• 한국콘텐츠학회논문지
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• 제19권10호
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• pp.535-547
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• 2019

본 연구는 노인체육지도자의 변혁적 리더십이 조직성과와 직무열의 및 혁신행동에 미치는 영향을 알아보기 위하여 서울, 경기, 인천 지역의 스포츠센터와 노인체육센터 6곳의 노인체육지도자 236명을 대상으로 연구하여 다음과 같은 결론을 도출하였다. 첫째, 노인체육지도자 리더의 변혁적 리더십은 조직성과의 모든 하위요인에 유의한 영향을 미치는 것으로 나타났다. 둘째, 변혁적 리더십은 직무열의의 모든 하위요인에 유의한 영향을 미치는 것으로 나타났다. 셋째, 변혁적 리더십은 혁신행동에 유의한 영향을 미치는 것으로 나타났다. 따라서 본 연구의 결과, 리더의 변혁적 리더십은 조직원들의 조직성과와 직무열의 및 혁신행동을 높여주는 중요한 변수임을 확인하였고, 변혁적 리더십이 조직원의 근무환경에 긍정적인 영향을 미치는 효과가 있음을 입증했다는 점에서 큰 의의가 있다.

• The Journal of Advanced Prosthodontics
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• 제11권1호
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• pp.65-74
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• 2019

PURPOSE. To evaluate and compare the effect of different materials and techniques on the shear bond strength of veneering ceramic materials to zirconia. MATERIALS AND METHODS. 136 sintered zirconia cubes were prepared and randomly divided into four study groups according to corresponding methods of surface treatment and materials: GLN (grinding followed by laser scanning using Noritake Cerabien ZR), SLN (sandblasting followed by laser scanning using Noritake Cerabien ZR), GLV (grinding followed by laser scanning using VITA VM 9), and SLV (sandblasting followed by laser scanning using VITA VM 9). Spraying technique was performed to coat the core. Profilometer, SEM, XRD, EDS, universal testing machine, and stereomicroscope were used to record surface roughness Ra, surface morphology, phase transformation, elemental compositions, shear bond strength SBS values, and failure types, respectively. Specimens were investigated in unaged (not immersed in artificial saliva) and aged (stored in artificial saliva for a month) conditions to evaluate SBS values. RESULTS. Grinding and GLN as first and second surface treatments provided satisfactory Ra values in both conditions ($1.05{\pm}0.24{\mu}m$, $1.30{\pm}0.21{\mu}m$) compared to sandblasting and other groups (P<.05). The group GLN showed the highest SBS values in both conditions ($30.97{\pm}3.12MPa$, $29.09{\pm}4.17MPa$), while group SLV recorded the lowest ($23.96{\pm}3.60MPa$, $22.95{\pm}3.68Mpa$) (P<.05). Sandblasting showed phase transformation from t-m. Mixed failure type was the commonest among all groups. CONCLUSION. GLN showed to be a reliable method which provided satisfactory bond strength between the veneer ceramic and zirconia. This method might preserve the integrity of fixed dental crowns.

• 식물조직배양학회지
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• 제27권4호
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• pp.269-282
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• 2000

Benzylisoquinoline alkaloids are a diverse group of natural products that include many pharmacologically active compounds produced in a limited number of plant families. Despite their complexity, intensive biochemical research has extended our knowledge of the chemistry and enzymology of many important benzylisoquinoline alkaloid pathways, such as those leading to the analgesic drugs morphine and codeine, and the antibiotics sanguinarine and berberine. The use of cultured plant cells as an experimental system has facilitated the identification and characterization of more than 30 benzylisoquinoline alkaloid biosynthetic enzymes, and the molecular cloning of the genes that encode at least 8 of these enzymes. The recent expansion of biochemical and molecular technologies has creat-ed unique opportunities to dissect the mechanisms involved in the regulation of benzylisoquinoline alkaloid biosynthesis in plants. Research has suggested that product accumulation is controlled by the developmental and inducible regulation of several benzylisoquinoline alkaloid biosynthetic genes, and by the subcellular compartmentation of biosynthetic enzymes and the intracellular localization and trafficking of pathway intermediates. In this paper, we review our current understanding of the biochemistry, cell biology, and molecular regulation of benzylisoquinoline alkaloid biosynthesis in plants. We also summarize our own research activities, especially those related to the establishment of protocols for the genetic transformation of benzylisoquinoline alkaloid-producing species, and the development of metabolic engineering strategies in these plants.

• 한국분말재료학회지
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• 제13권4호
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• pp.243-249
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• 2006

Yttrium aluminum garnet (YAG) powders were synthesized via mechanochemical solid reaction using $Y_2O_3$ with three types of aluminum compounds. $Y_2O_3$ reacted mechanochemically with all A1 compounds and formed YAM (yttrium aluminum monoclinic), YAG and YAP (yttrium aluminum perovskite) phases depending on the starting materials. The ground samples containing ${\gamma}-A1_2O_3$ showed the best reactivity, whereas the ground sample containing A100H, which had the largest surface area, exhibited pure YAG after calcination at $1200^{\circ}C$. The sample containing Al had the least reactivity, producing YAP along with YAG at $1200^{\circ}C$. The types and grinding characteristics of the starting materials and grinding time are believed to be important factors in the mechanochemical synthesis of YAG.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 춘계 학술발표회
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• pp.457-467
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• 2010

The settlement prediction is very important to preloading method for a construction site on a soft ground. At the design stage, however, it is hard to predict the settlement exactly due to limitations of the site survey. Most of the settlement prediction is performed by a regression settlement curve based on the field data during a construction. In Korea, hyperbolic method has been most commonly used to align the settlement curve with the field data, because of its simplicity and many application cases. The results from hyperbolic method, however, may be differed by data selections or data fitting methods. In this study, the analyses using hyperbolic method were performed about the field data of $\bigcirc\bigcirc$ site in Pusan. Two data fitting methods, using an axis transformation or an alternative method, were applied with the various data group. If data was used only after the ground water level being stabilized, fitting results using both methods were in good agreement with the measured data. Without the information about the ground water level, the alternative method gives better results with the field data than the method using an axis transformation.

• Journal of Plant Biotechnology
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• 제35권1호
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• pp.41-45
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• 2008

Laccase (EC 1.10.3.2) is a small group of enzymes that catalyze the oxidation of a broad range of phenolic compounds including hazardous and recalcitrant pollutants in the environment. This study attempted to develop an efficient system for production of a recombinant laccase by chloroplast genetic transformation of tobacco. Chloroplast transformation vector was constructed and introduced into the tobacco chloroplast genome using particle bombardment. Chloroplast-transformed plants were subsequently regenerated. PCR and southern blot analyses confirmed stable integration of the laccase gene into the chloroplast genome. Northern blot analysis revealed that mRNA of the laccase gene was highly expressed in chloroplast-transformed plants.

• Molecules and Cells
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• 제21권3호
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• pp.436-442
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• 2006

Different proliferation of neuroblast 6-4 (NB6-4) in the thorax and abdomen produces segmental specific expression pattern of several neuroblast marker genes. NB6-4 is divided to form four medialmost cell body glia (MM-CBG) per segment in thorax and two MM-CBG per segment in abdomen. As homeotic genes determine the identities of embryonic segments along the A/P axis, we investigated if temporal and specific expression of homeotic genes affects MM-CBG patterns in thorax and abdomen. A Ubx loss-of-function mutation was found to hardly affect MM-CBG formation, whereas abd-A and Abd-B caused the transformation of abdominal MM-CBG to their thoracic counterparts. On the other hand, gain-of-function mutants of Ubx, abd-A and Abd-B genes reduced the number of thoracic MM-CBG, indicating that thoracic MM-CBG resembled abdominal MM-CBG. However, mutations in Polycomb group (PcG) genes, which are negative transregulators of homeotic genes, did not cause the thoracic to abdominal MM-CBG pattern transformation although the number of MM-CBG in a few percent of embryos were partially reduced or abnormally patterned. Our results indicate that temporal and spatial expression of the homeotic genes is important to determine segmental-specificity of NB6-4 daughter cells along the anterior-posterior (A/P) axis.

• Journal of Ginseng Research
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• 제38권4호
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• pp.264-269
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• 2014

Background: In this study, we examined the effects of various enzymes on chemical conversions of ginsenosides in ginseng extract prepared by amylases. Methods: Rapidase, Econase CE, Viscozyme, Ultraflo L, and Cytolase PCL5 were used for secondary enzymatic hydrolysis after amylase treatment of ginseng extract, and ginsenoside contents, skin permeability, and chemical compositions including total sugar, acidic polysaccharide, and polyphenols were determined on the hydrolyzed ginseng extract. Results: Rapidase treatment significantly elevated total ginsenoside contents compared with the control (p < 0.05). In particular, deglycosylated ginsenosides including Rg3, which are known as bioactive compounds, were significantly increased after Rapidase treatment (p < 0.05). The Rapidase-treated group also increased the skin permeability of polyphenols compared with the control, showing the highest level of total sugar content among the enzyme treatment groups. Conclusion: This result showed that Rapidase induced the conversion of ginsenoside glycosides to aglycones. Meanwhile, Cytolase PCL5 and Econase treatments led to a significant increase of uronic acid (acidic polysaccharide) level. Taken together, our data showed that the treatments of enzymes including Rapidase are useful for the conversion and increase of ginsenosides in ginseng extracts or products.

• Journal of Electrical Engineering and Technology
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• 제8권2호
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• pp.271-281
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• 2013

In order to improve the performance of analysis, it is important to consider the nonlinearity in power system. The Carleman embedding technique (linearization procedure) provides an effective approach in reduction of nonlinear systems. In the approach, a group of differential equations in which the state variables are formed by the original state variables and the vector monomials one can build with products of positive integer powers of them, is constructed. In traditional Carleman linearization technique, the tensor matrix is truncated to form a square matrix, and then regular linear system theory is used to solve the truncated system directly. However, it is found that part of nonlinear information is neglected when truncating the Carleman model. This paper proposes a new approach to solve the problem, by combining the Poincar$\acute{e}$ transformation with the Carleman linearization. Case studies are presented to verify the proposed method. Modal analysis shows that, with traditional Carleman linearization, the calculated contribution factors are not symmetrical, while such problems are avoided in the improved approach.

• Smart Structures and Systems
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• 제21권2호
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• pp.195-205
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• 2018

Research on Lamb wave-based damage identification in plate-like structures depends on precise knowledge of dispersive wave velocity. However, boundary reflections with the same frequency of interest and greater amplitude contaminate direct waves and thus compromise measurement of Lamb wave dispersion in different materials. In this study, non-reflecting boundaries were proposed in both numerical and experimental cases to facilitate time-frequency characterization of Lamb wave dispersion. First, the Lamb wave equations in isotropic and laminated materials were analytically solved. Second, the non-reflecting boundaries were used as a series of frames with gradually increased damping coefficients in finite element models to absorb waves at boundaries while avoiding wave reflections due to abrupt property changes of each frame. Third, damping clay was sealed at plate edges to reduce the boundary reflection in experimental test. Finally, the direct waves were subjected to the slant-stack and short-time Fourier transformations to calculate the dispersion curves of phase and group velocities, respectively. Both the numerical and experimental results suggest that the boundary reflections are effectively alleviated, and the dispersion curves generated by the time-frequency analysis are consistent with the analytical solutions, demonstrating that the combination of non-reflecting boundary and time-frequency analysis is a feasible and reliable scheme for characterizing Lamb wave dispersion in plate-like structures.