• Journal of Korean Society of Forest Science
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• v.83 no.3
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• pp.286-298
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• 1994

The object of this study was to examine the user's impacts on the environmental deteriorations of trail at Ticket Office - Paekryunsa (Temple) Hyangch$\hat{o}$kpong - Dongyupryung - Chilyun Fall area in T$\hat{o}$kyusan National Park. Four trails were sampled in the study area according to the amount of users. Then the user's impacts on trail were measured at each trail. The Ticket Office-Paekryunsa trail was the most used district and followed at Paekryunsa-Hyangch$\hat{o}$kpong trail, Hyangch$\hat{o}$kpong-Dongyupryung trail in descending order. Dongyupryung-Chilyun Fall trail is not used by people because of rest rotation system. The entire width of trail was greater at the more heavily used trail. Maximum depth, cross-sectional area loss, and surface texture and roughness of trail were the highest at Paekryunsa-Hyangch$\hat{o}$kpong trail. Soil hardness, soil acidity, soil moisture content, organic matter content, and exchange canon were influenced by trampling. Soil hardness, soil acidity and exchange canon increased in tramples soil, but content of soil moisture and organic matter decreased therein. Environmental deteriorations of trail were significantly influenced by the amount of users and the slope of trail. Bared lands about $2.000m^2$ were appeared by trampling and camping around Hyangch$\hat{o}$kpong. Effects of carrying of rest rotation system for National Park were partly recognized at Dongyupryung-Chilyun Fall trail.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.138-143
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• 2023

Liver fibrosis is caused by metabolic problems such as cholestasis, genetic problems, or viral infections. Inhibiting hepatic stellate cell (HSC) activation or inducing selective apoptosis of activated HSCs is used as a treatment strategy for liver fibrosis. It has been reported that when HSCs are activated, their apoptosis sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is enhanced because the expression of death receptor 5 is elevated. Finding a natural compound that can enhance the apoptotic effect of TRAIL on HSCs is a necessary strategy for liver fibrosis treatment. It was confirmed here that mangosteen-derived gartanin increased the effect of TRAIL-induced apoptosis by increasing the expression of DR5 in a p38-dependent manner in the hepatic stellate cell line LX-2. Combined treatment with gartanin and TRAIL accelerated DNA cleavage through caspase-3 activation and enhanced antifibrotic effects in LX-2 cells.

• Journal of the Korean Society of Costume
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• v.65 no.3
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• pp.151-166
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• 2015

This research aims to study the needs of the trail runners as trail running has become a popular outdoor activity, and give suggestions for high functional outdoor menswear design that can satisfy the needs of the highly demanding taste of runners. The design development is as follows: 1) The design had to be made of lightweight material and be easily packable in all situations, and 2) we also considered the ergonomic and compact fit for activity, 3) the functional location of high functional fabric, 4) the reflective use and layering system giving a wide range of outdoor workout time and 5) the trend in 2016 S/S active sports and outdoor wear. Based on the above elements, the men's outdoor wear design has been developed for trail running in spring and summer. The design development includes a total of 7 items. The design focused on lightweight, availability of packaging, "comfortability" and freshness in activity, functional suitability of location of highly functional materials and the layering system to protect body temperature under the changing environment. In particular, the layering system was implemented to provide ventilation, and it was done in body parts that released the most body heat. Therefore, mesh materials were actively used on the side panel, sleeves and center of the back piece for necessary sweat emission without any problems and ventilation for trail running.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1471-1476
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• 2012

Background and Aim: Currently available systemic therapies for malignant melanoma produce low response rates in patients, and more effective treatment modalities are clearly needed. The tumor necrosis factor (TNF)-related apoptosis-inducing ligand has a significant impact on therapy for patients with X-linked inhibitor of apoptosis protein-downregulation malignant melanoma. The primary objective of this study was to assess its therapeutic potential. Materials and Methods: We employed a conditionally replicating oncolytic adenoviral vector, named CRAd5.TRAIL/siXIAP, with the characteristics of over-expression of the therapeutic gene TRAIL and downregulation of XIAP in one vector. B16F10-luc cells were employed to detect anti-tumor activity of CRAd5.TRAIL/siXIAP in vitro and in vivo. Results: CRAd5.TRAIL/siXIAP enhanced caspase-8 activation and caspase-3 maturation in B16F10 cells in vitro. Furthermore, it more effectively infected and killed melanoma cells in vitro and in vivo than other adenoviruses. Conclusion: Taken together, the combination of upregulation of TRAIL and downregulation of siXIAP with one oncolytic adenoviral vector holds promise for development of an effective therapy for melanomas and other common cancers.

• Journal of Microbiology and Biotechnology
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• v.27 no.12
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• pp.2156-2164
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• 2017

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered as an antitumor agent owing to its ability to induce apoptosis of cancer cells without imparting toxicity toward most normal cells. TRAIL is produced in poor yield because of its insoluble expression in the cytoplasm of E. coli. In this study, we achieved soluble expression of TRAIL by fusing maltose-binding protein (MBP), b'a' domain of protein disulfide isomerase (PDIb'a'), or protein disulfide isomerase at the N-terminus of TRAIL. The TRAIL was purified using subsequent immobilized metal affinity chromatography and amylose-binding chromatography, with the tag removal using tobacco etch virus protease. Approximately 4.5 mg of pure TRAIL was produced from 125 ml flask culture with a purification yield of 71.6%. The endotoxin level of the final product was $0.4EU/{\mu}g$, as measured by the Limulus amebocyte lysate endotoxin assay. The purified TRAIL was validated and shown to cause apoptosis of HeLa cells with an $EC_{50}$ and Hill coefficient of $0.6{{\pm}}0.03nM$ and $2.41{\pm}0.15$, respectively. The high level of apoptosis in HeLa cells following administration of purified TRAIL indicates the significance and novelty of this method for producing high-grade and high-yield TRAIL.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.230-247
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• 2023

This study was conducted on migrants in 5 cities and counties near the Jirisan Dulle-gil Trail, designated as a National Forest Trail, to find out how the National Forest Trail affects the quality of life after migrants from urban to mountain villages. The group that used the Jirisan Dulle-gil Trail before and/or after the migration showed higher levels of impact on the migration decision, life satisfaction, and behavioral intention than the group that did not use the trail. The group that was affected by the Jirisan Dulle-gil Trail in deciding on the migration also showed higher usage satisfaction with the Jirisan Dulle-gil Trail, life satisfaction, satisfaction with the migration, and behavioral intention than the unaffected group. There were also significant differences in the quality of life according to the migration area, location satisfaction among the migration satisfaction levels, and behavioral intention. In conclusion, it was confirmed that the Jirisan Dulle-gil Trail plays an important role in the decision to migrate to mountain villages and the quality of life after the migration. The results of this study are expected to be used as basic data to present policies related to National Forest Trails that can contribute to the development of mountain villages and countermeasures against population extinction in mountain villages.

• Korean Journal of Agricultural Science
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• v.31 no.2
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• pp.87-96
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• 2004

This study concentrates on the trail of the CNU(Chungnam National University) forest. The amount of use and the pattern of damage are considered, as well as the extent of environmental damage. Finally, some managerial strategies are proposed in connection with sustainable maintenance. The trail of the university forest is composed of three sections; one is the neighborhood park and the Institute of Information Technology/Communications, the second is the Institute of Information Technology/Communications and the Dormitory, and the third section is the Dormitory and the Foreign prof's Apt. The total length of the trail is 2.6km. The daily amount of use is 1,269 visitors per day. The amount of using increased three times the amount of that in 1998. This study shows the pattern of damage of the trail in the order of root-exposure, widening, rock-exposure, deepening and the divergence in the frequency of occurrence of all these factors. Environmental damage is also graded thus: grade 3 is 27.4m, grade 4 is 0.8m, grade 5 is 1.5m, and grade 6 is 0.2m. Accordingly, some managerial strategies were proposed in connection with the sustainable trail maintenance.

• Journal of Korean Society of Rural Planning
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• v.22 no.3
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• pp.21-31
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• 2016

This is a comparative study focused on the scenic landscape preferences derived from visitors's perceptions at the Dulle-gil and the uphill trail in Bukhansan National Park. Unlike previous landscape assessment methods, this study contains more direct and on-site analysis with methods of understanding the visitors' perceptions by using 'visitor employed photography(VEP)' which requires each visitor to take preferable scenic landscape with one's own smart phone camera. The scenic landscape types at two different trails were categorized and then the types favoring most of participants were verified. There are total number of 16 visual landscape types preferred by participants at both the Dulle-gil and the uphill trail in Bukhansan National Park. As in terms of consensus photography(CP) on the Dulle-gil was found to have more diverse elements including both natural and artificial elements compare to the uphill trail. Furthermore, the perceptually excited node(PEN) at the Dulle-gill were found to be more scattered evenly whereas PEN at the uphill trail were concentrated on the entrance and the top of the mountain. It is necessary to consider that the scenic landscape management at the Dulle-gil and the uphill trail require different approaches. Facilities were set based on careful consideration of distinctive geographical features and vegetation in the Dulle-gil. And the management need to be focus on the 'forested area' maintaining natural state in the uphill trail. Futhermore, the result of the CP and PEN in the trails would be helpful to proceed the next step of landscape perception study depending on characteristics of groups.

• Journal of Life Science
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• v.22 no.5
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• pp.697-701
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• 2012

Activated protein C (APC) has an anticoagulant effect and a non-hemostatic effect such as regulation of cell metastasis and modulation of inflammation. In this study, we investigated whether APC could modulate apoptosis in cancer cells. Tumor necrosis factor (TNF)-${\alpha}$, cyclohexamide, and FAS markedly induced apoptosis in human renal carcinoma Caki cells. When Caki cells were pretreated with APC, the percentage of death receptor-induced apoptosis did not change. Furthermore, we checked the effect of APC on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human glioma T98G and human breast carcinoma MDA231 cells. APC also had no effect on TRAIL-induced apoptosis in both cell lines. However, pretreatment with APC inhibited combination treatment (kahweol plus TRAIL and kahweol plus melatonin)-induced apoptosis and PARP cleavage in Caki cells. Taken together, our results suggest that APC can modulate anti-cancer therapeutic efficiency.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3477-3481
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• 2012

Purpose: To investigate the killing effect on OS cells of a combination of oxaliplatin and TRAIL and related molecular mechanisms. Methods: TRAIL and oxaliplatin were applied to OS732 cells singly or jointly and survival inhibition rates were measured by MTT assay, changes of cellular shape being assessed with inverted phase contrast and fluorescence microscopy. Apoptotic rates were analyzed by flow cytometry (FCM) and immunocytochemistry was used to examine Mcl1 expression of OS732 cells. Results: The survival inhibition rate of combined application of $100{\mu}g/ml$ TRAIL and $1{\mu}g/ml$ oxaliplatin on OS-732 cells was significantly higher than that of either agent singly (p<0.01). Changes of cellular shape and apoptotic rates also indicated apoptosis-inducing effects of combined application to be much stronger than those of individual application. Oxaliplatin had the effect of down-regulating Mcl1 expression and sensitizing OS cells to TRAIL-induced apoptosis. Conclusion: A combination of TRAIL and oxaliplatin exerts strong killing effects on OS-732 cells which might be related to down-regulation of Mcl1 expression.