• Journal of Nutrition and Health
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• v.36 no.7
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• pp.729-735
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• 2003

This study was performed to evaluate the effect of iron supplement for 4 weeks on iron status, immunity, and antioxidant status of national female soccer players (n = 25). This study was performed at summer hard training period right before competition. A single blind design was used to divide the subjects into iron-supplement (IS) or placebo group (P). Iron-supplement group was supplemented with iron (40 mg/d) for 4 weeks. The mean age of the subjects was 23.3 $\pm$ 2.5 years old. Mean height and body weight of the subjects were 164.4 $\pm$ 5.7 em and 57.4 $\pm$ 4.6 kg, respectively. The mean carrier as soccer player was 11.0 $\pm$ 2.6 years and mean training time was 7.0 $\pm$ 1.3 hr/day. The mean hemoglobin, hematocrit, total iron binding capacity and ferritin concentrations before iron supplementation were not different between two groups. After 4 weeks of summer training and iron supplementation, serum ferritin level was significantly increased only in IS group after supplementation. Mean corpuscular volume and total iron binding capacity were significantly decreased in both groups. Meanwhile, hemoglobin and red blood cell count were significantly lowered only in placebo group. The IgM concentration increased significantly in both groups, but IgG concentration had increasing tendency only in IS group (p < 0.064). Therefore, iron supplementation during hard training period may be helpful to improve work capacity of the athletes by improving ferritin status and humoral immune responses.

• Earthquakes and Structures
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• v.14 no.5
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• pp.437-447
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• 2018

Computational cost is one of the major obstacles for detailed risk analysis of structures. This paper puts forward a methodology for efficient probabilistic seismic loss assessment of structures using the Endurance Time (ET) analysis and the first-order reliability method (FORM). The ET analysis efficiently yields the structural responses for a continuous range of intensities through a single response-history analysis. Taking advantage of this property of ET, FORM is employed to estimate the annual rate of exceedance for the loss components. The proposed approach is an amalgamation of two analysis approaches, ET and FORM, that significantly lower the computational costs. This makes it possible to evaluate the seismic risk of complex systems. The probability distribution of losses due to the structural and non-structural damage as well as injuries and fatalities of a prototype structure are estimated using the proposed methodology. This methodology is an alternative to the prevalent risk analysis framework of the total probability theorem. Hence, the risk estimates of the proposed approach are compared with those from the total probability theorem as a benchmark. The results indicate a satisfactory agreement between the two methods while a significantly lower computational demand for the proposed approach.

• Journal of the Korean Applied Science and Technology
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• v.21 no.2
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• pp.148-155
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• 2004

Response surface analysis was used to study dietary ratios of n-3/n-6 fatty acid and P/S to minimize plasma triglycerides, total cholesterol and LDL ${\cdot}$ VLDL-C levels and maximize plasma HDL ${\cdot}$ C levels of rats. Because the dietary components were not statistically independent, they were studied in combinations of two variables. The two-variable combinations were the most useful in locating the desired maximum or minimum plasma triglycerides, total cholesterol and LDL ${\cdot}$ VLDL-C response in terms of the proportions of the dietary components. Response surface contours and three dimensional plots were developed for each plasma lipid response. The contours and three dimensional plots were used to help determine those combinations of the dietary fatty acid ratios that would produce the desired minimum or maximum lpid responses. The statistical analyses indicated that the minimized plasma cholesterol response levels could be attained with a diet consisting of 2.26 n-3/n-6 fatty acid and 2.15 P/S ratios.

• Journal of Microbiology and Biotechnology
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• v.27 no.8
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• pp.1519-1528
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• 2017

Foodborne contamination and salmonellosis caused by Salmonella Enteritidis (S. Enteritidis) are a significant threat to human health and poultry enterprises. Outer membrane vesicles (OMVs), which are naturally secreted by gram-negative bacteria, could be a good vaccine option because they have many biologically active substances, including lipopolysaccharides (LPS), outer membrane proteins (OMPs), and phospholipids, as well as periplasmic components. In the present study, we purified OMVs derived from S. Enteritidis and analyzed their characteristics through silver staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis. In total, 108 proteins were identified in S. Enteritidis OMVs through liquid chromatography tandem mass spectrometry analysis, and OMPs, periplasmic proteins, and extracellular proteins (49.9% of total proteins) were found to be enriched in the OMVs compared with bacterial cells. Furthermore, native OMVs used in immunizations by either the intranasal route or the intraperitoneal route could elicit significant humoral and mucosal immune responses and provide strong protective efficiency against a lethal dose (~100-fold $LD_{50}$) of the wild-type S. Enteritidis infection. These results indicated that S. Enteritidis OMVs might be an ideal vaccine strategy for preventing S. Enteritidis diseases.

• Journal of Environmental Science International
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• v.20 no.1
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• pp.93-106
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• 2011

Physically-based resampling scheme for roughness coefficient of surface runoff considering the spatial landuse distribution was suggested for the purpose of effective operational application of recent grid-based distributed rainfall runoff model. Generally grid scale(mother scale) of hydrologic modeling can be greater than the scale (child scale) of original GIS thematic digital map when the objective basin is wide or topographically simple, so the modeler uses large grid scale. The resampled roughness coefficient was estimated and compared using 3 different schemes of Predominant, Composite and Mosaic approaches and total runoff volume and peak streamflow were computed through distributed rainfall-runoff model. For quantitative assessment of biases between computational simulation and observation, runoff responses for the roughness estimated using the 3 different schemes were evaluated using MAPE(Mean Areal Percentage Error), RMSE(Root-Mean Squared Error), and COE(Coefficient of Efficiency). As a result, in the case of 500m scale Mosaic resampling for the natural and urban basin, the distribution of surface runoff roughness coefficient shows biggest difference from that of original scale but surface runoff simulation shows smallest, especially in peakflow rather than total runoff volume.

• Journal of Home Health Care Nursing
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• v.17 no.1
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• pp.55-62
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• 2010

Purpose: This study investigated the sought to identify the pain management knowledge and attitude of nurses in internal medicine and surgery stream wards and intensive care units to obtain basic information needed for improved pain control. Method: Data was collected through self-reported questionnaires and analyzed by descriptive statistics, t-test and ANOVA using SPSS Win 12.0. Results: Pain management knowledge score averaged 51.1 out of 100, with significant differences evident depending on age, marital status, educational level, position, total work career, working department and cancer care-giving experience. Pain management attitude score averaged 2.9 out of 4, and significant differences were evident depending on age, marital status, educational level, position, total work career, working department, cancer care-giving experience, education experience and number of patients treated. Conclusion: Further studies are necessary to develop effective nursing knowledge and attitude education programs and evaluation such as discussion by cases, workshops, system of expert help for uncontrollable pain or a multi-disciplinary pain management task force. In addition, patient satisfaction level with pain management should be assessed and the responses reflected practically.

• Fashion & Textile Research Journal
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• v.18 no.6
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• pp.833-843
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• 2016

Due to the rapid expansion of fashion, consumers easily purchase fashion products, the period of wearing apparel is shortened, and the occurrence of clothes piling up in the closet is increasing. In order to induce and suggest rational consumption and disposal actions, research focused on the factors influencing difficulty discarding and disposal behavior toward a fashion product is needed. Thus, this study considered fashion-shopping orientation as a variable affecting difficulty discarding and disposal behavior toward fashion products. A total of 325 questionnaires were collected, and 11 were discarded due to partial responses or missing data. Finally, a total of 314 survey questionnaires were analyzed. Frequency, exploratory factor, reliability, and multiple regression analyses were employed for data analysis using SPSS 23.0. The study results were as follows. First, hedonic and economic shopping orientation positively affected difficulty discarding a fashion product, whereas rational shopping orientation negatively affected difficulty discarding a fashion product. Second, hedonic, economic, and conspicuous shopping orientation positively affected reuse behavior among disposal behavior toward a fashion product. Third, trend-seeking and convenient shopping orientation positively influenced handover behavior. Fourth, economic and conspicuous shopping orientation positively affected separation discard behavior. The results of this study provide various guidelines for manufacturers and retailers of fashion products.

• Journal of Microbiology and Biotechnology
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• v.5 no.4
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• pp.181-187
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• 1995

The cells of Vibrio vulnificus can be induced to the viable but nonculturable (VBNC) state by natural environmental parameters. The V. vulnificus cells in the VBNC state can not be recovered by ordinary laboratory techniques. This nonculturability could often hamper development of effective processing strategies to minimize the number of V. vulnificus in seafoods. Even with V. vulnificus cells in a culturable state, the length of time required to identify the bacteria in contaminated food by phenotyphic characterization may prevent appropriate in-time responses by public health agencies to infections of the bacteria. In the present study, we used polymerase chain reaction (PCR) to develop a rapid and direct detection method for V. vulnificus in small octopus (Octopus variabilis) which is consumed as a raw food in Korea. The region targeted was a 704-base pair (bp) portion of the hemolysin gene, vvhA, of V. vulnificus. The primers designed for PCR amplification were specific for all V. vulnificus sp. tested. Several methods were examined to extract total DNA directly from V. vulnificus seeded into the octopus homogenate and the guanidine isothiocyanate (CITC) method appeared to be most effective. From the octopus homogenate seeded by V. vulnificus at an initial level of $10^2$ CFU/ml of the homogenate and then incubated for 12 h, the targeted sequence was successfully amplified by PCR and the 704-bp DNA fragment was observed by gel electrophoresis. The total completion of this assay requires less than one day.

• Journal of Embryo Transfer
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• v.9 no.2
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• pp.189-196
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• 1994

This study was perfomed to investigate the differentiation of rabbit blastocysts microinjected with testosterone solution. A total of 140 mixed breed does was superovulated, synchronized and hand mated. The eggs were flushed from uterine horns between 65 and 89 hrs after mating. Testosterone was dissolved in 95% ethanol and diluted with PBS at the ratio of 1: 99. Final concentration of testosterone was adjusted to 1 pg /ml. 6~8 bias-tocysts were microinjected with 1~10 p Q of the diluted testosterone solution, and tranfer-red into the uterine horns of the synchronized recipients. When 140 donor does were treat-ed with a single does of 200 IU PMSG in combination with 100 IU RCG 48 hrs apart, 134 of them(97%) showed standing estrus. Ovarian responses of 117 does were examined following mating and the rate of ovulation was 11.23 i 1.20. Ova were recovered from donors between 65 and 89 hrs after mating. Recovery rates of ova were 37.5% and 42.2% of recovered ova were blastocysts. A total of 106 blastsocysts were microinjected with testosterone solution and transferred into the uterine horns of 15 synchronized recipient does. One of the recipients was pregnant and delivered 7 baby rabbits. The external genitalia of the young rabbits appered to be the same appearance as the buck entierly.

• Journal of the Korean housing association
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• v.27 no.4
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• pp.67-76
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• 2016

The purpose of the study was to identify user' satisfaction about universal design in public service center that will be needed for helping planning and design the public service center. This study conducted a questionnaire survey targeting visitor in five district's public service center in Gwangju metropolitan city. A total of 253 responses were analyzed for identifying the level of satisfaction about universal design in public service center. The study also analyzed of the respondents depending on their demographic characteristics. The features of universal design were categorized into supportive design, communicability, safety-oriented design and accessible design, and total 25 specific items were included in the evaluation. Most of the respondents were satisfied with the waiting space, however they showed a lower level of satisfaction toward the information materials space. Those with a higher education attainment tended to be associated with stronger satisfaction with common space and information materials space. Groups with a lower age were more likely to be satisfied with common space and public service space. Also female tended to show a stronger degree of satisfaction with common space than male. The findings from this study should provide a guideline for planning and design the public service center.