Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Prediction and discrimination of taxonomic relationship within Orostachys species using FT-IR spectroscopy combined by multivariate analysis

FT-IR 스펙트럼 데이터의 다변량 통계분석 기법을 이용한 바위솔속 식물의 분류학적 유연관계 예측 및 판별

  • Kwon, Yong-Kook (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Suk-Weon (Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Seo, Jung-Min (Omicisis Co.) ;
  • Woo, Tae-Ha (Omicisis Co.) ;
  • Liu, Jang-Ryol (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 권용국 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김석원 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 서정민 ((주)오믹시스) ;
  • 우태하 ((주)오믹시스) ;
  • 유장렬 (한국생명공학연구원 식물시스템공학연구센터)
  • Received : 2011.03.04
  • Accepted : 2011.03.14
  • Published : 2011.03.31
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Abstract

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate cultivars metabolically, leaves of nine commercial Orostachys plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Partial least square discriminant analysis (PLS-DA). The dendrogram based on hierarchical clustering analysis of these PLS-DA data separated the nine Orostachys species into five major groups. The first group consisted of O. iwarenge 'Yimge', 'Jeju', 'Jeongsun' and O. margaritifolius 'Jinju' whereas in the second group, 'Sacheon' was clustered with 'Busan,' both of which belong to O. malacophylla species. However, 'Samchuk', belong to O. malacophylla was not clustered with the other O. malacophylla species. In addition, O. minuta and O. japonica were separated to the other Orostachys plants. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from leaves represented the most probable chemotaxonomical relationship between commercial Orostachys plants. Furthermore these metabolic discrimination systems could be applied for reestablishment of precise taxonomic classification of commercial Orostachys plants.

Keywords

References

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