• Animal cells and systems
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• v.13 no.4
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• pp.411-418
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• 2009

We are reporting the identification, expression patterns, and possible biological functions of zebrafish kif3b (kif3bz) encoding 475 amino acids. Kif3Bz contains the kinesin motor domain, catalytic domain, KISc domain, and one single coiled coil domain. Phylogenetic analysis indicates that kif3bz is a highly conserved gene among the tested vertebrates. First of all, both maternal and zygotic messages of kif3bz were evenly distributed in the blastomeres at 2-cell stage. Its ubiquitous expression throughout the blastomeres continued till 40% epiboly. However, kif3bz transcripts became restricted in Kupffer's vesicle at tailbud and 6-somite stages. At 13-somite stage, kif3bz expression pattern became specific to the telencephalon, diencephalon, trigeminal placode, and somites. Such expression patterns were further intensified in the telencephalon, diencephalons, hind brain, pronephric ducts, optic vesicles, and spinal cord neurons in the 23-somite stage embryos, and last till 24 hpf. We discussed possible functions of Kif3Bz related to the vertebrate embryonic development.

• Journal of Plant Biology
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• v.37 no.3
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• pp.293-299
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• 1994

Developing rice endosperm cells display two morphologically distinct rough endoplasmic reticulum (ER) membranes, the cisternae ER (C-ER) and theprotein body ER (PB-ER), the latter delimiting the prolamine protein bodies. We (Li et al., 1993) have recently shown that the storage protein mRNAs are not randomly distributed on these ER types; the C-ER is enriched for glutelin mRNAs, whereas the PB-ER harbors predominantly prolamine transcripts. To address whether these ER types have differnet capacities to translate these mRNAs and translocate their proteins into the lumen, a microsomal fraction enriched in C-ER vesicles was prepared from devleoping rice seeds. When present in an in vitro translatin system, the microsomes were able to proteolytically remove the signal peptide and internalize both preproglutelin and preprolamine within the microsomal vesicles. Of the two species, preprolamine was more effectively translocated and processed. These results suggest that the C-ER has the capacity to recognize and bind both storage protein mRNAs during protein synthesis. Moreover, efficient translocation and processing of glutelin requires additional factors that are deficient or absent in the in vitro system.

• Molecules and Cells
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• v.20 no.2
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• pp.247-255
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• 2005

Three different catalase cDNA clones (CaCat1, CaCat2, and CaCat3) were isolated from hot pepper (Capsicum annuum L.), and their expression patterns were analyzed at the levels of mRNA and enzyme activity. Northern hybridization showed that the three catalase genes were differentially expressed in various organs, and that expression of CaCat1 and CaCat2 was regulated differently by the circadian rhythm. In situ hybridization revealed different spatial distributions of CaCat1 and CaCat2 transcripts in leaf and stem. In response to wounding and paraquat treatment, CaCat1 mRNA increased at 4-12 h in both paraquat-treated and systemic leaves. In contrast, wounding had no significant effect on expression of the catalase genes. The increase of catalase activity in the paraquat-treated and systemic leaves paralleled that of CaCat1 mRNA, but did not match that of CaCat1 mRNA in paraquat-treated stems. Our results suggest that CaCat1 may play a role in responses to environmental stresses.

• Journal of Applied Biological Chemistry
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• v.56 no.1
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• pp.37-42
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• 2013

In eukaryotes, most of the genome are transcribed, however only a small proportion of total transcripts encodes for protein, thus resulting in many of noncoding RNAs. In order to recover DNA damage including DNA double-strand breaks (DSBs) eukaryotes have evolved complex mechanisms and these are processed through coordinated mechanisms of protein sensors, transducers, and effectors including RNAs. During recent years, small RNAs have been increasingly studied and gradually considered as key regulators in various aspects of biology. Upon DNA damage, small RNAs including diRNAs (DSB induced RNA) are generated in both plant and human cell lines. Inhibition of their biogenesis has severe influence on DSB repair system.

• English Language & Literature Teaching
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• v.16 no.3
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• pp.119-139
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• 2010

The purpose of this paper was to examine various aspects of meaning negotiation process in online chatting. Korean college students were asked to engage in chatting on the Internet over the course of a semester-long period, and chatting transcripts were analyzed in terms of sources of communication breakdown, signals to indicate communication breakdown, strategies to overcome communication breakdown, and ways of closing meaning negotiation. According to the findings of the study, lack of background knowledge and incoherent string of sentences in text were two major barriers creating communication problems. Subjects were able to use signals to indicate their communication difficulties, and overcome them by using different strategies. In doing so, however, they were found to suffer a narrow range of signals and strategies, which showed their limited communicative ability in the management of interaction, and indicated a clear, strong need for an extension of discourse and strategic competences of Korean students for more effective and smoother transition of message in everyday interaction.

• Korean Journal of Child Studies
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• v.23 no.6
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• pp.1-14
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• 2002

This study investigated topic continuity in 5-year-old children during verbal interactions with peers by socioeconomic status and type of task. Each of the 78 children in this study participated in topic continuity sessions consisting of 4 l0-minute play periods with a same-sex peer : 2 in familiar and 2 in unfamiliar situations. Sessions were videotaped and transcribed. Transcripts were analysed by a modified version of the Kertoy and Vetter (1995) topic coding system; categories included topic initiation, topic continuation(collaboration and incorporation), and off-topic. Results showed that children from middle-income families stayed on topic and collaborated more than children from low-income families. Children displayed more topic continuation, collaboration and incorporation in familiar than in unfamiliar situations.

• Korean Journal of Child Studies
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• v.19 no.1
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• pp.169-182
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• 1998

This study investigated the verbal responses of kindergarten children to fantasy and realistic picture books. The subjects were 60 five-year-old children divided into small groups of 4 children each. Each group was read four picture books of which two were fantasy and two were realistic. Transcripts of the children's responses were analyzed for number, content, and type of verbal response. Mean scores and standard deviations were measured and the data were analyzed by repeated one-way ANOVA. Results showed that the children showed (1) more verbal responses to the fantasy than to the realistic picture books. (2) They gave more verbal responses to fantasy picture books than to realistic books in terms of character, scene, event, and vocabulary. They also gave more verbal responses to fantasy picture books than to realistic books in terms of informative, heuristic, and imaginative types.

• English Language & Literature Teaching
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• v.10 no.1
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• pp.77-106
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• 2004

This study aims to investigate how the four college students interact with one another to discuss and construct meaning in a small reading group. Additionally, this study attempts to examine how the participants played their roles in the group. Data sources consisted of transcripts of the students' interactions, questionnaires and informal interviews, and the researchers' observation notes. The data revealed that the participants contributed fairly steadily to the interactions by checking with own grammatical knowledge, providing lexical information, understanding the micro level context, and presenting the macro level context. Several pedagogical implications are presented for the practical classroom. Findings of the study suggest effective ways to implement group activities in reading classes and a teacher's role for optimum group learning.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.5
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• pp.1266-1272
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• 2016

Phospholipase C is a key enzyme of signaling pathways hydrolyzed phosphatidylinositol 4,5-bisphosphate to generate 2 second messengers. Among the PLC, $PLC-{\beta}$ subfamily consisted of 4 isoforms, $PLC-{\beta}$ 1~4. Here, we studied the tissue specific expression of $PLC-{\beta}3$ in olive flounder (Paralichthys olivaceus) following external stimulation like lipopolysaccharide (LPS), concanavalin A (ConA) and environmental stress compared with the inflammatory cytokines IL-1b. $PoPLC-{\beta}3$ gene transcripts has the effect in stimulated tissue compared to control. These results provide what we sure to be a important role for $PLC-{\beta}3$ activity in tissue and verify $PLC-{\beta}3$ as potential immune enzyme for signal transduction.

• Animal cells and systems
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• v.9 no.3
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• pp.127-133
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• 2005

We investigated expression of cucumber senescence-associated genes (SAGs) from developing cotyledons and flower petals. Several cucumber SAGs have been reported in earlier reports. This is an extension of the previous findings. Semi-quantitative RT-PCR revealed that most of the cucumber SAG transcripts were consistently produced until the organ senescence. These results imply that many cucumber senescence-related genes are still active during the final development stage, playing some executive biological roles, possibly in remobilization of nutrients to the other parts of tissues or organs. These results were used to search for possible functions of senescence-related genes during organ development.