• 한국자원식물학회지
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• 제21권6호
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• pp.413-419
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• 2008

Phenolics level, total flavonoid content, and antioxidant were determined from the methanol extracts of the eight medicinal plants using roots. Total phenolics were found as the highest levels in the methanol extracts of Arctium lappa, and followed by Youngia sonchifolia and Cirsium japonicum. Total amount of the each phenol compounds were detected in C. japonicum extracts ($319.2\;mg\;kg^{-1}$) as the greatest component, and followed by A. lappa ($96.3\;mg\;kg^{-1}$) and Y. sonchifolia ($22.9\;mg\;kg^{-1}$). Total flavonoid content showed the highest amount in methanol extracts from A. lappa (68.1 mg $100\;g^{-1}$) and followed by Y. sonchifolia (11.2 mg $100\;g^{-1}$) Lycoris radiate extracts (87.2%) had the highest nitrite scavenging activity and followed by A. lappa (81.5%) and Y. sonchifolia (77.5%). Methanol extracts of A. lappa at 25 mg $100\;g^{-1}$ exhibited the highest DPPH radical scavenging activity by 90.8%, even though less activity than synthetic antioxidants Vitamin C or butylated hydroxytoluene (BHT). Level of polyphenols was highly correlated with anti oxidative activity ($r^2$ = 0.85). The results suggest that several medicinal plants selected had the potent biological activities, and that their activities were differential depending on plant species.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2021년도 춘계학술대회
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• pp.137-137
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• 2021

The study of total phenol (TP) content from 700 rice varieties was evaluated using the UV-Vis spectrophotometric method. The calibration curve of serial diluted gallic acid as a standard of this study showed the acceptable performances (R² = 0.999, mean accuracy 90%) and the mean of % relative standard deviation (%RSD: 0.07%) within the range of 7.8 to 1000 ppm concentrations. The mean value of total phenol content from 700 rice varieties was 2723.15 ㎍/g ranged from 55.48 ㎍/g to 9922.23 ㎍/g and the mean %RSD was 2.5%. Furthermore, this study aim was to analyze and profile individual phenolic compounds in the rice genetic resources to construct an integrative database for development of new rice variety with high functionality for health and understanding of phenolics characteristics in the rice grain. Herein, we analyzed selected 100 rice varieties based on high TP content and identified total 15 phenolic compounds by LC-ESI-MS/MS. Among selected 100 rice genetic resources, the phenolic metabolites consisted of higher amount of flavonoid (catechin) and phenolic acid mainly protocatechuic acid. Further research of more selected rice genetic resources would be continued to provide for an integrative phenolics profile of different rice genetic resources.

• 원예과학기술지
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• 제30권3호
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• pp.301-307
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• 2012

오디의 건강기능성 자원으로서의 가치를 높여 이용성을 증가하기 위해 '대성뽕', '수원뽕', '청일뽕'의 오디 3종과 함께 블루베리, 딸기를 시료로 사용하여 기능성 성분의 함량 및 항산화 활성을 측정하고 비교해 보았다. 시료 과실의 60% 에탄올 추출물의 총 phenol성 화합물, 총 flavonoid 함량 및 trans-resveratrol 함량은 '대성뽕'이 가장 높았고, 다음은 '수원뽕'이 높았으며, 총 anthocyanin 함량은 '대성뽕' 다음으로 블루베리가 높았다. 공시된 과실 추출물의 DPPH 및 ABTS free radical 소거능, FRAP에 의한 항산화 활성은 총 phenol성 화합물, 총 flavonoid, 총 anthocyanin 함량과 비례하여 증가하는 경향이었으며, 항산화 활성과 기능성 성분간에는 높은 상관관계가 있었다. 국내산 '대성뽕' 품종 오디는 국제적 건강기능성 과실인 블루베리보다도 기능성 성분과 항산화 활성이 현저하게 높아 이의 추출물은 기능성 식품소재로 이용하여 오디 소비 증진에 기여할 수 있을 것으로 생각되었다.

• 한국양봉학회지
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• 제32권4호
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• pp.327-332
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• 2017

In this study, we studied the changes in antioxidant activity of Actinidia arguta fruit of Autumn Sense cultivar during fruit ripening. The aim of this investigation was to find the knowledge of the changes of physiochemicals associated with fruit quality, antioxidant properties (free-radical scavenging activity and reducing power), total phenolics and vitamin C during fruit ripening. The highest free-radical scavenging activity (at $100{\mu}g/ml$) and reducing power (at $100{\mu}g/ml$) in A. arguta fruit were 78.57% and 0.22, respectively. Total phenolic content and vitamin C content in fruit of 10 days after fruit set were $639.48{\mu}g/g$ and $1052.2{\mu}g/g$, respectively. In general, the antioxidant activity and the related parameters, including total phenolic content and vitamin C content decreased during fruit ripening. These results improve knowledge of the effect of ripening on the antioxidant activity and related compounds contents that could help to establish the optimum A. arguta fruit harvest data for various usages.

• 원예과학기술지
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• 제30권6호
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• pp.769-775
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• 2012

Among the phenolic compounds that is generally present in strawberry fruits, five simple phenolics, three flavonoids, and a stilbene were tested for their antimicrobial activity against seven fungi and one oomycete. trans-Cinnamic acid showed strong antimicrobial activity, and the antimicrobial effect of the simple phenolics decreased with an increase in the number of hydroxyl groups. Phytophthora capsici was the most susceptible to the phenolic compounds tested in this study. trans-Cinnamic acid, p-hydroxybenzoic acid, and kaempferol were mainly detected in 'Seolhyang' strawberry fruits, and the total phenolic contents of the fruits decreased during their development. Extracts of the green (1-10% red color) and red (above 90% red color) strawberry fruits reduced the mycelial growth and zoospore germination rate of P. capsici, and the extract of red strawberry fruit showed strong antimicrobial activity against the zoospore germination of P. capsici. These results indicate that strawberry fruits contain antimicrobial phenolic compounds and that strawberry fruit extract can be used as a natural fungistat.

• Current Research on Agriculture and Life Sciences
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• 제28권
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• pp.53-62
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• 2010

원적외선 조사가 포도씨 기능성분의 변화에 미치는 영향을 알아보고자 가열출력(0, 900, 1800 W) 및 가열시간(0, 20, 40 min)을 달리하여 원적외선 조사 후 마이크로파를 이용하여 포도씨 추출물을 제조하고 기능성분으로서 가용성 고형분, 기계적 색도, 카테킨, 총 페놀, 총 플라보노이드 함량 및 전자공여능과 아질산염 소거능을 각각 측정하였다. 가용성 고형분 함량 및 백색도 및 적색도는 유의적인 차이가 없는 것으로 나타났다. Cathechin류는 가열시간에 따라 catechin, procyanidin $B_2$, epicatechin 함량의 변화가 있음을 확인하였으며, 총 catechin 함량은 총 카테킨 함량은 900 W 및 20분 처리 시 가장 높게 나타났다. 총 페놀성화합물 및 플라보노이드 함량 또한 가열출력 900W 및 가열시간 20분 일 때 가장 높게 나타났다. 그에 따라 전자공여능도 가열출력 900 W 및 가열시간 20분 일 때 가장 높은 것으로 나타났다. 이상의 결과는 원적외선 처리에 의해 총 페놀성 화합물 함량, 총 플라보노이드 함량이 증가됨을 볼 수 있었으며, 특히 cathechin류의 변화가 있음을 확인할 수 있었다. 또한 이로 인해 항산화능도 증가됨을 알 수 있었다. 또한 포도씨의 원적외선 가열처리 최적조건을 설정하고자 가열출력 및 가열시간을 달리하여 중심합성실험계획으로 반응표면분석한 결과 가열출력 621.82~818.18 W, 가열시간 16.3~19.83 min의 범위가 최적 가열조건범위로 설정되었다.

• 동아시아식생활학회지
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• 제18권4호
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• pp.499-506
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• 2008

The nutritional composition of the white lotus(Nelumbo nucifera Gaertn) leaf are moisture($1.87{\pm}0.06%$), crude ash($9.57{\pm}0.07%$), crude protein($15.53{\pm}0.4%$) and crude lipid($2.42{\pm}0.1%$) per 100g. The highest mineral content was K and Ca. The principal free sugars in the white lotus leaf was sucrose, fructose and glucose. The total phenolics compounds evidenced maximal levels of $8,842.73{\pm}20.57\;g/100g$. DPPH and hydroxyl radical of scavenging ability of each sample tended to increase with increasing degrees of the sample concentration. The $IC_{50}$ values of the ethanol extract, n-hexane, chloroform, ethylacetate, butanol and water fraction from the white lotus leaf to DPPH radicals were 5.65, 8.5, 8.27, 2.03, 2.3 and $13.7\;{\mu}g/mL$ respectively. The $IC_{50}$ values of the hydroxyl radical scavenging ability of the ethanol extract, ethylacetate, butanol and water fraction were 390, 574, 327 and $378\;{\mu}g/mL$ respectively.

• 한국식품영양과학회지
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• 제32권3호
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• pp.338-345
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• 2003

복분자 추출에 따른 페놀성화합물의 특성변화를 조사하기 위하여 반응표면분석법으로 시료에 대한 용매비(5, 10, 15 mL/g), 에탄을 농도(20, 40, 60%) 및 추출시간(2, 4, 6 hr)을 달리하여 가용성 고형분 함량, 총 페놀성 화합물 함량, 전자 공여능, 항산화능 및 아질산염소거능의 변화를 모니터링 하였다. 가용성 고형분 함량, 총 페놀성 화합물 함량, 전자공여능 및 항산화능은 시료에 대한 용매비의 영향을 많이 받는 것으로 나타났다. 아질산염 소거능의 경우 pH 1.2 및 6.0에서는 에탄을 농도의 영향을 많이 받았으며, pH 3.0및 4.2에서는 추출시 간의 영 향을 많이 받는 것으로 나타났다. 가용성 고형분 함량에 있어서 최적 추출조건은 시료에 대한 용매비 14.16mL/g, 에탄을 농도 39.08% 및 추출시간 2.99 hr으로 나타났으며, 총페놀성 화합물 함량의 경우 시료에 대한 용매비 14.06mL/g, 에탄을 농도 35.51% 및 추출시간 3.15 hr으로 나타났다

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.297-303
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• 2020

Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.

• 한국식품과학회지
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• 제49권6호
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• pp.714-716
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• 2017

장미꽃을 $30^{\circ}C$, 상대습도 60%의 조건에서 24시간 동안 숙성하여 총페놀화합물 함량, 산화방지 활성, 카테킨 함량을 최대로 증가시키는 조건을 수립하였다. 장미꽃을 차로 이용하기 전에 숙성공정을 통하여 생리활성 물질인 카테킨을 비롯한 페놀화합물의 추출 효율을 증가시킬 수 있고 이에 따라 장미꽃 차의 산화방지효과를 증대 시킬 수 있다.