• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.138-138
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• 2017

Agriculture is the most primitive civilized Activities of mankind but also the propellant of civilization development. Because it is the most basic material goods source of mankind. Among these materials rice is one of the most important part of these, we call them the substance of survival. From the beginning of the agricultural activities to the present we have experienced three industrial revolutions and are experiencing the Fourth Industrial Revolution. With the development of science and technology makes the efficiency of agricultural production is higher and higher, but compared with the original we are facing the same problem: natural disasters; pests and diseases; now also face the depletion of resources, environmental degradation and other issues. Therefore, improve and cultivate new crop varieties to make it better resistance and more production for better develop modern agriculture. It's very helpful for human social development. And also it is the responsibility and task of modern molecular breeding. In this study, I used bacterial leaf blight to find a better resistance gene to improve the resistance of rice. Frist Cultivate k3 of bacterial leaf blight, than inoculation by leaf clipping method (Kauffman,1973) in CNDH and SNDH population at 40days after rice transplanting. Check the lesion length by inoculation plants at 14days after inoculation, and record data for QTL analysis program. Than I get 4 intervals in 3 different chromosomal regions. I found these defense genes in the 4 intervals. So I used NCBI Justbio, Rapdb, etc. to finding these genes in physical map, than design primer for map base cloning. At last these defense genes will be employed in further research for introduction of the gene to the parental plant and rice breeding for solving food crisis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.3
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• pp.259-266
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• 2011

To produce cupped oyster sprat consistently, we investigated the efficiency of nine settlement substrates using selective breeding of Pacific oyster Crassostrea gigas, with the goal of popularizing off-bottom culture on the west coast of Korea. We also compared the growth and survival of selectively bred sprat (diploid) with triploid sprat from an off-bottom culture system. Considering, the attachment rate and detachment efficiency, producing cupped oyster in a polypropylene gunny bag proved to be the most effective method. There were no differences in shell growth or total weight between the diploids from selective breeding and triploids from off-bottom culture for 5 months. However, the survival rate was 8 times higher in the diploids than the triploids. Transplanting sprat from selective breeding is one way to restore oyster farms and nurture off-bottom culture along the west coast.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.5
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• pp.533-542
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• 2011

Numerous seagrass habitat restoration projects have been attempted recently due to the remarkable decline in seagrass coverage. Seagrass transplants tend to adapt to a new environment after experiencing transplanting stress during the early stages of transplantation. Once acclimated, the transplants grow into healthy seagrass beds via vegetative propagation. The establishment and growth dynamics of transplanted seagrasses in bays and coasts are widely reported, but few studies have been conducted on estuaries in Korea. We transplanted Zostera marina in November 2007 and November 2008 in the Nakdong estuary using the staple method, and monitored the survival, adaptation, and growth dynamics of the transplants as well as environmental factors every month for 1 year. Both transplants adapted well to the new environment without initial losses and showed rapid productivity during early summer. However, density of transplants increased 320% in 1 year from the previous year's transplants but that decreased to 59% during the following year. This significant reduction in density in the second year may have been caused by exposure to low salinity (10 psu) for 3 weeks during the unusually long monsoon season. While the survival and growth dynamics of seagrass transplants planted in bays and coasts are mainly controlled by underwater photon flux density and water temperature, salinity was the critical factor for those planted in Nakdong estuary.

• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.105-111
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• 2005

End-stage renal disease is a fatal and devastating disease that is caused by progressive and irreversible loss of functioning nephrons in the kidney. Dialysis and renal transplantation are the common treatments at present, but these treatments have severe limitations. The present study investigated the possibility of reconstructing renal tissues by transplantation of renal precursor cells to replace the current treatments for end-stage renal disease. Embryonic renal precursor cells, freshly isolated from metanephroi of rat fetus at day 15 post-gestation, were seeded on biodegradable polymer scaffolds and transplanted into peritoneal cavities of athymic mice for three weeks. Histologic sections stained with hematoxylin & eosin and periodic acid-Schiff revealed the formation of primitive glomeruli, tubules, and blood vessels, suggesting the potential of embryonic renal precursor cells to reconstitute renal tissues. Immunohistochemical staining for proliferating cell nuclear antigen, a marker of proliferating cells, showed intensive nuclear expression in the regenerated renal structures, suggesting renal tissue reconstitution by transplanted embryonic renal precursor cells. This study demonstrates the reconstitution of renal tissue in vivo by transplanting renal precursor cells with biodegradable polymer scaffolds, which could be utilized as a new method for partial or full restoration of renal structure and function in the treatment of end-stage renal disease.

• Mycobiology
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• v.29 no.4
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• pp.218-223
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• 2001

A rapid radicle assay for prescreening antagonistic bacteria to Phytophthora capsic4 causal agent of Phytophthora blight of pepper was developed. Sixty-four bacterial strains with in vitro antifungal activity selected out of 1,400 strains isolated from soils of Ansung, Chunan, Koyang, and Paju, Korea in 1998 were used for development of the bioassay. Uniformly germinated pepper seeds dipped in bacterial cells for 3 hours were placed near the edges of growing mycelia of P. capsici on water agar containing 0.02% glucose. Five-week-old pepper plants(cv. Nockwang) were inoculated to compare with results of the radicle assay developed in this study. For plant inoculation, pepper seeds were sown in potting mixtures incorporated with the bacterial strains, then transplanted into steam-sterilized soils 3 weeks later. Plants were hole-inoculated with zoospores of P. capsici 2 weeks after transplanting. Disease incidence and severity were determined in radicle and plant assessments, respectively. In radicle assay, six strains, GK-B15, GK-B25, OA-B26, OA-B36, PK-B09, and VK-B14 consistently showed the significant(P=0.05) disease reduction against radicle infection by the fungus, four of which also did in plant assessments. Strains OA-B36 and GK-B15 consistently reduced the fungal infection in both the radicle assay and the plant assessment. Therefore, prescreening strains using the radicle assay developed in this study followed by plant assay could reduce time and labor, and improved the possibility of selecting antagonistic bacteria for control of Phytophthora blight of peppers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.255-262
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• 1997

To estimate the total dry matter(TDM) of rice plant by non-destructive method, spectral reflectance from rice plant canopy was measured by using the spectroradiometer (LI-1800, LICOR Inc.) with one week interval during the rice growing season at Suwon paddy field in 1993. Two medium late maturing rices, Daechung-byeo Ilpum-byeo, and one early maturing variety, Jinbu-byeo, were cultured to observe TDM, then they were compared with those estimated by vegetation index together. Vegetation index determined by the reflectance of visible against near infrared wavelength showed high correlation with TDM. Vegetation index derived from narrow band(10nm interval) ratio, R910/R460, has the highest correlation coefficient with TDM. TDM estimated from R910/R460 from transplanting to heading stage corresponded well to measured values (Y=21.2428X-212.734 ; $R^2$=0.87). But another vegetation index, NIR(720~1,100nm) /Red(600~700nm) showed higher correlation with TDM than NIR(720~1,100nm) /Blue(400~500nm) did from heading stage to maturity.

• Korean Journal of Weed Science
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• v.9 no.3
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• pp.238-244
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• 1989

The experiment was carried out to evaluate the herbicidal properties of dithiopyr(3, 5-pyridine dicarbothioic acid-2-(difluoromethyl)-4-(2-methylpropyl)-6-(trifluoromethyl)-S,S-dimethyl ester). Weeding effect of dithiopyr was determined under various factors such as dosage, soil type, leaching amount, temperature, water depth, overflow time, and application time and method. Dithiopyr showed very high phytotoxic activity on most major annuals weeds, expecially Echinochloa crus-galli and Monochoria vaginalis. Excellent control of Echinochloa crus-galli was obtained at 0.04kg a.i./ha with preemergence application and 0.12kg a.i./ha with early post-emergence application (12 DAT ; Echinochloa crus-galli of 1.5 leaf stage). Weeding effect of dithiopyr decreased when applied at the soil surface 2days before transplanting. Soil type, leaching amount, temperature, water depth, overflow time did not affect the weeding effect.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.266-271
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• 2010

This study was conducted to improve weeding effect by rice bran farming technique. In the weeding effect experiment by application timing control of rice bran and soybean cake, weeding efficacy was the highest at the treatment of conventional application of rice bran. The treatment of soybean cake with rice bran was unfavorable, but Echinochloa crus-galli occurred little at the treatment of soybean cake at 5 days after transplanting, so it is thought that further study about inhibitor of early E. crus-galli occurrence in soybean cake is necessary. Cultivating weeder was effective at the treatments of rice bran and soybean cake together, of which weed occurrence was more than conventional rice bran, and precise experiments about cultivating weeding time and method will be necessary.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.3
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• pp.141-144
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• 2006

This experiment investigated seed yield and grain quality of rice plants treated with different irrigation methods (water supply until complete saturation, field capacity, and surface soil crack) compared with a conventional irrigation method (inundation). Each treatment began 20 days after transplanting and ended 35 days after heading. There was an 8, 18 and 18% reduction in irrigation water in the three treatments, respectively. Rice yield with complete saturation treatment was similar to that of conventional irrigation, while those of field capacity and soil crack were less by 7 and 13%. The ratio of filled grain was lower and amylose content was higher in the water-saving irrigation than those from conventional irrigation.

• Journal of Biosystems Engineering
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• v.38 no.4
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• pp.255-263
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• 2013

Purpose: It is not easy to drive a rice-transplanter avoiding underlapped or overlapped transplanting in paddy fields. An automated guidance system for the riding-type rice-transplanter would be necessary to operate the rice-transplanter autonomously or to assist the beginning drivers as a driving aid. Methods: A prototype of guidance system was composed of embedded computers, RTK-GPS, and a power-steering mechanism. Two Kalman filters were adopted to overcome sparse positioning data (1 Hz) from the RTK-GPS. A global Kalman filter estimated the posture of rice-transplanter every one second, and a local Kalman filter calculated the posture from every new estimation of the global Kalman filter with an interval of 200 ms. A PID controller was applied to the row-following mode control. A control method of U-turning mode was developed as well. A stepping motor with a reduction gear set was used to rotate the shaft of steering wheel. Results: Test trials for U-turning and row-following modes were done in a paddy field after some parameters have been tuned at the ground speed range of 0.3 ~ 1.2 m/s. The minimum RMS error of offset was 3.13 cm at the ground speed of 0.3 m/s while the maximum RMS error was 13.01 cm at 1.2 m/s. The offset RMS error tended to increase as the ground speed increased. The target point distance, LT also affected the system performance and PID controller parameters should be adjusted on different ground speeds. Conclusions: A target angle-based PID controller plus stationary steering angle controller made it possible for the rice-transplanter to steer autonomously by following a reference line accurately and even on U-turning mode. However, as condition in paddy fields is very complicated, the system should control the ground speed that prevents it from deviating too much due to ditch and slope.