• Title/Summary/Keyword: transformation by-products

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Identification of Korean Ginseng Cytochrome P450 gene and Its Characterization by Transformation System (고려인삼 유래 Cytochrome P450 유전자의 동정 및 형질전환에 의한 특성검정)

  • Shim, Ju-Sun;Kim, Yu-Jin;Jung, Seok-Kyu;Kwon, Woo-Saeng;Kim, Se-Young;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.33 no.3
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    • pp.212-218
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    • 2009
  • Triterpenoid saponins were synthesized in Panax ginseng C.A. Meyer via the isoprenoid pathway by cyclization of 2,3-oxidosqualene to give primarily oleanane (beta-amyrin) or dammarane triterpenoid skeletons. The triterpenoids are backbone and undergoes various modifications (oxidation, substitution and glycosylation), mediated by cytochrome P450 (CYP)-dependent monooxygenases, glycosyltransferase and other enzymes. This is likely to be due in part to the complexity of the molecules and the lack of pathway intermediates for biochemical studies. A cDNA clone encoding a putative CYP gene was isolated from flower bud of ginseng and transformed into the plant(Nicotiana tabacum cv. Xanthi) and confirmed by PCR analysis. The CYP gene (PgCYP) contained an open reading frame(ORF) encoding mature protein of 500 amino acids. The expression of PgCYP were investigated in transgenic tobacco by reverse transcriptase-polymerase chain reaction (RT-PCR).

Quantitative aspects of the hydrolysis of ginseng saponins: Application in HPLC-MS analysis of herbal products

  • Abashev, Mikhail;Stekolshchikova, Elena;Stavrianidi, Andrey
    • Journal of Ginseng Research
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    • v.45 no.2
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    • pp.246-253
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    • 2021
  • Background: Ginseng is one of the most valuable herbal supplements. It is challenging to perform quality control of ginseng products due to the diversity of bioactive saponins in their composition. Acid or alkaline hydrolysis is often used for the structural elucidation of these saponins and sugars in their side chains. Complete transformation of the original ginsenosides into their aglycones during the hydrolysis is one of the ways to determine a total saponin group content. The main hurdle of this approach is the formation of various by-products that was reported by many authors. Methods: Separate HPLC assessment of the total protopanaxadiol, protopanaxatriol and ocotillol ginsenoside contents is a viable alternative to the determination of characteristic biomarkers of these saponin groups, such as ginsenoside Rf and pseudoginsenoside F11, which are commonly used for authentication of P. ginseng Meyer and P. quinquefolius L. samples respectively. Moreover, total ginsenoside content is an ideal aggregated parameter for standardization and quality control of ginseng-based medicines, because it can be directly applied for saponin dosage calculation. Results: Different hydrolysis conditions were tested to develop accurate quantification method for the elucidation of total ginsenoside contents in herbal products. Linearity, limits of quantification, limits of detection, accuracy and precision were evaluated for the developed HPLC-MS method. Conclusion: Alkaline hydrolysis results in fewer by-products than sugar elimination in acidic conditions. An equimolar response, as a key parameter for quantification, was established for several major ginsenosides. The developed approach has shown acceptable results in the analysis of several different herbal products.

A Supply Chain Management Process Modeling for an Agriculture Marketing Information System (농산물 유통 정보화를 위한 공급사슬경영 프로세스 모델링)

  • Myung, Kwang-Sick;Park, Sei-Kwon;Kang, Dae-Won
    • IE interfaces
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    • v.13 no.3
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    • pp.358-377
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    • 2000
  • A supply chain is a network of facilities and distribution options that performs the functions of procurement of materials, transformation of these materials into intermediate and finished products, and the distribution of these finished products to customers. SCM(Supp1y Chain Management), by definition, encompasses all activities associated with moving goods, from the raw materials stage through to the end user. It includes source and procurement, production scheduling, order processing, inventory management, transportation, warehousing, and customer service. Importantly, it encompasses the information systems used to monitor these activities. In this paper, the present situation and problems of marketing process in Korean agricultural environments were reviewed through a systematic methodology, and then we proposed a new business process for solving these problems by appling a supply chain management. We expect this supply chain management system applied to agricultural marketing process can improve significantly the rationality and transparency of Korean agricultural marketing structure.

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A Study on Design Development of Wood Products Applied a Concept of Hybrid (이종(異種 : Hybrid) 개념을 적용한 목제품(木製品) 디자인 개발에 관한 연구)

  • Yoon, Yeoh-Hang;We, Jin-Seok
    • Journal of the Korea Furniture Society
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    • v.24 no.4
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    • pp.318-328
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    • 2013
  • This study aimed to examine the theoretical consideration and concept of hybrid that could be applicable to wood product design, to analyze design trend according to the morphological transformation by means of combination of hybrid material and function, to understand the correlation between type of wood product and materials and to apply it to the domestic and overseas hybrid concept of wood product design study. A study of assembling output by empirical experiment to embed natural resources into acrylic resin in order to develop wood products was carried out. And an wood product was suggested that satisfied lighting and hanger function through a new wood product design development combined with hanger function and lighting function. Through such process, a new design paradigm was suggested to cope with the periodic trend and to induce mass production of designed product. Thus, this study set a new guideline for activating wood product design and attempted to meet the social demand of encouraging new value creation.

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Mediator-Assisted Biobleaching of Kraft Pulp by Laccase from Botrytis cinerea

  • Kim, Myungkil
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.3
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    • pp.52-58
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    • 2004
  • The use of N-hydroxyphthalimide (NHPI) as a mediator for laccase has proven to be comparable to N-hydroxybenzotriazole (HBT) for the delignification of kraft pulp, and the transformation of a number of industrial dyes. The advantages of NHPI derivatives are the biodegradation of these compounds compared to HBT, which has been shown to be recalcitrant in the environment, and the more reasonable cost of synthetic process.

The Effect of K-beauty SNS Influencer on Chinese Consumers' Acceptance Intention of New Products: Focused on Elaboration Likelihood Model (ELM) (K-beauty SNS 인플루언서가 중국 소비자의 신제품 수용의도에 미치는 영향 -정교화 가능성 모델(ELM)을 중심으로-)

  • Wang, Lei;Lee, Jin Hwa
    • Fashion & Textile Research Journal
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    • v.21 no.5
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    • pp.574-585
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    • 2019
  • The acceleration of digital transformation (DX) has resulted in SNS influencer marketing trends becoming the mainstream of the new market. SNS influencers act as early adopters in the process of new products being accepted. Chinese consumers are most affected by Hallyu, which increases interest in K-beauty products. This study analyzes how K-beauty SNS influencers are related to Chinese consumers. A survey was conducted among Chinese millennial consumers after watching videos provided by K-beauty SNS influencer; subsequently, 456 responses were used for data analysis. As a result, the analysis based on the Elaboration Likelihood Model (ELM) distinguishes the central route and the peripheral route in the process of Chinese consumers accepting new SNS products. The study findings suggested that information quality, credibility, accuracy, and usefulness had significant effects on acceptance intention for new products among central route factors, and similarity, trustworthiness, pleasure, expertise, and attractiveness also had significant effects on the acceptance intention of new product among peripheral route factors. It was found that variables of the central route, rather than those of peripheral route, had stronger effects on the acceptance intention for new products. Consequently, the central route of K-beauty SNS influencer is more important to Chinese consumers' acceptance of new products. It is expected that this study will offer beauty influencer marketing-based cosmetics brands efficient consumer management suggestions.

Effect of Humidity on Polymorphic Transformation of Hydrous Aluminum Oxide (알루미나수화물(水和物)의 결정전이(結晶轉移)에 미치는 습도(濕度)의 영향(影響))

  • Rhee, Gye-Ju;Yoo, Byeong-Tae
    • Journal of Pharmaceutical Investigation
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    • v.13 no.1
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    • pp.1-9
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    • 1983
  • The effect of humidity on crystallization and polymorphic transformation of hydrous aluminum oxide under various humidity at $37^{\circ}$ was examined by means of X-ray diffraction, scanning electron micrograph, IR spectra and DTA. The humidity was an important factor influencing crystallization of hydrous aluminum oxide. The growth or crystal was strongly accelerated by humidity. The aging process is assumed that it is composed of two seperate steps, an increase of the diffraction around $36{\sim}42^{\circ}$, and an appearance and its development of the peak at $18{\sim}20^{\circ}$ of $2{\theta}$ value. The former is considered to be nucleation and the latter correspond to the growth period on crystallization. The crystalline form of aging products was various depending on the degree of humidity, directly it leads to the eventual formation of bayerite in more than 72%, $b{\"{o}}hmite$ in 50% and resembled to Nordstandite in 0% relative humidity, respectively but once formed, it was mostly stable in each surroundings and does not transform to the other more stable form in solid state even after aging for five years. The mechanism responsible for aging is further polymerization process of six-membered rings by deprotonation-dehydration reaction in which positively charged polynuclear hydroxy aluminum complexes formed in the presence of moisture are joined at their edges by double hydroxide bridges.

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A Study on the Ocean Safety According to the Geodetic Datum Transformation (측지계변환에 따른 해양안전에 관한 연구)

  • 고광섭;임정빈;임봉택
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.1 no.2
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    • pp.39-52
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    • 1995
  • There are numerous mapping, charting, geodetic systems and electronic digital products defined in various local geodetic datum. It becomes a straight forward requirement to simplify the complexity by referencing all the products to a common reference globally. WGS-84 is well known as a state-of-the-art global reference system based on the use of data, techniques and technology available within American Defence Mapping Agency(DMA). Its parameters can be translated into more accurate maps, charts and geodetic positioning compared to others previously. Since Global Positioning System(GPS/NAVSTAR), which is asssociated with World Geodetic System(WGS-84)in reference frame, has been widely used, the unified geodetic system has been required for GPS users in many fields.

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Chloroplast Genetic Transformation in Higher Plants: An Encounter Between Prokaryote and Eukaryote (고등식물의 엽록체 형질전환: 원핵생물과 진맥생물의 조우)

  • Chung, Hwa-Jee;Suh, Young-Bae;Jeong, Won-Joong;Min, Sung-Ran;Liu, Jang-R.
    • Journal of Plant Biotechnology
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    • v.33 no.3
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    • pp.185-194
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    • 2006
  • Chloroplasts are believed to be descended from certain cyanobacteria, which were taken up by phagocytosis into a host cell and lived there in a symbiotic relationship. In contrast to the current static concept on the chloroplast genome, its dynamism has been recently demonstrated: the chloroplast genome is active in intramolecular homolgous recombination, producing subgenomic circles when it obtains homolgous sequences via genetic transformation. Chloroplast tranformation in higher plants provides many advantages over nuclear transformation that include higher expression levels of transgenes, polycistronic expression of transgenes, and maternal transmission of transgenes. Tobacco has been used as a model for chloroplast genetic transformation. However, it is recently possible to transform the chloroplasts of other major food and economic crops including rice, soybean, and cotton. Chloroplast-transformed crops will be able to replace bioreactors using microorganisms for production of value-added proteins in future.

Characteristics of Application of Augmented Reality Technology according to the Digital Transformation in the Fashion Industry (패션 산업의 디지털 전환에 따른 증강현실 기술의 활용특성)

  • Shin, Haekyung
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.1
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    • pp.597-603
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    • 2022
  • With the continuation of the pandemic environment, digital transformation is being applied in various fields of the fashion industry. Augmented reality technology is a form of overlapping virtual images on the real world, and as online shopping expands in non-face-to-face environments, the use of augmented reality technology in fashion and beauty fields is affecting consumer satisfaction and sales growth. In this study, the characteristics of augmented reality contents used in the fashion industry following digital transformation were extended to the fields of clothing, accessories, virtual fashion stores, and AR fashion shows to analyze the case characteristics of augmented reality. Augmented reality technology in the fashion industry focuses on promoting clothing and accessory products through SNS or homepage through marketing activities. It is raising positive values such as raising awareness. The range of types of augmented reality used in the future digital fashion environment will continue to expand, and by deriving the usage characteristics of augmented reality technology, it is intended to contribute to presenting a future vision of the fashion industry.