• Journal of Environmental Science International
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• v.27 no.1
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• pp.1-10
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• 2018

A continuous process of persulfate oxidation and citric acid washing was investigated for ex-situ remediation of complex contaminated soil containing total recoverable petroleum hydrocarbons (TRPHs) and heavy metals (Cu, Pb, and Zn). The batch experiment results showed that TRPHs could be degraded by $Fe^{2+}$ activated persulfate oxidation and that heavy metals could be removed by washing with citric acid. For efficient remediation of the complex contaminated soil, two-stage and three-stage processes were evaluated. Removal efficiency of the two-stage process (persulfate oxidation - citric acid washing) was 83% for TRPHs and 49%, 53%, 24% for Cu, Zn, and Pb, respectively. To improve the removal efficiency, a three-stage process was also tested; case A) water washing - persulfate oxidation - citirc acid washing and case B) persulfate oxidation - citric acid washing (1) - citric acid washing (2). In case A, 63% of TRPHs, 73% of Cu, 60% of Zn, and 55% of Pb were removed, while the removal efficiencies of TRPHs, Cu, Pb, and Zn were 24%, 68%, 62%, and 59% in case B, respectively. The results indicated that case A was better than case B. The three-stage process was more effective than the two-stage process for the remediation of complex-contaminated soil in therms of overall removal efficiency.

• Microbiology and Biotechnology Letters
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• v.2 no.1
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• pp.29-35
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• 1974

We have studied the applicability of the principles and inherent advantages of the two-stage dontinuous uclture technique to an enzyme process for the purpose of improving and optimizing the productivity of 3-ketosteroid-delta-1-dehydrogenase. By using a two-stage continuous culture system, the growth st ageand enzyme produdtion stage are separated. In each stage an optimal set of toperaing conditions was determined, and this was tested for feasibility for the period of 10 days. During this period, at least 70％ of the maximum enzyme productivity could be maintained. The important design parameters studied are: (1) optimal specific growth rate in the first stage which corresponds to the maximal cell productivity, (2) the optimal dilution rate in the second stage which in turn determines the size of second stage fermentor and the mean residence time of cells in the second stage, (3) cell concentration in both stages, add (4) the specific enzyme productivity and enzyme productivity of the second stage. In addition, by using two-stage continuous culture system we have been able to reduce or eliminate the effect of catabolite repression due to high medium concentration and the adverse effect of the solvent used to dissolve the inducer. We have found the balance between the opposing effects of induction and repression in the second stage judging from the observation that the enzyme productivity goes through a maximum.

• KSBB Journal
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• v.11 no.1
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• pp.58-64
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• 1996

The purpose of this study is to develop a continuous process for sorbitol production using Zymomonas mobilis immobilized in K-carra-geenan. The glutaraldehyde cross-linking of toluene-treated cells immobilized in alginate or chitin showed high enzyme stability for long period. However, loss of enzyme activity was observed at 23% during 210h. In order to investigate the stability of glucose-fructose oxidoreductase of cethyltrimethylammoniumbromide (CT AB) treated cells, the long term continuous process was carried out with Z. mobilis immobilized in K-carrageenan in the continuous stirred tank reactor(CSTR) and the packed bed reactor. The continuous production of sorbitol with the immobilized CT AB permeabilized cells in packed bed reactor was more stable than in CSTR. Two stage continuous process with CT AB treated cells of Z. mobilis immobilized in K-carrageenan was carried out at various dilution rates. At the first stage, the productivity was increased up to 15 g/ $\ell$ -h as dilution rate increased and decreased over 0.32$h^{-1}$ of dilution rate. Similarly, maximum productivity obtained at the second stage was 22g/$\ell$ -h at 0.32$h^{-1}$

• KSBB Journal
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• v.7 no.4
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• pp.308-316
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• 1992

Two-stage continuous culture system has been studied intensively to maximize the productivity of a cloned gene product in unstable recombinant microorganism. As an effort to optimize the two-stage process, transient behavior of the second-stage was studied theoretically as well as experimentally using Escherichia coli Kl2$\delta$Hl$\delta$trp. A mathematical model describing the transient response to a step change in dilution rate was developed based on the assumption that the adaptation rate of cell growth is proportional to the available growth potential, which is defined as the difference in dilution rates between before and after shift-up. The kinetic parameters appearing in the model equations were the dimensionless step increase in growth rate($\alpha$) and the adaptation rate constant(k). These parameters were evaluated for various dilution rates and temperatures by washout method. This relatively simple adaptation model could predict the specific growth rate of the second-stage successfully. Advantage and disadvantage of the proposed model are also discussed.

• KSBB Journal
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• v.15 no.5
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• pp.489-496
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• 2000

Several culture systems including batch, two-stage CSTR, semi-fed batch, and two-stage cyclic fed-batch were investigated for the efficient production of the Fab fraction of PDC-E2 specific human monoclonal antibody using high cell density recombinant E. coli. A two-phase batch system and a two-stage continuous system were examined to overcome plasmid instability problems, by separating the growth and the production stages. The cell density and productivity of the two-stage continuous culture was better than that of the two-phase batch fermentation. In the two-stage continuous culture system with DO-stat, the cell growth and the productivity were superior to those of the system without the DO control. Also, almost total plasmid stability was maintained in the two-stage continuous culture system. Modified M9 medium was selected as an optimum feeding medium for the fed-batch process, and the optimum C/N ratio determined to be 2:3. The optimum feeding rate was $0.6g/\ell/hr$ for a constant feeding strategy in semi-fed batch system. When the feeding medium was fed by pulsing, it was observed that more frequent pulsing resulted in improved cell growth. The linear feeding method was the most efficient of the various feeding methods tested. Finally, high cell density culture using a two-stage cyclic fed batch system with pH-stat was tried because the linear feeding method showed limitations in terms of obtaining high cell densities, and a cell density of $54 g/\ell$ was achieved. It was concluded that the two-stage cyclic fed batch system was the most efficient system for high cell density culture of the systems tested. However, productivity improvements were lower than expected due to the extremely high accumulations of acetate, although the low levels of residual glucose were maintained.

• International conference on construction engineering and project management
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• 2007.03a
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• pp.279-288
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• 2007

Attempts to achieve a higher productivity have led studies to focus on process improvement. Information has been found as an essential element for process improvement. This research has introduced and focused on two types of information, namely: related jobsite information along the process and feedback information. Related jobsite information along the process which needs to be processed and delivered in a timely manner, accurate, and real time is required to streamline the decision making process. Whereas feedback information about process' current practices which have to be captured and stored is a useful for continuous improvement in identifying the problem origin and determining corrective action. In the current practices, although these two types of information are essential for process improvement, construction process has faced barriers in obtaining that information. Therefore, this research will propose a new information system to overcome the aforementioned barriers. The new information system consists of RFID as an automatic identification and data collection device integrated with database to support construction processes. The new system attempts to provide related jobsite information along the process and feedback information to support decision making process and continuous process improvement respectively. A case study of prefabrication installation process in housing projects has been selected to be implemented in conceptual model of RFID application in construction industry. Conceptual model will be presented in this paper as an initial stage of this ongoing research. Expected outcomes of the new system and future works will be discussed briefly.

• Journal of Power System Engineering
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• v.7 no.4
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• pp.12-18
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• 2003

Various measures have been tried to reduce the NOx emission from diesel engine, but with partial success because the mechanisms of NOx and PM formations appear to have trade-off relation between each other. Therefore it has been known to be difficult to reduce NOx emission and PM emission simultaneously. Two stage combustion method i,e. a combustion process which has rich combustion stage and lean combustion stage one by one, has been developed successfully to reduce NOx formation in the continuous combustion chambers such as in the boilers. But until yet it is not successful to apply the same method in intermittent combustion chamber like in the diesel engine cylinder, as it was, only several research works were carried out. In this study, devised was a uniquely shaped combustion chamber with reformed piston crown intended to keep fuel-rich condition during early stage of combustion and fuel-lean condition during next stage. It was found that the NOx emission decreased significantly at various conditions of operation with the two stage combustion type engines of PR20 type, but other values such as smoke, CO and specific fuel consumption deteriorated as usual.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.1
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• pp.122-129
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• 2008

Two-photon stereolithography is recognized as a promising process for the fabrication of three-dimensional (3D) microstructures with 100 nm resolution. Generally, beam-scanning system has been used in the conventional process of two-photon stereolithography, which is limited to the fabrication of micro-prototypes in small area of several tens micrometers. For the applications to 3D high-functional micro-devices, the fabrication area of the process is required to be enlarged. In this paper, large-area two-photon stereolithography (L-TPS) employing stage scanning system has been developed. Continuous scanning method is suggested to improve the fabrication speed and parameter study is conducted. An objective lens of high numerical aperture (N.A.) and high strength material were employed in this system. Through this work, 3D microstructures of $600*600*100\;{\mu}m$ were fabricated.

• Journal of Global Scholars of Marketing Science
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• v.13
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• pp.135-158
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• 2004

On-line consumers have some visit behavior characteristics when they visit internet-shopping mall between visit-stage and purchase-stage. Therefore, information of on-line consumers have influenced on internet-shopping mall's profitabilities at site manager's perspectives. For examples, Are any on-line consumers continuous visiting under any situations? Or are any on-line consumers purchased on any specific internet-shopping mall? Expecially in this paper, researcher tried to understand visit behavioral characteristics of on-line consumers using two-stage mixture regression analysis. Throughout this process, it could be proposed method, which could be reinforced competitiveness of internet-shopping mall by segmental decision-making method. Additionally, it is expected that visit behavioral characteristics' information could be supplied strategic implications between visit-stage and purchase-stage Throughout empirical test it could be proved two-stage decision-making process, which decision-making process of on-line consumers would be processed visit-stage and purchase-stage. In this study, researcher proposed suitable response strategy after understanding visiting behavioral characteristics of on-line consumers. This paper has some academical contributions, which visit behavioral characteristics of on-line consumers could be grasped the meaning by site stickiness and navigation pattern.

• Journal of Information Technology Services
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• v.21 no.6
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• pp.91-116
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• 2022

This research shows how the perception of digital shadow work varies depending on the user's period of use of digital services in applications, which are key areas of digital services. The purpose of this research is to investigate how the digital shadow work type changes over time. This research adopted a qualitative research method for location-based service "help application" users and examined the change in perception from the app service acceptance stage to the app service continuous use stage with two-times interviews of ten users by a longitudinal research design. This study conducted a longitudinal analysis focusing on how digital shadow work occurs in the application subscription process, exploring phenomenologically how the type changes throughout the process over time.