• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2004.10a
• /
• pp.163-163
• /
• 2004

• Proceedings of the Turfgrass Society of Korea Conference
• /
• 2005.04a
• /
• pp.26-27
• /
• 2005

• Proceedings of the Turfgrass Society of Korea Conference
• /
• 2005.04a
• /
• pp.28-29
• /
• 2005

• Asian Journal of Turfgrass Science
• /
• v.19 no.2
• /
• pp.191-193
• /
• 2005

• Asian Journal of Turfgrass Science
• /
• v.19 no.2
• /
• pp.177-183
• /
• 2005

• Proceedings of the Turfgrass Society of Korea Conference
• /
• 2002.02a
• /
• pp.6-9
• /
• 2002

• Weed & Turfgrass Science
• /
• v.6 no.4
• /
• pp.322-332
• /
• 2017

Microbial fertilizer has been used to prompt turfgrass growth and quality and to prevent turfgrass diseases in turfgrass management of golf courses. This study was conducted to evaluate effects of microbial fertilizer containing Lactobacillus fermentum (MFcL) on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), control fertilizer (CF), MFcL treatments [CF+$1.0g\;m^{-2}$(MFL), CF+$2.0g\;m^{-2}$ (2MFL)], and only MFcL treatment (OMF; $1.0g\;m^{-2}$ MFL). Chemical properties of soil by application of MFcL was unaffected. Turf color index, chlorophyll index, clipping yield and nutrient content and uptake of MFcL treatments were similar to CF. Furthermore, turfgrass shoot density of MFL was increased by 20% than that of CF, and that of OMF by 22% than NF. These results show that the application of microbial fertilizer containing L. fermentum increased turfgrass shoot density, which is not attributed to nutrient uptake in this study, but needs to be further investigated with other mechanisms such as biostimulant induction or phytohormone production.

• Asian Journal of Turfgrass Science
• /
• v.5 no.1
• /
• pp.11-22
• /
• 1991

This experiment was executed to investigate the possibility of the application of taxonomic method through the isoelectric focusing with polyacrylamide gel and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with seeds in the identification of turfgrasses. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to investigate the pattern of seed proteins which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The isoelectric focusing with polyacrylarnide gel was used to investigate the activity of the three isozymes of esterase, peroxidase and phosphoglucose isomerase which were extracted from 18 cultivars of cool season turfgrass and 4 cultivars of warm season turfgrass. The results were summarized as follows. 1. The difference of the patterns of seed proteins was observed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The identification of intra-genus was easily detected. 2. The three isozymes of esterase, peroxidase and phosphoglucose isomerase were investigated through isoelectric focusing with polyacrylamide gel. As a result, esterase was most effective among three isozymes in the identification of turfgrass cultivars 3. In the past cultivar identification was primarily based on visual morphological characters, but there was a lot of difficulty. If we should use electrophoresis, we will be able to identifvturfgrass cultivars more effectively.

• Asian Journal of Turfgrass Science
• /
• v.11 no.3
• /
• pp.161-166
• /
• 1997

This study was performed to develope the method of wildgrass to process as hydroseeding material which is a most popular revegetation method for Slope areas through the control of soil eroison and stability by seeding grasses. The wildgrass material for the processing were harvested at Suwon, Kyungki-do and Wonju, Kangwon-do in 1994. The investigations were established in laboratory for pot and field experiment with seven treatments by harvesting date and place in 1995. Results indicated that wildgrass harvested field in open space or road side was able to be used as hydroseeding material by proper processing. It was possible to substitute wildgrass seed to conrrnercial turfgrass seed, and plant tissue to mulching fiber by drying and crushing process. Germination of wildgrass seed was relatively slower than control (20g /$m^2$ turfgrass seed) in early stage of 20 days, but surface revegetation rate was higher in 50 days at wildgrass plot than control applied by normal hydroseeding method. Mixture of 5g /$m^2$ of cool season turfgrass seed to wildgrass material increased the rate of early stage of germination and surface revegetation. The application of wildgrass as hydroseeding material showed more naturalistic scenery than rutine hydroseeding with imported cool season turfgrass seed. Key words: Rydroseeding, Turfgrass, Wildgrass, Revegetation.

• Plant Biotechnology Reports
• /
• v.5 no.1
• /
• pp.61-69
• /
• 2011

The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.