• Journal of Plant Biotechnology
• /
• v.35 no.2
• /
• pp.101-108
• /
• 2008

The Agrobacterium-mediated transformation has been successfully used method to introduce foreign genes into some monocotyledonous as well as a large number of dicotyledonous plants genome, We developed transgenic Chinese cabbage plants with insect-resistance gene, modified CryIAc, by Agrobacterium-transformation and confirmed transgene copy number by Southern blot analysis. We confirmed that twenty-nine out of 46 transgenic Chinese cabbage plants have single copy of CryIAc. To obtain the sequences information on the transferred DNA (T-DNA) integration into plant genome, we analyzed left border (LB) flanking sequences by genome walking (GW) PCR method. Out of 46 transgenic Chinese cabbage plants examined, 37 carried the vector backbone sequences. This result indicates that the transfer of the vector backbone from the binary vectors resulted mainly from inefficient termination of LB site. Analysis of T-DNA LB flanking region of 9 transgenic Chinese cabbage plants without vector backbone revealed that all LB ends were not conserved and nucleotides up to 36bp from the LB cleavage site were deleted.

• Geophysics and Geophysical Exploration
• /
• v.23 no.3
• /
• pp.192-207
• /
• 2020

We acquire seismic data with regularly or irregularly missing traces, due to economic, environmental, and mechanical problems. Since these missing data adversely affect the results of seismic data processing and analysis, we need to reconstruct the missing data before subsequent processing. However, there are economic and temporal burdens to conducting further exploration and reconstructing missing parts. Many researchers have been studying interpolation methods to accurately reconstruct missing data. Recently, various machine learning technologies such as support vector regression, autoencoder, U-Net, ResNet, and generative adversarial network (GAN) have been applied in seismic data interpolation. In this study, by reviewing these studies, we found that not only neural network models, but also support vector regression models that have relatively simple structures can interpolate missing parts of seismic data effectively. We expect that future research can improve the interpolation performance of these machine learning models by using open-source field data, data augmentation, transfer learning, and regularization based on conventional interpolation technologies.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.3
• /
• pp.355-362
• /
• 2000

The Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene in the pHLA-21 plasmid was inserted into a baculovirus (Hyphantria cunea nuclear polyhedrosis virus) expression vector (lacZ-HcNPV) to construct a recombinant virus gB-HcNPV expressing gB. Spodoptera frugiperda cells infected with this recombinant virus synthesized and processed gB of approximately 120 kDa, which cross-reacted with the monoclonal antibody to gB. The recombinant gB was identified on the membrane of the insect cells using an immunofluorescence assay. Antibodies to this recombinant raised in mice recognize the viral gB and neutralized the infectivity of the HSV-1 in vitro. These results show that the gB gene has the potential to be expressed in insect cells. They also demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the lacZ-HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV. Accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

• Journal of Plant Biotechnology
• /
• v.7 no.4
• /
• pp.233-240
• /
• 2005

Slices of embryonic axis of mature pea (Pisum sativum L. cv. Green Arrow) seeds were used as explant. Transformation of explants was done via Agrobacterium tumefaciens bearing vector pBI-P5CS construct. The best results for inoculation of explants were obtained when they were immersed for 90 s at a concentration of $6{\times}10^8$ cell $ml^(-1)$ of bacterial suspension. Transformed pea plants were selected on $50\;mg\;l^(-1)$ kanamycin and successful transformants were confirmed by PCR and blotting. Transgenic plants were further analyzed with RT-PCR to confirm the expression of P5CS. Transgenic plants and non-transgenic plants were treated with different concentrations of NaCl 0 (control), 100, 150 and 200 mM in culture medium. Measurement of proline content indicated that transgenic plants produced more amino acid proline in response to salt in comparison with non-transgenic plants. Photosynthetic efficiency in transgenic plants under salt-stress was more than that of non-transgenic plants.

• Journal of Electrical Engineering and Technology
• /
• v.11 no.6
• /
• pp.1769-1776
• /
• 2016

For on-line partial discharge (PD) monitoring of rotating machines, a novel sensor is proposed, which can be installed on the power lead inside the terminal box of the machine. The sensor has been designed to have high capacitance, and minimal reflection of measured pulses. As a sensitivity of the sensor, transfer impedance $Z_t$ has been measured and compared to conventional coupler-type sensors. A simple method is presented for measuring $Z_t$ of coupler sensors, using a vector network analyzer and a practical lead-cable of rotating machine. Through this method, it became possible to measure the $Z_t$ of coupler sensors including the installation environment of them. The $Z_t$ of the proposed sensor is higher than that of same sized other conventional couplers at frequencies between 30 and 92 MHz. Another sensitivity test has been performed using a PD calibrator as a test pulse source. The proposed sensor has higher measured peak voltage than the conventional coupler type sensors when the same charges were input.

• Journal of the Korean Society of Visualization
• /
• v.1 no.2
• /
• pp.29-37
• /
• 2003

During the past five decades or so, the characteristics of turbulent swirling flow have been studied extensively because of its great technological and scientific importance. It is well known that the swirling flow improves heat transfer in duct flow. The reason for this is due to the effect of streamline curvature associated with the tangential velocity component. Although many studies have been carried out to investigate the characteristics of the swirling flow in a circular tube. The experimental methods for measuring the velocity components are by hot-wire or LDV (Laser-Doppler-Velocimetry) measuring single point velocity so far. The present study was aimed to analyse the flow characteristics of swirling flow such as time-mean velocity vector, local velocity turbulence intensity and turbulence kinetic energy by using PIV(Particle-Image Velocimetry). The experiment was carried out for four Reynold numbers $1.0\times10^{4}$, $1.5\times10^{4}$, $2.0\times10^{4}$ and $2.5\times10^{4}$ of the measuring area.

• Journal of Electrical Engineering and Technology
• /
• v.12 no.6
• /
• pp.2118-2126
• /
• 2017

The large-scale power system blackouts have indicated that conventional protection relays that based on local signals cannot fit for modern power grids with complicated setting or heavily loaded-flow transfer. In order to accurately detect various faulted lines and improve the fault-tolerance of wide-area protection, a novel multi-section weighed fault matching and detecting algorithm is proposed. The real protection vector (RPV) and expected section protection vectors (ESPVs) for five fault sections are constructed respectively. The function of multi-section weighed fault matching is established to calculate the section fault matching degrees between RPV and five ESPVs. Then the fault degree of protected line based on five section fault degrees can be obtained. Two fault detecting criterions are given to support the higher accuracy rate of detecting fault. With the enumerating method, the simulation tests illustrate the correctness and fault-tolerance of proposed algorithm. It can reach the target of 100% accuracy rate under 5 bits error of wide-area protections. The influence factors of fault-tolerance are analyzed, which include the choosing of wide-area protections, as well as the topological structures of power grid and fault threshold.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.25 no.2
• /
• pp.187-193
• /
• 2012

Serine proteases are major insect enzymes involved in the digestion of dietary proteins and in the process of blood meal digestion. In this study, cDNA was constructed using the whole body of Laccotrephes japonensis. The flanking sequences of the 5- and 3- end of this gene were characterized by RACE-PCR. Sequence analysis showed that this gene contained a 963-bp ORF encoding 320 amino acids. The deduced amino acid sequence showed 62% identity with the Creontiades dilutus serine protease, 58% with the Lygus lineolaris trypsin precursor, and 54% with the Triatoma infestans salivary trypsin. To assess the expression of the L. japonensis serine protease (JGsp), the JGsp gene was cloned into a baculovirus transfer vector, pBac-1, and expressed in Sf9 cells (Spodoptera frugiperda). SDS-PAGE and western blot analysis have shown that the JGsp recombinant protein was a monomer with a molecular weight of about 32 kDa. Recombinant JGsp has shown activity in the protease enzyme assay using gelatin as a substrate.

• Journal of Microbiology and Biotechnology
• /
• v.22 no.8
• /
• pp.1059-1065
• /
• 2012

Biocatalytic transfer of oxygen in isolated cytochrome P450 or whole microbial cells is an elegant and efficient way to achieve selective hydroxylation. Cytochrome P450 CYP105P2 was isolated from Streptomyces peucetius that showed a high degree of amino acid identity with hydroxylases. Previously performed homology modeling, and subsequent docking of the model with flavone, displayed a reasonable docked structure. Therefore, in this study, in a pursuit to hydroxylate the flavone ring, CYP105P2 was co-expressed in a two-vector system with putidaredoxin reductase (camA) and putidaredoxin (camB) from Pseudomonas putida for efficient electron transport. HPLC analysis of the isolated product, together with LC-MS analysis, showed a monohydroxylated flavone, which was further established by subsequent ESI/MS-MS. A successful 10.35% yield was achieved with the whole-cell bioconversion reaction in Escherichia coli. We verified that CYP105P2 is a potential bacterial hydroxylase.

• Microbiology and Biotechnology Letters
• /
• v.18 no.6
• /
• pp.647-652
• /
• 1990

The production of delta-endotoxin crystal and the cloning of endotoxin protein gene in Bscillus thuringiensis subsp. kurstaki HD1 strain were studied. The strain produced bipyramidal crystals ($2.9\times 1.0 \mu m$) in their cells during sporulation. The B. thuringiensis contained about 10 plasmid DNA elements ranging from 2.1 to 80 kilobases. The 73 kb plasmid DNA, the 29 kb BamHI fragment and the 7.9 kb Pstl DNA fragment hybridized to the pHL probe. The 7.9 kb fragment was eluted and cloned in the PstI site of pBR322 vector and transformed into E. coli HB101, which produced insecticidal proteins killing Bornbyx mori larvae.