• Journal of Veterinary Science
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• 제25권3호
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• pp.40.1-40.19
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• 2024

Importance: The creation of robust maternal-embryonic interactions and implantation models is important for comprehending the early stages of embryonic development and reproductive disorders. Traditional two-dimensional (2D) cell culture systems often fail to accurately mimic the highly complex in vivo conditions. The employment of three-dimensional (3D) organoids has emerged as a promising strategy to overcome these limitations in recent years. The advancements in the field of organoid technology have opened new avenues for studying the physiology and diseases affecting female reproductive tract. Observations: This review summarizes the current strategies and advancements in the field of 3D organoids to establish maternal-embryonic interaction and implantation models for use in research and personalized medicine in assisted reproductive technology. The concepts of endometrial organoids, menstrual blood flow organoids, placental trophoblast organoids, stem cell-derived blastoids, and in vitro-generated embryo models are discussed in detail. We show the incorportaion of organoid systems and microfluidic technology to enhance tissue performance and precise management of the cellular surroundings. Conclusions and Relevance: This review provides insights into the future direction of modeling maternal-embryonic interaction research and its combination with other powerful technologies to interfere with this dialogue either by promoting or hindering it for improving fertility or methods for contraception, respectively. The merging of organoid systems with microfluidics facilitates the creation of sophisticated and functional organoid models, enhancing insights into organ development, disease mechanisms, and personalized medical investigations.

• Advances in Traditional Medicine
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• 제6권2호
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• pp.69-78
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• 2006

The isoflavonoids comprise a group of phyto-oestrogens that have useful biological activities including oestrogenic, antioxidant and anticancer. As dietary components for humans, they are bioavailable from leguminous vegetables (such as genistein from soybean), and have been well-documented to have numerous health benefits. A wide range of epidemiological studies in humans and limited studies in animals have identified isoflavonoids as potential chemopreventive agents against hormone-dependent cancers. Therefore, an attempt has been made through this review to summarise the information in the mechanisms aspect of isoflavonoid phyto-oestrogens in inhibiting cancer in vitro and in vivo in the models of human cancers.

• 한국임상보건과학회지
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• 제2권1호
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• pp.73-78
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• 2014

Purpose. Citrus aurantium L.(familyRutaceae), alsoknownasbitter orange, have been used as traditional herbal medicine in many Asian countries since ancient times. Hence, the purpose of the study was to briefly discuss the new findings about anti-inflammatory and anti-cancer activities of Citrus aurantium L in-vitro. Methods. The articles for this study were collected from pubmed and scopus electronic resources. Results. Citrus aurantium L contains an abundant Flavonoids, including hesperidin, naringin and nobiletin. These Flavonoids has reported to have various medicinal benefits that include antioxidant, antimicrobial, anti-inflammatory, anticancer, anti-diabetic activities, and also used to treat cardiovascular diseases. Conclusion. Based on the above evidence, we propose that Flavonoids from Korea Citrus aurantium L would be a therapeutic potential for cancer treatment and pharmacological benefit for inhibiting the inflammatory effect.

• 한국임상수의학회지
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• 제24권4호
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• pp.608-612
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• 2007

4년령의 중성화한 수컷 코커 스파니엘이 후지 마비와 진전 때문에 내원 하였다. 본 환축은 X-ray와 MRI를 이용하여 추간판탈출증이 합병된 척수연화증으로 진단되었다. 환축의 치료를 위하여 봉독 약침, 뜸 및 한약제의 투여를 이용하였다. 사용 혈위는 GV02-1, GV04, GV11, GV12, GV13, GV14, BL11, BL18, BL23, BL25, BL28, GB21, HT07, KI03, LI04, LU07, SI03, SI06, ST36, ST38, ST39, ST40, ST41, GB30, GB31, GB34, LIV03, SP06 및 압통점($T1{\sim}T4\;and\;T9{\sim}L1$) 이었다. 그 결과, 1차 치료 시 환축은 기립이 불가능하였으며, 사지를 움직이지 못하였다. 그러나 7차, 14차 및 21차 치료 시 점차적인 임상증상의 개선이 관찰되었다. 비록 한방수의학적 방법으로 임상 증상이 완치된 것은 아니지만, 21차 치료 후 임상증상이 일부 호전된 증례이었다.

• 한국식품영양과학회지
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• 제44권4호
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• pp.497-505
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• 2015

YG-1의 항염증 및 해열 치료 약물로서의 가능성을 검증하기 위하여 대식세포와 동물모델에서 염증성 사이토카인 활성 및 해열 관련 인자들을 연구하여 다음과 같은 결론을 얻었다. YG-1은 염증성 사이토카인 인자인 IL-$1{\beta}$의 발현을 효과적으로 억제하였으며, iNOS 및 COX-2의 발현을 억제시킴으로써 염증인자인 NO 및 $PGE_2$ 생성을 유의적으로 감소시키는 것으로 나타났다. 또한 YG-1은 LPS 유도성 발열 모델에서 염증을 완화시키고 해열의 효과를 보여 주었다. 이상의 실험 결과로부터 본 실험에 사용된 YG-1은 염증에 의해 유발되는 인후염과 같은 질환을 예방 및 치료할 수 있을 것으로 기대된다.

• Journal of Ginseng Research
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• 제48권3호
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• pp.245-252
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• 2024

Ginseng is a traditional herbal medicine used for prevention and treatment of various diseases as a tonic. Recent scientific cohort studies on life prolongation with ginseng consumption support this record, as those who consumed ginseng for more than 5 years had reduced mortality and cognitive decline compared to those who did not. Clinical studies have also shown that acute or long-term intake of ginseng total extract improves acute working memory performance or cognitive function in healthy individuals and those with subjective memory impairment (SMI), mild cognitive impairment (MCI), or early Alzheimer's disease (AD) dementia who are taking AD medication(s). Ginseng contains various components ranging from classical ginsenosides and polysaccharides to more recently described gintonin. However, it is unclear which ginseng component(s) might be the main candidate that contribute to memory or cognitive improvements or prevent cognitive decline in older individuals. This review describes recent clinical contributors to ginseng components in clinical tests and introduces emerging evidence that ginseng components could be novel candidates for cognitive improvement in older individuals, as ginseng components improve SMI cognition and exhibits add-on effects when coadministered with early AD dementia drugs. The mechanism behind the beneficial effects of ginseng components and how it improves cognition are presented. Additionally, this review shows how ginseng components can contribute to SMI, MCI, or early AD dementia when used as a supplementary food and/or medicine, and proposes a novel combination therapy of current AD medicines with ginseng component(s).

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2000년도 추계학술대회 및 정기총회
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• pp.11-11
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• 2000

The development of new investigative technology and the changing roles of other biological and medical disciplines will result in major changes in the way veterinary pathology is practiced in the future. Unless the pathologists strategically prepare themselves to adopt and incorporate these changes into their modest operandi, the veterinary pathologist's traditional dominance in the diagnosis of spontaneous animal diseases and experimentally induced lesions in animals will be at risk. Although the distinguishing feature of pathologist is their artful ability to read and interpret the pathologic changes of cells and tissues, we must strive to enhance and expand our ability beyond and above the simple reading of H＆E stained slides. Also, we must be prepared to meet the ever increasing demand of diagnostic accuracy and timeliness. To do so, we have to employ the new biological and toxicological technologies into our routine. (omitted)

• Journal of Veterinary Science
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• 제22권1호
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• pp.6.1-6.6
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• 2021

Stem cell-based products (SCPs) are an emerging field of veterinary medicine that focuses on the regeneration, repair, or replacement of damaged tissues or organs. However, there are some issues in applying the traditional regulatory guideline for the approval of SCPs as veterinary medicinal products. This article describes the positions of Korea, US, and EU regarding SCPs, and compares the regulatory guidelines of each country for their safety evaluation. Although there are some differences in the regulatory guidelines, similar considerations in identifying the quality of SCPs and their safety has adopted. Overall, these guidelines need to be harmonized among countries.

• 대한의생명과학회지
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• 제13권3호
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• pp.161-168
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• 2007

Salicornia herbacea L. (Chenopodiaceae: S. herbacea) is a salt marsh plant, which has long been prescribed in traditional medicines for the treatment of intestinal ailments, nephropathy, and hepatitis in Oriental countries. In order to elucidate the mechanisms of this herb, we conducted an anti-oxidative activity, the inhibition of nitric oxide (NO) production, and the suppression of the pro-inflammatory cytokine genes, with the solvent-extracts of S. herbacea. We found that both ethyl acetate and n-butanol tractions showed potent anti-oxidative effects in comparison to other fractions using xanthine oxidase assay with $IC_{50}$ values of $66.0{\pm}0.5\;{\mu}g/ml$ and $82.5{\pm}3.8\;{\mu}g/ml$, respectively. In addition, both ethyl acetate and n-butanol fractions showed more electron donating activity (EDA) than other tractions, according to DPPH (2, 2-Diphenyl-lpicrylhydrazyl radical) assay. The EDA of ethyl acetate fraction ($IC_{50}$ values of $117.5{\pm}3.8\;{\mu}g/ml$) is more significant than that of n-butanol fraction ($IC_{50}$ values of $375.0{\pm}12.5\;{\mu}g/ml$). Among potential anti-oxidative tractions, ethyl acetate traction dose-dependently suppressed lipopolysaccharide (LPS, $0.1\;{\mu}g/ml$)-induced nitric oxide (NO) production in RAW264.7 cell, while n-butanol did not. As expected, ethyl acetate fraction suppressed the expression of inducible NO synthase (iNOS) in RAW264.7 cell stimulated by $0.1\;{\mu}g/ml$ of LPS. Moreover, the ethyl acetate traction suppressed the expression of interleukin-1 $(IL)-1{\beta}$ and granulocyte/macrophage colony-stimulating factor (GM-CSF) mRNA in LPS-stimulated RAW264.7 cells. Therefore, these results suggest that S. herbacea may have anti-oxidative and anti-inflammatory activities by modulating radical-induced toxicity and various pro-inflammatory responses.

• Archives of Pharmacal Research
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• 제28권6호
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• pp.680-684
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• 2005

Ginseng, the root of Panax ginseng CA Meyer, is well known as a tonic medicine for restoring and enhancing human health. In traditional medicine, ginseng is utilized for the alleviation of emesis, which includes nausea and vomiting. However, it has not yet been demonstrated whether ginseng exhibits in vivo anti-nausea and anti-vomiting properties. In this study, we examined the anti-emetic effect of Korean red ginseng total extract (KRGE) on cisplatin-induced nausea and vomiting using ferrets. Intraperitoneal administration (i.p.) of cisplatin (7.5 mg/kg) induced both nausea and vomiting with one-hour latency. The episodes of nausea and vomiting reached a peak after 1.5 h and persisted for 3 h. Treatment with KRGE via oral route significantly reduced the cisplatin-induced nausea and vomiting in a dose-dependent manner. The anti-emetic effect was 12.7 $\pm$ 8.6, 31.8 $\pm$ 6.9, and 67.6 $\pm$ 4.0$\%$ with doses of 0.3, 1.0, and 3.0 g/kg of KRGE, respectively. Pretreatment with KRGE via oral route 1 and 2 h before cisplatin administration also significantly attenuated the cisplatin-induced nausea and vomiting. However this did not occur with a pretreatment 4 h before cisplatin administration. These results are supportive of KRGE being utilized as an anti-emetic agent against nausea and vomiting caused by chemotherapy (i.e. cisplatin).