• Title/Summary/Keyword: two batch method

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Dyeing Protein Fiber to Green Color Using Natural Mugwort and Indigo (천연 쑥과 쪽을 이용한 단백질 섬유의 녹색 염색)

  • Yoo, Hye-Ja
    • Journal of the Korean Home Economics Association
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    • v.45 no.4
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    • pp.53-59
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    • 2007
  • We need to diversify the colors by natural dyeing for promotion and extention of the natural dyes market, because natural dyestuffs have the limitation the number of the colors to express, compare to synthetic dyestuffs. It was investigated that wool and silk fabrics could be dyed to green colors using natural mugwort and indigo as one of color diversification, in order to express green color that is difficult to be shown by natural dyeing. The mugwort dyebath was prepared to concentration of $25{\sim}100g/l$ using dried mugwort plant and indigo dyebath was prepared to concentration of $5{\sim}20g/l$ using natural indigo powder. Wool fabrics and silk fabrics were dyed to green(GY, G, BG in Munsell color wheel) by two batch methods using the mugwort and indigo dyebaths. the mugwort dyeing was applied at $80^{\circ}C$ for 20minutes and indigo dyeing applied for $5{\sim}7$ minutes in room temperature. The colorfastness to drycleaning and abrasion of the dyed fabrics were shown good as grade 4-5 or 5.

Kinetics of Converting Single Chain Urokinase Type Plasminogen Activator into Two Chain Plasminogen Activator in Cultivating HEK Cells with Low Serum Containing Medium (저혈청 배지에서 생산된 scu-PA의 tc-PA로의 전환에 관한 연구)

  • 김영남;김현구
    • KSBB Journal
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    • v.9 no.1
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    • pp.48-54
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    • 1994
  • A modified amidolytic assay and a fibrin plate method were used to accurately measure the concentration of single chain urokinase type plasminogen activator (scu-PA) and two-chain urokinase type plasminogen activator (tc-PA) in the spent media. $1.65{\times}10^6$(viable cells/ml) of maximum cell density and 1670(IU/ml) of scu-PA concentration were obtained in 1% serum containing medium. The overall conversion ratio from scu-PA to tc-PA was less than 10%. In the results of batch cultivation in a spinner vessel, $4.43{\times}10^6(total cells/ml)$ of maximum cell density and 1560(IU/ml) of scu-PA concentration was observed. The maximum scu-PA concentration and specific scu-PA Productivity were obtained in 1760(IU/ml) and $3.13{\times}10^{-4}(IU/cell)$, respectively, from perfusion cultivation. The conveysion ratios from batch, fed-batch and perfusion cultivations were less than 12%, which means that about 90% of scu-PA secreted from the cells can be maintained during the cultivations.

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The Importance of Reaction Mechanisms in Interpreting the Arsenic Reactive Transport of FeS-coated Sand Column

  • Han, Young-Soo;Demond, Avery H.;Hayes, Kim F.
    • Journal of Soil and Groundwater Environment
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    • v.20 no.5
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    • pp.1-10
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    • 2015
  • FeS, as a natural reduced iron mineral, has been recognized to be a viable reactive material for As(III) sequestration in natural and engineered systems. In this study, FeS-coated sand packed columns were tested to evaluate the As(III) removal capacities under anaerobic conditions at pH 5, 7 and 9. The column obtained As(III) removal capacity was then compared with the capacity result obtained from batch reactors. In the comparison, two different approaches were used. The first approach was used the total As(III) removal capacity which method was proved to be useful for interpreting pH 5 system. The second approach was used to consider sorption non-linearity and proved to be useful for interpreting the pH 9. The results demonstrated that a mechanistic understanding of the different removal processes at different pH conditions is important to interpret the column experimental results. At pH 5, where the precipitation of arsenic sulfide plays the major role in the removal of arsenic, the column shows a greater removal efficiency than the batch system due to the continuous dissolution of sulfide and precipitation of arsenic sulfide. At pH 9, where adsorption mainly governs the arsenic removal, the sorption nonlinearity should be considered in the estimation of the column capacity. This study highlighted the importance of understanding reaction mechanism to predict column performance using batch-obtained experimental results.

LEU+ loaded APR1400 using accident tolerant fuel cladding for 24-month two-batch fuel management scheme

  • Husam Khalefih;Taesuk Oh;Yunseok Jeong;Yonghee Kim
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2578-2590
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    • 2023
  • In this work, a 24-month two-batch fuel management strategy for the APR1400 using LEU + has been investigated, where enrichments of 5.9 and 5.2 w/o are utilized in lieu of the conventional 4-5 w/o UO2 fuel. In addition, an Accident Tolerant Fuel (ATF) clad based on the swaging technology is applied to APR1400 fuel assemblies. In this special ATF clad design, both outer and inner SS316 layers protect the conventional zircaloy clad. Erbia (Er2O3) is introduced as a burnable absorber with two-fold goals to lower the critical boron concentration in the long-cycle LEU + loaded core as well as to handle the LEU + fuel in the existing front-end fuel facilities without renewing the license. Two types of fuel assemblies with different loading of gadolinia (Gd2O3) are considered to control both the reactivity and the core radial power distribution. The erbia burnable absorber is uniformly admixed with UO2 in all fuel pins except for the gadolinia-bearing ones. In this study, two core designs were devised with different erbia loading, and core performance and safety parameters were evaluated for each case in comparison with a core design without any burnable absorbers. The core analysis was done using the two-step method. First, cross-sections are generated by the SERPENT 2 Monte Carlo code, and the 3-D neutronic analysis is performed with an in-house multi-physics nodal code KANT.

Formation, Properties and Antimicrobial Activities of Cotton Xanthate-Cu(II)-Homosulfamine Complex

  • Ha, Neung-Jin;Jung, Yun-Jin;Lee, Jeoung-Soo;Kim, Youn-Taeg;Kim, Young-Mi
    • Archives of Pharmacal Research
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    • v.21 no.5
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    • pp.570-575
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    • 1998
  • To develop a cotton derivatives with prolonged antimicrobial activities, homosulfamine (Hs) was coupled to cotton xanthate (CX) via chelate bond in the presence of Cu(II) ion by one- and two-bath processes. In one-bath process, CX was treated with Cu(II)-Hs solution. In two-bath process, CX was treated with Cu(II) ion solution to produce CX-Cu(II) complex, which was isolated and treated in turn with Hs solution. Effects of concentration, Cu(II)/Hs ratio, and pH on the binding of Hs were investigated at $10{\circ}C$ . In one-bath process, binding of hs took place readily with optimum pH around 5-6. the amount of binding increased to give a maximum within 5 min and decreased slowly to establish an equilibrium withhin an hour. In two-bath process, binding of Hs was much lower than that of one-bath process. Release of Hs from CX-Cu(II)-Hs was investigated by batch and flow method. antimicrobial activiteis of CX-Cu(II)-Hs was investigated by batch and flow method. Antimicrobial activities of CX-Cu(II)-Hs were tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli and it showed prolonged activity compared to that of free Hs.

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Detection of Denitrification Completion Using Pattern Matching Method in Sequencing Batch Reactor(SBR) (연속회분식반응기에서 패턴매칭방법을 이용한 탈질완료 감지 알고리즘 개발)

  • Kim, Ye-Jin;Ahn, Yu-Ga;Shin, Jung-Phil;Kim, Chang-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.8
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    • pp.944-949
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    • 2007
  • The profiles of on-line sensors such as DO, ORP and pH can provide useful information about pollutant removal reaction in sequencing batch reactor. For detection of denitrification completion, the nitrate hee point from ORP profile has been considered as a main indicator of denitrification completion. However, many researchers pointed out that the nitrate knee usually disappeared been the progress of denitrification is so fast and it makes the fault at detection of denitrification completion. In this paper, dynamic time warping(DTW) method and discriminant analysis were used to detect and isolate the profiles of two cases, denitrification completed and uncompleted. As the results, proposed methods can detect state of denitrification successfully.

Desorption Kinetics and Removal Characteristics of Pb-Contaminated Soil by the Soil Washing Method: Mixing Ratios and Particle Sizes

  • Lee, Yun-Hee;Oa, Seong-Wook
    • Environmental Engineering Research
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    • v.17 no.3
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    • pp.145-150
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    • 2012
  • Pb-contaminated soil at a clay shooting range was analyzed by the sequential extraction method to identify metal binding properties in terms of detrital and non-detrital forms of the soil. Most of the metals in the soils existed as non-detrital forms, exchangeable and carbonate-bound forms, which could be easily released from the soil by a washing method. Therefore, the characteristics of Pb desorption for remediation of the Pb-contaminated soil were evaluated using hydrochloric acid (HCl) by a washing method. Batch experiments were performed to identify the factors influencing extraction efficiency. The effects of the solid to liquid (S/L) ratio (1:2, 1:3, and 1:4), soil particle size, and extraction time on the removal capacity of Pb by HCl were evaluated. Soil samples were collected from two different areas: a slope area (SA) and a land area (LA) at the field. As results, the optimal conditions at 2.8 to 0.075 mm of particle size were 1:3 of the S/L ratio and 10 min of extraction time for SA, and 1:4 of the S/L ratio and 5 min of extraction time for LA. The characteristics of Pb desorption were adequately described by two-reaction kinetic models.

DISCRETE-TIME $Geo^X/G/l$ QUEUE WITH PLACE RESERVATION DISCIPLINE

  • Lee Yu-Tae
    • Journal of applied mathematics & informatics
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    • v.22 no.1_2
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    • pp.453-460
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    • 2006
  • A discrete-time priority queueing system with place reservation discipline is studied, in which two different types of packets arrive according to batch geometric streams. It is assumed that there is a reserved place in the queue. Whenever a high-priority packet enters the queue, it will seize the reserved place and make a new reservation at the end of the queue. Low-priority arrivals take place at the end of the queue in the usual way. Using the probability generating function method, the joint distribution of system state and the delay distribution for each type are obtained.

Synthesis of Model Microspheres and Adsorption Study of Bovine Albumin (모델 Microspheres의 합성 및 Bovine Albumin의 흡착)

  • 박영준;윤정열
    • Journal of Biomedical Engineering Research
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    • v.14 no.3
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    • pp.209-220
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    • 1993
  • Microspheres are expected to be applied to biomedical areas such as solid-phase immunoassays, drug delivery systems, Immunomagnetic cell separation. To synthesize micro-spheres for biomedical application, "two stage shot growth method" was developed. The uniformity ratio or synthesized microspheres was always smatter than 1.05. And the surface charge density (or the number of ionizable functional groups) of the microspheres synthesized by "two stage shot growth method" was 6-13 times higher than thats of the ml crospheres synthesized by conventional seeded batch copolymerization. As a previous step for biomedical application, adsorption experiments of bovine albumin on microspheres were carried out under various conditions. The maximum adsorbed amount was obtained in the neighborhood of pH 4.5. Isoelectric point of bovine albumin Is pH 5.0, so experimental result shows that it shifted to acid area. The adsorption Isotherm was obtained, the plateau region was always reached at 2.Og/L (bulk concentration of bovine albumin ) . The effect of the kind and the amount of surface functional group was also examined.p was also examined.

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초전도 NMR-CT의 영상 원리 및 그 응용

  • 조장희
    • Journal of Biomedical Engineering Research
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    • v.7 no.2
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    • pp.183-190
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    • 1986
  • Microspheres are expected to be applied to biomedical areas such as solid-phase immunoassays, drug delivery systems, immunomagnetic cell separation. To synthesize microspheres for biomedical application, "two stage shot growth method" was developed. The uniformity ratio of synthesized microspheres was always smaller than 1.05. And the surface charge density (or the number of ionizable functional groups) of the microspheres synthesized by "two stage shot growth method" was 6~13 times higher than that of the microspheres synthesized by conventional seeded batch copolymerization. As a previous step for biomedical application, adsorption experiments of bovine albumin on microspheres were carried out under various conditions. The maximum adsorbed amount was obtained in the neighborhood of pH 4.5. Isoelectric point of bovine albumin is pH 5.0, so experimental result shows that it shifted to acid area. The adsorption isotherm was obtained, the plateau region was always reached at 2.Og/L (bulk concentration of bovine albumin).The effect of the kind and the amount of surface functional group was also examined.p was also examined.

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