• The Korean Journal of Nuclear Medicine Technology
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• v.24 no.1
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• pp.33-38
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• 2020

Purpose Vitamin D is essential for maintaining bone health, controling cell proliferation or differentiation, strengthening immune function by controlling calcium metabolism in the body. Vitamin D deficiency can lead to increase the risk of rickets, osteoporosis, cardiovascular disease, diabetes and cancer. Especially, South Korea is one of the highest population proportion of vitamin D deficiency. Accurate determination of levels of 25-OH-VitD or 25-OH-VitD3 in blood serum is required for the diagnosis and treatment of vitamin D deficiency. In this study, radioimmunoassay of 25-OH-VitD and 25-OH-VitD3 was performed and compared to evaluate the effectiveness of Vitamin D radioimmunoassay. Materials and Methods Serum 25-OH-VitD and 25-OH-VitD3 levels were measured using radioimmunoassay. The interrelationship, reproducibility and population distribution rate were evaluated. In addition, the internal quality control was performed at Asan Medical Center from April 2017 to June 2019 and the result of external quality control (Interagency proficiency evaluation) of first and second half of 2018 hosted by the Korean Society of Nuclear Medicine Technology (KSNMT). Both tests were measured by same manufacturer's reagent. Results 25-OH-VitD showed a strong positive correlation on 97 samples, as 25-OH-VitD3 x 0.9 + 0.3 (R>0.9). In repeated measurement, the average Diff(%) value of the reproducibility evaluation of 25-OH-VitD and 25-OH-VitD3 were 7.7% and 7.4%, respectively. Population distribution results showed no statistically significant differences(p>0.05). The resultant value of internal quality control, which measured from April, 2017 to June 2019 in Blood test room of Nuclear Medicine at Asan Medical Center, showed average (CV%) 6.2% and 6.8%, respectively. As a result of the external quality control (interagency proficiency evaluation) Z value obtained under 2.0, as shown below; Conclusion The interrelationship, reproducibility, population distribution rate, internal quality control and external quality control between 25-OH-VitD and 25-OH-VitD3 radioimmunoassay shows superior outcome. Radioimmunoassay, which can be alone measured in the blood as 25-OH-VitD or 25-OH-VitD3, is considered suitable screening tests for the diagnosis of vitamin D deficiency.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.583-593
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• 2015

This study was conducted to investigate the effects of mixed-sowing of legume forage and applying of cattle manure on the productivity of organic rye to provide dairy farmers with safe organic feeds. Also the present study aimed to evaluate optimal applying level of cattle manure and carrying capacity of Korean native cattle (Hanwoo) per unit area. The amount of crude protein was significantly higher in the mixed-sowing of legume forage (average 760~786 kg/ha) than in the single-sowing of rye (average 353 kg/ha) (p<0.05). The mixed-sowing of forage peas and the single-sowing of rye tended to improve by increasing the level of nitrous fertilization rather than the fertilization treatment. The amount of crude protein on the rye according to the mixed-sowing of legume was significantly higher in the mixture (average 8.29~9.90%) than in the single (average 4.93%) (p<0.05). The comparison by the level of nitrogen fertilization indicated significantly high for the rye in 50 kg N/ha than in fertilization treatments (p<0.05). Total digestible nutrients (TDN) amount in the single-sowing of rye was average 46.86% and has lower than average 49.96~50.12% of the mixed-sowing of legume, and especially the mixed-sowing of forage pea was the highest with 54.55% in 150 kg N/ha for the level of nitrogen fertilization (p<0.05). The breeding ability of annual organic livestocks per unit area according to the feed value of rye presented significantly higher ability in the mixed-sowing of legume (3.72~4.12 heads) than the single-sowing of rye (average 2.26 heads) (p<0.05). By summarizing above results, the mixed-sowing of legume is required to improve the productivity and the feed value of rye for increasing organic livestock breeding ability in southern area of Gyeongbuk regions, and the study for identifying the appropriate fertilization level using livestock excretions is further necessary.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.456-461
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• 2010

A study was conducted to investigate the effect of topophysis, and uniting method of rootstock and scion on rooting and subsequent growth of stenting-propagated cut rose ($Rosa$ $hybrida$ Hort.) in an effort to develop an efficient stenting propagation method for domestic rose cultivars. Four cultivars used in this study were two standard type cultivars 'Sweet Yellow' and 'Hanmaum', and two spray type cultivars 'Chelsi' and 'May'. Scions were grafted on cuttings of a rootstock $Rosa$ $indica$ 'Major'. The stenting-propagated scion-rootstock unions were planted in rockwool cubes ($50{\times}50{\times}50mm$, Delta, Grodan, Denmark) and were placed in a graft-take chamber for five days before being placed on misted greenhouse beds. The rootstock was removed of all leaves and nodes. Both the base of scions and top of stocks were simultaneously cut at a $45^{\circ}$ angle for grafting. Scions were prepared as single node cuttings, each with a five-leaflet leaf. Three positions of topophysis used were 7-9th (top), 4-6th (middle), and 1st-3rd (bottom) nodes from the stem base. Four uniting materials used were tube, tube + parafilm wrap, tube + clothespin, and clothespin. Rooting and growth were affected by the topophysis and cultivar. The best topophysis for rooting was 7-9th (top) nodes in all cultivars. Topophysis affected percent rooting, and number of roots, length of the longest root, and but not weight, shoot length and graft-take. Rooting and growth were affected by the uniting method and cultivar. Tube uniting method generally showed higher percentage graft-take, percent rooting, and number of roots than other methods. However, rootstock and scion union was not complete in this treatment. On the whole, the greatest rooting and subsequent growth of stenting-propagated plants were found in the tube + clothespin method. Except 'Sweet Yellow', rooting and growth were not adequate in the clothespin method. The results suggested that a tube + clothespin method was the most effective, and this method may be used as a substitute to save labor compared to a tube + parafilm wrap method which is currently being used in commercial nurseries.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.523-530
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• 2013

This study investigated the influence of seasonal incidence and defoliation degree caused by Marssonina blotch (Diplocarpon mali Harada et Sawamura) on shoot growth and fruit quality of 'Fuji'/M.9 apple tree. The occurrence of marssonina blotch in Gunwi region was observed from the mid of July 2009, and percentage of defoliation was about 10% in the end of October. In 2010, that started in the early of June. By the end of September, percentage of defoliation was 20% and it reached 50% in late October. Secondary growth of terminal shoot in 2010 was three times as much as that in 2009. In 2009, the soluble solid contents and the red color (hunter a value) during fruit maturation increased to $13.8^{\circ}Brix$ and 16.2, respectively. In 2010, the soluble solid content during fruit maturation remained in the $12.1-12.6^{\circ}Brix$ range after early October, and the red color began to increase after the end of September when the percentage of defoliation was 20%. As for fruit quality by defoliation degree, the red color decreased when defoliation percentage of bourse shoot at the end of October was more than 30%. Fruit weight and soluble solids also decreased when defoliation percentage was more than 50%. Fruit length and diameter of over 30% defoliation treatments begun to decrease after the middle of August, compared with those of under 30% defoliation treatment. Photosynthetic rate of the leaves that was located at the secondary growth of bourse shoot was similar to that of leaves which was located at the middle of bourse shoot. However, the size and the soluble solid content of fruit were not affected by photosynthesis activities of bourse shoot leaves which was develop in the secondary shoot growth.

• Korean Journal of Ecology and Environment
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• v.35 no.4 s.100
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• pp.273-284
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• 2002

During January 1998-October 1999, the impact of free-floating plants (FFP) on limnology of the wetland ecosystem was evaluated through the investigation of physicochemical characteristics of the Woopo Wetland along with in situ manipulation experiments of aquatic plants. Flooding events occurred in the wetland during the summer period (Jun.-Aug.) and water levels rose to 2-3 m due to precipitation from the catchment and inflow from the main channel of the Nakdong River. Physicochemical parameters and plankton dynamics in the wetland during the summer were greatly influenced by floods and growth of free-floating plants. Dissolved oxygen (Jun.-Sept., 4.5${\pm}$2.5 mg/1; Oct.-May, 8,1 ${\pm}$4.0 mg/1) and pH (Jun.-Sept., 6.9${\pm}$0.4; Oct.-May,7.4${\pm}$0.8) levels were significantly lower during the summer than any other seasons. Three types of enclosure experiments (100 L, treatments with floating plants, screened and opened) were conducted under the presence and absence of sediment for 15 days in the 1999 summer. The treatments with sediment had higher levels of nutrient concentrations than those of the others. Among the treatments with sediment, nutrient concentrations in the treatments with free-floating plants were higher than the others. Zooplankton communities in each treatment showed a similar variation, although the scale of zooplankton densities differed. Rotifer community dominated the zooplankton at the initial phase of the experiment, but decreased drastically along with an increase of cladoceran and copepod communities. In conclusion, low levels of dissolved oxygen and pH in the Woopo Wetland during the summer seemed to be caused by a proliferation of free-floating plants and active decomposition process at the bottom of the sediment.

• Journal of Animal Environmental Science
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• v.11 no.1
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• pp.45-54
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• 2005

A field survey was conducted to determine the concentration of odor compounds from pig buildings and that were 20 meters within the boundary area. The odor compounds were measured from large, medium and small farms with enclosed and open housing systems and slurry and sawdust manure fermentation treatment methods. Among the odor compounds investigated, ammonia ($NH_3$) had the highest concentration at 0.9 ${\~}$ 21.0 ppm followed by Hydrogen Sulfide($H_2S$) with a wide variation concentration of 51.9 ${\~}$ 6,712.4 pub, Uethylmercaptan($CH_3SH$) with non-detectable (N. D.) ${\~}$ 12.9 ppb, Dimethylsulphide($(CH_3)_2S$), with N. D. ${\~}$ 5.2 ppb and Dimethyldisulphide($(CH_3)_2S_2$) with N. D. ${\~}$ 2.6 ppb. Considering the prevailing wind direction and air velocity ranging from 0.23 to 0.73 m/s within the boundary area, the odorous matters; $NH_3$, $H_2S$, $CH_3SH$, $(CH_3)_2S_2$ and $(CH_3)_2S$ were 0.2${\~}$4.5 ppm, 0.01 ${\~}$0.06 ppb, N. D. ${\~}$0.009ppb, N. D.${\~}$0.002ppb and N. D. for $(CH_3)_2S$ respectively. These findings suggested that the Odor compounds $(CH_3)_2S_2$ had the lower detection in the boundary area whilst $(CH_3)_2S$ had no detection level within a 20-meter distance only. However, with these results odor compounds from pig buildings has to be further investigated under more controlled environmental factors.

• Journal of Animal Environmental Science
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• v.18 no.sup
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• pp.21-28
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• 2012

Since the temperature during summer is higher than the temperature which is suitable for milking cows, high temperature stress have severely affected dairy farmers due to decreased milk production. This study was carried out to investigate the effect of rainwater sprinkling system for reducing heat stress in milking cows during intense heat time. Treatment conditions were divided into 2 types ; No rainwater sprinkling (T1) and Rainwater sprinkling (T2). Temperature in the milking cow shed, feed intake, milk yield, respiration rate and rectal temperature were measured to investigate the effect of hot environment on the physiological, productive responses in milkig cows. The results obtained are summarized as follows : The daily milk yields were 24.5 and 27.4 kg per head in T1 and T2 during experimental period. The respiration rate was significantly higher in T1 than those of T2. The rectal temperature in T1 ($40.6^{\circ}C$) was higher than those of T2 ($39.8^{\circ}C$). It was suggested that rainwater sprinkling system installed in the dairy cow shed was effective means to reduce heat stress of milking cow.

• Journal of Animal Environmental Science
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• v.16 no.1
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• pp.41-50
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• 2010

The goal of this study was to develop a suitable ventilation system for high-rise hog building (HRHB) for growing-fattening with combined slatted floor pen in second story and in situ manure management system in Korea. The HRHB was constructed as 29m long, 9m wide and 7.6m high for outer dimension with an indoor height of 3.1m and 2.4 for lower and upper floor, respectively. Ventilation systems for each treatment were installed in separated rooms of HRHB. The ventilation types installed in each room were following 3 types: ventilation type 1 (V1), where air was pulled through a circular duct inlet and exhausted by fans; ventilation type 2 (V2), where air was pulled through eave inlet (side ceiling inlet) and exhausted by fans; and ventilation type 3 (V3), where air was pulled through baffled ceiling inlet and exhausted by fans. For each ventilation system, investigated air velocity under minimum, medium and maximum ventilation ratio and air flow pattern inside. The results were as follows; For air flow pattern from top to bottom, V1 showed a homogeneous vertical type, V2 showed a bilateral symmetry type and V3 showed an vertical umbrella type. Under minimum ventilation ratio, air velocity in upper floor (80cm above the slated floor) was similar for V1, V2, and V3. Under maximum ventilation ratio, air velocity in upper floor was undeviating for V1 (0.10~0.26m/s) and varied for V2 (0.12~0.63m/s) while those for V3 was relatively slow and less varied (0.07~0.15m/s). In conclusion, Duct inlet type (V1) can be applied to the development of a new HRHB with additional evaluations such as field test hog feeding.

• Journal of Life Science
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• v.12 no.1
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• pp.16-25
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• 2002

To summarize our interpretation of the results, we can explain shown below. Optimum con dictions in order to soften of sea tangle leafs were treated in the solutions of 0.05% $CH_3$COOH at 9$0^{\circ}C$ for 0.5 hour, 0.2% $K_2$HPO$_4$at 10$0^{\circ}C$ for 0.5 hour and 0.3% NaHCO$_3$at 10$0^{\circ}C$ for 0.5 hour. After sea tangle leafs were treated in the solutions of 0.05% $CH_3$COOH at 9$0^{\circ}C$ for 0.5 hour and added 10% seasoning agent of 0.5% glutamic acid, 3% glycine, 5% sorbitol and 1.5% soy sauce. Contents of free amino acid in the leaflike tea were a large amount as alanine of 707.2 $\mu$mo1/100$m\ell$ and glutamic acid of 343.6 $\mu$mo1/100 $m\ell$. And contents of mineral were order Na of 49.38 ppm, Mg of 10.72 ppm, K of 10.56 ppm and Ca of 6.55 ppm. Powder tea was added 0.05% glutamic acid, 5% glycine, 5% glucose and 4% sodium chloride in sea tangle powder, and then pressure treatment at 11$0^{\circ}C$ for 1.5 hours. Contents of free amino acid in the powder tea were a large amount as glycine of 222.04 $\mu$mo1/100$m\ell$ and glutamic arid of 208.58 $\mu$mol/100$m\ell$. And contents of mineral were order Na of 104.24 ppm, Mg of 14.31 ppm, K of 9.68 ppm, Fe of 2.36 ppm, Ca of 2.00 ppm, Zn of 0.13 ppm, Cu of 0.10 ppm and Mn of 0.01 ppm.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.4
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• pp.247-254
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• 2003

A study was carried to compare two varieties of tall fescue(Festuca arundinacea Schr.): Fawn (endophyte infection) and Roa(endophyte-free). The study examined plant height, botanical composition, and dry matter yield of mixed pasture. The cattle grazed on a mixed pasture of tall fescue species and orchardgrass(Dactylis glomerata L.), perennial ryegrass(Lolium perenne L.) and white clover(Trifolium repens L.) during March 22, 1997, to September 21, 1997. The number of grazing animals was 4.3/ paddock(50m x50m) and was adjusted according to the condition of pasture. Dry matter yields determined on ungrazed pasture(7-time harvests) were 1,690 $\pm$407kg and 1,128 $\pm$ 238kg/ha on pastures consisting of Fawn and Roa, respectively. This difference was significant(P<0.01). There were 17.71 $\pm$ 1.27cm and 12.83$\pm$0.90cm in average plant lengths of Fawn and Roa(P<0.01), respectively The botanical composition of tall fescue and orchardgrass in the mixture treatment included in the Fawn variety were shown in 34.2 and 3.8％, comparing Roa with 17.1 and 9.6％, on the 21th of September, 1997, respectively. Tall fescue Roa represented a higher botanical composition than orchardgrass(a main species on Cheju). Crude protein content of Fawn was slightly less than that of Roa. From these results, we may conclude that Roa variety is nutritionally better, but is less adaptible to high temperature climate than Fawn. However, Roa variety is still better than orchardgrass in high temperature conditions.