• Structural Engineering and Mechanics
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• 제55권3호
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• pp.605-638
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• 2015

It is intended to perform buckling analysis of steel gabled frames with tapered members and flexible connections. The method is based on the exact solutions of the governing differential equations for stability of a gabled frame with I-section elements. Corresponding buckling load and subsequently effective length factor are obtained for practical use. For several popular frames, the influences of the shape factor, taper ratio, span ratio, flexibility of connections and elastic rotational and translational restraints on the critical load, and corresponding equivalent effective length coefficient are studied. Some of the outcomes are compared against available solutions, demonstrating the accuracy, efficiency and capabilities of the presented approach.

• 한국해양바이오학회지
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• 제1권1호
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• pp.34-39
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• 2006

Molecular biology and microalgal biotechnology have the potential to play a major role in improving the production efficiency of a vast variety of products including functional foods, industrial chemicals, compounds with therapeutic applications and bioremediation solutions from a virtually untapped source. Microalgae are a source of natural products and have been recently studied for biotechnological applications. Efficient genetic transformation systems in microalgae are necessary to enhance their potential to be used for human health. A microalga such as Chlarella is a eukaryotic organism sharing its metabolic pathways with higher plants. This microalga is capable of expressing, glycosylating, and correctly processing proteins which normally undergo post-translational modification. Moreover, it can be cultured inexpensively because it requires only limited amount of sunlight and carbon dioxide as energy sources. Because of these advantages, Chlarella may be of great potential interest in biotechnology as a good candidate for bioreactor in the production of pharmaceutical and industrial compounds for human functional foods. Here, we briefly discuss recent progress in microalgal transgenesis that has utilized molecular biology to produce functional proteins and bioactive compounds.

• Steel and Composite Structures
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• 제29권1호
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• pp.139-149
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• 2018

In this paper a new multi-step damage detection approach is provided. In the first step, condensed modal residual vector based indicator (CMRVBI) has been proposed to locate the suspected damaged elements of structures that have rotational degrees of freedom (DOFs). The CMRVBI is a new indicator that uses only translational DOFs of the structures to localize damaged elements. In the next step, salp swarm algorithm is applied to quantify damage severity of the suspected damaged elements. In order to assess the performance of the proposed approach, a numerical example including a three-layer square laminated composite plate is studied. The numerical results demonstrated that the proposed CMRVBI is effective for locating damage, regardless of the effect of noise. The efficiency of proposed approach is also compared during both steps. The results demonstrate that in noisy condition, the damage identification approach is capable for the studied structure.

• Structural Engineering and Mechanics
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• 제29권6호
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• pp.643-658
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• 2008

In this paper, we present a quadratic element model based on non-conforming displacement modes and modified shape functions. This new and refined 8-node hybrid stress plane element consists of two additional non-conforming modes that are added to the translational degree of freedom to improve the behavior of a membrane component. Further, the modification of the shape functions through quadratic polynomials in x-y coordinates enables retaining reasonable accuracy even when the element becomes considerably distorted. To establish its accuracy and efficiency, the element is compared with existing elements and - over a wide range of mesh distortions - it is demonstrated to be exceptionally accurate in predicting displacements and stresses.

• 약학회지
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• 제49권3호
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• pp.205-211
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• 2005

The 3' UTR (3' untranslated region) plays important roles in controlling gene expression through regulating 3' polyadenylation, mRNA export, subcellular localization, translational efficiency, and mRNA stability. Changes in the 3' UTR sequence in an expressed transcript can result in functional changes of the genes that are expressed in pathological conditions compared with those genes expressed in normal physiologic conditions. A genome-wide survey of 3' UTR variation was performed for the genes expressed during hematopoietic differentiation from CD34+ stem/progenitor cells to CD 15 + myeloid progenitor cells. Wide-spread differential usage of the 3' UTR was observed from the genes expressed during this cellular transition. This study implies that the 3' UTR can be a highly coordinated region for post-transcriptional regulation of the function of expressed genes.

• Steel and Composite Structures
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• 제33권3호
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• pp.403-432
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• 2019

Axially functionally graded (AFG) beams are a new class of composite structures that have continuous variations in material and/or geometrical parameters along the axial direction. In this study, the exact analytical solutions for the free vibration of AFG and uniform beams with general elastic supports are obtained by using Euler-Bernoulli beam theory. The elastic supports are modeled with linear rotational and lateral translational springs. Moreover, the material and/or geometrical properties of the AFG beams are assumed to vary continuously and together along the length of the beam according to the power-law forms. Accordingly, the accuracy, efficiency and capability of the proposed formulations are demonstrated by comparing the responses of the numerical examples with the available solutions. In the following, the effects of the elastic end restraints and AFG parameters, namely, gradient index and gradient coefficient, on the values of the first three natural frequencies of the AFG and uniform beams are investigated comprehensively. The analytical solutions are presented in tabular and graphical forms and can be used as the benchmark solutions. Furthermore, the results presented herein can be utilized for design of inhomogeneous beams with various supporting conditions.

• BMB Reports
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• 제52권5호
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• pp.324-329
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• 2019

Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols.

• 한국전기전자재료학회논문지
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• 제37권2호
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• pp.133-140
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• 2024

This study offers a comprehensive evaluation of the role and impact of advanced power semiconductors in solar module systems. Focusing on silicon carbide (SiC) and gallium nitride (GaN) materials, it highlights their superiority over traditional silicon in enhancing system efficiency and reliability. The research underscores the growing industry demand for high-performance semiconductors, driven by global sustainable energy goals. This shift is crucial for overcoming the limitations of conventional solar technology, paving the way for more efficient, economically viable, and environmentally sustainable solar energy solutions. The findings suggest significant potential for these advanced materials in shaping the future of solar power technology.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3757-3760
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• 2013

MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells. Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR-485 might play an important role in breast cancer by suppressing cell proliferation and migration.

• Reproductive and Developmental Biology
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• 제40권1호
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• pp.7-13
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• 2016

Effectiveness of transgene transfer into genome is crucially concerned in mass production of the bio-pharmaceuticals using genetically modified transgenic animals as a bioreactor. Recently, the mammary gland has been considered as a potential bioreactor for the mass production of the bio-pharmaceuticals, which appears to be capable of appropriate post-translational modifications of recombinant proteins. The mammary gland tissue specific vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals. In this study, to minimize physiological disturbance caused by constitutive over-expression of the exogenous gene, we constructed new retrovirus vector system designed for mammary gland-specific expression of the hEPO gene. Using piggyBac vector system, we designed to express hEPO gene under the control of mammary gland tissue specific and lactogenic hormonal inducible goat ${\beta}$-casein or mouse Whey Acidic Protein (mWAP) promoter. Inducible expression of the hEPO gene was confirmed using RT-PCR and ELISA in the mouse mammary gland cells treated with lactogenic hormone. We expect the vector system may optimize production efficiency of transgenic animal and reduce the risk of global expression of transgene.