• Title/Summary/Keyword: transformation region

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Transformation of Rabbit Proximal Tubule Cells by Strontium Phosphate Transfection with a Plasmid Containing SV4O Early Region Genes

  • Han, Ho-Jae;Taub, Mary L.
    • The Korean Journal of Physiology
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    • v.28 no.2
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    • pp.233-240
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    • 1994
  • In this study, it was investigated whether immortalized proximal tubule cells transformed with pRSVT could survive through the numerous passages. Results were as follows: 1. The cells transfected with pRSVT formed rapidly growing, multilayered colonies within 2 weeks in a hormone defined medium. Domes were also observed in some of the cultures. 2. r-glutamyl transpeptidase activity was equivalent to that observed in primary renal proximal tubule cell cultures. 3. Transformed cells with pRSVT form tubules in matrigel following 20 passages. 4. Genomic DNA of transformants was digested with either the restriction enzyme Xba or BamH1. A band of approximately 7.5kb was detected with Xba. Three BamH1 bands were detected at approximately 15 kb, 6.5 kb, and 3 kb.

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Evaluation of Analytical Vibration Characteristics for Triple Cylindrical Shells Filled with Fluid (유체로 채워진 삼중 원통셸의 해석적 진동 특성 평가)

  • 지용관;이영신
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.12 no.2
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    • pp.150-160
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    • 2002
  • The free vibration characteristics of the triple cylindrical shells filled with fluid are investigated. The triple cylindrical shells are filled with compressible fluid. The boundary condition is clamped at both ends. Analytical method is developed to evaluate natural frequencies of triple cylindrical shells using Sanders' shell theory and courier series expansion by Stokes' transformation. Their results are compared with those of finite element method to verify the validation of the method developed. The modal characteristics of shells filled with fluid at region 1, 2 and 3 are evaluated.

Effect of the Heat Treatment Parameters on the Phase Transformation and Corrosion Resistance of Fe-14Cr-3Mo Martensitic Stainless Steel

  • Park, Jee Yong;Park, Yong Soo
    • Corrosion Science and Technology
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    • v.6 no.2
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    • pp.56-61
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    • 2007
  • Carbide dissolution during heating processes can change chemical composition of martensitic stainless steel in its austenitic phase. Although the austenitizing treatments were carried out at a homogeneous austenite region, the amount of carbon atom in the matrix differs. Increase in the amount of carbon contents in the matrix resulted in decreasing MS temperature, which consequently causes the volume fraction of the retained austenite to increase. This study reveals the effects of the austenitizing treatment on the properties of Fe - 0.3C - 14Cr - 3Mo martensitic stainless steel change with different austenitizing temperatures.

Elliptic Numerical Wave Model Solving Modified Mild Slope Equation (수정완경사방정식의 타원형 수치모형)

  • YOON JONG-TAE
    • Journal of Ocean Engineering and Technology
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    • v.18 no.4 s.59
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    • pp.40-45
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    • 2004
  • An efficient numerical model of the modified mild slope equation, based on the robust iterative method is presented. The model developed is verified against other numerical experimental results, related to wave reflection from an arc-shaped bar and wave transformation over a circular shoal. The results show that the modified mild slope equation model is capable of producing accurate results for wave propagation in a region where water depth varies substantially, while the conventional mild slope equation model yeilds large errors, as the mild slope assumption is violated.

Arrival at the Caspian Coast: Migration, Informality and Urban Transformation in Sumqayit, Azerbaijan

  • JAGER, PHILIPP FRANK
    • Acta Via Serica
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    • v.5 no.2
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    • pp.51-90
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    • 2020
  • The city of Sumqayit in Azerbaijan was famous in the USSR for its chemical plants, which supplied the whole country with plastics, detergents, and fertilizers. While production increased in the post-WWII period, young people from remote Caucasian villages were attracted as workers to the industrial settlement on the shore of the Caspian Sea and worked together with specialists from all over the USSR. Migration did not stop when the USSR collapsed. To the contrary, mobility increased as Azerbaijani refugees from Armenia and IDPs from Karabakh fled to Sumqayit, which grew to become the second-largest city of Azerbaijan. Although a generation has passed since the ceasefire, IDPs still are separately administered. In the last 20 years, more and more internal migrants have chosen the Greater Baku Region as their destination, mostly finding jobs in the informal labor market. In the post-independence transformative period, informal housing has offered migrants a place to stay in the city. Sumqayit can be regarded as an arrival city, an established urban platform for migrants who prefer internal over transnational migration.

Pseudo-Distance Map Based Watersheds for Robust Region Segmentation

  • Jeon, Byoung-Ki;Jang, Jeong-Hun;Hong, Ki-Sang
    • Proceedings of the IEEK Conference
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    • 2001.09a
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    • pp.283-286
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    • 2001
  • In this paper, we present a robust region segmentation method based on the watershed transformation of a pseudo-distance map (PDM). A usual approach for the segmentation of a gray-scale image with the watershed algorithm is to apply it to a gradient magnitude image or the Euclidean distance map (EDM) of an edge image. However, it is well known that this approach suffers from the oversegmentation of the given image due to noisy gradients or spurious edges caused by a thresholding operation. In this paper we show thor applying the watershed algorithm to the EDM, which is a regularized version of the EDM and is directly computed form the edgestrength function (ESF) of the input image, significantly reduces the oversegmentation, and the final segmentation results obtained by a simple region-merging process are more reliable and less noisy than those of the gradient-or EDM-based methods. We also propose a simple and efficient region-merging criterion considering both boundary strengths and inner intensities of regions to be merged. The robustness of our method is proven by testing it with a variety of synthetic and real images.

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Effect of Composition in Cu-Al-Mn Shape Memory Alloys on the Shape Memory Properties and Cold Workability (Cu-Al-Mn계 형상기억합금에서 조성이 형상기억특성 및 냉간가공성에 미치는 영향)

  • Park, Jong Bae;Park, Hyun Gyoon
    • Journal of the Korean Society for Heat Treatment
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    • v.27 no.2
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    • pp.59-64
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    • 2014
  • Cu-Al-Mn shape memory alloys of a variety of composition were characterized in terms of shape memory properties and cold workability. Cold workability tested by cold rolling indicated that the alloys solution treated in the ${\alpha}+{\beta}$ region have a higher ductility than those solution treated in the ${\beta}$ region. Also it is known that cold workability increased with the decrease in Al content in the ${\beta}$ region. This seems to be resulted from the fact that Mn addition causes to expand ${\beta}$ region toward lower Al content and lower order-disorder transition temperature, consequently, ${\beta}$ of excellent workability being frozen even at room temperature. Experimental results regarding shape memory showed that the properties were better with a higher Al contents at a given Mn content, which is closely related with martensitic transformation. It is also shown that super elasticity limit was enhanced with decrease in the yield strength of alloys because a lower yield strength seems to initiates slip at the lower applied stress.

Molecular Control of Gene Co-suppression in Transgenic Soybean via Particle Bombardment

  • El-Shemy, Hany A.;Khalafalla, Mutasim M.;Fujita, Kounosuke;Ishimoto, Masao
    • BMB Reports
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    • v.39 no.1
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    • pp.61-67
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    • 2006
  • Molecular co-suppression phenomena are important to consider in transgene experiments. Embryogenic cells were obtained from immature cotyledons and engineered with two different gene constructs (pHV and pHVS) through particle bombardment. Both constructs contain a gene conferring resistance to hygromycin (hpt) as a selective marker and a modified glycinin (11S globulin) gene (V3-1) as a target. sGFP(S65T) as a reporter gene was, however, inserted into the flanking region of the V3-1 gene (pHVS). Fluorescence microscopic screening after the selection of hygromycin, identified clearly the expression of sGFP(S65T) in the transformed soybean embryos bombarded with the pHVS construct. Stable integration of the transgenes was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Seeds of transgenic plants obtained from the pHV construct frequently lacked an accumulation of endogenous glycinin, which is encoded by homologous genes to the target gene V3-1. Most of the transgenic plants expressing sGFP(S65T) showed highly accumulation of glycinin. The expression of sGFP(S65T) and V3-1 inherits into the next generations. sGFP(S65T) as a reporter gene may be useful to increase the transformation efficiency of transgenic soybean with avoiding gene co-suppression.

The impact analysis of interface crack in dissimilar materials using the 2-D laplace transformed BEM (2차원 Laplace 변환 경계요소법에 의한 이종재료 접합면 균열의 충격해석)

  • 김태규;조상봉;최선호
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.18 no.5
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    • pp.1158-1168
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    • 1994
  • For BEM analyses of the impact problems of dissimilar materials, the connected multi-region method using perfect bonded conditions on the interface boundaries was added to two-dimensional Laplace transformed-domain BEM program for a single region analysis. It was confirmed that the BEM results of impact problems of a single-region and multi-regions for a homogeneous isotropic material are agreed well. The two-dimensional Laplace transformed-domain BEM program combined with connected multi-region method was applied to analyse several impact problems of dissimilar materials. Also the feasibility of BEM impact analyses was investigated for dissimilar materials by the analysis of the BEM results for impact problems of dissimilar materials in terms of physical aspects. As for an application, the two-dimensional Laplace transformed BEM concerning impact problems of cracks at the interface of dissimilar materials and the determinating process of the dynamic stress intensity factors by extrapolation method are presented in this paper.

Comparative Review of the Groves for making enclosed Village and Village Groves for making Rest Place in Rural Human Settlement Region -Centered on Chinan-Kun Region- (농촌정주생활권내의 마을비보숲과 마을쉼터숲의 비교고찰: 진안군 지역을 사례로)

    • Journal of the Korean Institute of Landscape Architecture
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    • v.27 no.3
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    • pp.32-38
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    • 1999
  • This paper search the difference between the village groves for enclosing and the village groves for rest in Chinan-Kun region. Both charged 72.5%(37 groves) of all(51 groves). The form of village groves for rest was various, but that of those for enclosing was linear. The average area of village groves for enclosing was 2,755.4$m^2$ and that of those for rest was 661.2$m^2$. So village groves for enclosing was 4.2 times larger than that of those for rest. In function of village groves, it was identified that function of complementing vacant part was more important than of rest in human settlement region. In the transformation of village groves, the village groves for enclosing were transformed into other productive use than those for rest. Although the worship service were performed annually in the past, that were almost disappeared in present. The forest state of village groves were almost deciduous ones. In vegetation structure, the number of upper trees of village groves for enclosing was 5 times more than that of those for rest. In average appearing rate of upper trees, the village groves for enclosing was 2 times more than that of those for rest. The most principal dominent species of both in upper trec was Zelkova serrata. The species showing highly appearing rate of upper trees were Zelkova serrata, Carpinus tschonoskii, Celtis sinensis, Qercus acutissima, Pinus densiflora, Castanea crenata, Salix glandulosa. etc.

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