• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3477-3481
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• 2012

Purpose: To investigate the killing effect on OS cells of a combination of oxaliplatin and TRAIL and related molecular mechanisms. Methods: TRAIL and oxaliplatin were applied to OS732 cells singly or jointly and survival inhibition rates were measured by MTT assay, changes of cellular shape being assessed with inverted phase contrast and fluorescence microscopy. Apoptotic rates were analyzed by flow cytometry (FCM) and immunocytochemistry was used to examine Mcl1 expression of OS732 cells. Results: The survival inhibition rate of combined application of $100{\mu}g/ml$ TRAIL and $1{\mu}g/ml$ oxaliplatin on OS-732 cells was significantly higher than that of either agent singly (p<0.01). Changes of cellular shape and apoptotic rates also indicated apoptosis-inducing effects of combined application to be much stronger than those of individual application. Oxaliplatin had the effect of down-regulating Mcl1 expression and sensitizing OS cells to TRAIL-induced apoptosis. Conclusion: A combination of TRAIL and oxaliplatin exerts strong killing effects on OS-732 cells which might be related to down-regulation of Mcl1 expression.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.15
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• pp.5977-5982
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• 2014

Research over the years has progressively shown substantial broadening of the tumor necrosis factor alpha-related apoptosis-inducing ligand (TRAIL)-mediated signaling landscape. Increasingly it is being realized that pancreatic cancer is a multifaceted and genomically complex disease. Suppression of tumor suppressors, overexpression of oncogenes, epigenetic silencing, and loss of apoptosis are some of the extensively studied underlying mechanisms. Rapidly accumulating in vitro and in vivo evidence has started to shed light on the resistance mechanisms in pancreatic cancer cells. More interestingly a recent research has opened new horizons of miRNA regulation by DR5 in pancreatic cancer cells. It has been shown that DR5 interacts with the core microprocessor components Drosha and DGCR8, thus impairing processing of primary let-7. Xenografting DR5 silenced pancreatic cancer cells in SCID-mice indicated that there was notable suppression of tumor growth. There is a paradigm shift in our current understanding of TRAIL mediated signaling in pancreatic cancer cells that is now adding new layers of concepts into the existing scientific evidence. In this review we have attempted to provide an overview of recent advances in TRAIL mediated signaling in pancreatic cancer as evidenced by findings of in vitro and in vivo analyses. Furthermore, we discuss nanotechnological advances with emphasis on PEG-TRAIL and four-arm PEG cross-linked hyaluronic acid (HA) hydrogels to improve availability of TRAIL at target sites.

• Journal of Korean Society of Rural Planning
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• v.21 no.1
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• pp.143-154
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• 2015

This study has set up a plan to establish a trail as a means of maximizing a community by using the residents' living materials and unused facilities that a village holds now. A local archive is a place where the records and living materials collected from a village are preserved, displayed and shared with others, serving as a complex space with multiple functions. To apply the idea of 'utilizing a local archive', the empirical study was conducted by making an analysis of trail resources of Baekryeong Island and local archive application resources. Local archive resources were selected through evaluation of connection of trail routes by investigating and discovering the unused facilities of Baekryeong Island village and the depreciation level and scale of them. And actual local archive living references were investigated and collected through in-depth interviews of villagers. Also, through planning of trails by region, limitations of investigation activities were improved and various tangible and intangible resources owned by Baekryeong Island were utilized to enable effective and distinctive investigation activities and experiences. Unused facilities were surveyed and chosen to take advantage of the local archive space, along with in-depth interviews with the residents living in Baekryeong Island. In addition, the current conditions for the trail routes were checked out in order to 'point out the final routes'.

• Korean Journal of Environment and Ecology
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• v.15 no.3
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• pp.257-266
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• 2001

Trail might be the primary recreational space to appear the use impact by visitors on ecological space in National park. On the basis of this concept, the carrying capacity was estimated on the trail. Impact rating class and pattern of passersby were surveyed on the 22 valid trails in Bukhansan National Park. Using two variables, the width of trail and amount of passersby, the correlation coefficient was analysed and the regression model was derived by raising x to a higher power. And carrying capacity estimation equation was devised by considering the pay fee visitors and average passersby in a trail section. With carrying capacity estimation equation to apply Bukansan National Park, it is desired that maximum carrying capacity is about 3 million persons a year under conditions of trail width 2.2m when National Park Authority wants to keep the existing management level. If they strengthen the management goal far resource conservation like that they want to keep the trail width 2.0m, the number of visitors might be decreased to about 2 million persons a year.

• Journal of the Korean association of regional geographers
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• v.19 no.2
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• pp.234-245
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• 2013

In order to examine the amount and rate of soil erosion on Ridge Traversing Trail in Jirisan National Park, a cross-sectional area of hiking trail were monitored at 16 sites in Nogodan - Samdobong section from November 2011 to April 2012. Although all sites demonstrates an enlarged cross-section of trail, the amount of soil erosion varies from site to site: 54.9 to $908.8cm^2$. It suggests that the erosional rate ranges from $0.1cm^2/day$ to $1.72cm^2/day$. The erosional amount is also varied with a trail type: $109.3cm^2$ for a shallow gully-like trail to $573.2cm^2$ for a unilateral trail. However, the cross-sectional change is larger on a sidewall than a tread irrespective of a trail type. The erosional amounts of November to April are smaller than that of May to October. In particular, the erosional amount of November 2011 to April 2012 is smaller than the depositional amount, implying a reduced cross-section of trail. Pipkrake action puts loose soil particles on a sidewall on March and April, and then rainwash due to a heavy rainfall takes them away after May. It seems to be the most predominant erosional process in Ridge Traversing Trail. A sidewall facing north shows a larger amount of erosion than a sidewall facing south. It also implies a difference in the development of a pipkrake according to an aspect. The small amount of erosion and cross-sectional decrease, which is usually observed on April, results from the combined effect of frost heaving, pipkrake action, a small rainfall and a temporary suspension of trampling. It is necessary to establish the monitoring system of trail erosion in terms of the management of hiking trail in a mountain national park.

• Journal of Life Science
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• v.18 no.1
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• pp.120-128
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• 2008

The death ligand tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/ Apo1L is a cytokine that activates apoptosis through cell surface death receptors. TRAIL has sparked growing interest in oncology due to its reported ability to selectively trigger cancer cell death. Euphorbiae humifusae Wind has been used in traditional Oriental medicine as a folk remedy used for the treatment of cancer. However, the mechanism responsible for the anticancer effects of E. humifusae not clearly understood. Here, we show that treatment with subtoxic doses of water extract of E. humifusae (WEEH) in combination with TRAIL induces apoptosis in TRAIL-resistant human gastric carcinoma AGS cells. Combined treatment with WEEH and TRAIL induced chromatin condensation and sub-G1 phase DNA content. These indicators of apoptosis were correlated with the induction of caspase activity that resulted in the cleavage of poly (ADP-ribose) polymerase. Combined treatment also triggered the loss of mitochondrial membrane potential. Furthermore, co-treatment with WEEH and TRAIL down-regulated the protein levels of the anti-apoptotic proteins such as Bcl-2, Bcl-xL, XIAP and cIAP-1. Although more study will be needed to examine the detailed mechanisms, this combined treatment may offer an attractive strategy for safely treating gastric adenocarcinomas and the results provide important new insights into the possible molecular mechanisms of the anticancer activity of E. humifusae.

• Tuberculosis and Respiratory Diseases
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• v.63 no.1
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• pp.42-51
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• 2007

Background: TRAIL is a cytokine that selectively induces apoptosis in various cancer cell lines. Gefitinib is new targeted drug applied in lung cancer that selectively inhibits EGFR tyrosine kinase. However, lung cancers have shown an initial or acquired resistance to these drugs. This study examined the effect of IGF-1R and its blockade on enhancing the sensitivity of lung cancer cell lines to TRAIL and gefitinib. Methods: Two lung cancer cell lines were used in this study. NCI H460 is very sensitive to TRAIL and gefitinib. On the other hand, A549 shows moderate resistance to TRAIL and gefitinib. The IGF-1R blockade was performed using adenoviruses expressing the dominant negative IGF-1R and shRNA to IGF-1R and AG1024 (IGF-1R tyrosine kinase inhibitor). Results: The adenovirus expressing dominant negative IGF-1R(950st) induced the increased expression of defective IGF-1R on the lung cancer cell surface, and the adenovirus-shIGF-1R effectively decreased the level of IGF-1R expression on cell surface. The genetic blockade of IGF-1R by the adenovirus-dnIGF-1R and AG1024 increased the sensitivity of A549 cells to TRAIL. The reduction of IGF-1R by transduction with ad-shIGF-1R also increased the sensitivity of the A549 cells to gefitinib. Conclusion: The blockade of IGF-1R through various mechanisms increased the sensitivity of the lung cancer cell line that was resistant to TRAIL and gefitinib. However, further studies using other cell lines showing acquired resistance as well as in vivo animal experiments will be needed.

• Korean Journal of Environment and Ecology
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• v.24 no.3
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• pp.293-301
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• 2010

The purpose of this study is to figure out visitor's attitudes for trail facilities in Gayasan National Park in order to provide the basic information for effective installation and maintenance of trail facilities. The questionnaire survey on two trails were conducted to 227 visitors on the way back home after using the trails. The use characteristics of two trails were a little different from each other, and the conditions of two trails were rated relatively high both in quantity and quality. By the evaluation of 5 types of trail facilities in Haein trail and 4 types in Baegun trail, The responses were noticeably positive about wood deck, log steps with stones and wood steps in Haein trail and about deck-type wood steps and log fences in Baegun trail from the point of three aspects; harmony with nature, resources conservation and user convenience. On the other hand it seems necessary to replace the steel steps of both trails with eco-friendly materials. So, from now on it is necessary for introducing the trail facilities in National Parks to apply the eco-friendly materials extensively and to upgrade the design aspects comprehensively of harmony with nature, resources conservation and user convenience

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.352-359
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• 2015

This study was conducted to evaluate how the special protection area designations of degraded trails effect on the soil and vegetation recovery of degraded trails. The study areas were established on an opened trail and a 16-year closed trail, which was prohibited to enter after the designation as a special protection area for recovery, at Bukhansan National Park. Soil core sampling and measurements of vegetation cover were performed to compare soil and vegetation properties of the trails. Soil bulk density increased and soil water total nitrogen decreased on the opened trail, while no significant differences were found on bulk density, soil water, total nitrogen, acidity, and organic matter on the closed trail. On the opened trail, vegetation cover was seemed to be degraded, because vegetation litter cover ratio was low and barren rock cover ratio was high. On the closed trail, litter rock barren cover ratio of the closed trail was recovered, but only limited recovery was found on vegetation cover by applying environmental damage condition rating class. In conclusion, the closed trail was recovered by designation of special protection area, while difference in recovery progress of soil and vegetation was found. Therefore, designation of special protection area of degraded area should be based on scientific basis of recovery characteristics of the area. In order to improve the effectiveness of special protection area system, further specific standards for special protection area designation and management would be needed, considering ecological and social importance of target areas.

• Journal of the Korean Institute of Landscape Architecture
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• v.17 no.3
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• pp.21-34
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• 1990

Trail and campground deteriorations and their environmental changes of soil and vegetation were studied in Tonghwa -Sa district of Mt. Palgong Natural Park through 1988 into 1989. The results are summarized as follows : 1. The bare width of trail as one of the trail condition factor was significantly greater on the more heavily used trail. Deterioration types of trail which had higher frequency for a total of 45 observations were rock exposure(42%), trail deepening(40%) and root exposure(18%) in the high order. And also sound type on which the trail was not deteriorated at all amounted to 33% of all observations. 2. Ecological changes of soil and vegatation of trailsides were not found at a uniform tendency except that soil hardness was slightly decreased from trail edge to the forest, but they could be grouped by the type of user\`s behavior and site conditions. Use impacts on the natural environment of trailside in Mt. Palgong are still far from the severe harmulness. 3. Worse damages to compground condition were appeared in Bawi-gol than Susu-gol campsite. Types of mechanically damaged trees were tree with exposed root(63% ), scared tree(43% ), mutilated tree(30 % ) and felled tree in highly frequent order. And diameter increments of trees in campsites were oppressed by the campers. 4. Tree damage types and their frequencies could be basic as a means of which grasp the bounds of user's impacts. User's disturbance on campsite extended to the distance of 70∼90m from the core of campsite at Pawi-gol and 20∼30m at Susu-gol respectively. As the tolerant trees to use impact, Lespedeza maximowiczii, Rhus trichocarpa, Acer palmatum, Rubus crataegifolius and Celastrus orbiculatus were considered.