• Title/Summary/Keyword: total free amino acid contents

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Evaluation in physicochemical properties of soy sauce fortified with soymilk residue (okara koji) (비지 koji 첨가에 따른 양조간장의 발효 중 이화학적 특성 평가)

  • Song, Young-Cheol;Lee, Sam-Pin
    • Food Science and Preservation
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    • v.20 no.6
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    • pp.818-826
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    • 2013
  • To evaluate the practical use of okara koji in soy sauce fermented with soybean koji, the okara koji was fortified with different contents (0, 50, and 100%) in soybean koji and then fermented for 90 days. The saltiness of the soy sauce was about 17.15~17.22%. The higher okara koji content showed lower net soluble solid contents of 8.73, 6.12, and 2.50%, as well as lower acidity levels of 1.09, 0.98, and 0.47%. The buffering capacity of the soy sauce decreased to 1.26-3.41 by adding higher okara koji. The protease activity was higher in the soy sauce with 50% orara koji and decreased with longer fermentation. Also, the tyrosine content peaked to 275.2 mg% after 90 days. ${\alpha}$-amylase showed higher activity in the soy sauces fortified with okara koji, which resulted in the highest total sugar and reducing sugar contents after 60 days. The total sugar and the reducing sugar in the soy source decreased after longer fermentation. The total free amino acid contents of the soy sources fortified with okara koji (0, 50, and 100%) were 41.68 mg/mL, 33.10 mg/mL, and 9.27 mg/mL, respectively. In particular, the glutamic acid contents of the three types of soy sauces were highest, and most amino acids, except for glutamine, increased during the fermentation for 90 days. The sensory evaluation, except of the saltiness and color, showed similar values in the soy sauces, except in the okara koji 100%. Thus, okara koji could be a valuable ingredient of traditional soy sauces. However, the 50% okara koji did not differ significantly from the others.

Quality of Insambob Containing Added Raw and Red Ginseng Extract (수삼과 홍삼액을 첨가하여 취반한 인삼밥의 품질학적 특성)

  • Lee, Ka-Soon;Kim, Gwan-Hou;Kim, Hyun-Ho;Seong, Bong-Jae;Kim, Sun-Ick;Han, Seung-Ho;Lee, Gyu-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.8
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    • pp.1151-1157
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    • 2012
  • This study was conducted to investigate methods of increasing raw ginseng consumption. To accomplish this, Insambob was prepared by adding minced raw ginseng (MRG), ground raw ginseng (GRG) or extracts of red ginseng (RGE). Sensory quality, textural properties, and changes in the ginsenoside and free amino acid composition of the Insambob then were investigated. Insambob containg 50% RGE had the best color, flavor and texture, but that containing 10% GRG had the best taste and overall acceptability. The hardness and adhesiveness were highest for containing 10% GRG and decreased as the amount of ginseng added increased. However, the hardness increased, while the adhesiveness of Insambob containg RGE decreased significantly as the amount added increased. Moreover, the ginsenoside composition changed upon addition of ginseng, with the levels of ginsenoside-Rb1, -Rb2, -Rb3, -Rc, -Re, -Rd, -Rg1, and -Rf decreasing and ginsenoside-Rh2, -Rh1, and -Rg3 newly appearing. Finally, the total free amino acid contents of Insambob increased upon addition of MRG, GRG and RGE.

Effects of Ice Cooling Storage on Chemical Components in Vegetable Corn (풋옥수수의 얼음 저장이 종실성분 변화에 미치는 영향)

  • 손영구;김성열;김선림;황종진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.1
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    • pp.95-103
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    • 1997
  • This experiment was carried out to obtain the basic information necessary to establish suitable postharvest handling techniques and to keep high quality of the sweet(Danok 2), supersweet(Cooktail 86) and waxy(Chalok 1) corn which are mainly consumed as vegetable in Korea. Vegetable corns were cooled with ice fragments in the insulation box immediately after harvest and stored in low temperature warehouse at 0 to 2$^{\circ}C$. During the 15 days short-term storage, changes of chemical components were compared with those of uncooled corns. The losses of moisture in kernels were as high as 7.4 to 24.4% in uncooled corns while those of ice cooled corns increased 0.4 to 0.5% of their weight. The ratio of pericarp and alcohol insoluble solid(AIS) content increased as the storage days prolonged in all treatments but increasing rates were much higher in uncooled samples. On the other hand, the total sugar loss during storage was the least in supersweet corn when they were cooled with ice fragments in insulation box. After 5 days storage, the ice cooled samples showed the highest free amino acid contents compare to those of uncooled and stored at room temperature (25 to 3$0^{\circ}C$) or low temperature warehouse, and ${\gamma}$-aminobutylic acid (GABA) which was known as a fuctional amino acid was detected in all three kinds of vegetable corns.

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Characterization of Fermented Mulberry Leaf Using Bacillus subtilis (Bacillus subtilis를 이용한 뽕잎 발효 특성)

  • Seo, Seung-Ho;Park, Seong-Eun;Kim, Eun-Ju;Oh, Dohgun;Son, Hong-Seok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.1
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    • pp.108-114
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    • 2017
  • This study was performed to evaluate the characterization of fermented mulberry leaf using Bacillus subtilis by focusing on protein degradation and antioxidant activity. The crude protein and total amino acid compositions of mulberry leaf were 21.40% (w/w) and 105.06 mg/g, respectively. The pH level decreased sharply in mulberry leaf extracts fermented using B. subtilis, in accordance with an increase in bacteria cell populations (9.49 log CFU/mL) during 36 h of fermentation. The protease activity of mulberry leaf increased to 97.45 units/mL after 5 days of fermentation. After fermentation, free amino acid contents in fermented mulberry leaf increased from $486.91{\mu}g/g$ to $644.35{\mu}g/g$ due to considerable elevation of isoleucine (6-fold), alanine (4.67-fold), leucine (4.52-fold), and valine (4.21-fold). The DPPH radical scavenging activity of fermented mulberry leaf also increased from 25.93% to 73.22% after 5 days of fermentation. These results suggest that mulberry leaf fermentation using B. subtilis can improve nutritional quality and antioxidant activity.

Food Components and Sensual Characterization of Commercial Plain-dried Silver-stripe Round Herring (시판 소건 샛줄멸의 식품성분 및 관능 특성)

  • Kim, Hyung-Jun;Yoon, Min-Seok;Park, Yong-Seok;Ha, Jin-Hwan;Jung, In-Kwon;Heu, Min-Soo;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.11
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    • pp.1457-1464
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    • 2008
  • For the effective use of under-utilized resources, silver-stripe round herring (SSRH), the plain-dried SSRH was investigated and compared with boiled-dried SSRH and boiled-dried anchovy on the food component and sensual characterization. Moisture content and salinity of the plain-dried SSRH were 31.9% and 7.6%, respectively. Moisture content of the plain-dried SSRH was higher, while the salinity was lower than those of boiled-dried SSRH and anchovy. According to the results of peroxide value, fatty acid composition, Hunter b value, and browning index, the rancidity was higher in plain-dried SSRH than in boiled-dried SSRH and anchovy. When compared to the boiled-dried SSRH and anchovy, the plain-dried SSRH was higher in calcium and phosphorus contents, while lower in total content of amino acid. According to the results of hot-water soluble-N, trichloroacetic acid-N, free amino acid, and taste value, the taste of plain-dried SSRH was superior to those of the boiled-dried SSRH and anchovy. The results of chemical components and sensory evaluation indicated that the plained-dried SSRH could be substituted for boiled-dried anchovy classified into special grade, if fish odor of SSRH can be improved.

Nutrients and Antioxidative Activities of Metasequoia glyptostroboides (메타세콰이어나무 열매와 잎의 영양성분 및 항산화 활성)

  • Bae, Young-Il;Lee, Ju-Won;Ha, Tae Jung;Hwang, Seung-Ha;Shin, Chang-Sik;Jeong, Chang-Ho;Kim, Il-Hun;Shim, Ki-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.3
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    • pp.363-368
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    • 2013
  • The nutrients and antioxidative activities of fruits and leaves from Metasequoia glyptostroboides were investigated to provide basic data for the future development of functional foods. The nitrogen-free extract contents of fruit and leaves were 69.04% and 50.47%, respectively. Total phenolic content was higher in leaves (21.75 mg/GAE g) compared to the fruit (19.95 mg/GAE g). The mineral components of fruit and leaves mainly consisted of calcium (2,136.08 and 304.85 mg/100 g, respectively), potassium (1,355.53 and 1,144.04 mg/100 g, respectively), and phosphorous (426.30 and 350.50 mg/100 g, respectively). In terms of amino acid composition, aspartic acid, glutamic acid, proline, and leucine were relatively high, but methionine and cystine were low. The hot water extract from leaves was a more potent free radical-scavenger and had higher reducing activities than extracts from fruit. Thus, phenolics of M. glyptostroboides leaves can be utilized as an effective functional food substance for its natural antioxidant properties.

Antioxidant capacity and nutritive components from biji sub-micron suspension by ultra-high pressure homogenization process (초고압균질 가공에 의해 제조된 초미세 비지 현탁액의 영양성분 및 항산화성)

  • Lee, Seon-Ho
    • Food Science and Preservation
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    • v.22 no.5
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    • pp.714-720
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    • 2015
  • Biji is a tofu by-product made by squeezing the liquid out of the dregs left over from making tofu. The objectives of this research were to break the biji into sub-micron-scale particles and to prepare a biji sub-micron suspension having increased quality characteristics such as antioxidant capacity, polyphenol, dietary fiber, and soluble protein contents, in comparison with untreated biji. Disruption of biji by an ultra-high-pressure homogenization (UHPH) process was used to prepare the sub-micron suspension of biji. UHPH can be used to prepare emulsions or suspensions with extremely small particle sizes. The effect of the UHPH process on biji was studied at 1,000, 1,500, and 2,000 bar. The extraction yield increased up to 1.64 times by breaking the biji to form a sub-micron suspension. The soluble dietary fiber, protein, and free amino acid contents were increased by the UHPH process (p<0.05), in comparison with those of untreated biji. Furthermore, the total sugar, free sugar, and total polyphenol contents of biji were augmented by the UHPH process. Antioxidant activity (ABTS) after exposure to UHPH (1,000-2,000 bar) was well retained (p<0.05). The extraction efficiencies and nutritive components were enhanced by increasing the pressure in the UHPH process.

Biological Activities and Physiochemical Properties of Gangwon-do Endemic Makjang Products (강원도 시판 막장제품의 이화학적 품질특성 및 생리활성 조사)

  • Kim, Byoung-Mok;Jung, Jee-Hee;Lim, Ji-Hoon;Jung, Min-Jeong;Jeong, Jae-Whung;Choi, Yong-Suck;Sim, Jea-Man;Jeong, In-Hak;Kim, Young-Myoung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.6
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    • pp.862-873
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    • 2015
  • In this study, we investigated the physiochemical properties and biological activities of Gangwon-do endemic Makjang (MJ) products (12 types). The pH levels of all samples were in the range of 4.43 to 5.69, and MJ5 showed the highest pH (5.69). The salinities of all samples ranged from 11.1% to 16.9%. Hunter color values for L (lightness), a (redness), and b (yellowness) ranged from 26.2 to 36.9, 3.9 to 11.5, and 6.5 to 16.6, respectively. The amino nitrogen content of MJ2 was highest, whereas the total content of free amino acids of MJ11 (4,657.7 mg%) was highest. Total fatty acid contents of all samples ranged from 1,598.6 mg% to 2,874.4 mg%, with MJ10 showing the highest fatty acid content. The content of total polyphenolic compounds ranged from 401.48 to $746.67{\mu}g$ tannic acid equivalent/mL, with MJ11 showing the highest content. The 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) radical scavenging effects of MJ11, MJ8, and MJ4 were 51.30% and 82.5%, 41.29% and 67.0%, and 49.88% and 87.7%, respectively. MJ12 showed the strongest growth inhibitory effect on lung cancer A549 cells, whereas MJ5 showed the strongest growth inhibitory effect on AGS gastric cancer cell and MCF-7 breast cancer cell. MJ7 showed greater lipid accumulation inhibitory activity in HepG2 cells than the others. ACE inhibitory activity of MJ11 was the highest among the samples.

Effects of Harvest Stage on Agronomic Characteristics, Yield and Feed Value of Silage Corn in the Newly Reclaimed Hilly Land (산지 신개간 토양에서 사료용 옥수수 수확시기가 생육특성, 생산성 및 사료가치에 미치는 영향)

  • Do, Gu-Ho;Kim, Eun-Joong;Lee, Sang-Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.3
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    • pp.253-264
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    • 2012
  • This study was carried out to investigate growth characteristics, yield, chemical compositions and nutrients yield of corn hybrids for silage in the newly reclaimed hilly land. The experimental design was arranged in a randomized block design with three replications. The seeding time was at May 6. The harvest time of four treatments was milk stage (97 days), dough stage (105 days), yellow stage (112 days) and late yellow stage (119 days after seeding). Plant height, ear height, leaf numbers and ear length were highest in yellow stage (p<0.05, 0.01), but dead leaf, stem hardness and sugar degree (Brix) were higher in late yellow than other treatments. Leaf width, tip filling degree and fresh yield were not significantly different. Dry matter yield increased as the maturity stage progressed (p<0.01). Crude protein and crude fat were not significantly different. NDF and ADF decreased as the maturity stage progressed (p<0.01). Ca content was the highest at milk stage (p<0.05), Fe and P were the highest at dough stage (p<0.05, 0.01). However another minerals were not significantly different. Essential amino acid (EAA), nonessential amino acid (NEAA) and total amino acid were highest at yellow stage, but no significant differences were found among the treatments. Total free sugar contents were higher in the order of Milk > dough > yellow > late yellow stage, but no significant differences were found among the treatments. Crude protein yield was the highest at yellow stage, but crude fat yield, amino acid yield and TDN yield were highest at late yellow stage (p<0.01). Total mineral yield showed no significant difference. Based on the above results, yellow and late yellow stage compared to other maturity stage have been shown to increase dry matter yield and nutrients yield, when silage corn grow cultivate in the newly reclaimed hilly land.

Changes of Chemical Components during Fermentation of Pear Wine (배술 발효 과정 중 화학 성분의 변화)

  • Lee, Ka-Soon;Park, Hae-Min;Hong, Jong-Sook;Lee, Gyu-Hee;Oh, Man-Jin
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.991-998
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    • 2009
  • We used pears to manufacture wine, and analyzed changes in pH, acidity and ethanol and sugar content during fermentation. Pear wine with added ginger (to improve quality) did not differ from ginger-free wine in pH or acidity level. The ethanol content of pear wine was the highest (13.0%, v/v) inpear wine with 0.1% (w/v) added ginger compared to pear wine with no ginger, and sensory tests examining taste and color yielded the highest scores for pear wine with 0.2% (w/v) ginger. To assess storage stability, pear wine was treated for 30 minutes at $55^{\circ}C$, $60^{\circ}C$, $65^{\circ}C$, or $70^{\circ}C$. Unheated pear wine showed rapid changes in pH and acidity level after 30 days of storage, whereas pear wine treated for 30 minutes at $60^{\circ}C$ did not show such changes. Total organic acid levels in pear wine increased by 0.71% and 0.89% (v/v), respectively. The free sugar level in pear wine decreased from 12.05% to 3.13% (w/v). Turning to phenolic compounds, caffeic acid, catechin, and epicatechin contents in pears were 1.64, 1.40, and 0.23 mg/100mL, respectively, with diverse compositions. Caffeic acid levels in pear wine decreased sharply to 0.12 mg/100 mL upon fermentation, whereas free catechin inpear wine increased to 1.16 mg/100 mL compared with 0.28 mg/100 mL in pears. Free arbutin increased from 8.34 mg/100 mL in pears to 10.39 mg/100 mL in pear wine. The free amino acid content of pear wine was 118.5 g/100 mL, but the levels of serine, alanine, glutamic acid, and aspartic acid decreased sharply upon fermentation, with corresponding increases in tyrosine, GABA, lysine, and arginine.