Isolation of Compounds having Inhibitory Activity toward Tyrosinase from Receptaculum Nelumbinis

연방(蓮房)의 티로시나제 저해 활성을 보이는 성분분리

  • Cho, Hyun Woo (Jeollanamdo Development Institute for Korean Traditional Medicines) ;
  • Jung, Won Seok (Jeollanamdo Development Institute for Korean Traditional Medicines) ;
  • An, Byeong Gwan (Jeollanamdo Development Institute for Korean Traditional Medicines) ;
  • Cho, Jung Hee (Jeollanamdo Development Institute for Korean Traditional Medicines) ;
  • Jung, Su Young (Jeollanamdo Development Institute for Korean Traditional Medicines)
  • 조현우 (전라남도생물산업진흥재단 한방산업진흥원) ;
  • 정원석 (전라남도생물산업진흥재단 한방산업진흥원) ;
  • 안병관 (전라남도생물산업진흥재단 한방산업진흥원) ;
  • 조정희 (전라남도생물산업진흥재단 한방산업진흥원) ;
  • 정수영 (전라남도생물산업진흥재단 한방산업진흥원)
  • Received : 2012.10.11
  • Accepted : 2013.01.11
  • Published : 2013.03.31

Abstract

Nelnumbo nucifera Gaerth. (Nymphaeaceae) has been used in a korean traditional medicine to treat fever, sunstroke and dizziness. The receptaculums of this plant were refluxed with MeOH, and then fractionated with organic solvents ($CH_2Cl_2$, EtOAc and n-BuOH) to screen whitening activity using tyrosinase inhibitory activity. EtOAc ($IC_{50}$, 45.23 ${\mu}g/ml$) fractions showed a good tyrosinase inhibitory activity. Column chromatographic separation of $CH_2Cl_2$ and EtOAc fractions of Receptaculum nelumbinis led to the isolation 3 compounds. Their chemical structures were characterized as ${\beta}$-sitosterol (1), quercetin 3-O-${\beta}$-D-galactopyranoside (2) and kaempferol 3-O-${\beta}$-D-glucopyranoside (3) by comparison NMR spectral data and with those in references, respectively. Isolated compounds 1 and 3 were firstly isolated from Receptaculums nelumbinis. Compounds 2 and 3 showed potent whitening activities.

Keywords

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