• 대한예방한의학회지
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• 제5권1호
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• pp.57-75
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• 2001

This study was conducted to improve the current regulation guidelines for developing traditional Korean medicines with effectiveness and productivity, in order to cope with the upcoming ICH on specifications on oriental herbal drugs. Also, major purposes of this study are to motivate R&D and to pioneer foreign markets for domestic herbal drug companies. First, after examining concepts of traditional Korean medicines and comparing the numbers and differentiation of herbal drugs registered on Pharmacopeia among Korea, China, and Japan, the current new drug development requirements for traditional Korean medicines were reviewed in detail, followed by comparison of foreign regulation systems including USA, EU, China, and Japan. Second, empirical cases on failure of development for new traditional Korean medicines under the current regulation system in the domestic companies including Dong-A, Kwangdong, and Samchondang, were collected and analyzed. As a result, hanbangsaengyak, the new category for traditional Korean medicines was newly developed on the basis of scientification of data between saengyak and hanyak, from the perspectives of harmonization between oriental medicine and western medicine and of balance between food and drug, in terms of industrialization, publicity, modernization, and effectiveness of administration. In addition, the new regulation requirements for the new hanbangsaengyak preparations were discussed by establishing principles of reinforcing preclinical test and of simplifying clinical trials in Korea. Finally, the further researches to articulate the complete specifications for pre-clinical and clinical requirements for traditional Korean medicines were strongly suggested.

• 한국한의학연구원논문집
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• 제10권1호
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• pp.81-95
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• 2004

The discussion of intellectual property protection with TK, GR in WIPO begin Herbal medicines get a important basis in a development of modern new medicine. For the protection of intellectual property, we set up this study. For the protection of intellectual property with herbal medicines, We analyze as follows. First, we analyze the specific theory and character of traditional herbal medicines. Second, we analyze the existing system on protecting them in Korea. The intellectual property protection with herbal medicines must maintain the specific character of that. We must select the basic traditional herbal medicines. The next, we must protect from intellectual property on add and subtract, combine, create of the traditional herbal medicines. In order that we must construct database on the Herbal medicines.

• 대한한의학회지
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• 제32권5호
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• pp.90-99
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• 2011

Objectives: Comparison of the status of herbal medicine management carried out by traditional Korean medicine hospitals and clinics, and changes in the prices of the herbal medicines offered by them. Methods: A questionnaire survey was conducted for 126 traditional Korean medicine hospitals and 4,200 traditional Korean medicine clinics. Questionnaire sheets were dispatched to them by mail in 2008. Rate of response: 57 hospitals (45.2%) and 465 clinics (11.0%) Results: 1. Concerning the herbal medicines kept by them, the hospitals and the clinics kept 295 and 147 kinds of medicine, respectively, on average. As for their monthly expenditure on the purchase of herbal medicines, the hospitals spent an average of KRW 28.38 million while the clinics spent an average of KRW 1.64 million. 2. The average monthly expenditure of the clinics on the purchase of herbal medicines decreased by 32% from 2006, which can be interpreted as a reduction of their business. 3. The prices of herbal medicines for disease treatment ranged from KRW 140,000~190,000 per jae(dosage form) in the hospitals compared to KRW 140,000~168,000 in the clinics. 4. Compared to 1994, the prices of herbal medicines used for disease treatment have risen, whereas the prices of herbal medicines used as tonics have decreased. Conclusions: There are no conspicuous differences between the hospitals and the clinics in terms of the prices charged for herbal medicines, regardless of whether they are used to treat diseases or prescribed as tonics.

• 생약학회지
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• 제47권1호
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• pp.12-17
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• 2016

In this study, the purification of the MeOH extract from the stems and leaves of Pourthiaea villosa var. brunnea using column chromatography furnished a main compound, lyoniside. The structure was elucidated on the basis of $^1H$ and $^{13}C$ NMR spectroscopic data. Quantitative analysis of lyoniside was conducted by HPLC method and the highest content of lyoniside was found in 50% MeOH reflux extraction. To investigate the anti-inflammatory effect of the lyoniside, we measured nitric oxide and pro-inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, IL-6 levels in lipopolysaccharide-induced murine macrophage cell line RAW 264.7. As a results, lyoniside decreased the level of nitric oxide and IL-6 in concentration dose dependent manner in RAW 264.7 cells.

• Journal of Ginseng Research
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• 제43권1호
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• pp.116-124
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• 2019

Background: Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such as Asian ginseng, American ginseng, and Notoginseng. Some ginsenosides, especially the 20(R) isomers, are found in trace amounts in natural sources and are difficult to chemically synthesize. The present study provides an approach to produce such trace ginsenosides applying biotransformation through Escherichia coli modified with relevant genes. Methods: Seven uridine diphosphate glycosyltransferase (UGT) genes originating from Panax notoginseng, Medicago sativa, and Bacillus subtilis were synthesized or cloned and constructed into pETM6, an ePathBrick vector, which were then introduced into E. coli BL21star (DE3) separately. 20(R)-Protopanaxadiol (PPD), 20(R)-protopanaxatriol (PPT), and 20(R)-type ginsenosides were used as substrates for biotransformation with recombinant E. coli modified with those UGT genes. Results: E. coli engineered with $GT95^{syn}$ selectively transfers a glucose moiety to the C20 hydroxyl of 20(R)-PPD and 20(R)-PPT to produce 20(R)-CK and 20(R)-F1, respectively. GTK1- and GTC1-modified E. coli glycosylated the C3-OH of 20(R)-PPD to form 20(R)-Rh2. Moreover, E. coli containing $p2GT95^{syn}K1$, a recreated two-step glycosylation pathway via the ePathBrich, implemented the successive glycosylation at C20-OH and C3-OH of 20(R)-PPD and yielded 20(R)-F2 in the biotransformation broth. Conclusion: This study demonstrates that rare 20(R)-ginsenosides can be produced through E. coli engineered with UTG genes.

• Journal of Ginseng Research
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• 제44권3호
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• pp.442-452
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• 2020

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioning at molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegeneration in the central nervous system. This study is aimed to explore the alleviative effect and the underlying molecular mechanism of stem-leaf saponins of Panax notoginseng (SLSP) on the abnormal neuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory induced by SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronal morphological changes were observed by Nissl staining. Autophagosome formation was examined by transmission electron microscopy, immunofluorescent staining, acridine orange staining, and transient transfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin V staining. The expression or activation of autophagy and apoptosis-related proteins were detected by Western blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h, accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampal neurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycin and re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transduction in HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probably through suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings may contribute to the clinical application of SLSP in the prevention or therapy of neurological disorders associated with SD.

• 한국한의학연구원논문집
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• 제17권2호
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• pp.17-28
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• 2011

This study was conducted to analyze the status of the traditional medicines registered by the Chinese state as intangible cultural heritages, and of Chinese acupuncture and moxibustion registered by UNESCO as world intangible cultural heritages in 2010, in connection with the need to have Traditional Korean Medicine skills similarly registered as world intangible cultural heritage items. Since UNESCO's adoption of the Convention on the Safeguarding of Intangible Cultural Heritages in 2003, the Chinese government has pushed ahead with efforts to explore and protect intangible cultural heritage items and have them registered as world intangible cultural heritages. Thus, Benchaogangmu (本草綱目) and Huangdineijing (黃帝內經) were listed on UNESCO's Memory of the World Register while Chinese acupuncture and moxibustion were registered as world intangible cultural heritage properties. For Traditional Korean Medicine skills to be listed as world intangible cultural heritage properties, steps should be taken first to include the skills in the list of domestic intangible cultural heritage items and to make further explorations in the sector. Such efforts are also important in terms of the need to establish the legitimacy of Traditional Korean Medicines.

• 대한예방한의학회지
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• 제15권1호
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• pp.1-16
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• 2011

Objectives : The mechanisms for korean traditional medicine-drug interaction has not been well reviewed in spite that the chance for co-administration with western drugs or diet supplements has been increased. Especially, it is well known that various cytochrome P450s play a major role in drug-drug interaction. Of course, Korean traditional medicines is not excluded in a view of metabolism or biotransformation by cytochrome P450. This article was focused on reviewing the possible roles of cytochrome P450 in Korean traditional medicine-drug interaction, Also, the directions for further studies were suggested in terms of Korean traditional medicine-drug interaction. Methods : New studies for korean traditional medicine-drug interaction were reviewed and summarized in terms of cytochrome P450 activities by various Korean traditional medicines and western drugs. Results and Conclusions : Even if a few studies related to Korean traditional medicine-drug interactions was carried out, almost no studies for Korean traditional medicine-drug interactions has been found in a view of cytochrome P450. It was suggested that Korean traditional medicines and their decoction should be analyzed that how they effects on cytochrome P450, expecially CYP 1, 2, 3 families and how they interact with western drugs.

• 동의생리병리학회지
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• 제18권5호
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• pp.1387-1390
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• 2004

This study was carried out to investigate the solubility of the ingredients of the bilestone in the solution of the traditional oriental medicines. The cholesterol and the calcium carbonate(CaCO₃) were chosen as the ingredients of the bilestone. Coicis Semen, Polygonum aviculare L, Maydis Stigmata, Allium tuberosum Rottler, and Raphanus sativa var. hortensis for. acanthiformis Makino were studied as the oriental medicines for the bilestone. The cholesterol had showed no solubility in all solutions of the traditional oriental medicines, but the calcium carbonate had showed the good solubility in all solutions of the traditional oriental medicines except Coicis Semen.

• 생약학회지
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• 제44권1호
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• pp.1-5
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• 2013

Nelnumbo nucifera Gaerth. (Nymphaeaceae) has been used in a korean traditional medicine to treat fever, sunstroke and dizziness. The receptaculums of this plant were refluxed with MeOH, and then fractionated with organic solvents ($CH_2Cl_2$, EtOAc and n-BuOH) to screen whitening activity using tyrosinase inhibitory activity. EtOAc ($IC_{50}$, 45.23 ${\mu}g/ml$) fractions showed a good tyrosinase inhibitory activity. Column chromatographic separation of $CH_2Cl_2$ and EtOAc fractions of Receptaculum nelumbinis led to the isolation 3 compounds. Their chemical structures were characterized as ${\beta}$-sitosterol (1), quercetin 3-O-${\beta}$-D-galactopyranoside (2) and kaempferol 3-O-${\beta}$-D-glucopyranoside (3) by comparison NMR spectral data and with those in references, respectively. Isolated compounds 1 and 3 were firstly isolated from Receptaculums nelumbinis. Compounds 2 and 3 showed potent whitening activities.