• Korean Journal of Environment and Ecology
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• v.26 no.6
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• pp.923-933
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• 2012

The purpose of this study targeting Choansan(Mt.) Neighborhood Park in Seoul of South Korea, whose level of visitation and use pressure has been recently increasingly high, was to understand distribution and damage status of forest trail and accordingly, set up direction of how to improve forest trail in urban area. With regards to current damage on forest trail in Choansan(Mt.) Neighborhood Park, the damaged trail with road width of over 2m and with erosion depth of over 30cm amounted to 20.3% and 36.3% respectively. And the trail section with bare land erosion, root exposure or rock exposure and the section whose impact rating class exceeded IV occupied 47.0% and 70.6%, indicating the forest trail was severely damaged. The severely injured trail route mainly included the main forest trail formed along the main ridge, the byroad connected to the main forest trail and the steep forest trail in low-lying area. Based on the study results, five types of restoration of forest trail in Choansan(Mt.) Neighborhood Park were offered, including prevention of forest trail extension, stabilization of forest trail base, maintenance of forest trail surface, vegetation restoration after closing forest trail and maintenance. Ecological restoration was additionally offered. The prevention of forest trail extension was planned to prevent expanded width of forest trail where bare land was exposed. The stabilization of forest trail base was planned to prevent erosion in the forest trail and exposure of roots. The maintenance of forest trail surface was planned in a way to protect the severely damaged forest trail surface by using wood deck and wood stairs and surfacing the road.

• Journal of Life Science
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• v.24 no.9
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• pp.927-934
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• 2014

Sanguinarine, a benzophenanthridine alkaloid originally derived from the root of Sanguinaria canadensis, has been shown to possess antimicrobial, antioxidant, and anti-cancer properties. Although tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is known to induce apoptosis in cancer cells, but not most normal cells and has shown efficacy in a phase 2 clinical trial, development of resistance to TRAIL by tumor cells is a major roadblock. Our previous study indicated that treatment with TRAIL in combination with subtoxic concentrations of sanguinarine sensitized TRAIL-mediated apoptosis in TRAIL-resistant human gastric carcinoma AGS cells; however, the detailed mechanisms are not fully understood. In this study, we show that sanguinarine sensitizes AGS cells to TRAIL-mediated apoptosis as detected by MTT assay, agarose gel electrophoresis, chromatin condensation and flow cytometry analysis. Combined treatment with sanguinarine and TRAIL effectively induced expression of death receptor (DR) 5 but did not affect expression of DR4 and mitogen activated protein kinases signaling molecules. Moreover, the combined treatment with sanguinarine and TRAIL increased the generation of reactive oxygen species (ROS); however, N-acetylcysteine, ROS scavenger, significantly recovered growth inhibition induced by the combined treatment. Taken together, our results indicate that sanguinarine can potentiate TRAIL-mediated apoptosis through upregulation of DR5 expression and ROS generation.

• Journal of Korean Society of Rural Planning
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• v.17 no.3
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• pp.55-65
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• 2011

Long distance trail or trail system planning is the first important step in transforming your vision into reality. Planning presents a vision for a trail or trail system and brings a comprehensive, long-range perspective. The master plan provides solid, credible recommendations for developing a trail or trail system that is safe, convenient, well used, supported by local residents, practicality to implement, and customized to meet the needs of the community, you will need to follow a logical planning. The key elements of master planning includes site assessment, vision, goals and objectives, routing and design, implementation strategies. Trails or trail systems should provide linkages to popular destinations, safely accommodate a variety of users, and be sensitive to any negative impacts on the natural environment and wildlife. Trails planners also need to think about how the trail, or trail system will function in the future as areas are developed or trail population increases. All of these factors during the planning process will ensure the existence of high-quality facilities for years to come. Project for Nakdong-jungmaek trail planning combine long distance trail with circuit way. That project is a planning brought out the best in each of Tokai natural way and Cotswold way. That is planning which is combined a wooded trail in Tokai natural way with access and facilities improving economy in Cotswold way. Also That planning embraces a core cultural center which is concerned forest or wood to come more people.

• Korean Journal of Veterinary Research
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• v.44 no.2
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• pp.195-200
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• 2004

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family and potent inducer of apoptosis. TRAIL has been shown to effectively limit tumor growth in vivo without detectable cytotoxic side effects. Interferon (IFN)-${\gamma}$ often modulates the anti-cancer activities of TNF family members including TRAIL. We previously reported that IFN-${\gamma}$ enhanced TRAIL-induced Apoptosis in HeLa cells without the unknown mechanism. In this study, we investigated whether IRF-1 involves in IFN-${\gamma}$-enhanced TRAIL-induced apoptosis. We exposed HeLa cells to IFN-${\gamma}$ for 12 hours and then treated with recombinant TRAIL protein. No apoptosis was induced in cells pretreated with IFN-${\gamma}$, and TRAIL only induced 30% apoptosis after 3 hours treatment. In HeLa cells pretreated with IFN-${\gamma}$, TRAIL induced cell death to more than 75% at 3 hours, showed that IFN-${\gamma}$-pretreatment enhanced HeLa cell death to TRAIL-induced apoptosis. To investigate the functional role of IRF-1 in IFN-${\gamma}$-enhanced TRAIL-induced apoptosis, IRF-1 was overexpressed by using an adenoviral vector AdIRF-1. IRF-1 overexpression increased apoptotic cell death and significantly enhanced apoptotic cell death induced by TRAIL when infected cells were treated with TRAIL. Our findings show that IFN-${\gamma}$ enhances TRAIL-induced apoptosis by IRF-1 in HeLa cells.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1363-1369
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• 2013

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis by targeting cancer cells. However, some cancer cells are resistant to TRAIL-induced cytotoxicity. One method of overcoming TRAIL resistance is combination treatment with reagents to sensitize cells to TRAIL. Luteolin, a flavonoid, has been shown to have anti-cancer effects by inducing apoptosis and cell cycle arrest in various cancer cell lines in vitro. In this study, we investigated the effects of combination treatment with non-toxic concentration of TRAIL and luteolin in T24 human bladder cancer cells. Combined treatment with luteolin and TRAIL significantly inhibits cell proliferation via activation of caspases by inducing Bid truncation, up-regulation of Bax and down-regulation of X-linked inhibitor of apoptosis protein (XIAP). However, the apoptotic effects of combination treatment with luteolin and TRAIL were significantly inhibited by specific caspases inhibitors. Taken together, these results indicate that combination treatment with TRAIL and luteolin can induce apoptosis in TRAIL-resistant cancer cells through down-regulation of XIAP and modulation of tBid and Bax expression.

• Journal of Life Science
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• v.21 no.11
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• pp.1549-1557
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• 2011

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can selectively induce apoptosis in many types of transformed cells; however, some human hepatocellular carcinoma cells are particularly resistant to the effects of TRAIL. Although genistein, a natural isoflavonoid phytoestrogen, has been shown to have pro-apoptotic activity against human cancer cell lines, little is known about the mechanism of genistein in terms of TRAIL-induced apoptosis. In the present study, it was investigated whether or not combined treatment with genistein and TRAIL synergistically induced apoptosis in Hep3B hepatocarcinoma cells. Results indicate that treatment with TRAIL in combination with nontoxic concentrations of genistein sensitized TRAIL-resistant Hep3B cells to TRAIL-induced apoptosis, which was associated with mitochondrial dysfunction. Further, the inhibition of p38 mitogen-activated protein kinase (MAPK) activation markedly decreased genistein and TRAIL-induced cell viability and apoptosis by enhanced truncation of Bid, increase of pro-apoptotic Bax, decrease of anti-apoptotic Bcl-2, and release of cytochrome c from mitochondria to cytoplasm. Activation of caspases and degradation of poly (ADP-ribose) polymerase induced by the combined treatment was also markedly increased by the inhibition of p38 MAPK, through the mitochondrial amplification step. In conclusion, our data suggest that genistein sensitizes TRAIL-induced-apoptosis via p38 MAPK-dependent pathway.

• Journal of the Korean School Mathematics Society
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• v.13 no.2
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• pp.289-301
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• 2010

This study discovered that instructional objectives of graphs which are dealt with in Math I of the revised curriculum are not matched with those of Discrete Mathematics in the 7th Curriculum. Based on the findings, this study analysed didactic transposition method of trail in graph and matrix of Math I and students' understanding about trail. Then this study discovered that though the concept definition of trail in Math I of the revised curriculum, some textbooks and students tend to consider it as the path. The concept definition of trail is significant in systems that deal with Euler Circuits(Euler Closed trail) and Hamilton Cycle. Then it is not easy to find the value of trail in Math I of the revised curriculum.

• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.523-534
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• 1998

Six trails of Naesorak(west Sorak) district of Soraksan National Park were selected to investigate the use impacts on environmental edterioration of trail according to the different amount of use. The entire width, and slope of trail as the trail condition surveyed at the total of 132 points were significantly varied with the amount of use. Major deterioration types of trail were rock-exposure, root-exposure, deepening and divergence in order of frequency. Deteriorated points were significantly different in trail conditions from non-deteriorated points, and these latter generally appeared at the lowed altituede than the former on each trail. Naesorak district still seemed to have poorer use-impacts than Oesarak(east sorak) district. The dominant species in upper layer of trail edge vegetation differed from trail to trail, but in shrub layer Lespedeza maximowiczii, lindera obutsiloba for valley trail and Rhododendron schlipenbavhii for slope trail. The species diversity and coverage of shrub layer in trail edge were the highest on the Ose'am trail and each trail was dissimilar in species composition of shrub layer of edge vegetation.

• Tuberculosis and Respiratory Diseases
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• v.65 no.6
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• pp.476-486
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• 2008

Background: TRAIL (TNF-related apoptosis inducing ligand) is a newly identified member of the TNF gene family which appears to have tumor-selective cytotoxicity due to the distinct decoy receptor system. TRAIL has direct access to caspase machinery and induces apoptosis regardless of p53 phenotype. Therefore, TRAIL has a therapeutic potential in lung cancer which frequently harbors p53 mutation in more than 50% of cases. However, it was shown that TRAIL also could activates $NF-{\kappa}B$ in some cell lines which might inhibit TRAIL-induced apoptosis. This study was designed to investigate whether TRAIL can activate $NF-{\kappa}B$ in lung cancer cell lines relatively resistant to TRAIL-induced apoptosis and inhibition of $NF-{\kappa}B$ activation using proteasome inhibitor MG132 which blocks $I{\kappa}B{\alpha}$ degradation can sensitize lung cancer cells to TRAIL-induced apoptosis. Methods: A549 (wt p53) and NCI-H1299 (null p53) lung cancer cells were used and cell viability test was done by MTT assay. Apoptosis was confirmed with Annexin V assay followed by FACS analysis. To study $NF-{\kappa}B$-dependent transcriptional activation, a luciferase reporter gene assay was used after making A549 and NCI-H1299 cells stably transfected with IgG ${\kappa}-NF-{\kappa}B$ luciferase construct. To investigate DNA binding of $NF-{\kappa}B$ activated by TRAIL, electromobility shift assay was used and supershift assay was done using anti-p65 antibody. Western blot was done for the study of $I{\kappa}B{\alpha}$ degradation. Results: A549 and NCI-H1299 cells were relatively resistant to TRAIL-induced apoptosis showing only 20~30% cell death even at the concentration 100 ng/ml, but MG132 ($3{\mu}M$) pre-treatment 1 hour prior to TRAIL addition greatly increased cell death more than 80%. Luciferase assay showed TRAIL-induced $NF-{\kappa}B$ transcriptional activity in both cell lines. Electromobility shift assay demonstrated DNA binding complex of $NF-{\kappa}B$ activated by TRAIL and supershift with p65 antibody. $I{\kappa}B{\alpha}$ degradation was proven by western blot. MG132 completely blocked both TRAIL-induced $NF-{\kappa}B$ dependent luciferase activity and DNA binding of $NF-{\kappa}B$. Conclusion: This results suggest that inhibition of $NF-{\kappa}B$ can be a potentially useful strategy to enhance TRAIL-induced tumor cell killing in lung cancer.

• BMB Reports
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• v.49 no.5
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• pp.282-287
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• 2016

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a homo-trimeric cytotoxic ligand. Several studies have demonstrated that incorporation of artificial trimerization motifs into the TRAIL protein leads to the enhancement of biological activity. Here, we show that linkage of the isoleucine zipper hexamerization motif to the N-terminus of TRAIL, referred as ILz(6):TRAIL, leads to multimerization of its trimeric form, which has higher cytotoxic activity compared to its native state. Size exclusion chromatography of ILz(6):TRAIL revealed possible existence of various forms such as trimeric, hexameric, and multimeric (possibly containing one-, two-, and multi-units of trimeric TRAIL, respectively). Increased number of multimerized ILz(6):TRAIL units corresponded with enhanced cytotoxic activity. Further, a high degree of ILz(6):TRAIL multimerization triggered rapid signaling events such as activation of caspases, tBid generation, and chromatin condensation. Taken together, these results indicate that multimerization of TRAIL significantly enhances its cytotoxic activity.