• The Korea Journal of Herbology
• /
• v.23 no.3
• /
• pp.163-173
• /
• 2008

Objectives : Imyosan (IMS), a drug preparation comprised of Phellodendri Cortex (PC) and Atractylodis Rhizoma (AR), is commonly used as a traditional herbal medicine in Korea and China for the treatment of various inflammatory diseases. However, little is known about the effect of IMS and its component herbs on inflammatory mediators in RAW 264.7 cells. Therefore, in this study, methanol extracts of IMS and its component herbs were examined to determine if they inhibited inflammatory effects in RAW 264.7 cells. Methods : Cytotoxic activity of IJHT and its components on RAW 264.7 cells was using 5-(3-carboxymethoxyphenyl)-2H-tetrazolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines were measured by ELISA kit. The levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were detected by western blot. Results : Methanol extract of IMS and its component herbs were significantly reduced iNOS and COX-2 expression as well as NO, PGE2, $IL-1{\beta}$ and IL-6 production in RAW 264.7 cells. Conclusions : The results of this study indicated that the anti-inflammatory effect of Imyosan extract is more potent than that of extracts of its component herbs in macrophages.

• YAKHAK HOEJI
• /
• v.54 no.5
• /
• pp.403-409
• /
• 2010

Gardenia jasminoides is a popular traditional herb used to treat inflammatory diseases including liver disorders. This study was performed to examine protective effect of G. jasminoides on galactosamine (GalN)-induced acute hepatitis. Rats were treated intraperitoneally with GalN (700 mg/kg). G. jasminoides (30, 100 and 300 mg/kg) was administered orally 48, 24, and 2 h before and 6 h after GalN injection. Serum ALT and AST activities were significantly increased after GalN injection, and these increases were attenuated by G. jasminoides. Histological studies showed that G. jasminoides inhibited hepatocellular necrosis with inflammatory cell infiltration. GalN decreased the serum levels of total cholesterol and this decrease was attenuated by G. jasminoides. Hepatic glutathione content was decreased and lipid peroxidation was increased after GalN treatment and these changes were attenuated by G. jasminoides. Furthermore, the level of tumor necrosis factor-${\alpha}$ mRNA expression was significantly increased after GalN injection, and this increase was attenuated by G. jasminoides. The level of interleukin-10 mRNA expression was significantly increased after GalN injection, and this increase was augmented by G. jasminoides. Our results suggest that G. jasminoides ameliorates GalN-induced acute hepatitis and this protection is likely due to antioxidative activity and regulation of inflammatory mediators.

• Journal of Fashion Business
• /
• v.22 no.1
• /
• pp.114-123
• /
• 2018

The purpose of this study is to investigate the visual elements for effectively conveying the make-up method of the Korean history. We will do basic research on language visualization through visual poetry by using a make-up method as a visual expression element. Our research methods consisted of a related literature research, an internet search, and case study methods. The make-up methods of Korean classical make-up through visual poetry consisted of a through literature review. First, make-up methods, cosmetics, jewelry, and hair styles are shown in the classic make-up style of each age. Second, classical make-up visual poetry shows the identity, gender, and character depictions. Third, there were many visual metaphors about the hair types, figures, and hair among the classical make-up methods of each age. I think that this topic is worth researching as an appropriate medium to increase attractiveness. This research is with visual expression elements of the Korea make-up method. This study analyzed the Korean traditional make-up method that expresses and reinterprets visual metaphors. The results of this study can be applied for basic research.

• Proceedings of the Korean Institute of Interior Design Conference
• /
• 2007.11a
• /
• pp.73-78
• /
• 2007

Recently, Townhouse has emerged as a blue ocean of real estate market in Korea. In designing town houses, which are luxurious suburban houses centered around the capital area, the construction companies are using 'designer marketing' in which they hire interior designers and architects and express various styles of design in the interior space of the town houses. Therefore, this study examined the unit-family cases of Korean townhouses and analyzed the expression characteristics of design in the interior space of the town houses built after 2000 in terms of their spatial, morphological, material, and color aspect. "The extensibility of space" and "variableness of space" seem to have the same meaning, but they turned out to be not related to each other due to the particularity of houses. "The extensibility of space" turned out to show its characteristics via "the use of transparent materials." "Minimal form" utilizes the unified "achromatic and simple colors" to emphasize the simplicity and in some cases, highlights the use of original colors. The interior space of the townhouses expresses the traditional or heterosexual oriental image through "the use of natural materials," and they point out the "oriental form" through the controlled beauty expressed by using oriental components and "achromatic and simple colors."

• Journal of Pharmacopuncture
• /
• v.15 no.1
• /
• pp.18-22
• /
• 2012

Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here, we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.27 no.3_4
• /
• pp.310-321
• /
• 2003

The purpose of this study is clarify the formative characteristic of dance costume design and analyse stage effect based upon the formative factors. Classified design element of dance costumes by line, silhouette, color, material, lighting, and also this based on content analysis method about each formative factor of costumes that showed 10 dance performances which practically be performed and made. And individually interviewed the professional dance composers and dancers who participated in the performances fer objectivity and specialty of this study. The results were as follows; 1. Dance costume contains and harmoniously expresses theme of a dance work, formative characteristic of fashion design, movement and character of the dancers, lighting effect. 2. Korean creative dance costume is effectively expressed with formative factors of traditional costumes and modem sensibility and symbolic expression of image-centered. 3. Costume line is affected by dancer's movement and it's possible to be different effect that the atmosphere or stage effect by the original form of line and the expression method. 4. Costume color is effective to inform that dancer's character and image of a dance work. 5. Costume material is most important to consider that the dancers and the atmosphere and the image of a dance work. 6. Lighting effect can inform various feel of dancer's dance.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.20 no.5
• /
• pp.1327-1333
• /
• 2006

Many traditional herbal remedies exhibit several beneficial effects including anti-inflammation. Panax notoginseng Buck F.H. Chen. is used as a therapeutic agent to stop haemorrhages and a tonic to promote health in Korean and Chinese medicine. The pharrnacokinetic profiles of the main P. notoginseng are still not accurately investigated. The exact mechanism of the anti-inflammatory action of P. notoginseng, however, has not been determined. In the present study, we examined the effect of P. notoginseng on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, and inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene expression in RAW264.7 macrophages. The results indicated that P. notoginseng concentration-dependently inhibited LPS-induced NO production. Furthermore, P. notoginseng inhibited the expression of LPS-induced iNOS and COX-2 proteins without an appreciable cytotoxic effect on RAW264.7 cells. Berberine also inhibited LPS-induced iNOS protein as potently as P. notoginseng. This was consistent with the findings that P. notoginseng and also berberine inhibited prostaglandin E2 synthesis induced by LPS.

• Korean Journal of Plant Resources
• /
• v.32 no.4
• /
• pp.275-281
• /
• 2019

The pathological condition of excessive melanogenesis causing freckles, melasma, senile lentigo, pigmented acne scars, and cancer has a critical impact on the wellness of individuals. The mechanism of melanogenesis is related to the expression of melanogenic enzymes. Here, we evaluated the inhibitory effect of pine cone (Pinus densiflora) extracts on melanogenesis. P. densiflora, the Korean Red Pine, is the predominant tree species in the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. P. densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones are rarely used as natural raw materials, although they contain many bioactive phytochemicals. The pine cone ethyl acetate fraction (PEF) showed no toxicity to B16F10 cells at a concentration of less than $100{\mu}g/mL$. PEF inhibited the expression of microphthalmiaassociated transcription factor (MITF), tyrosinase and tyrosinase-related factors in B16F10 cells treated with 3-Isobutyl1-methylxanthine (IBMX). These results suggest that pine cones can be used as an effective natural melanogenesis inhibitory agent.

• Herbal Formula Science
• /
• v.29 no.4
• /
• pp.277-283
• /
• 2021

Objectives : We investigated the effects of Bojungikgi-tang (BJIGT) on P450 cytochrome enzyme and oxidative stress in the cells. Methods : We enrolled the HepG2 hepatocyte cell line to assess MTT assay, flow cytometer, and immunoblotting analysis. Expression of CYP450 was confirmed by immunoblotting analysis in the Huh7 cell line. Results : We determined that BJIKT markdely changed the expression of the CYP2C19, CYP2D6, and CYP2E1. Moreover, BJIKT inhibited the cell toxicity induced by arachidonic acid + iron treatment, as assessed by FACS analysis. BJIKT induced AMPK activation, which increased the phophorylation of ACC. Conclusions : This study verified the effects of BJIKT, on P450, ROS production, mitochondrial damage and AMPK signaling pathway, which might give us the scientific information about the traditional herbal prescription.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.33 no.1
• /
• pp.31-38
• /
• 2019

Cheongpyesagan-tang (CP) is one of the traditional medicinal prescription to treat Taeumin (太陰人)'s disease. It has been commonly used for the treatment of stroke, arthritis, diabetes and obesity. In this study, we investigated an anti-inflammatory potential of CP water extract. We examined the effects of CP on the lipopolysarccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$). We also examined the levels of protein or mRNA of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and proinflammatory cytokines in RAW 264.7 cells. CP inhibited NO and $PGE_2$ production in a dose dependent manner and decreased the protein and mRNA expression of iNOS and COX-2. Also, CP decreased the mRNA expression of interleukin-6 (IL-6), interleukin-$1{\beta}$ (IL-$1{\beta}$), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$). These results suggest that CP has potential as anti-inflammatory therapeutic medicine.