• Plant Resources
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• v.8 no.3
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• pp.275-280
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• 2005

Tissue culture systems to optimize regeneration plant species of Ocimum spp were evaluated as a method to micropropagate individual plants and to better study their biology in vitro. Ocimum species were also evaluated for the production of natural plant products during and following the regeneration process. The primary goal of this project was to enhance the regeneration efficiency of basil. Several factors were examined using different Ocimum species and commercial varieties. The effect of cytokinin combination, activated charcoal, gelling agents, and different carbon sources were investigated. Anthocyanin callus spots were produced only in four varieties among six tested. 'Sweet Dani' showed the best results on anthocyanin accumulation, while 'African beauty', 'Tree basil' and 'Methylcinnamate' produced only a few spots. Shoot regeneration was only achieved from 'Sweet Dani' explants. As the activated charcoal concentration increased, callus formation rate decreased respectively compare to the controls for all varieties. There was a decrease in callus growth with increasing concentration of agar and phytagel.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.463-471
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• 2021

As a tropical plant, the neem tree (Azadirachta indica) has been used for a long time for disease and pest control and medical purposes. In this paper, we reviewed for the active ingredient of neem, the mode of action of azadirachtin in terms of insect growth regulation, repellent, feeding inhibition and oviposition against plant pests. And also we reviewed the current status of practical use in Korea. Among 57 products of neem-based eco-friendly organic agricultural materials distributed in Korea, seven products were certified for their efficacy. The average azadirachtin content of the seven products was 0.38%, which was 5.5 times less than the average content of 2.1% distributed worldwide. In the control effect on neem products in Korea, it showed some variation on aphids, but most showed a control effect of more than 90%. The treatment effects of Lycorma delicatula nymphs were 73-77%, and the control effects for thrips were obtained in the range of 50-72% mortality. The mortality effects against bug and moth species were generally low. It is expected that this review would provide important information necessary for the understanding of distributed neem products and the interpretation of experimental data.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1368-1374
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• 2005

To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 bp and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Taq I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.

• Agribusiness and Information Management
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• v.5 no.2
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• pp.27-37
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• 2013

Referral marketing plays an important role in promoting new products. When it comes to innovative agricultural products, early adopter's review or recommendation has a more critical impact on follower's purchase decision making. Hence, understanding of consumer's characteristics and needs play more important role in success of innovation. More particularly, other researchers pay attention to the role of consumer innovativeness. This study attempts to fill this gap in knowledge between innovative propensity of consumer and her/his intention to generate positive word of mouth about new agricultural products. Furthermore, in this paper, we adopt Vandecasteele and Geunes' motivated consumer innovativeness model to investigate consumer innovativeness in extrinsic motive and intrinsic motive level, and examine the moderating role of referral efficacy. For empirical verification, survey method is used for data collection. Partial least square (PLS) is adopted to analyze the data. Finally, several theoretical contributions and practical implications are discussed.

• The Plant Pathology Journal
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• v.20 no.2
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• pp.147-154
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• 2004

A simple and reliable procedure for RT-PCR detection of Apple stem pitting virus (ASPV), Cherry rasp leaf virus (CRLV), and Cherry necrotic rusty mottle virus (CNRMV) was developed. Two virus specific primer sets for each virus were found to specifically detect each virus among fourteen sets of designed oligonucleotide primers. Total RNAs extracted from healthy and from ASPV-,CRLV- and CNRMV-infected plant tissues were used to synthesize cDNA using oligo dT primer and then amplified by virus-specific primers for each virus. Each primer specifically amplified DNA fragments of 578 bp and 306 bp products for ASPV (prAS CP-C and prAS CP-N primers, respectively); 697 bp and 429 bp products for CRLV (prCR4 and prCR5-JQ3D3 primers, respectively); and 370 bp and 257 bp products for CNRMV (prCN4 and prCN6-NEG 1 primers, respec-tively) by RT-PCR. DNA sequencing of amplified DNA fragments confirmed the nature of each amplified DNA. Altogether, these results suggest that these virus specific primer sets can specifically amplify viral sequences in infected tissues and thus indicate that they can be used for specific detection of each virus.

• Journal of Applied Reliability
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• v.16 no.1
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• pp.48-55
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• 2016

Purpose: The purpose of this study is to identify the object-oriented metrics which have strong impact on the reliability and fault-proneness of software products. The reliability and fault-proneness of software product is closely related to the design properties of class diagrams such as coupling between objects and depth of inheritance tree. Methods: This study has empirically validated the object-oriented metrics to determine which metrics are the best to predict fault-proneness. We have tested the metrics using logistic regressions and artificial neural networks. The results are then compared and validated by ROC curves. Results: The artificial neural network models show better results in sensitivity, specificity and correctness than logistic regression models. Among object-oriented metrics, several metrics can estimate the fault-proneness better. The metrics are CBO (coupling between objects), DIT (depth of inheritance), LCOM (lack of cohesive methods), RFC (response for class). In addition to the object-oriented metrics, LOC (lines of code) metric has also proven to be a good factor for determining fault-proneness of software products. Conclusion: In order to develop fault-free and reliable software products on time and within budget, assuring quality of initial phases of software development processes is crucial. Since object-oriented metrics can be measured in the early phases, it is important to make sure the key metrics of software design as good as possible.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.6
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• pp.311-319
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• 2015

In this paper, in order to increase the production efficiency of the industrial plant, and predicts the resources of the manufacturing process, we have proposed a decision-making system for resource implementing the risk management effectively forecasting and risk management. A variety of information that occurs at each step efficiently difficult the creation of detailed process steps in the scenario you want to manage, is a frequent condition change of manufacturing facilities for the production of various products even within the same process. The data that is not contiguous products production cycle also not constant occurs, there is a problem that needs to check the variation in the small amount of data. In order to solve these problems, data centralized manufacturing processes, process resource prediction, risk prediction, through a process current status monitoring, must allow action immediately when a problem occurs. In this paper, the range of change in the design drawing, resource prediction, a process completion date using a regression algorithm to derive the formula, classification tree technique was proposed decision system in three stages through the boundary value analysis.

• Journal of Forest and Environmental Science
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• v.32 no.3
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• pp.302-310
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• 2016

The loss and degradation in tropical forest region are some of the current global concern. Hence, these issues elevated the role of rehabilitated forests in providing ecological products and services. The information on the carbon stock is important in relation to global carbon and biomass use, but lacking from the tropical region. This paper reports the assessment of tree and soil carbon stock in a chronosequence rehabilitated tropical forest stands in Malaysia. The study site was at the UPM-Mitsubishi Forest Rehabilitation Project, UPMKB. $20{\times}20m$ plot was established each and assessed in 2009 at 1-, 10- and 19-year-old sites while an adjacent ${\pm}23-year-old$ natural regenerating secondary forest plot was established for comparison. The overall total carbon stock was in the order of 19-year-old>${\pm}23-year-old$>10-year-old>1-year-old. When forest carbon stock is low, the soil component plays an important role in the carbon storage. The forest carbon recovery is crucial to increase soil carbon stock. The variations in the carbon stock showed the different stages of the forest recovery. Species survived after 19-years of planting are potential species for carbon sequestration activities in rehabilitated forest. Human intervention in rehabilitating degraded forest areas through tree planting initiatives is crucial towards recovering the forest ecological role especially in forest carbon stock capacity.

• Journal of the Korean Wood Science and Technology
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• v.46 no.1
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• pp.100-106
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• 2018

This study was fulfilled to investigate the anatomical characteristics according to provinces. In order to compare anatomical characteristics between provinces we selected Goseong, Hongcheon, and Bonghwa as experimental sites. To use the tree rings formed at the same years we dated all tree rings by cross-dating method used in dendrochronology and the cutting years were successfully dated in 2014, 2012, and 2014 for woods from Goseong, Hongchen, and Bonghwa, respectively. Based on the cutting years tracheid lengths and widths were measured considering juvenile wood (tree rings between the pith and first 10 years), heartwood (tree rings formed between 1955 and 1964), and sapwood (tree rings formed between 2002 and 2011). According to the results about differences between tracheid lengths from three provinces, juvenile wood did not show a difference, statistically, but heartwood and sapwood showed differences as the tracheid lengths following Bonghwa, Hongcheon, and Goseong. Bongwha also showed the largest value in the trachied width among three provinces. The tracheid lengths in the radial direction increased up to around the first 20 years, and then they showed stabilized. All wood samples showed typical Korean red pine's characteristics in anatomical observation under a light microscope.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.422-428
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• 1998

Polymerase chain reaction(PCR)-based inter-simple sequence repeats(I-SSR) markers were analyzed in 48 megagametophytes of a single tree of Abies koreana $W_{ILS}$. Nineteen of the 35 primers, screened with 6 megagametophyte DNA and produced the clearest amplification products in the preliminary experiment, were used for PCR with 48 megagametophyte DNAs sampled from a single tree. On the basis of the chi-square test, a total of 51 amplicons, amplified by the 19 primers, were revealed to be segregated according to the Mendelian ratio(i.e., 1 : 1 segregation ratio) in the 48 megagametophytes at 5% significance level. Based on the linkage analysis, the observed 51 Mendelian loci turned out to be unlinked each other, which suggested that they are evenly distributed in the genome. However, majority of RAPD markers are known to belong to the independent linkage blocks, which frequently results in the amplification of RAPD markers from the restricted regions of the genome. Owing to the nature of even distribution of the 51 loci observed in this study, the I-SSR markers could give better resolution of estimating genetic diversity from the whole genome than RAPD markers. And I-SSR markers are also more suitable than RAPD markers for reconstructing phylogenetic relationship by a cladistic method which requires to fulfil the assumption of independent evolution of the different characters.