• 한국연초학회지
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• 제20권1호
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• pp.50-56
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• 1998

The complementary DNA (cDNA) of potato virus Y- vein necrosis strain (PVY-VN) replicase gene (Nlb) was transformed into tobacco (Nicotiana tabacum cv. Burley 21) plants. Out of 25 putative transformants regenerated, 3 were resistant to PVY-VN, one highly resistant plant with no symptom until seed harvest time and the other two with mild chlorotic spot symptoms at late stages after infection. No symptom was observed in the highly resistant plant, while mild vein necrotic symptoms were developed on suckers of the moderately resistant plants after seed harvest time, In the first generation (T1) via self fertilization, resistance to susceptibility frequency in transgenic plants from the highly resistant transformant was about 3 : 1, while it was lowered much (about 1:2 and 1:19) in T1 of the moderately resistant transformants. In the second generation (T2) of the highly resistant plant, resistance frequencies were similar to T1, but resistance levels varied greatly and appeared to be decreased. Key words : potato virus Y, viral replicate gene, transgenic tobacco plants, resistance.

• Journal of Applied Biological Chemistry
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• 제49권3호
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• pp.75-81
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• 2006

A full-length cDNA encoding dihydroflavonol 4-reductase (st-dfr) of potato was isolated by rapid amplification of cDNA ends, and their expression was investigated from purple-fleshed potato (Solanum tuberosum L. cv. Jashim). The st-dfr exists as a member of a small gene family and its transcripts was abundant in the order of tuber flesh, stem, leaf, and root. The expressions of st-dfr gene were light inducible and cultivar dependant. Transgenic potato plants harboring antisense st-dfr (AS-DFR) sequences were analyzed. The accumulation of mRNA was nearly completely inhibited as a result of introducing an AS-DFR gene under the control of the 35S CaMV promoter into the red tuber skin Solanum tuberosum L. cv. Desiree. The anthocyanin content of the tuber peels of the transgenic lines was dramatically decreased by up to 70%. The possible production of flavonols in the peels of AS-DFR transgenic potatoes was discussed.

• The Plant Pathology Journal
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• 제23권4호
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• pp.300-307
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• 2007

Previously, a chloroplast-localized Cu,Zn superoxide dismutase (chCu,ZnSOD) was isolated from lily and the sense- and antisense- sequences of the lily chCu,ZnSOD were used to transform potato plants. Two selected lines, the sense- and anti-sense strand of transgenic plants, were further characterized for resistance to Erwinia carotovora, which is a severe pathogen affecting potato plants. Only the sense-strand transgenic potato, which contained less $O_2^{.-}$ and more $H_2O_2$ than wild-type and antisense-strand transgenic plants, showed increased resistance to E. carotovora. Additional studies using $O_2^{.-}$ or $H_2O_2$ scavengers in wild-type, sense-strand, and antisense-strand transgenic plants suggest that resistance to E. carotovora is induced by reduced $O_2^{.-}$ and is not influenced by $H_2O_2$. To the best of our knowledge, this report is the first study suggesting that resistance to E. carotovora is enhanced by reduced $O_2^{.-}$, and not by increased amounts of $H_2O_2$.

• 식물조직배양학회지
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• 제26권3호
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• pp.177-182
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• 1999

Chitinase 유전자가 삽입된 감자 Belchip 품종의 형질전환체와 대조구에 곰팡이 병원균을 접종하였다. 7개 계통의 형질전환식물체를 12cm정도 키운 후 병원균인 Phytophthora infestans의 zoospore를 접종하여 인공적으로 역병을 유발시켰다. 그 결과 발병율에 따라서 세 그룹으로 분리되었는데 대조구에 비하여 감염 정도가 심한 것 2개 계통. 비슷한 3개 계통, 발병 정도가 약한 2개 계통으로 구분되었다. 대조구에 비하여 저항성이 높았던 2개 계통과 발병이 심했던 1개 계통만을 대상으로 하여 2차실험을 실시한 결과, 1차실험과 비슷한 경향을 나타냈다. 포장에서 생육된 2개의 저항성 계통은 자연적으로 발생한 역병에 대해서도 역시 대조구에 비하여 역병저항성이 더 높았다.

• Plant Biotechnology Reports
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• 제2권1호
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• pp.13-20
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• 2008

Potato (Solanum tuberosum L.), one of the most important food crops, is susceptible to a number of devastating fungal pathogens in addition to bacterial and other pathogens. Producing disease-resistant cultivars has been an effective and useful strategy to combat the attack of pathogens. Potato was transformed with Agrobacterium tumefaciens strain EHA101 harboring chitinase, (ChiC) isolated from Streptomyces griseus strain HUT 6037 and bialaphos resistance (bar) genes in a binary plasmid vector, pEKH1. Polymerase chain reaction (PCR) analysis revealed that the ChiC and bar genes are integrated into the genome of transgenic plants. Different insertion sites of the transgenes (one to six sites for ChiC and three to seven for bar) were indicated by Southern blot analysis of genomic DNA from the transgenic plants. Expression of the ChiC gene at the messenger RNA (mRNA) level was confirmed by Northern blot analysis and that of the bar gene by herbicide resistance assay. The results obviously confirmed that the ChiC and bar genes are successfully integrated and expressed into the genome, resulting in the production of bialaphos-resistant transgenic plants. Disease-resistance assay of the in vitro and greenhouse-grown transgenic plants demonstrated enhanced resistance against the fungal pathogen Alternaria solani (causal agent of early blight).

• 식물병연구
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• 제9권1호
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• pp.1-9
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• 2003

The occurrence of potato(Sotanum tuberosum) viral diseases caused by Potato virus X(PVX), Potato virus Y (PVY), Potato leafroll virus(PLRV), Potato vims S(PVS), Potato virus M(PVM), Potato virus A(PVA), Potato virus T(PVT), Alfalfa mosic virus(AIMV), Tobacco mosic virus(TMV), Potato mop top virus(PMTV) Tobacco rattle virus(TRV) and Potato spindle tuber viroid(PSTVd), potato witches' broom phytoplasma, have been identified so far in Korea. Major viral diseases such as PVX, PVY and PLRV had been studied more deeply, however, the others are just identified and only partially characterized since the first study on the relation between PVX nucleic acid and virus protein by Kim in 1961. The most studies on potato viral diseases are mainly focused on the problems of seed potato production. The National Alpine Agricultural Experiment Station(NAAES), since it began its activities in 1961, has given special attention to this problem by doing studies to identify, characterize and control potato virus diseases. This effort resulted in the development of new potato virus detection methods as a basis for elaborating new method of control, such as the production of seed potato free of virus and the selection of new virus-resistant transgenic potatoes. The further studies of potato viral diseases required would be fallowings: the continuous monitoring for the occurrence of identified or not identified potato viruses in Korea, the isolation of resistant viral genes, the development of control method for the non-persistently transmitted viruses like PVY, special vectors such as nematode and fungus transmitted viruses, TRV and PMTV and the development of control methods against potato viral diseases by viral cross protection, therapy, transgenic plant, and the use of the agents or molecules, such as virus inhibitors and antiviral proteins, etc., blocking viral replication.

• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.1027-1031
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• 2010

To examine the possibility of horizontal gene transfer between transgenic potatoes and microorganisms in potato fields, the gene flow from transgenic potatoes containing the nucleoside diphosphate kinase 2 (NDPK2) gene to microorganisms in soils was investigated. The soil samples collected from the potato fields from March to October 2007 were examined by PCR, Southern hybridization, and AFLP fingerprinting. The NDPK2 gene from soil genomic DNAs was not detected by both PCR and Southern hybridization, indicating that gene transfer did not occur in the potato fields. In addition, no discrepancy was found in pathogenicity and noticeable changes for the appearance of variants of Phytophthora infestans in each generation when serial inoculations and the analysis of genomic DNAs by AFLP were conducted. Thus, these data suggest that transgenic potatoes do not give significant impacts on the communities of soil microorganisms and the emergence of variants, although continued research efforts may be necessary to make a decisive conclusion.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.223-230
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• 2016

아스코르빈산(ascorbic acid, AsA)는 강력한 항산화 물질 및 환원제로서 식물에서 산화형 AsA인 dehydroascorbate(DHA)를 활성형인 환원형 AsA로 변환시키는 효소인 dehydroascorbate reductase (DHAR)에 의해 생성된다. 선행연구의 결과로서, 본 연구팀은 참깨의 모상근에서 분리한 DHAR 유전자를 이용하여 항시 발현하는 CaMV 35S 프로모터와 괴경 특이적으로 발현하는 patatin 프로모터의 조절하에 과발현 시킨 형질전환 감자를 개발하였다. 형질전환 감자 식물체들은 비형질전환체 보다 증가된 DHAR 활성과 AsA 함량을 보인 바 있다. 본 연구에서는 단백질체 분석을 통해서 형질전환 감자 식물체에서 DHAR 과발현에 의해 조절되는 단백질들을 조사하였다. 단백질체 분석의 결과로서, 형질전환 감자에서 도입된 DHAR 단백질과 다양한 항산화 관련 단백질들이 식물 생장시기 동안 증가 하였다. 본 연구의 결과로서, 도입된 유전자인 DHAR이 항산화 단백질들의 발현 증가를 통해 형질전환 식물체의 스트레스 내성 기작을 향상 시킬 수 있을 것으로 생각된다.

• Journal of Plant Biotechnology
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• 제45권4호
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• pp.364-368
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• 2018

R2R3 유형의 단백질인 IbMYB1 전사인자는 고구마의 뿌리에서 안토시아닌 생합성 과정을 조절하는 중요한 단백질이다. 선행연구에서 IbMYB1 유전자의 발현 증가를 통한 안토시아닌의 합성 증가가 담배, 애기장대 및 고구마의 저장뿌리에서 증명된 바 있다. 본 연구에서는 괴경(저장줄기) 특이적 PATATIN 프로모터와 산화스트레스 유도성 SWPA2 프로모터의 조절하에서 안토시아닌을 고함유하는 IbMYB1 유전자 과발현 형질전환 감자를 개발하여 그 특성을 분석하였다. PAT-IbMYB1 형질전환 식물체들은 대조구 식물체 및 SWPA2-IbMYB1 식물체 보다 높은 안토시아닌 함량을 괴경에서 나타내었다. 본 연구의 결과로서, IbMYB1의 과발현은 형질전환 기술을 이용한 특정 조직 특이적 안토시아닌 생산에 매우 좋은 개발 기술이 될 것으로 생각되는 바이다.

• Journal of Plant Biotechnology
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• 제29권4호
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• pp.229-233
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• 2002

식물의 항산화력을 증진하여 식물병원균 저항성 작물을 개발하고자, 철분 결합 단백질인 대두의 ferritin 유전자를 CaMV 35S와 hsr203J promoter에 연결하여 감자에 형질전환하였다. PCR및 Northern분석에 의한 형질전환 감자에 ferritin 유전자가 존재하는 것과 이들 유전자 식물체내에서 발현되는 것을 확인하였다. ferritin 유전자를 담배 유래 병원균 특이 발현promoter인 hsr203J와 연결하여 획득된 형질전환 감자 식물체는 감자역병균 접종 후 24시간대에서 전사체 발현량이 가장 많았으며 그 후 줄어드는 경향을 나타내었다. 유전자 도입이 확인된 형질전환체 감자괴경의 철분 함량은 CaMV 35S와 ferritin 유전자 도입 형질전환체가 2.5배, hsr203J promoter와 ferritin 유전자 도입 형질전환체가 1.5배 각각 증가하는 것으로 나타냈다. 또한 이들 형질전환체는 감자 무름병균에 대한 저항성 증진효과를 관찰할 수 있었다.