• Korean Journal of Veterinary Research
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• v.37 no.3
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• pp.583-593
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• 1997

Indirect fluorescent antibody test(IFAT) and enzyme-linked imuunosorbent assay (IgG-ELISA) as serological diagnostic tools were conducted to evaluate the usefulness for diagnosis of canine babesiosis infected with Babesia gibsoni in domestic various dog breeds, american pit bullterrier, military shepherd, and mongrel dogs. The results obtained from this study were abstracted as follows. The nonionic detergent Triton X-100 and absorbent bio-bead $SM_2$ were useful reagents for the preparation of pure merozoite antigen of B gibsoni to be used in ELISA. The optimum reaction in ELISA was shown when the protein concentration of ELISA antigen was measured as 625ng/ml and the conjugate concentration was diluted into 1/6000 fold. The average OD value of ELISA in sera determined with negative responses in IFAT was measured as $0.255{\pm}0.051$(490nm) and the cut - off value of OD was determined as 0.399(490nm). The serum antibodies in both of IFAT and ELISA were detected on one week after artificially infected with B gibsoni and these high antibody titers, 512X in IFAT and 1024X in ELISA, were long lasted until 15 weeks after infection. The reproducibility of reaction and stability of the antigen absorbed microtitration polystyrene plate preserved in $4^{\circ}C$ refrigerator and $-20^{\circ}C$ freezer, respectively could be lasted until 135 days after storage. The positive rates in IFAT by dog breeds were shown 8.1%(60/744 heads) in mongrel dogs, 81.3%(78/96 heads) in american pit bullterrier and 15.6%(15/96 heads) in military shepherd, while the positive rate in ELISA shown 17.6%(131/744 heads) in mongrel dogs, 83.3%(80/96 heads) in american pit bullterrier and 36.5%(35/96 heads) in military shepherd, respiectively. In the total of 936 heads surveyed with IFAT and ELISA the positive rates in IFAT and ELISA were 16.4%(153/936 heads) and 26.3%(246/936 heads), respectivily. Agreement of reactions between IFAT and ELISA was shown 82.4% in 936 dog sera. The specificity and sensitivity of ELISA reaction were 83.5% and 76.5%, respectively. From the conclusion obtained in this study it was evaluated that IFAT and ELISA were useful as highly specific, sensitive and stable serelogical tools for the diagnosis of canine babesiosis in Korea.

• Journal of Life Science
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• v.29 no.12
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• pp.1401-1407
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• 2019

To elucidate whether or not solvent-tolerant bacteria use anti-oxidative defense systems to defend themselves against toxic solvents, oxidative enzyme activity and total anti-oxidative capacity (T-AOC) were investigated in two tolerant strains of Pseudomonas sp. under toluene stress. The superoxide dismutase (SOD) activities of solvent tolerant BCNU 106 exhibited relatively increased levels at a toluene concentration of 100 mg/l, where those of solvent tolerant BCNU 171 increased at 200 mg/l. A greater than three-fold increase in catalase (CAT) levels was observed at concentrations of 200 and 300 mg/l in BCNU 106, and a two-fold increase was monitored at the same concentrations in BCNU 171. High glutathione S-transferase (GST) levels were also observed in the solvent tolerant bacteria. Higher levels of T-AOC was expressed in the solvent tolerant strains than in the ordinary non-tolerant KACC 10266. The highest plateau of SOD in BCNU 171 was observed at 1 hr of toluene exposure. CAT levels plateaued at 1 hr and 14 hr in BCNU 106 and reached the highest plateau at 3 hr in BCNU 171. The highest peak of T-AOC occurred at 9 hr in BCNU 106, and two high peaks occurred in BCNU 171, at 1 hr and at 9 hr of toluene exposure. The solvent-tolerant bacteria showed active antioxidant responses and could survive under harsh environments, including the presence of solvents, through means of antioxidant defense systems.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.1
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• pp.33-44
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• 2000

Ships in a seaway will encounter dangerous situation, such as slamming, stranding, and capsizing. The number of capsizing is small, but the loss due to them is very large from the viewpoint of human life, property, and the environmental pollution. The number of capsizing of fishing vessels is about 62% of total number of capsizing, and the half of them is originated from the operational mistake in a seaway. So the dynamics and the capsizing phenomena are to be studied, and the guide for the safe operation of a fishing vessel in a seaway are to be specified. The hydrodynamic forces consist of radiation forces (which are due to the motion of a ship), Froude-Krylov forces (which is due to the incoming waves), and diffraction forces (which is due to the wave and ship interaction). These forces are calculated by well-known strip method. Using the calculated forces, the motion of a ship in a regular sea is obtained. In the real seaway, the waves are very irregular, therefore the statistical analysis is very helpful. In this paper, using the results of the motion in a regular seaway and the wave spectrum, the motion in a irregular seaway are obtained and analyzed.

• Korean Journal of Agricultural and Forest Meteorology
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• v.10 no.2
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• pp.47-57
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• 2008

Physiological and biochemical changes of five species of genus Quercus exposed to ozone fumigation were investigated to assess their tolerance against ozone toxicity. At the end of 150 ppb ozone fumigation, chlorophyll contents, photosynthetic characteristics, malondialdehyde(MDA) and antioxidative enzyme activities were measured in the leaves of five Quercus species(Quercus acutissima, Q. aliena, Q. palustris, Q. serrata, and Q. variabilis). Chlorophyll and carotenoid contents, net photosynthesis and carboxylation efficiency decreased after ozone treatment, indicating that $O_3$-exposed plants underwent physiological inhibition. The reduction rate of total chlorophyll contents and carboxylation efficiency were respectively 15% and 34% for Q. aliena and 38% and 62% for Q. variabilis. The amount of MDA increased with the highest increase rate of 140% in Q. acutissima which also showed the highest increase rate(60%) of superoxide dismutase(SOD). Ascorbate peroxidase(APX) activity increased in Q. variabilis, Q. serrata and Q. acutissima by ozone treatment. Based on our results, ozone tolerance of the five Quercus species was ranked as Q. aliena>Q. palustris>Q. serrata>Q. variabilis>Q. acutissima. We concluded that chlorophyll contents, photosynthesis, MDA content and antioxidative enzymes were the important physiological markers for tolerance against ozone stress, which were closely related with one another.

• Journal of Veterinary Clinics
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• v.32 no.2
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• pp.174-179
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• 2015

Academic integrity guarantees the professional integrity and validity of the education and qualifications offered by the veterinary schools. In this study, we analyzed the responses of 528 veterinary students of two veterinary schools in Seoul regarding their awareness about, knowledge of, and frequency of engaging in academic misconduct. A total of 88.4 percent of the participants agreed that cheating and plagiarism by undergraduates would influence their future academic misconduct. The most common form of academic misconduct was plagiarism (71.7% in the A school, 69.5% in the B school), with falsification (40.2% in the A school, 31.7% in the B school) also reported at a high rate. Students indicated the lack of a culture of academic integrity as the main reason for academic misconduct. According to the regression analysis students' awareness and knowledge of academic integrity and their perception of peers' academic misconduct predicted a significant amount of variance of the frequency of academic misconduct. The findings of this study support that academic integrity should be learned in a flexible format from an early stage of professional development in veterinary curriculum. In parallel with the efforts of faculty, a community approach may be likely to improve the academic environment in terms of integrity.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.26 no.1
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• pp.58-63
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• 2016

Objectives: One major limitation encountered in retrospective exposure assessment for epidemiological study is the lack of exposure records and information maintained by companies which if they existed would allow the estimation of past exposure to hazardous operations and agents. This study developed a job-specific questionnaire(JSQ) to estimate exposure profiles among semiconductor workers, including operation and job. Methods: This JSQ can be directly applied to workers who work or have worked in a wafer fabrication or a chip packaging and assembly facility. Results and Conclusions: We used this JSQ to obtain past exposure information from semiconductor workers via face-to-face investigation. Major contents include questions on the facilities, operations and jobs to which they have been exposed since they entered employment in the semiconductor industry. The total number of questions in the JSQ is 18. Responses to this JSQ can be used not only to estimate retrospective exposure to operations and jobs in the semiconductor industry, but also to associate with the risk of all causes of death and risk of disease, including cancer.

• Journal of Wetlands Research
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• v.16 no.4
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• pp.363-370
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• 2014

We grew seedlings of Saechucheongbyeo and Odaebyeo of rice cultivars that are cultivated dominantly in the northern and middle regions of Korea under control(ambient condition), ambient $CO_2$ concentration+elevated temperature, and elevated $CO_2$ concentration+elevated temperature in order to study how growth responses and crop yield of major rice of Korea change as the global warming proceeds and compared the results. Aboveground biomass, belowground biomass, total biomass, and panicles weight per individual and ripended grain rate of cv. Saechucheongbyeo were the highest under control, but those of cv. Odaebyeo were the highest under elevated $CO_2$ concentration+elevated temperature. There was no difference in the number of panicles per individual of cv. Saechucheongbyeo and cv. Odaebyeo in these experiments. There was no difference in the number of grains per panicle of cv. Saechucheongbyeo among three environmental gradients, but that of cv. Odaebyeo was the highest under elevated $CO_2$ concentration+elevated temperature. Weight of a grain of cv. Saechucheongbyeo was highest under elevated $CO_2$ concentration+elevated temperature, but that of cv. Odaebyeo was the higher under ambient $CO_2$ concentration+elevated temperature and elevated $CO_2$ concentration+elevated temperature. Thus, if global warming continues in Korea, selection of rice cultivation varieties must be chosen carefully for commendation.

• Molecular & Cellular Toxicology
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• v.3 no.2
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• pp.98-106
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• 2007

Genotoxic stress triggers a variety of biological responses including the transcriptional activation of genes regulating DNA repair, cell survival and cell death. In this study, we investigated to examine gene expression profiles and genotoxic response in TK6 cells treated with DNA damaging agents MNNG (N-methyl-N'-nitrosoguanidine) and hydrogen peroxide $(H_2O_2)$. We extracted total RNA in three independent experiments and hybridized cRNA probes with oligo DNA chip (Applied Biosystems Human Genome Survey Microarray). We analyzed raw signal data with R program and AVADIS software and identified a number of deregulated genes with more than 1.5 log-scale fold change and statistical significancy. We indentified 14 genes including G protein alpha 12 showing deregulation by MNNG. The deregulated genes by MNNG represent the biological pathway regarding MAP kinase signaling pathway. Hydrogen peroxide altered 188 genes including sulfiredoxins. These results show that MNNG and $H_2O_2$ have both uniquely regulated genes that provide the potential to serve as biomarkers of exposure to DNA damaging agents.

• Journal of the Korean Society of Clothing and Textiles
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• v.16 no.3 s.43
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• pp.181-195
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• 1992

The purposes of this study were to investigate 1) the effect of body exposure and color of a woman's suit on the perception of modesty, and 2) the effect of perceiver's sex and age on impression formed by the function of clothing variables. The instrument of this study consisted of a response scale and stimuli. Thirteen items of 7-point semantic differential scales were developed to measure the perceiver's impression on wearer's modesty. Stimuli were color pictures of a model wearing one of 8 types of suit constructed by a 2 $\times$ 2 $\times$ 2 factorial design. The manipulation of each level of the clothing variables were: color of the suit by black and red, leg exposure by varying skirt lengths to a Chanel-line and mini skirt, and neck exposure by shirt collar blouse and scarf. Two models, representing typical female college students living in Seoul, were selected to eliminate model effect. The sample include 384 subjects, consisting of 4 groups of male and female college students and middle aged men and women. Eight experimental groups were randomly assigned to one of eight stimuli based on between-subject design. One half of each group responded to model 1 and the other half to model 2 of same stimulus. Responses to the semantic differential scales were factor analyzed (pc model, Varimax rotation) to identify factors constructing impression of modesty. Two factors emerged regard­less of subgroups; Elegance and Extroversion factor. The first factor was found to be dominant, accounting for 60 percent of the total variance. The other accounted for just 11 percent. Multidimensional ANOVA (5-way, 3-way) was conducted to test the effect of the clothing variables against two factors identified from the factor analysis. Leg exposure was the most powerful variable affecting the impression of Elegance and Extroversion factor for all per. ceiver subgroups. Neck exposure had primary effect on the impression of Elegance, whereas it partially influenced that of Extroversion. Color of suit had only partial effect on the impression of Extroversion. Hypothesis I was partially supported from the findings above. The effect of perceiver's age and sex on impression by the function of clothing variables was tested by comparing the result between four subgroups. In forming an impression of the wearer's modesty, male college students were least affected by the manipulation of clothing variables, while middle aged males were affected most. In the female groups, there was no age difference and they fell between the male groups in the degree to which they were affected. Hypothesis II was supported only by age difference in two male groups, and by sex difference in two student groups.

• Applied Microscopy
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• v.27 no.1
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• pp.71-86
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• 1997

The regeneration and differentiation of the cutaneous pigment system in the goldfish, Carassius auratus during the wound healing process were studied with high magnification electron microscope. The cutaneous pigment cells of the normal tissues were composed of three kinds of dermal chromatophores-xanthophores, leucoiphores and melanophores. While xanthophores contain two kinds of pigment granules-pterinosomes and carotenoid vesicles, leucophores and melanophores contain amorphous pigment granules (leucosomes) and oval shaped electron dense melanin pigment granules (melanosomes) respectively. After injury, primary wound healing responses being carried out by migration of epidermal cells and hemocytes spreading over the wound surface at the day of wounding. And at the time of primary wound closure, 5 to 7 days after wounding, rER rich cells-presumably common precursors of dermal chromatophores-immigrated into the wound area. First redifferentiated chromatophores appeared 3 weeks after wounding. Pigment granules of the chromatophores were emerged from the cytoplasmic Golgi complex via rough endoplasmic reticulum. Pinocytotic vesicles which associated with accumulation of pigment material, appeared only at the inner surface of the chromatophores adhering to the rER rich cells, characteristically. The differentiation of each chromatophore in addition to integumental wound repair were accomplished within 4 weeks after wounding at most cases, however the total numbers and densities of these repaired chromatophores still primitive state. Moreover, It has been revealed that complete repair of chromatophores at wounded tissues from burns requirs more than 3 months in normal environment.