• 대한영양사협회학술지
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• 제26권4호
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• pp.243-253
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• 2020

The purpose of this study was to set new guidelines for adenosine triphosphate (ATP) bioluminescence hygiene monitoring of distribution trays at children's foodservices. Five dietitians visited 223 foodservices (95 institutional, 128 small) to examine whether they adhered to the norms of 'Keeping distribution tray sanitary by washing/sanitizing' and 'Performing food distribution in a clean and appropriate way'. In this visit, dietitians swabbed 100 ㎠ area of the distribution trays twice, once for obtaining ATP measurements and the second time for Aerobic Plate Counts (APC) using 3M Petrifilm Plates. Chi-square test and ANOVA were applied using SPSS 23.0 software. SPSS 23.0 was used to conduct graphical and statistical analysis of the raw data of ATP measurements, which were further transformed by a Box-Cox transformation. The mean of APC from all the subjects inspected was 3.8×10²±2,102.0 CFU/100 ㎠. A total of 208 (93.3%) trays were observed within the acceptable limits of APC (Pass<5.0×10² CFU/100 ㎠). APCs taken at institutional foodservices showed significantly lower levels (1.4×10²±600.0 CFU/100 ㎠, P<0.01) compared to the small foodservices (5.5×10²±2,718.7 CFU/100 ㎠). No significant differences were observed between the two groups in ATP measurements and in the performance rate of 2 checklist items. As against the 93.3% APC adequacy from the total subjected inspection, total ATP adequacy (Pass≤300 RLU/100 ㎠) was only 71.7%. Therefore, more practical guidelines should be prepared for the assessment of the hygiene of distribution trays. In the graphical and statistical analysis, levels below 250 RLU/100 ㎠ was considered 'Pass', while equal to or greater than 350 RLU/100 ㎠ was considered 'Fail' for distribution trays.

• 감성과학
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• 제5권1호
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• pp.25-32
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• 2002

본 연구에서는 7가지 실크직물의 스치는 소리를 녹음한 후, 색채로 변환시켜 변환색채에 대한 감각과 감성을 평가하고, 2차원 모형을 제시하여 감각/감성과 직물의 관계를 시각화하였다. 피험자 30명을 대상으로 변환색채에 대한 감각과 감성을 평가하도록 하였으며 색채의 물리량은 홍색비율(RP), 녹색비율(GP), 청색비율(BP)과 색채 빈도수(CC)를 계산하였다. 감성차원에 대하여 활동감, 우아함, 터프함의 세 요인이 도출되었다. 활동감 차원은 GP, CC와는 정적인 상관을 보였으며 능직물과 견방사의 교직물을 가장 활동적이라고 평가하였다 우아함 차원은 RP와는 정적상관을, CC와는 부적상관을 보였으며 수자직물과 평직물이 가장 우아하다고 평가되었다. 터프함 차원은 RP와 높은 정적상관을 가졌다. 능직물과 같이 색채 빈도수가 많고 홍색비율과 녹색비율이 많은 변환색채를 선호하였다.

• 복식
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• 제53권8호
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• pp.65-80
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• 2003

The purpose of this study was to examine and fully understand the transformation of female's ideal beauty through time based on word analysis. Word selection was made from the very exemplary Women's magazines : Yuhwon, Jubu-Saengwhal, and Yuhsung-Dongah. Further, in order to research the understanding of female's ideal beauty, a random research was done on 445 women, ranging from the ages 20's through 60's. From the result, a frequency analysis was performed to further analyze the meaning of ideal beauty to each sector of the age groups for different time periods : the 1980's and the 1990's. To the 20's, beauty awareness was defined by the word ‘intelligence’, however, the 1980's endorsed confidence with composure and calmness where as the 1990's preferred beauty to be defined by ‘refinement and goal oriented’. The 30's selected ‘individuality’ marked with confidence in character, but there was a shift from ‘mature intelligence’ in the 1980's to ‘professionalism’ in the 1990's. The 40'E chose mediocrity and commonness, however, likewise, a sense of ‘earnestness’ for life and family was looked upon as beautiful. For the 50's, comfort with a reserved tenure was frequently noted and for the 60's, women chose ‘health and independence’ as describing ideal beauty. The results from the research are justifiable because the articles are in a neutral perspective and are from the representative women's magazines. This research proved that the younger the age group, the more exterior beauty was considered, as opposed to the older generation who found inner beauty to be of most importance.

• BMB Reports
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• 제44권12호
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• pp.793-798
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• 2011

Recently, pluripotency induction or cellular reprogramming by introducing critical transcription factors has been extensively studied, but has been demonstrated only in vitro. Based on reports that Oct4 is critically involved in transforming neural stem cells into pluripotent cells, we used the lentiviral vector to introduce the Oct4 gene into the hippocampal dentate gyrus (DG) of adult mice. We examined whether this manipulation led to cellular or behavioral changes, possibly through processes involving the transformation of NS cells into pluripotent cells. The Oct4 lentivirus-infused group and the green fluorescent protein lentivirus-infused group showed a similar thickness of the DG and a comparable level of synaptophysin expression in the DG. Furthermore, our behavioral analyses did not show any differences between the groups concerning exploratory activity, anxiety, or memory abilities. This first trial for pluripotency induction in vivo, despite negative results, provides implications and information for future studies on in vivo cellular reprogramming.

• 한국의류학회지
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• 제37권3호
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• pp.270-279
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• 2013

This study presents a T-shirt design process that utilizes modernized traditional patterns; in addition, it also includes various modernization techniques for Korean traditional patterns. T-shirts are a representative casual wear item worn in everyday life, especially among young people. The association of traditional elements and public goods such as T-shirts effects the diffusion of the Korean image into global society. Through the whole design process, target groups and design concepts were determined first: collegiate students as well as clean, natural, refined, simple and sporty image, respectively. Subsequently, Dokkaebi, lotus, Dangcho, dragon and Taegeuk patterns were selected as traditional pattern sources. Along with the technique of the division, transformation, and configuration of original patterns, the combining method of geometrical figures, stripe patterns, and check patterns with traditional patterns was helped create modernized traditional motives. Finally, T-shirt designs with a Korean image were developed with the harmonized deployment of modernized motives and color planning. The survey showed that designs had a clean, natural, refined, simple and sporty image that was preferred by collegiate students. This study is relevant in three aspects. First, the design process of modernization was very creative to combine traditional patterns and basic figures. Second, this study expanded the scope of traditional image design to casual wear to promote the Korean image to young people all over the world. Third, the whole design process, from planning to final product development, presented in this study is applicable to other design processes.

• Journal of Plant Biotechnology
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• 제38권1호
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• pp.9-14
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• 2011

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate cultivars metabolically, leaves of nine commercial Orostachys plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Partial least square discriminant analysis (PLS-DA). The dendrogram based on hierarchical clustering analysis of these PLS-DA data separated the nine Orostachys species into five major groups. The first group consisted of O. iwarenge 'Yimge', 'Jeju', 'Jeongsun' and O. margaritifolius 'Jinju' whereas in the second group, 'Sacheon' was clustered with 'Busan,' both of which belong to O. malacophylla species. However, 'Samchuk', belong to O. malacophylla was not clustered with the other O. malacophylla species. In addition, O. minuta and O. japonica were separated to the other Orostachys plants. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from leaves represented the most probable chemotaxonomical relationship between commercial Orostachys plants. Furthermore these metabolic discrimination systems could be applied for reestablishment of precise taxonomic classification of commercial Orostachys plants.

• 대한임상검사과학회지
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• 제39권1호
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• pp.1-6
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• 2007

Cervical cancer is one of the most prevalent cancers developed in women worldwide, and human papillomavirus(HPV) type 16 is the most common agent linked to human cerivical carcinoma. Viral oncogenes E6 and E7 are selectively retained and expressed in carcinoma cells infected with human papillomavirus type 16 and cooperate with each other in the immortalization and transformation of primary keratinocytes. Because the HPV oncogenesis mechanism was not completely solved, more thorough studies are required. ln the present study, we investigated the telomere independent role of telomerase in HPV oncogenesis, we constructed the E6 mutant, E7, E6/E7 and hTERT over-expressed stable cells with a telomerase negative cell line, SW13. Expressions of inserted genes were measured by RT-PCR. E6, E7 and hTERT genes were well expressed in each cell lines when compared with the control groups. By analyzing the cell morphology under the microscope, hTERT clone size was a smaller than the mock control but oncogene expressed clones had a slightly lengthened marginal region. In addition, hTERT cells also has a tendency of brief dividing time compared to the mock control. To determine whether telomerase activity was associated with a HPV oncogenesis by oncoprotein expression, we performed the PCR based TRAP assay and a Northern blot analysis. In TRAP assay data, telomerase activities in hTERT and oncogene clones increased compared to the mock control. In addition, SW13/E6/E7 cells showed an extremely increased activity compared to the other clones. Induced hTERT mRNA by E6/E7 wasn't, however, detected in Northern blotting. In conclusion, these findings suggest that telomerase activity is closely associated with the HPV oncogenesis and E6/E7 co-expression is a most important factor of telomerase activity.

• 한국재료학회지
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• 제28권7호
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• pp.381-390
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• 2018

Shape memory alloys(SMAs) have revolutionized the material engineering sciences as they exhibit exclusive features i.e. shape memory effect(SME) and super-elasticity. SMAs are those alloys that when deform return to their predeformed shape upon heating, they also restore their original shape by removing the load. Research on properties of newly advent of several types of ferrous based shape memory alloys(Fe-SMAs), shows that they have immense potential to be the counterpart of Nitinol(NiTi-SMA). These Fe-SMAs have been used and found to be effective because of their low cost, high cold workability, good weldability & excellent characteristics comparing with Nitinol(high processing cost and low cold workability) SMAs. Some of the Fe-SMAs show super-elasticity. Fe-SMAs, especially Fe-Mn-Si alloys have an immense potential for civil engineering structures because of its unique properties e.g. two-way shape memory effect, super elasticity and shape memory effect as well as due to its low cost, high elastic stiffness and wide transformation hysteresis comparative to Nitinol. Further research is being conducted on SMAs to improve and impinge better attributes by improving the material compositions, quantifying the SMA phase transition temperature etc. In this research pre-existing Fe-SMAs are categorised and collected in a tabulated form. An analysis is performed that which category is mostly available. Last 50 years data of Fe-SMA publications and US Patents is collected to show its importance in terms of increasing research on such type of alloys to invent different compositions and applications. This data is analysed as per different year groups during last 50 years and it was analysed as per whether the keywords exist in title of an article or anywhere in the article. It was found that different keywords related to Fe-SMAs/categories of Fe-SMAs, almost don't exist in the title of articles. However, these keywords related to Fe-SMAs/categories of Fe-SMAs, exist inside the article but still there are not too many publications related to Fe-SMAs/categories of Fe-SMAs.

• 한국환경교육학회지:환경교육
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• 제22권3호
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• pp.15-30
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• 2009

This study focuses on the comparison of PBL and GBS. PBL is a famous model that emphasizes diversity of students and self directed learning within the theory of constructivism. GBS has not been studied that extensively. GBS is most similar to PBL but this model has not been actively applied to school education. This study focuses on how to enhance students' interest in an educational environment that is encompassing a new and changing teach-learn paradigm and the development of new technology. Furthermore, PBL and GBS methods were compare actively applied to "Environmental Education" to make the students learn self-directed education through the transformation of one's environmental awareness. Specifically, the models were applied to the "Ecology and Environment" subject taught in general high schools and the degree of 'environmental literacy' were measured, From March to May of 2007, the PBL and GBS models were applied to 3rd grade students at D high school and the subject was 'Ecology and Environment'. The students were divided according to age into groups of $5{\sim}7$. The PBL group attempted to solve the given problems and the GBS group carried out their missions. In conclusion, after analyzing the 'environmental literacy' survey results by SPSS 12.0, just 'Knowledge of Ecology (KEOC)' and 'Locus of Control (LOC)' were slightly significant in PBL. This shows that PBL enhances the students' problem solving ability and thinking power. This is a result of the enhancement of students' problem-solving and thinking abilities through PBL. Students can learn many things because they completed their projects based on their experience and knowledge, both individually and interpersonally. We have to study how to apply PBL and GBS to the education field in spite of the many limited conditions. Therefore, this study looks at the application of PBL and GBS in environmental education and suggests specific strategies for educators who want to apply GBS and PBL and adapt them into their education curricula.

• Journal of Ginseng Research
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• 제40권1호
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• pp.47-54
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• 2016

Background: The roots of Panax ginseng contain noble tetracyclic triterpenoid saponins derived from dammarenediol-II. Dammarene-type ginsenosides are classified into the protopanaxadiol (PPD) and protopanaxatriol (PPT) groups based on their triterpene aglycone structures. Two cytochrome P450 (CYP) genes (CYP716A47 and CYP716A53v2) are critical for the production of PPD and PPT aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes PPT production from PPD in P. ginseng. Methods: We constructed transgenic P. ginseng lines overexpressing or silencing (via RNA interference) the CYP716A53v2 gene and analyzed changes in their ginsenoside profiles. Result: Overexpression of CYP716A53v2 led to increased accumulation of CYP716A53v2 mRNA in all transgenic roots compared to nontransgenic roots. Conversely, silencing of CYP716A53v2 mRNA in RNAi transgenic roots resulted in reduced CYP716A53v2 transcription. HPLC analysis revealed that transgenic roots overexpressing CYP716A53v2 contained higher levels of PPT-group ginsenosides ($Rg_1$, Re, and Rf) but lower levels of PPD-group ginsenosides (Rb1, Rc, $Rb_2$, and Rd). By contrast, RNAi transgenic roots contained lower levels of PPT-group compounds and higher levels of PPD-group compounds. Conclusion: The production of PPD- and PPT-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic P. ginseng. The biological activities of PPD-group ginsenosides are known to differ from those of the PPT group. Thus, increasing or decreasing the levels of PPT-group ginsenosides in transgenic P. ginseng may yield new medicinal uses for transgenic P. ginseng.