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Transcriptome-wide analysis reveals gluten-induced suppression of small intestine development in young chickens

  • Darae, Kang;Donghyun, Shin;Hosung, Choe;Doyon, Hwang;Andrew Wange, Bugenyi;Chong-Sam, Na;Hak-Kyo, Lee;Jaeyoung, Heo;Kwanseob, Shim
    • Journal of Animal Science and Technology
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    • v.64 no.4
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    • pp.752-769
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    • 2022
  • Wheat gluten is an increasingly common ingredient in poultry diets but its impact on the small intestine in chicken is not fully understood. This study aimed to identify effects of high-gluten diets on chicken small intestines and the variation of their associated transcriptional responses by age. A total of 120 broilers (Ross Strain) were used to perform two animal experiments consisting of two gluten inclusion levels (0% or 25%) by bird's age (1 week or 4 weeks). Transcriptomics and histochemical techniques were employed to study the effect of gluten on their duodenal mucosa using randomly selected 12 broilers (3 chicks per group). A reduction in feed intake and body weight gain was found in the broilers fed a high-gluten containing diet at both ages. Histochemical photomicrographs showed a reduced villus height to crypt depth ratio in the duodenum of gluten-fed broilers at 1 week. We found mainly a significant effect on the gene expression of duodenal mucosa in gluten-fed broilers at 1 week (289 differentially expressed genes [DEGs]). Pathway analyses revealed that the significant DEGs were mainly involved in ribosome, oxidative phosphorylation, and peroxisome proliferator-activated receptor (PPAR) signaling pathways. These pathways are involved in ribosome protein biogenesis, oxidative phosphorylation and fatty acid metabolism, respectively. Our results suggest a pattern of differential gene expression in these pathways that can be linked to chronic inflammation, suppression of cell proliferation, cell cycle arrest and apoptosis. And via such a mode of action, high-gluten inclusion levels in poultry diets could lead to the observed retardation of villi development in the duodenal mucosa of young broiler chicken.

Association Between Working Hours and Depressive Symptoms Among Korean Employees

  • Kim, Eun Soo;Jeon, Sang Won;Kim, Mukyeong;Oh, Kang-Seob;Shin, Dong-Won;Park, Jae-Hyun;Cho, Sung Joon;Shin, Young-Chul
    • Korean Journal of Biological Psychiatry
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    • v.29 no.2
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    • pp.46-55
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    • 2022
  • Objectives Many studies have reported noticeable increases in the proportion of employees working either relatively short or relatively long hours. Such trends have been accompanied by an increasing concern that how much subjective mental well-being of employees would be influenced by their hours of work. The aim of this study was to investigate the association between work hours and clinically relevant depressive symptoms with demographic variables adjusted. Methods Participants were employees of a total of 56 private companies and local government organizations in Korea, aged 19 to 65 years. A self-report questionnaire that included items on working hour, job stress, levels of depression, and socio-demographic factors was administered to 15360 Korean employees, with 14477 valid responses. Hierarchical linear regression analyses, adjusted for sociodemographic factors, job related demographic factors, job stress, were used additionally to estimate the association between working hours and depressive scores. Results We found that working more than 40 hours per week correlated positively with the level of depressive symptoms after adjusting for demographic variables and the level of job stress. Furthermore, working 40 or fewer hours per week correlated negatively with the level of depressive symptoms. Being younger (β = -0.078, β = -0.099), being a female (β = 2.770, β = 1.268), and possessing a lower level of education (β = -0.315, β = -1.125) were significantly associated with higher level of depressive symptoms in all respondents. Conclusions Both of working excessively long or short hours is significantly associated with the prevalence of depressive symptoms. Establishing proper office hours for employees is critical to improving the quality of working conditions and maintaining good mental health in the workplace.

Influence of product category and features on fashion recommendation service algorithm (패션 추천서비스 알고리즘에서 상품유형과 속성 조합의 영향)

  • Choi, Ji Yoon;Lee, Kyu-Hye
    • Journal of the Korea Fashion and Costume Design Association
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    • v.24 no.2
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    • pp.59-72
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    • 2022
  • The online fashion market in the 21st century has shown rapid growth. Against this backdrop, using consumer activity data to provide customized customer services has emerged as a viable business model that draws attention. Algorithm-based personalized recommendation services are a good example. But their application in fashion products has clear limitations. It is not easy to identify consumers' perceptions of the attributes of fashion, which are various, hard to define, and very sensitive to trends. So there is a need to compile data on consumers' underlying awareness and to carry out defined research to increase the utilization of such services in the fashion industry and further engage consumers. This research aims to classify the attributes and types of fashion products and to identify consumers' perceptions of a given situation where a recommendation service is offered. To find out consumers' perceptions of and satisfaction with recommendation services, an online and mobile survey was conducted on women in their 20s and 30s, a group that uses recommendation services frequently. A total of 455 responses were used for analysis. SPSS 28.0 was used, combined with Conjoint Analysis and multiple regression, to analyze data. The study results could provide insights into a better understanding of recommendation services and be used as basic data for companies to identify consumers' preferences and draw up a detailed strategy for market segmentation.

Moderate dietary boron supplementation improved growth performance, crude protein digestibility and diarrhea index in weaner pigs regardless of the sanitary condition

  • Cho, Hyun Min;Macelline, Shemil Priyan;Wickramasuriya, Samiru Sudharaka;Shin, Taeg Kyun;Kim, Eunjoo;Son, Hong Cheol;Heo, Jung Min
    • Animal Bioscience
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    • v.35 no.3
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    • pp.434-443
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    • 2022
  • Objective: The study was conducted to investigate the impact of boron supplementation on nutrient digestibility, inflammatory responses, blood metabolites and diarrhea index, and their relevance to growth performance in weaned pigs housed in good and poor sanitary environments for 14 days after weaning. Methods: A total of 108 male pigs (Duroc×[Yorkshire×Landrace]) weaned at 21 days of age were used in a randomized complete block design with 2×3 factorial arrangement. Pigs were assigned to three boron treatments (0, 5, and 10 mg/kg) under two environments (good and poor sanitary) to give six replicates per treatment (3 pigs per replicate). On 0, 7, and 14 days, one pig per replicate was euthanized to collect, ileum tissue samples, and rectal fecal samples. Results: Boron supplementation quadratically influenced (p<0.001) feed intake and weight gain in pigs housed in good sanitary conditions from 1 to 14 days post-weaning where pigs offered 5 mg/kg boron optimized weight gain and feed intake. There is a quadratic interaction (p = 0.019) on feed intake for 1 to 14 days post-weaning where 5 mg/kg boron increased feed intake in good sanitary conditions. Pigs housed in the poor sanitary environment decreased (p<0.001) villus height and crypt depth in ileum at days 7 and 14. On day 7 and 14, crude protein digestibility was quadratically influenced (p<0.05) by boron supplementation. Boron supplementation linearly increased (p<0.05) plasma calcium and cholesterol levels whilst linearly (p = 0.005) reducing plasma triglyceride concentrations. Diarrhea index was quadratically influenced (p<0.05) by boron supplementations regardless of sanitary conditions where 5 mg/kg boron inclusion achieved the lowest diarrhea index. Conclusion: Pigs offered 5 mg/kg of boron increased weight gain which may be deduced by improved dry matter, crude protein, and energy digestibility regardless of the sanitary conditions.

Collapse-resistant performance of a single-story frame assembly and multi-story sub-frame under an internal column-removal scenario

  • Zhong, Wei-hui;Tan, Zheng;Tian, Li-min;Meng, Bao;Zheng, Yu-hui;Daun, Shi-chao
    • Steel and Composite Structures
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    • v.41 no.5
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    • pp.663-679
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    • 2021
  • To elucidate the differences in the collapse behavior between a single-story beam-column assembly and multi-story frame, two 1/3-scale two-bay composite frames, including a single-story composite beam-column assembly and a three-story composite sub-frame, were designed and quasi-statically tested. The load-displacement responses, failure modes, and internal force development of the two frames were analyzed and compared in detail. Furthermore, the resistance mechanisms of the two specimens were explored, and the respective contributions of different load-resisting mechanisms to the total resistances were quantitatively separated to gain deeper insights. The experimental tests indicated that Vierendeel action was present in the two-dimensional multi-story frames, which led to an uneven internal force distribution among the three stories. The collapse resistance of TSDWA-3S in the flexural stage was not significantly increased by the structural redundancy provided by the additional story, as compared to that of TSDWA-1S. Although the development of the load response was similar in the two specimens at flexural stage, the collapse mechanisms of the multi-story composite frame were much more complicated than those of the single-story beam-column assembly, and the combined action between stories was critical in determining the internal force redistribution and rebalancing of the remaining structure.

An Examination of Preconditions for the Creation of Collective Intelligence (집단지성 발현의 선행요인 검토)

  • Chu, Cheol Ho;Ryu, Suyoung
    • Knowledge Management Research
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    • v.23 no.3
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    • pp.213-229
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    • 2022
  • This study aimed to reveal factors that contribute to the creation of collective intelligence (CI) and to provide a cornerstone for future studies on this subject. We hypothesized that effects of the complexity and meaningfulness of the task, diversity, openness to experience, independence, decentralization, and the use of information and communication technology (ICT) are preconditions for the creation of CI. To investigate these hypotheses, we surveyed 200 individuals in the research and development-based manufacturing industry and collected a total of 185 valid responses. The results of the analysis showed that the meaningfulness of the task, openness to experience, independence, decentralization, and the use of ICT had positive effects on CI. Both perceived dissimilarity and value diversity had negative effects on CI. When all variables were included, their significance for the creation of CI showed the following order: use of ICT, the meaningfulness of the task, openness to experience, perceived dissimilarity, and value difference. The theoretical and empirical implications of these results were discussed.

Consumer Acceptance Intention of AI Fashion Chatbot Service -Focusing on Characteristics of Chatbot's Para-social Presence- (AI 기반 패션 챗봇 서비스에 대한 소비자 수용의도 -챗봇의 준사회적 실재감 특성을 중심으로-)

  • Hur, Hee Jin;Kim, Woo Bin
    • Journal of the Korean Society of Clothing and Textiles
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    • v.46 no.3
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    • pp.464-480
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    • 2022
  • With the steady development of Artificial Intelligence (AI), online stores are adopting chatbot services as virtual shopping assistants. This study proposes the concept of para-social presence to explore the undiscovered role of fashion chatbots' emotional and relational characteristics on service acceptance. Based on the Technology Acceptance Model (TAM), this study investigates the effect of a chatbot's para-social presence on service acceptance intention through consumers' beliefs. The web-based experiment was conducted on adult consumers who experienced chatbot services in an online shopping situation. A total of 247 responses were analyzed using confirmatory factor analysis, structural equation modeling, and multi-group SEM by AMOS 21.0 and SPSS 23.0. The findings illustrate that the chatbot's intimacy positively influenced consumers' perceived enjoyment, while the chatbot's understanding had a significant effect on perceived usefulness and ease of use. The chatbot's involvement had a positive effect on all consumer beliefs. Moreover, perceived ease of use had a positive influence on usefulness. A greater level of perceived usefulness and enjoyment positively heightened consumers' service acceptance intention. This study also verifies the moderating role of a need for human interaction. Consumers with a high need for human interaction have a relatively low tendency to perceive chatbot services as useful.

The Effect of Perceptions of CRM-Performing Companies and NGOs on Donation Attitudes and SNS WOM Intentions (CRM수행기업과 NGO에 대한 인식이 기부태도와 SNS구전의도에 미치는 영향)

  • Lee, Eun-Mi
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.6
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    • pp.341-346
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    • 2022
  • With the recent paradigm shift towards 'sustainable management', corporate social responsibility is recognized as an important factor for the continuous and long-term growth of a company. This study examines how the perception of CRM performing companies and NGOs affect attitude toward donation. In addition, it was empirically investigates how the perception of donation affects the SNS word of mouth intention. In this study, a total of 180 usable responses were obtained for analysis, and SPSS and AMOS 26.0 were used to verify the validity and hypothesis of the study. As a result of the study, it was found that validity and reliability were secured. As a result of the hypothesis testing, all the hypotheses were supported, but the effect of the perception of the company implementing CRM on the donation attitude was very weakly supported. This study suggests that raising a positive awareness of CRM-performing companies and NGOs collaborating with them is an effective strategy to increase donation attitudes and SNS word-of-mouth intentions for CRM.

A Study on the Counseling Experience of Counselors on Video Counseling with Digital Mask (디지털가면을 활용한 화상상담에 대한 상담자들의 상담 경험 연구)

  • Cho, Eunsuk;Jang, Eun-Hee;Oh, Yoon-Seok
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.6
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    • pp.67-77
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    • 2022
  • The purpose of this study was to explore the experiences of counselors who conducted video counseling using digital mask. To this end, the contents of the focus group interview with four counselors who experienced mask video counseling for a total of 10 Korean college student clients were analyzed using thematic analysis method. Participant counselors reported that they had been concerned about mask video counseling before the start but gradually be adjusted to the method. Since they have observed the rapid self-disclosure of the clients and smooth counseling process, they positively predicted the possibility of digital masks as a therapeutic media. They also mentioned the need for additional education and training for the counselors who are using the new on-line counseling media. Therefore, various supports for proactive responses of counselors to online counseling media need to be explored.

Evaluation of Durum Wheat Genotypes for Resistance against Root Rot Disease Caused by Moroccan Fusarium culmorum Isolates

  • Bouarda, Jamila;Bassi, Filippo M.;Wallwork, Hugh;Benchacho, Mohammed;Labhilili, Mustapha;Maafa, Ilyass;El Aissami, Aicha;Bentata, Fatiha
    • The Plant Pathology Journal
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    • v.38 no.1
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    • pp.1-11
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    • 2022
  • Fusarium culmorum is one of the most important causal agents of root rot of wheat. In this study, 10 F. culmorum isolates were collected from farms located in five agro-ecological regions of Morocco. These were used to challenge 20 durum wheat genotypes via artificial inoculation of plant roots under controlled conditions. The isolate virulence was determined by three traits (roots browning index, stem browning index, and severity of root rot). An alpha-lattice design with three replicates was used, and the resulting ANOVA revealed a significant (P < 0.01) effect of isolate (I), genotype (G), and G × I interaction. A total of four response types were observed (R, MR, MS, and S) revealing that different genes in both the pathogen and the host were activated in 53% of interactions. Most genotypes were susceptible to eight or more isolates, while the Moroccan cultivar Marouan was reported resistant to three isolates and moderately resistant to three others. Similarly, the Australian breeding line SSD1479-117 was reported resistant to two isolates and moderately resistant to four others. The ICARDA elites Icaverve, Berghisyr, Berghisyr2, Amina, and Icaverve2 were identified as moderately resistant. Principal component analysis based on the genotypes responses defined two major clusters and two sub-clusters for the 10 F. culmorum isolates. Isolate Fc9 collected in Khemis Zemamra was the most virulent while isolate Fc3 collected in Haj-Kaddour was the least virulent. This work provides initial results for the discovery of differential reactions between the durum lines and isolates and the identification of novel sources of resistance.