• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.22-27
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• 1999

Bovine oocytes with compact and complete cumulus cells were cultured in 6 groups for up to 24h in TCM199 buffered with 25 mmol/1 HEPES and supplemented with 10% FCS, 1 mg/ml $17{\beta}$-estradiol, 20 IU/ml hCG. Half of the oocytes at each group cultured in the presence of $25{\mu}g/ml$ cycloheximide at different times during maturation (0, 6, 12, 18, 20, 22 h) were fixed at 24 h of maturation to examine the nuclear progression. The rests of them were inseminated with frozen-thawed spermatozoa in medium BO with 10 mg/ml BSA and 10 mg/ml heparin and fixed after additional 18-20 h culture to evaluate the sperm head transformation. When a protein synthesis inhibitor was added at the onset of the maturation, the oocytes were prevented to proceed GVBD. A few of the oocytes (16%) were able to be penetrated and sperm head decondensation was inhibited either. Addition of cycloheximide after 6-12 h of culture resulted in an increasing percentage of GVBCD (more than 80%), but the oocytes became arrested in M-I (69.2%). More than half of the oocytes was penetrated with a decondensing sperm head. Formation of male pronucleus was first obtained at 12 h of culture in the presence of cycloheximide. When cycloheximide was added from 18 h of culture onwards, nuclear progression to M-II was increasingly restored (80.4-85.5%). The proportion of male and female pronuclear formation increased from 17.9% to 46.2%. It is concluded that protein synthesis is necessary not only for GVBD and development from M-I to M-II, but also for sperm head decendensation and male pronuclear formation in bovine oocytes.

• 한국전문물리치료학회지
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• 제24권4호
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• pp.38-45
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• 2017

Background: To improve muscle flexibility, static stretch is the most common type and is considered safe and effective for improving overall flexibility of muscles. During the stretch, the intensity is more likely to be determined by the degree of an athlete's pain and practitioner's skills rather than quantitative measures of stretch. It is necessary to determine the optimal intensity for the stretch. Objects: The purpose of this study is to explore the relationship between hand held dynamometer (HHD) and verbal rating scale (VRS) in comparison of the effects of continuance time on active (walking) and inactive (sitting) movement after static stretch. Methods: A cross-sectional study was conducted with a sample (n=62) recruited from a university. Participants were randomly assigned to 2 different groups (n=31 for each group) based on participants' positions either remaining in sitting or freely walking around for a series of re-assessments. Data was collected at pre-warm up, pre-stretch, post-stretch, and additional assessments at the time of 3, 6, 9, 12, 15, 20 and 30 minutes after the stretch. Results: Relationship between VRS and HHD scores represents very weak correlation (Spearman's p=-.16, p>.05). Pearson's correlation analysis was conducted following the logarithmic transformation of the two scores. Pearson's correlation after the transformation still showed a very low relationship and a poor linear relationship between the two scores (Pearson's r=-.18, p>.05). Conclusion: The optimal intensity for stretch cannot be solely determined by the subjective pain perception. The objective measurement such as HHD could be used in conjunction with the pain perception.

• 감성과학
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• 제12권1호
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• pp.129-136
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• 2009

캐리커처 자동 생성기를 개발하기 위한 본 연구는 과거의 여러 연구와는 달리 캐리커처 전문가들이 그리는 방법을 관찰하는 것에서 시작하였다. 관찰 과정에서 전문가들이 특히 그림을 그릴 때 대상물의 형태를 다른 유사한 다른 사물과 비슷하게 그려서 그 특징을 과장하고 있는 사실을 발견하였다. 우리가 일상생활에서 '무엇은 무엇을 닮았다'라고 표현할 때, 이는 동일한 형태 군에서 그 차이점을 부각 시켜서 표현하는 것이다. 본 연구에서는 그리고자 하는 대상물의 형태와 가장 유사한 기하학적 형태를 찾아 그 형태를 강조 변화 하고자 하였다. 연구의 범위는 벡터라인으로 그려진 2차원 단일 외형 폐곡선을 캐리커처 형태로 강조 변화하는 것으로 한정하였다. 이를 위하여 4종류의 유사 기하학적 형태 데이터베이스의 구성방법, 대상 유사 기하학적 형태를 찾기 위한 탐색 방법, 탐색된 데이터를 동일 크기와 좌표로 재배열 방법 그리고 강조 변화하는 방법에 관하여 논하였다. 사람 얼굴을 실험 대상으로 선정하여 데이터베이스를 제작하였다. 변화된 결과물에 대해서 설문한 결과 그 가능성이 입증되었다.

• 복식
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• 제59권8호
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• pp.82-96
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• 2009

The purpose of this study is to present the basic approach of producing the luxury fashion brands containing of the Korean traditional traits on the basis of traditional transformation with the modern concept, taking the 'Vivienne Tam' 2000's collections. This study has focused on its transformation of the traditional one of China, and made this as the subject of investigation. It's design characteristic could be defined as "modern interpretations of China chic", and it would be divided into two groups. The ingenious mixture of Chinese tradition into the modern chic could be concluded like these. The external characteristics is categorized in the three ways (1) the aesthetic application of the Chinese traditional patterns(dragon, water waves, peony, Japanese apricot flower, bamboo, bats, Chinese characters etc.), (2)the modern application of Chinese traditional costume details(front opening of Chipao, mandarin collar and knot buttons) and (3)the modernization of Chinese traditional technique(knotting, embroidery, beading and paper cutting). To deal with the internal characteristics, (1)the aesthetic mixture of East and West, (2)the formative expressions of the traditional view on the universe and religion are remarkable. The Chinese embodiment and the view of the universe and religion was integrated into the patterns of dragon, water waves, clouds, fire, woods, and metals. In order to afford the creative designer capable of encompassing the East and West, the teaching about the Korean tradition along with the technical and practical aspect of fashion is most important, while encouraging the professional designer to make a sophisticated ones which are attributed to the Korean tradition, and thereby come to be attractive to the world customer. The study about the Korean costumes, traditional colors, the symbolic meaning of the traditional patterns, cuttings, compositions, extending to the various kinds of myths, songs, paintings and crafts are essential for the Korean designer brand to be the global luxury brands.

• 시큐리티연구
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• 제37호
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• pp.83-108
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• 2013

최근 들어 테러단체들의 범죄활동을 소위 "테러와 범죄와의 결합현상(the Crime-Terror Nexus)" 라고 명명하고 있다. 이 현상은 테러집단들이 새로운 변화에 발맞추어 진화되고 있는 테러리즘의 새 패러다임을 일컫는 개념이다. 즉, 서로 다른 배타적인 성격과 영역에 있다고 여겨졌던, 범죄 집단과 테러집단이 연합하거나 또는 두 집단이 하나의 집단의 속성을 띄도록 서로 수렴되고 있는 전이(transformation)현상을 나타내는 것으로 이해되고 있다. 이와 같은 현상이 주목받아야 할 이유는 두 집단의 속성이 서로 합쳐져 하나의 시너지 효과를 내면서 이전의 테러활동의 수준을 벗어난 국제질서와 안보의 큰 위협이 되고 있기 때문이다. 이 연구는 중동과 아프리카지역의 테러단체에 대한 정보를 담고 있는 START center와 Minority at Risk 프로젝트의 MAROB(the Minorities at Risk Organizational Behavior) 데이터를 사용하여 테러의 새로운 진화 현상에 영향을 미치는 요인들을 탐색적으로 분석하기 위한 경험적 연구를 수행하였다. 테러와 범죄의 결합현상이 가까운 미래에 가져올 위협과 문제점들이 있으나 이에 대한 연구의 부족과, 테러에 관련된 경험적 연구의 수행의 어려움 등을 고려해 볼 때, 이 연구는 테러리즘에 관련된 학문적 영역 모두에서 매우 큰 공헌점이 있다고 할 것이다.

• 한국콘텐츠학회논문지
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• 제10권6호
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• pp.352-359
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• 2010

본 연구에서는 전기성형법을 이용한 금속-도재관의치의 도재소성에 따른 물성변화와 변연간격을 측정하여 변연 적합도에 미치는 영향을 고찰하여 정밀성을 평가하고자 한다. 물성변화와 변연간격을 관찰하기 위하여 물성 관찰시편은 소성 횟수를 고려하여 소성을 0회(HC), 1회(H1), 2회(H2), 3회(H3) 실시한 군으로 분류하였으며, 변연간격 관찰시편은 전용결합재 처리 군(FS), 전용결합재와 opaque 처리 군(FO), 전용 결합재와 opaque와 bady를 처리한 군(FB) 그리고 소성이 없는 군(FC)으로 분류하였다. 물성 관찰시편의 미소경도 관찰결과 대조군(HC)에 비하여 시험군(H1, H2, H3)의 경도가 감소하는 것을 관찰 할 수 있었다(p<0.05). 그리고 변연간격 관찰결과 GES 금속-도재관의 제작과정 중 발생하는 도재의 소성 횟수가 변연부에는 영향을 주지 않는 것을 관찰할 수 있었다(p>0.05).본 연구를 종합하여 분석한 결과 소성과정에서 열에 의한 연화열처리 효과로 경도감소가 발생하지만 변연간격에는 영향을 미치지 않은 결과를 얻어 GES 금속-도재관의 제작과정에서 도재소성으로 인한 변연 적합도 변화는 없으며 정밀도에도 영향을 미치지 않는 것으로 판단된다.

• 한국초등수학교육학회지
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• 제18권1호
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• pp.1-17
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• 2014

초등학교에서 곱셈의 교환법칙의 지도는 $3{\times}4=12$, $4{\times}3=12$와 같이 $a{\times}b$와 $b{\times}a$ 의 값을 계산하고 실제로 그 값이 같은지를 확인하는 활동을 바탕으로 하는 것이 보통이다. 이 논문에서는 첫째로, 순수이성비판에 나타난 수학적 지식에 관한 칸트의 견해를 바탕으로, 곱셈의 교환법칙의 취급 방법을 비판적으로 고찰한다. 칸트에 의하면, 수학적 지식은 선험성과 도식성이라는 특징을 지니고 있다. 두 곱셈의 계산 결과를 비교하는 방법은 선험성과 도식성이라는 수학적 지식의 특성을 충족하지 못한다. 칸트의 관점에서 볼 때, $a{\times}b$를 $b{\times}a$ 로 변환하는 필연적이고 일반적인 도식이 드러나게 교환법칙을 취급하는 것이 적절하다. 둘째로, 곱셈의 교환법칙의 도식과 관련된 기본구성단위로의 분배 전략은 (자연수)${\times}$(10의 거듭제곱), 몫 분수 맥락에서 분수의 복합적 의미, 분수의 곱셈과 같은 학습 내용을 관통하는 일반적인 성격의 것임을 논한다. 끝으로, 이상의 두 논의를 바탕으로 초등 수학교과서에서 곱셈의 교환법칙이 다루어지는 방식을 비판적으로 고찰한다.

• 대한한의학방제학회지
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• 제5권1호
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• pp.184-202
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• 1997

The results of immune response and antiallergic reaction were as follows. 1. Hemagglutinin titer and hemolysin titer were increased in case of AMR, AR, SNH. But the results were not recognized as having significance. 2. PFC was increased in case of AMR, AR. But the results were not recognized as having significance. 3. RFC was increased in all groups, and the results in the AR, SNH were significant. 4. In experimentation of phagocytic activity in peritoneal exudate cells, AR and SNH showed significant increase. In spleen cells AR and SNH showed significant increase. In monolymphocytus cells AMR, AR and SNH were increased, but result of AMR was of no significance. 5. I examined promotion on spleen cells transformation. As these results, AMR showed increase in $50{\mu}g/m{\ell}$, $5000{\mu}g/m{\ell}$ in comparison with control group. And in $500{\mu}g/m{\ell}$ AMR showed increase in case of 24 hours, 72 hours incubation, but showed decrease in case of 48 hours incubation. AR showed increase in all. In $50{\mu}g/m{\ell}$ SNH showed increase in comparison with control group. And in $500{\mu}g/m{\ell}$, $5000{\mu}g/m{\ell}$ SNH showed increase in case of 24 hours, 48 hours incubation, but showed decrease in case of 72 hours incubation. 6. I examined proliferation of spleen cells. As these results AMR and SNH showed the highest increase in $50{\mu}g/m{\ell}$, but showed the lowest increase in $5000{\mu}g/m{\ell}$. AR showed the highest increase in $500{\mu}g/m{\ell}$, but this result was the almost same in $50{\mu}g/m{\ell}$, $5000{\mu}g/m{\ell}$. And AMR, AR, SNH showed higher activity in Lipopolysaccharide than Concanavalin A. 7. In all groups results of PCA were decreased in 2 week. In 4 week AR and SNH showed decrease, but AMR didn't show change. In 6 week AR and SNH showed decrease, but on the contrary AMR showed increase. 8. In experimentation on histamine contents, AMR showed significant increase at first agent contact. And AR, SNH showed decrease at first agent contact, but these results were of no importance. At second agent contact AMR showed decrease, but was of no importance. AR, SNH showed significant decrease. At third agent contact, AMR showed significant increase. AR, SNH showed decrease, but these results were of no importance. From above these results, AR and SNH showed good effects on immunoreaction. And all the herb medicines in this examination showed good effects in promotion on spleen cells transformation and proliferation of spleen cells, especially activated B-cells. AR, SNH showed good effects on anti-allergic reaction, but AMR was almost inefficient. Accordingly I think that AR shall be used in disease bringing about a lowering of immunity, that is, AR shall be used in strengthening the body resistance. And I think that SNH shall be used in eliminating pathogenic factors with strengthening the body resistance. It is necessary to a deep study in future.

• 치과방사선
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• 제28권1호
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• pp.245-259
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• 1998

Cellular transforming genes have been identified in a number of different tumor cell lines and tumor types. A significant number of these oncogenes belong to the ras gene family. The ras gene family consists of three closely related genes:H-ras, K-ras and N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one of the three ras genes convert these genes into acute oncogenes. The presence of H-ras gene mutations has important prognostic implications in various tumors. Each genomic DNA was isolated from tumors induced by implantation with DMBA, or by treatment with DMBA -implantation/irradiation. When genome DNA was transfected into NIH 3T3 cells and investigated by two-step PCR-RFLP, the fOllowing results were concluded: 1. Transformation foci developed in two groups when the genome DNA of two experimental groups were transfected into NIH 3T3 cells. 2. Transformation efficiency was 0.01-0.02 foci/㎍DNA in the experimental group with the DMBA-implantation, 0.01-0.03 foci/㎍lgDNA in the experimental group with the DMBA-implantation/irradiation according to results of transfection assay. 3. When the point mutation of H-ras gene was investigated by a two-step PCR-RFLP, there was 13.9％ (5/36) in the experimental group with the DMBA implantation, 15.4 ％ (6/39) in the experimental group with the DMBA -implantation/irradiation. 4. The point mutation in codon 12 and 61 of H-ras was 5.6％(2/36) and 8.3％(3/36) in the experimental group with the DMBA implantation. 5. The point mutation in codon 12 and 61 of H-ras gene was 7.7％(3/39) in the experimental group with the DMBA -implantation/irradiation.

• Journal of Chest Surgery
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• 제44권6호
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• pp.406-412
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• 2011

Background: Development of thoracic aortic aneurysms and aortic dissections (TAAD) is attributed to unbearable wall tension superimposed on defective aortic wall integrity and impaired aortic repair mechanisms. Central to this repair mechanisms are well-balanced and adequately functional cellular components of the aortic wall, including endothelial cells, smooth muscle cells (SMCs), inflammatory cells, and adventitial fibroblasts. Adventitial fibroblasts naturally produce aortic extracellular matrix (ECM), and, when aortic wall is injured, they can be transformed into SMCs, which in turn are involved in aortic remodeling. We postulated the hypothesis that adventitial fibroblasts in patients with TAAD may have defects in ECM production and SMC transformation. Materials and Methods: Adventitial fibroblasts were procured from the adventitial layer of fresh aortic tissues of patients with TAAD (Group I) and of multi-organ donors (Group II), and 4-passage cell culture was performed prior to the experiment. To assess ECM production, cells were treated with TNF-${\alpha}$ (50 pM) and the expression of MMP-2/MMP-3 was analyzed using western blot technique. To assess SMC transformation capacity, cells were treated with TGF-${\beta}1$ and expression of SM ${\alpha}$-actin, SM-MHC, Ki-67 and SM calponin was evaluated using western blot technique. Fibroblasts were then treated with TGF-${\beta}1$ (10 pM) for up to 10 days with TGF-${\beta}1$ supplementation every 2 days, and the proportion of transformed SMC in the cell line was measured using immunofluorescence assay for fibroblast surface antigen every 2 days. Results: MMP-3 expression was significantly lower in group I than in group II. TGF-${\beta}1$-stimulated adventitial fibroblasts in group I expressed less SM ${\alpha}$-actin, SM-MHC, and Ki-67 than in group II. SM-calponin expression was not different between the two groups. Presence of fibroblast was observed on immunofluorescence assay after more than 6 days of TGF-${\beta}1$ treatment in group I, while most fibroblasts were transformed to SMC within 4 days in group II. Conclusion: ECM production and SMC transformation are compromised in adventitial fibroblasts from patients with TAAD. This result suggests that functional restoration of adventitial fibroblasts could well be a novel approach for the prevention and treatment of TAAD.