hr (p<0.01). The survival rate of 4-8 cell embryo was 62.1% in PROH and 73.2% in DMSO. The development rates of embryo in PROH were significantly higher for whole culture periods (p<0.01, 0.05). In respect to the effect of freezing and thawing program on the survival and development rates of embryos, method of slow cooling and rapid thawing was more effective than that of rapid cooling and rapid thawing. Conclusions: The survival rate of embryo in 2 cell stage was higher than in 4-8 cell stage, and PROH appears more effective cryoprotectant than DMSO because PROH showed better development rates of embryos in 2 and 4-8 cell stage. Moreover, slow cooling and rapid thawing method was considered as the best cryopreservation program.

• 한국자기학회지
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• 제10권1호
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• pp.7-10
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• 2000

Co계 비정질합금을 400~46$0^{\circ}C$에서 열처리 한 후 냉각조건에 따른 자기특성의 변화를 조사하였다 42$0^{\circ}C$에서 30분 열처리 한 뒤 큐리점까지 노냉 후 수냉 시킨 경우 초투자율이 수냉 또는 노냉 했을 때보다 60-100% 정도 증가했다. 냉각속도에 따른 자기적 특성 변화를 내부음력 및 이온쌍의 규칙도(ordering) 관점에서 고찰하였다

• 한국작물학회지
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• 제45권3호
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• pp.211-215
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• 2000

시료의 지방산 분석은 먼저 지방의 추출이 선행되어야 하는데 이때에는 시료량 및 시약이 많이 들고 또한 분석시간이 많이 소요되므로 지방 추출 및 지방산의 에스테르화를 한 단계에서 실시하는 One step extraction/methylation법(방법 1)에 따라 주요 유료작물의 지방산 조성을 살펴보았다 이 방법은 methanol-heptane-benzene-DMP-H$_2$SO$_4$ 혼합용매를 사용하여 지방 추출 및 지방산 에스테르화를 단일층에서 일어나게 한것으로 기존의 sodium methoxide를 촉매로 한 methanolysis 법에 의한 지방산 분석 결과와 유의적인 차이를 보이지 않았다. 또한 이 혼합시약으로 이미 추출된 지방을 대상으로 실험하였을 때에도(방법 2) 기존방법과 유의적인 차이가 없었다. 따라서 이 One step extraction/methylation법은 시료가 생체이든 추출된 지방이든 그 과정이 단순하고 시간과 노력이 훨씬 적게 소요되어 지방산 분석에 널리 이용될 수 있을 것이며, 특히 1회에 많은 시료를 처리하고자 할 때 더욱 분석효율이 높을 것으로 기대되었다

• 한국분무공학회지
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• 제19권1호
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• pp.19-24
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• 2014

In this study, the cooling performance of a liquid CPU cooler using the water/propylene glycol(PG)-based $Al_2O_3$ nanofluids is experimentally investigated. Water/PG-based $Al_2O_3$ nanofluids are manufactured by two-step method with ultrasonic energy for 10 hours. The volume fractions of the nanofluids are 0.25% and 0.35%. Thermal conductivity and viscosity of the nanofluids are measured to theoretically predict the thermal performance of the liquid CPU cooler using performance factor. Performance factor results indicate that the cooling performance of the liquid CPU cooler can be improved using the manufactured nanofluids. To evaluate the cooling performance of the liquid CPU cooler experimentally, temperature differences between ambient air and heater are measured for base fluid and nanofluids respectively. Based on the results, it is shown that performance of the liquid CPU cooler using $Al_2O_3$ nanofluids is improved maximum up to 8.6% at 0.25 Vol.%.

• Journal of Radiation Protection and Research
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• 제40권2호
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• pp.92-100
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• 2015

경수로 사용후핵연료 건식 중간저장시설의 방사선영향평가 효율성 개선을 목적으로 '선원항 지정방법에 따른 민감도 평가', '2-Step 계산'기법 개발 및 '냉각기간 이득효과' 적용에 따른 방사선 영향평가를 수행하였다. 본 연구에서는 저장건물의 용기배열 순서에 따라 순차적으로 선원항을 지정하여 직접선량에 미치는 민감도를 평가하였으며, 차폐건물 외벽에서의 방사선량은 내벽과 인접한 최근접 2개 열에 의한 영향이 지배적임을 확인하였다. 또한, 저장시설에 차폐 건물이 도입될 경우, 막대한 전산해석 시간을 감소시키기 위해 '2-Step 계산'기법을 수립하여 평가한 결과는 절반가량의 해석시간으로 직접(1-Step) 계산결과와 유사한 결과를 도출하였다. 마지막으로, 저장시설에 순차적으로 저장되는 저장용기의 보관기간을 사용후핵연료의 실제 냉각기간을 적용하면 건물 외벽에서의 방사선량이 냉각기간을 모두 동일하게 설정한 계산값에 비해 40% 정도 낮게 평가됨을 확인하였다. 본 연구는 중간저장시설의 방사선 영향평가를 위한 몬테칼로 차폐해석 방법의 효율성을 향상시키고자 수행되었으며, 좀 더 다양한 사례에 대한 평가를 통하여 신뢰성을 향상시킨다면 저장시설의 설계 및 부지경계 기준설정에 활용할 수 있을 것이다.

• 설비공학논문집
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• 제14권11호
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• pp.914-922
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• 2002

Thermo-acoustic waves can be thermally generated in a compressible flow field by rapid heating and cooling, and chemical reaction near the boundary walls. This mechanism is very important in the space environment in which natural convection does not exist. Also this may be a significant factor for heat transfer when the fluids are close to the thermodynamic critical point. In this study, the generation and transmission characteristics of thermo-acoustic waves in an air-filled confined domain with two-step pulsed heating are studied numerically. The governing equations are discretized using control volume method, and are solved using PISO algorithm and second-order upwind scheme. For the purpose of stable solution, time step was set to the order of $1\times10_-9s,\;and\;grids\;are\;50\times2000$. Results show that temperature and pressure distributions of fluid near the boundary wall subjected to a rapid heating are increased abruptly, and the induced thermo-acoustic wave propagates through the fluid until it decays due to viscous and heat dissipation. Pressure waves have sharp front shape and decay with a long tail in the case of step heating, but these waves have sharp pin shape in the case of pulsed heating.