• Journal of Embryo Transfer
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• v.16 no.3
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• pp.223-231
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• 2001

The success of nuclear transplantation with mammalian oocytes depends critically on the potential of oocytes activation, which mainly caused to prevent the re-accumulation of maturation promoting factor (MPF). This study was conducted to compare the effect of combined treatment of lonomycin with a Hl-histone kinase inhibitor (dimethylaminopurine, DMAP) or cdc2 kinase inhibitor (sodium pyrophosphate, SPP) on activation of bovine oocytes. In vitro matured bovine oocytes with the first polar body (PB) and dense cytoplasm were assigned to 3 experimental groups. For activation treatment, oocytcs were exposed to 5 $\mu$M lonomycin for 5 min (Group 1), and followed by 1.9 mM dimethylaminopurine (DMAP) for 3 h (Group 2) or followed by 2 mM sodium pyrophosphate (SPP) for 3 h (Group 3). The activation effects in the three treatments and the control group (untreated) were judged by the extrusion of the second PB and formation of a pronucleus (PN). Differences among groups were analysed using one-way ANOVA after arc-sine transformation of proportional data. All three treatments led to high activation rates (90% to 95%), with significant difference from the control. However, the extrusion of the second PB and the rate of PN formation differed remarkably among treatments. In Group I and 3, about 95% of the oocytes had extruded the second polar body, but one PN had formed in a higher proportion of oocytes in Group 3 than in Group 1 (90% vs. 5%). In experiment 2, the rates of cleavage and development into blastocysts in Group 1 were significantly lower than those of Group 2 and 3 (8.7% and 0% vs. 50.5% and 11.6%, and 44.6% and 7.2%, respectively, P<0.05). In experiment 3, ~80% of parthenotes in Group 1 were developed with haploid chromosomal sets. However, when ionomycin was followed immediately by DMAP (Group 2). only 20% of parthenotes were haploid. In Group 3, combined treatment with ionomycin and SPP, the appearance of abnormal chromosomal tracts was significantly (P〈0.05) reduced and the proportion of haploid parthenotes was increased to 85% (17/20) than in Group 2. These results demonstrate that SPP acted as a cdc2 kinase inhibitor and formed the haploidy in oocyte activation. Thus, the present study suggests that cdc2 kinase inhibitor, such as sodium pyrophosphate, may have an effective role in oocyte activation for the production of cloned embryos/animals by nuclear transplantation.

• Journal of Popular Narrative
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• v.25 no.4
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• pp.223-261
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• 2019

This article is a reexamination of the feminist criminal narrative I wish for what is forbidden by Yang Gui-ja in the context of the rise of the women's movement and consumer culture of the middle class in Gangnam in the 1980s and 1990s. At this time, the explosive media culture served to strengthen the ideology that placed the middle-class family at the center as well as the consumption culture. The combination of consumer media culture, women's movement and democratization created a soft and domestic male image while visualizing the material foundation of the middle class in the 1990s of South Korea. In this novel, the domestic male image transforms the feminist criminal narrative into the narrative of the femme fatale attacking the stability and dignity of the middle class family, and at the moment of the transformation, the feminist woman Kang Min-ju is killed by a lower class man who has admired and loved her. This novel is not only current but also signifying as a text that overlaps sociocultural reproduction and feminist issues of the middle class based on Gangnam in the 1990s. This is because it shows the sociocultural context of femicide, such as serial murder of targeting women, as a core code of criminal narrative to be held in Korea since the late 1990s.

• Journal of the Korean Society for information Management
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• v.40 no.2
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• pp.211-241
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• 2023

With rapidly changing technological implementation of operating systems of businesses, the Ministry of foreign affairs (MOFA) of the Republic of Korea (ROK) has been undergoing digital transformation to its overall operations with the intent to innovate information and knowledge management (IKM) strategies since the mid-2000s. However, assessment as to the effectiveness of implemented IKM has been inadequately analyzed. This study aims to assess the concepts and limitations of the MOFA's current IKM strategies and the methods it employs to deliver its IKM framework, in light of strengthening the organizational ambidexterity and absorptive capacity, and also fostering organizational innovation through a qualitative study that involves interviews and analysis of reports from MOFA. The MOFA's IKM possesses dynamic capabilities to adapt to changing digital technologies. However, the institution's IKM is constrained by limitations associated with the utilization of the IKM system such as a structure that handles confidential documents and a lack of a collaborative system for IKM, and external limitations such as changes in the domestic political situation governing MOFA's priorities and the hierarchy of government organizations. Consequently, developing the organizational ambidexterity and absorptive capacity was not possible. To develop an IKM framework for organizational innovation, the MOFA must devise a way to minimize the impact of external changes by overcoming internal limitations. To that end, a detailed study on the development of a practically usable IKM system should include establishing a dialogue between job groups and enhancing employee competency in preparation for a changing environment.

• Science of Emotion and Sensibility
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• v.26 no.1
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• pp.65-78
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• 2023

Aircraft are representative of human-machine systems. There is a delay between the human operation and the completion of the machine operation such as when the machine starts to operate and when the force is transmitted to the machine and completed. Time perception is an important component of timing tasks and is known to be affected by the anxiety associated with high arousal. This research verified the impact of weather, flight, and time conditions on the anxiety and time perception of in-service pilots in a virtual reality area. Weather conditions were divided into visual flight weather conditions and very low visibility conditions. Experiments 1 and 2 were performed with different flight and time conditions. In Experiment 1, time perception was measured by employing a button added to the control rod in the scenario of hovering and level flight with relatively little transformed in momentum and little delay. In Experiment 2, time perception was measured in the procedure of naturally taking off the helicopter by employing only the control stick in a takeoff scenario where there was a lot of transformation in momentum and a lot of delays. As a result of the experiment, it was reported that anxiety and heart rate increased in very low visibility conditions In particular, among all flight conditions in Experiments 1 and 2, it was reported that time was overestimated in the scenario of increased anxiety. This outcome can lead to overestimation of time under the impact of anxiety and failure of the timing task, which may lead to challenges in maneuvering and possibly lead to accidents.

• Journal of Integrative Natural Science
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• v.16 no.1
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• pp.13-31
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• 2023

The sesarmid crab Muradium tetragonum, inhabiting the mangrove, are considered as a key consumer of litter and thereby play an important role in the detritus food chain and energy flow in the mangrove ecosystem. The present investigation was carried out with objectives to enlighten the reproductive system of Muradium tetragonum through histological and histochemical studies. Histological organization of the testis of M. tetragonum revealed that each testis has a lobular structure consisting of several testicular lobules arranged around the collecting duct. Histology of the deferens of M. tetragonum revealed it to be composed of three-layer of tissues along the entire length:the outer connective tissue, the middle muscular and the inner epithelial layer. Based on the histological architecture these three regions are recognized as proximal vas deferens (PVD), middle vas deferens (MVD) and distal vas deferens (DVD). Histological characteristics of the ovary of M. tetragonum during different phases of ovarian development were studied. Based on the colour changes of the ovary and diameter of the oocytes five stages of ovarian development can be pronounced. Histochemical analysis of the male reproductive tissues of M. tetragonum signifies the secretion of a different biomolecule by specifying their origin in the reproductive tissue and their possible transformation into spermatophores. In the female reproductive tissues, histochemical evaluation envisaged the secretory products during different stages of ovarian development The secretory substances of the spermatheca expound on the significance of its secretion in dehiscing the spermatophore wall and in nourishing as well as protecting the spermatozoa.

• Environmental and Resource Economics Review
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• v.32 no.2
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• pp.127-147
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• 2023

This study is trying to analyze the economic effect of replacing thermal power generation, one of the government's carbon-neutral policies, with new and renewable energy. For this analysis, scenario A is set to replace 100% of thermal power generation with new and renewable energy, and scenario B is set to replace 60% of thermal power generation with new and renewable energy. In addition, costs are incurred when replacing thermal power generation with new and renewable energy, and scenario 1 is the same cost as the current cost, and scenario 2 is120% higher than the current cost. Therefore, when converting thermal power generation to new and renewable energy, the scenarios are largely organized into four cases. In the case of replacing thermal power generation with new and renewable energy, the production inducement coefficient of thermal power generation decreased from the current level regardless of the scenario. However, the value-added inducement coefficient and the greenhouse gas emission inducement coefficient are lower than the current level when thermal power is converted to renewable energy by 100%, while the value-added inducement coefficient and greenhouse gas emission inducement coefficient are higher than the current level. In addition, the greenhouse gas emission induction coefficient of most industries was found to decrease, while the production induction coefficient and the value-added induction coefficient increased. Scenario A seems appropriate because the purpose of the government's policy is to reduce greenhouse gas emissions by converting thermal power into new and renewable energy. However, as a result of this, the production inducement coefficient and value-added inducement coefficient of some industries decrease, so the government's support policy is needed to solve this problem

• Journal of the Korean Society of Food Culture
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• v.24 no.1
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• pp.84-89
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• 2009

This paper was conducted to evaluate aglycones and carbohydrates produced by acid hydrolysis of three potato glycoalkaloids [(PGA); ${\alpha}$-chaconine, ${\alpha}$-solanine, and demissine] in potatoes. Standard solanidine and demissidine were dissolved in 1N HCl and then heated at $100^{\circ}C$ for 10-120 min. Solanidine was rapidly decomposed during acid hydrolysis and one peak that was identified as solantherene ($M^+$=379) by GC-MS was detected. The transformation solanidine to solanthrene was approximately 50% complete after 10 min, approximately 90% complete after 60 min and 100% complete after 120 min. Demissidine was hydrolyzed using the same method that was used to hydrolyze the solanidine. However, demissidine produced only one peak upon GC-MS ($M^+$=399) analysis and was found to be very stable at increased temperatures. Acidy hydrolysis of ${\alpha}$-chaconine, ${\alpha}$-solanine and demissine resulted in the decomposition of ${\alpha}$-chaconine and ${\alpha}$-solanine to solanidine and solanthrene, respectively. Therefore, this hydrolysis method should not be utilized to produce PGA combining with solanidine as aglycone. The individual carbohydrates produced by the two PGAs by hydrolysis were very stable at increased temperatures; therefore, it was possible to quantify these PGAs based on calculation of the individual carbohydrate content. Conversely, because demissidine produced by the hydrolysis of demissine was extremely stable at increased temperatures, it was possible to quantify the PGA based on the aglycone produced by hydrolysis.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.638-643
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• 2004

소형화된 기계가공시스템은 사용재료의 다양화와 에너지 및 공간의 감소와 같은 장점을 가지고 작고 정밀한 부품을 가공할 수 있는 시스템으로 주목받고 있다. 이러한 시스템이 비록 그 크기가 일반적인 가공시스템에 비해 작지만 정렬 및 조립공정, 기계요소의 불완정성에 의한 기하학적 오차는 여전히 존재한다. 기하학적 오차 평가는 기계시스템의 정밀도를 효과적으로 적은 비용으로 향상시킬 수 있는 오차보정기술을 적용할 수 있는 토대가 된다. 일반적으로, 3 축의 직선축으로 이루어진 공작기계는 21 개의 오차요소를 가진다. 레이져간섭계는 이러한 오차요소를 평가하는데 널리 사용되고 있지만 광학계를 정렬하고 설치하는 데 많은 어려움이 있으며 한번의 설치로 한 개의 오차요소만이 측정 가능하다. 또한, 소형공작기계의 경우, 그 크기로 인해 기존의 레이져 간섭계를 직접적으로 적용할 수 없다. 따라서, 본 연구에서는 소형공작기계를 포함한 소형가공시스템의 기하학적 오차 평가를 위한 새로운 다자유도 측정시스템을 제안하였다. 5 개의 정전용량변위센서를 사용하는 이 시스템을 통해 한 축의 움직임에 따른 5 개의 오차요소를 동시에 측정 가능하다. 균질 변환행렬을 이용한 측정알고리듬을 구성하고 이를 모의시험을 통해 평가하였다. 수학적 모델링을 통해 각 센서의 출력값을 유도하고 이를 이용하여 각 오차요소를 계산하기 위한 식을 유도하였다. 여기서, 단순화된 식을 적용한 경우, 임의의 오차에 대한 측정 알고리듬의 정확도를 평가하였다. 또한, 측정 시스템의 설치시 발생하는 셋업오차에 대한 측정 알고리듬의 민감도 분석을 행하였다. 제안하는 측정 시스템은 구조가 간단하고 고가의 부가장비가 필요치 않다. 또한, 적은 비용으로 구성할 수 있으며 높은 측정 정밀도를 가지고 소형가공시스템에 필요한 오차 평가를 행할 수 있다.가 함유된 계란을 생산하고 섭취하였을 때 특정항체들의 결합을 통해 병원성 미생물의 성장이나 군체를 형성하는 것을 무력화시켜 결과적으로 병원균을 감소시키거나 억제시킨다는 점이다. 오늘날 약물에 내성을 지닌 박테리아의 출현으로 질병감염을 막는데 항생제의 사용효과가 점차 감소하고 있기 때문에 이러한 항생제를 대체할 수 있는 방안으로 계란항체를 이용할 수 있다.한 중공 플랜지 형상의 단조 방법 중 보다 적절한 단조방법인 압조 단조에 있어서 일반적으로 사용되고 있는 SM10C에 대한 유한요소 해석을 수행하였으며, 제품의 형상비에 따라 폴딩 결함의 발생 유무를 검토하고, 폴딩 결함 없이 단조하기 위한 중공 플랜지의 형상한계 비를 제시하였다.도 경미하게 나타났으나, 경련이 나타난 쥐에서는 KA만을 투여한 흰쥐와 구별되지 않았다. 이상의 APT의 항산화 효과는 KA로 인한 뇌세포 변성 개선에 중요한 인자로 작용할 것으로 사료되나, 보다 명확한 APT의 기전을 검색하고 직접 임상에 응응하기 위하여는 보다 다양한 실험 조건이 보완되어야 찰 것으로 생각된다. 항우울약들의 항혈소판작용은 PKC-기질인 41-43 kD와 20 kD의 인산화를 억제함에 기인되는 것으로 사료된다.다. 것으로 사료된다.다.바와 같이 MCl에서 작은 Dv 값을 갖는데, 이것은 CdCl$_{4}$$^{2-}$ 착이온을 형성하거나 ZnCl$_{4}$$^{2-}$ , ZnCl$_{3}$$^{-}$같은 이온과 MgCl$^{+}$, MgCl$_{2}$같은 이온종을 형성하기 때문인것 같다. 한편 어떠한 용리액에서던지 NH$_{4}$$^{+}$의 경

• Journal of Service Research and Studies
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• v.11 no.2
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• pp.67-77
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• 2021

This study is a study on the methodology of establishing an innovation strategy centering on the customer experience, which is essential in order to transform the existing collection and preservation-centered mutual aid company service into a visitor-centered service. To this end, we conducted literature research on environmental changes in the funeral industry from the perspective of service science and the significance and value of customer experiences within them, good customer experiences and bad customer experiences from the perspective of customer experience management. A study was conducted to present and prove a specific model. The customer experience-oriented innovation strategy of the funeral industry means to search for various alternatives that can reach the target state from the present state, focusing on the customer, and select the most appropriate transformation plan among them. As an effect of application, it was found that it is a source of differentiation by generating positive emotions to customers, and that customer experience data is highly helpful in making important decisions for the actual resource input of the parent company. This innovation model was presented, and its value was firstly proved by analyzing the difference from the existing evaluation method. Finally, as a result of analyzing the causal relationship through regression analysis using the customer experience measurement procedure, customer experience diagnosis/evaluation, customer experience innovation strategy, and cooperative company's performance as variables, the relationship proved to be significant.

• Journal of Service Research and Studies
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• v.11 no.2
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• pp.21-36
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• 2021

This study investigated the means-end chain theory more concretely through the APT hard laddering technique. This is carrying out a questionnaire survey targeting users by hair salon type, and the items drawn from the qualitative laddering technique are applied. The technique is a comparative analysis of each attribute, consequences, and value item by analyzing each step's questions. The results are as follows. First, hairdresser's ability, acceptance of individual-customized opinions, and cheap price were the most mentioned items in the selection attributes. As for the consequences items, image transformation, neatness, novelty, and psychological stability were drawn in order. The items indicated as important among the value items were satisfaction, followed by happiness, confidence, beauty, and bond. Second, the remarkable selection attributes, irrelevant of hair salon type, was revealed as hairdresser's ability and the key values pursued when using a hair salon were drawn as satisfaction, confidence, and beauty. From this result, it was found that meeting the desire of consumers using hair salons can be linked with ultimately pursued values. It was also verified that partial differences were shown by hair salon type and this meant that consumers' desire and expected benefits were different by hair salon type. Although this study drew value perception through comparison with hair salon types based on the means-end chain theory, it was confirmed that the most important selection attribute was hairdresser's ability and they select and use hair salons to gain satisfaction and confidence.