• 디자인학연구
• /
• 제14권3호
• /
• pp.135-144
• /
• 2001

우리의 전통문화 유산에서 새롭게 디자인 모티브를 발굴하고 시각적으로 상징화하는 연구는 과거의 것과 현대의 것을 융합시키므로써 형태와 미학의 조화 속에 새로운 한국적 디자인을 창조하는 일이며, 미래의 가치를 찾는 작업이고, 새로운 전통 문화를 만드는 일이라고 할 수 있다. 본 연구는 우리 전통상징문양과 12지신 캐릭터에서 디자인 모티브를 발굴하고, 전통과 현대의 이미지를 접목하여 참신하고 세련된 감각과 흥미를 유발할 수 있는 캐릭터에 대한 표현연구를 통해 독특한 한국적 캐릭터를 개발하여 이를 현대 디자인에 다양하고 실질적인 활용방안을 제시하고자 했다. 이는 전통상징문양의 시각적인 효과와 캐릭터 개발만이 아닌, 우리만의 고유한 문화요소로 정체성과 독창성을 표현하여 외래문화 속에서 우리의 한국적 아이덴티티(Identity)를 찾고 한국적 디자인으로 미래의 세계시장에서 새로운 국가경쟁력을 갖기 위함이 그 목적이다.

• 한국가구학회지
• /
• 제20권5호
• /
• pp.504-516
• /
• 2009

Korean traditional architecture has been carried out in the manner that it harmonized surrounding natural environments and never disturbed them in the scale which did not overwhelm the nature, based on the view of architecture predicated on Yin Yang School. The exposed grain of the wood like pillar, hinge and house rafte, the linear expression of the construction material and the formation between the window and door monopolizing the front side presented a harmony with the nature showing the linear structure. Furthermore the ceiling is low due to the sit life style thanks to the ondol (Korean under-floor heating system and the furniture was made in simple manner to utilize the space as large as possible, for the interior was narrow and close, and also the furniture placing in the middle of the room was movably manufactured to improve the room space. Like this Korean traditional furniture was close associated with the low height and simplicity and harmonized with the blank of the wall accordingly, and it characterized linear & planar natural beauty focusing on the simple and refined unique beauty, because it was made in a good harmony with the interior utensils. This study has presented that the organic characteristics of Korean traditional aesthetic of architecture and the natural beauty being intrinsic in traditional furniture have a mutual relation on the basis of not only the visual recognition, also the aesthetic of naturalism made of the natural material, the constructional naivety as a frame method and the linear & planar formative beauty shown in appearance.

• Biomolecules & Therapeutics
• /
• 제27권2호
• /
• pp.160-167
• /
• 2019

Depression is a major mood disorder. Abnormal expression of glial glutamate transporter-1 (GLT-1) is associated with depression. Schisantherin B (STB) is one bioactive of lignans isolated from Schisandra chinensis (Turcz.) Baill which has been commonly used as a traditional herbal medicine for thousands of years. This paper was designed to investigate the effects of STB on depressive mice induced by forced swimming test (FST). Additionally, we also assessed the impairment of FST on cognitive function in mice with different ages. FST and open field test (OFT) were used for assessing depressive symptoms, and Y-maze was used for evaluating cognition processes. Our study showed that STB acting as an antidepressant, which increased GLT-1 levels by promoting PI3K/AKT/mTOR pathway. Although the damage is reversible, short-term learning and memory impairment caused by FST test is more serious in the aged mice, and STB also exerts cognition improvement ability in the meanwhile. Our findings suggested that STB might be a promising therapeutic agent of depression by regulating the GLT-1 restoration as well as activating PI3K/AKT/mTOR pathway.

• Journal of Ginseng Research
• /
• 제47권2호
• /
• pp.228-236
• /
• 2023

Background: QiShen YiQi pills (QSYQ) is a Traditional Chinese Medicine (TCM) formula, which has a significant effect on the treatment of patients with myocardial infarction (MI) in clinical practice. However, the molecular mechanism of QSYQ regulation pyroptosis after MI is still not fully known. Hence, this study was designed to reveal the mechanism of the active ingredient in QSYQ. Methods: Integrated approach of network pharmacology and molecular docking, were conducted to screen active components and corresponding common target genes of QSYQ in intervening pyroptosis after MI. Subsequently, STRING and Cytoscape were applied to construct a PPI network, and obtain candidate active compounds. Molecular docking was performed to verify the binding ability of candidate components to pyroptosis proteins and oxygen-glucose deprivation (OGD) induced cardiomyocytes injuries were applied to explore the protective effect and mechanism of the candidate drug. Results: Two drug-likeness compounds were preliminarily selected, and the binding capacity between Ginsenoside Rh2 (Rh2) and key target High Mobility Group Box 1 (HMGB1)was validated in the form of hydrogen bonding. 2 μM Rh2 prevented OGD-induced H9c2 death and reduced IL-18 and IL-1β levels, possibly by decreasing the activation of the NLRP3 inflammasome, inhibiting the expression of p12-caspase1, and attenuating the level of pyroptosis executive protein GSDMD-N. Conclusions: We propose that Rh2 of QSYQ can protect myocardial cells partially by ameliorating pyroptosis, which seems to have a new insight regarding the therapeutic potential for MI.

• 한국엔터테인먼트산업학회논문지
• /
• 제13권5호
• /
• pp.83-89
• /
• 2019

장예모 감독의 영화 ≪영≫은 중국전통문화상징을 다양하게 인용하여 시각적 효과를 높이는 반면, 스토리구성에 있어서는 대량의 현대문화상징의 내용을 인용하였다. 그러므로 그의 작품은 전통시각적인 느낌과 현대스토리내용을 모두 구비하였다. 의상과 장신구에서는 전통을 추구하였고 역사를 숭상하는데 충실하였으며 스토리구성에 있어서는 객관성 및 엄숙성을 담보하는 역사소설과 차별을 두었다고 할 수 있다. 가공문학해소로 역사를 새로 쓰고 더 나아가 역사에 대해 풀이함으로써 의도적으로 역사와 일정한 거리감 혹은 잘못된 관계를 유지하고 있다. 장예모 감독의≪영≫영화는 이러한 가공문학의 언사실천을 구사하였다. 영화는 역사를 시적으로 표현하는 한편 한 사람의 마음을 서사하였다. 예전의 영화제작과정에서 원작스토리에 충실하고자 했던 것에 반해 장예모의 ≪영≫은 (2018)제작 당시 원작 ≪삼국·정주≫의 작가 주수진의 동의하에 소설에 큰 변화를 주었다. 그 중에서도 제일 눈에 띄는 부분은 영화에서 원작의 설정을 과감하게 버리고 스토리의 시대배경을 허구적으로 구상해 냄으로써 가공문학을 완성시킨 것이다. 이는 결코 역사 문학의 언사실천은 아니다. 동시에 시각효과에 있어서 중국전통문화인 수묵 등 문화원소를 대량으로 인용하여 새로운 시각효과와 문화체험을 안겨주었다.

• 한국미생물·생명공학회지
• /
• 제20권6호
• /
• pp.637-641
• /
• 1992

한국 재래식 된장.간장 발효균 Bacillus sp. SSA3 균주의 expression vector를 개발하기 위해 Bacillus sp. SSA3의 chromosomal DNA로부터 유전자의 promoter 부위를 cloning 하였다. Recombinant plasmid를 제작하기 위하여 Bacillus sp. SSA3의 chromosomal DNA을 HindIII로 절단한 단편을 pGR71 plasmid의 CAT gene과 pUC18 plasmid의 $\beta$-galactosidase gene의 전방에 삽입시킨 후, E.coli JM109에 형질전환하였다.E. coli JM109의 chloramphenicol 내성 clones으로부터 6 recombinant plasmid를 선별하였다. 이들 선별된 plasmid는 Bacillus sp. SSA3의 expression vector로 사용시 각 재조합 plasmid 중에 삽입된 promoter의 염에 대한 영향의 정도를확인하기 위해 10% NaCl이 첨가된 LB medium상에서 배양하였을 때, 이들 중 Bacillus sp. SSA3의 4 clones은 융합 CAT gene의 발현이 강하게 감소되었으나 2 clones은 약하게 저해되었다.

• Journal of Microbiology and Biotechnology
• /
• 제25권3호
• /
• pp.343-352
• /
• 2015

H9 is an ethanol extract prepared from nine traditional/medicinal herbs. This study was focused on the anticancer effect of H9 in non-small-cell lung cancer cells. The effects of H9 on cell viability, apoptosis, mitochondrial membrane potential (MMP; ${\Delta}\psi_{m}$), and apoptosisrelated protein expression were investigated in A549 human lung cancer cells. In this study, H9-induced apoptosis was confirmed by propidium iodide staining, expression levels of mRNA were determined by reverse transcriptase polymerase chain reaction, protein expression levels were checked by western blot analysis, and MMP (${\Delta}\psi_{m}$) was measured by JC-1 staining. Our results indicated that H9 decreased the viability of A549 cells and induced cell morphological changes in a dose-dependent manner. H9 also altered expression levels of molecules involved in the intrinsic signaling pathway. H9 inhibited Bcl-xL expression, whereas Bax expression was enhanced and cytochrome C was released. Furthermore, H9 treatment led to the activation of caspase-3/caspase-9 and proteolytic cleavage of poly(ADP-ribose) polymerase; the MMP was collapsed by H9. However, the expression levels of extrinsic pathway molecules such as Fas/FasL, TRAIL/TRAIL-R, DR5, and Fas-associated death receptor were downregulated by H9. These results indicated that H9 inhibited proliferation and induced apoptosis by activating intrinsic pathways but not extrinsic pathways in human lung cancer cells. Our results suggest that H9 can be used as an alternative remedy for human non-small-cell lung cancer.

• 대한한의학회지
• /
• 제29권4호
• /
• pp.123-132
• /
• 2008

Objective: In this study, the author tried to investigate whether revised Seonbangpaedok-tang (SPT) and Sigyeongcheongpye-tang (SCT) significantly effects both PMA-induced mucin production and MUC5AC gene expression from airway epithelial cells. Objective: In this study, the author tried to investigate whether revised Seonbangpaedok-tang (SPT) and Sigyeongcheongpye-tang (SCT) significantly affects both PMA-induced mucin production and MUC5AC gene expression from airway epithelial cells. Materials and Methods: Confluent NCI-H292 cells were pretreated for 30 min in the presence of SPT and SCT and treated with PMA (10 ng/$m{\ell}$), to assess the effects of the agents on PMA-induced mucin production by enzyme-linked immunosorbent assay (ELISA). Also, the effects of the agents on PMA-induced MUC5AC gene expression from the same cells were investigated. Possible cytotoxicities of the agent were assessed by measuring the rate of survival and proliferation of NCI-H292 cells after treatment of agents during 48 hrs. Results: (1) SPT and SCT did not show significant cytotoxicity to NCI-H292 cells; (2) SPT significantly inhibitedthe expression levels of PMA-induced MUC5AC gene in NCI-H292 cells. SCT slightly decreased the expression levels of PMA-induced MUC5AC gene; (3) SPT significantly decreased PMA-induced mucin production from NCI-H292 cells. However, SCT did not affect mucin production. Conclusion: Theseresults suggest that SPT can not only affect the production of mucin but also the expression of the mucin gene and this explains the traditional use of SPT in oriental medicine. The effects of SPT and SCT with their components should be further investigated using animal experimental models that reflect pathophysiology of airway diseases via ongoing studies.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
• /
• pp.889-893
• /
• 2007

현재 다양한 연구에서는 유체인 안개의 자연스런 표현을 위해 프랙탈과 카오스 이론을 적용한다. 이러한 연구로 적용할 수 있는 전문 툴들을 사용하는 것은 자연스러운 표현이 가능하기 때문이다. 그러나, 일반인은 이러한 것을 아직 쉽게 표현하기는 매우 어려운데 그것은 고비용과 전문가들을 필요로 하기 때문이다. 따라서 누구나 어렵고 복잡한 고가의 전문툴들과 전문적으로 프로그래밍 제작을 최소로 한 안개의 표현을 쉽게 할수 있도록 하는 것이 일반적인 과제들이다. 본 연구는 이러한 2D 이미지에 대한 유체의 표현연구를 구체화하는 것으로 다양한 이미지 표현이 전문방송에서 개인에 이르는 UCC까지 가능하도록 쉽게 구현하였다. 특히, 이 연구는 3D 공간에서 안개의 유체적인 표현이 가능하기 때문에 한국화를 3D와 같이 표현할 때 배경에 안개의 표현을 쉽게 할 수 있도록 하였다.

• Advances in Traditional Medicine
• /
• 제7권5호
• /
• pp.556-563
• /
• 2008

Peripheral nerve injuries are a commonly encountered clinical problem and often result in a chronic pain and severe functional deficits. The expression of c-Fos is sometimes used as a marker of increased neuronal activity. We have prepared the aqueous extract of amygdalin from Armeniacae semen for pain control. In the present study, we investigated the effects of amygdalin on the recovery rate of the locomotor function and on the expression of c-Fos in the ventrolateral periaqueductal gray (vlPAG) region following sciatic crushed nerve injury in rats. Walking track analysis for the evaluation of functional recovery and immunohistochemistry for the c-Fos expression were used in this study. In the present results, characteristic gait change with dropping of the sciatic function index (SFI) was observed and c-Fos expression in the vlPAG was suppressed following sciatic crushed nerve injury in rats. Amygdalin enhanced SFI value and restored c-Fos expression in the vlPAG to the control value. The present our study indicated that amygdalin activates neurons in the vlPAG, and it facilitates functional recovery following peripheral nerve injury.