• Journal of the Korean Association of Geographic Information Studies
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• v.12 no.3
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• pp.45-55
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• 2009

This study was intended to increase efficiency of traffic flow management on intersection. The result suggested to establish a left-turn at own risk lane to increase efficiency of traffic flow on intersection. The scope of the research was to investigate the geometric structure of a signal-controlled intersection, traffic volume(density) with respect to directions and traffic signal display, and to select a signalling intersection into which a car waiting for a traffic signal enters by adjusting the display sequence of traffic signal. The delay with respect to directions and for the whole intersection was compared for the current situation and an improvement plan. Using TSIS, a traffic analysis package, the traffic situation on an intersection was investigated. Based on the simulation result for Seok-Jeon intersection in Ma-San selected from the field investigation of intersections to which an improvement plans would be applicable, the waiting time in the direction without a entering traffic signal was decreased to be 78.6 seconds per car and that of the direction expecting the increase of waiting time was increased by 4 seconds per car only. It was confirmed that the waiting time for the whole intersection was improved.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.10 no.1
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• pp.27-41
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• 2011

This research proposes new tram signal coordination model, called MAXBAND MILP-Tram for a passive tram signal priority strategy. The proposed model was formulated based on the MAXBAND model that was a traditional arterial signal optimization model. The model could calculate the bandwidth solutions for both general-purpose-lane traffic and median-tram-lane traffic. Lower progression speed are applied for the tram traffic considering lower running speed and dwell time at the stations. A phase sequence procedure determines the green times and left-turn phase sequences for tram traffic in median tram lane. To estimate the performance of the MILP-Tram model, the control delay of trams were estimated using the micro simulation model, VISSIM. The analysis results showed 57 percent decrease of the tram compared to the conventional signal timing model. The delay for car, however, increased 18 percent. The sensitivity analysis indicated that the passive tram signal priority strategy using the offset and phase sequence optimization was effective in reducing the person delay under the congested traffic condition.

• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.117-123
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• 2003

For screening of autoregulator receptor gene from Saccharopolyspora erythraea, PCR was performed with primers of receptor gene designed on the basis of amino acid sequences of autoregulator receptor proteins with known function. PCR products were subcloned into the BamHI site of pUC19 and transformed into the E. coli DH5$\alpha$. The isolated plasmid from transformant contained the fragment of 120 bp, which was detected on 2% gel after BamHI treatment. The insert, 120 bp PCR product, was confirmed as the expected internal segment of gene encoding autoregulator receptor protein by sequencing. Southern and colony hybridization using Saccha. erythraea chromosomal DNA were performed with the insert as probe. The plasmid (pEsg) having 3.2 kbp SacI DNA fragment from Saccha. erythraea is obtained. The 3.2 kbp SacI DNA fragment was sequenced by the dye terminator sequencing. The nucleotide sequence data was analyzed with GENETYX-WIN (ver 3.2) computer program and DNA database. frame analyses of the nucleotide sequence revealed a gene encoding autoregulator receptor protein which is a region including KpnI and SalI sites on 3.2 kbp SacI DNA fragment. The autoregulator receptor protein consisting of 205 amino acid was named EsgR by author. In comparison with known autoregulator receptor proteins, homology of EsgR showed above 30%.

• BMB Reports
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• v.40 no.5
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• pp.740-748
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• 2007

To gain insight into the structure and function of repressor proteins of bacteriophages of gram-positive bacteria, repressor of temperate Staphylococcus aureus phage ${\phi}11$ was undertaken as a model system here and purified as an N-terminal histidine-tagged variant (His-CI) by affinity chromatography. A ~19 kDa protein copurified with intact His-CI (~ 30 kDa) at low level was resulted most possibly due to partial cleavage at its Ala-Gly site. At ~10 nM and higher concentrations, His-CI forms significant amount of dimers in solution. There are two repressor binding sites in ${\phi}11$ cI-cro intergenic region and binding to two sites occurs possibly by a cooperative manner. Two sites dissected by HincII digestion were designated operators $O_L$ and $O_R$, respectively. Equilibrium binding studies indicate that His-CI binds to $O_R$ with a little more strongly than $O_L$ and binding species is probably dimeric in nature. Interestingly His-CI binding affinity reduces drastically at elevated temperatures ($32-42^{\circ}C$). Both $O_L$ and $O_R$ harbor a nearly identical inverted repeat and studies show that ${\phi}11$ repressor binds to each repeat efficiently. Additional analyses indicate that ${\phi}11$ repressor, like $\lambda$ repressor, harbors an N-terminal domain and a C-terminal domain which are separated by a hinge region. Secondary structure of ${\phi}11$ CI even nearly resembles to that of $\lambda$ phage repressor though they differ at sequence level. The putative N-terminal HTH (helix-turn-helix) motif of ${\phi}11$ repressor belongs to the HTH -XRE-family of proteins and shows significant identity to the HTH motifs of some proteins of evolutionary distant organisms but not to HTH motifs of most S. aureus phage repressors.

• International Journal of Internet, Broadcasting and Communication
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• v.12 no.1
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• pp.67-72
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• 2020

The fifth generation (5G) mobile communication has an impact on the human life over the whole world, nowadays, through the artificial intelligence (AI) and the internet of things (IoT). The low latency of the 5G new radio (NR) access is implemented by the state-of-the art technologies, such as non-orthogonal multiple access (NOMA). This paper investigates a practical issue that in NOMA, for the practical channel models, such as fading channel environments, the successive interference cancellation (SIC) should be performed on the stronger channel users with low power allocation. Only if the SIC is performed on the user with the stronger channel gain, NOMA performs better than orthogonal multiple access (OMA). Otherwise, NOMA performs worse than OMA. Such the superiority requirement can be easily implemented for the channel being static or slow varying, compared to the block interval time. However, most mobile channels experience fading. And symbol by symbol channel estimations and in turn each symbol time, selections of the SIC-performing user look infeasible in the practical environments. Then practically the block of symbols uses the single channel estimation, which is obtained by the training sequence at the head of the block. In this case, not all the symbol times the SIC is performed on the stronger channel user. Sometimes, we do perform the SIC on the weaker channel user; such cases, NOMA performs worse than OMA. Thus, we can say that by what percent NOMA is better than OMA. This paper calculates analytically the percentage by which NOMA performs better than OMA in the practical mobile communication systems. We show analytically that the percentage for NOMA being better than OMA is only the function of the ratio of the stronger channel gain variance to weaker. In result, not always, but almost time, NOMA could perform better than OMA.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.7 no.5
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• pp.418-429
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• 1996

The number of bits per message and the number of tones in the frequency-hopping sequence are determined by the available bandwidth and the data rate of each user. These parameters in turn determine the tone duration which strongly influences the vulnerability of the system to transmission distortions. In this paper, an address code which is assigned to each individual user was employed in order to reduce the collisions or hit. Also the frequency band is divided into several subbands and each user transmits multitone frequency per subband per chip. And the new system which is to increase the duration of each tone by increasing the total number of system frequencies that has been proposed. It is found that an optimum value bit, tone, number of frequencies per chirp can improve the err performance. This flexibility slightly increases maximum efficiecy and makes the the system less vulnerable to multipath delay. So, It is found that as the nuber of user increased 50%, the efficiency as a tuncion of the bandwidth to user'rate ratio improve 20%.

• Reproductive and Developmental Biology
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• v.33 no.1
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• pp.63-69
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• 2009

In this study we tried to construct a more efficient tetracycline-inducible gene expression system by replacing previous key elements with more advance ones. At the beginning, we substituted PGK (phophoglycerate kinase) promoter for CMV (cytomegalovirus) promoter to control "$rtTA2^sM2$" which has been known for high induction efficiency in response to tetracycline. With this modification, expression of the EGFP marker gene under the induction condition was significantly increased. Next, we replaced "TRE" fragment with a modified version named "TRE-tighf" which has been reported to have higher affinity and specificity to the transactivator by minor base change of the "TRE" DNA fragment sequence. Use of "TRE-tighf" instead of "TRE" resulted in more than 10 fold increment in terms of induction efficiency and significant decrement of background expression in non-inducible condition. By combining PGK promoter and "TRE-tight" fragment, we could upgrade previous tetracycline-inducible system to show more stringent turn on/off gene switch ability and stronger expression of the gene of our interest. Use of this newly developed system must be very helpful to the studies of gene expression, especially to the transgenic animal study in which non-controllable constitutive expression of the transgene has been one of the urgent problems to be solved.

• Journal of the Korean Magnetic Resonance Society
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• v.6 no.1
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• pp.54-68
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• 2002

PEBP2/CBF (Polyomavirus Enhancer-core Binding Protein 2/Core Binding Factor), represents a new family of heterodimeric transcription factor. Those members play important roles in hematopoiesis and osteogenesis in mouse and human. PEBP2/CBF is a sequence-specific DNA binding protein. Each member of the PEBP2/CBF family of transcription factors is composed of two subunits, ${\alpha}$ and ${\beta}$. The evolutionarily conserved 128 amino acid region in ${\alpha}$ subunit has been called the Runt domain, which harbors two different activities, the ability to bind DNA and interact with the ${\beta}$ subunit. Recently, cDNA clones encoding the C. elegans Runt domain were isolated by screening a cDNA library. This gene was referred to run (Runt homologous gene). In this study, the basic experiments for the structural characterization of RUN protein were performed using spectroscopic methods. We have identified the structural properties of RUN using bioinformatics, CD and NMR. The limit temperature of the structural stability was up to 60$^{\circ}C$ with irreversible thermal process, and the structure of RUN seems to adopt ${\alpha}$ helices and one or more ${\beta}$ sheet or turn. The degree of NMR peak dispersion and intensity was increased by addition of glycine. Therefore, glycine could be used to alleviate the aggregation property of RUN in NMR experiment.

• BMB Reports
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• v.38 no.5
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• pp.624-631
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• 2005

Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the main diseases to mankind. It is urgent to discover novel drug targets for appropriate antimicrobial agents against this human pathogen. The shikimate pathway is onsidered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammalian cells. The Mycobacterium tuberculosis aroE-encoded shikimate dehydrogenase was cloned, expressed and purified. Sequence alignment analysis shows that shikimate dehydrogenase of Mycobacterium tuberculosis exhibit the pattern of G-X-(N/S)-V-(T/S)-X-PX-K, which is highly conserved within the shikimate dehydrogenase family. The recombinant shikimate dehydrogenase spectrum determined by CD spectroscopy showed that the percentages for $\alpha$-helix, $\beta$-sheet, $\beta$-turn, and random coil were 29.2%, 9.3%, 32.7%, and 28.8%, respectively. The enzymatic characterization demonstrates that it appears to be fully active at pH from 9.0 to 12, and temperature $63^{\circ}C$. The apparent Michaelis constant for shikimic acid and $NADP^+$ were calculated to be about $29.5\;{\mu}M$ and $63\;{\mu}M$. The recombinant shikimate dehydrogenase catalyzes the substrate in the presence of $NADP^+$ with an enzyme turnover number of $399\;s^{-1}$. Zymological studies suggest that the cloned shikimate dehydrogenase from M. tuberculosis has a pretty activity, and the work should help in the discovery of enzyme inhibitors and further of possible antimicrobial agents against Mycobacterium tuberculosis.

• Plant Disease and Agriculture
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• v.5 no.1
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• pp.27-33
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• 1999

Frost injury of crops is closely related to the epiphytic population dynamics of ice nucleation-active (INA) bacteria, and the injury can be reduced by decreasing the INA bacterial population. In order to predict the epiphytic population of INA bacteria on crops, a rapid and accurate detection method has to be developed. In the previous report, we produced some antibodies against INA proteins purified from the outer membrane of INA bacteria. However it was difficult to produce the antibodies because the purification procedures of the INA proteins were complicated, and the final yield was too low. We designed a specific peptide from the N-terminal region of INA protein by computer analysis and synthesized the peptide in vitro in this experiment. The peptide sequence was Asp-Ser-Por-Leu-Ser-Leu-His-Ala-Asp, that is corresponding to the highly conserved region in several INA proteins, with predicted beta turn, coiling, and hydrophilic region. A polyclonal anti-INA peptide antiserum produced specifically recognized INA bacteria as few as 10 colony-forming units (CFU) in the ELISA reactions and did not respond to other non-INA bacteria. Serological specificity of the anti-INA peptide antiserum will facilitate the forecasting of the INA bacterial population dynamics on crops.