• 정보보호학회논문지
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• 제25권2호
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• pp.331-342
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• 2015

본 논문에서는 기초자치단체 277 기관 중 서울(25개 지역), 전북(14개 지역), 부산(16개 지역)을 선정하여 조례현황 및 관련 법률을 검토하였다. 자치법규 정보시스템을 활용하여 각 지역별 자치법규에서 운용되고 있는 조례 내의 별지서식 내용을 조사 분석하였다. 개인정보 공개 거부감이 있는 부분을 참고하여 총 4가지 항목(주민등록번호, 핸드폰번호, 자택전화번호, 소득정보)을 조사 분석 하였다. 이를 통해, 순환보직이 잦은 공공기관 담당자와 민간 컨설팅 담당자들이 법령과 각종 조례, 규칙을 이해하고 검토할 수 있도록 지자체 법규 개선 방안을 제안한다. 또한, 개인정보보호 관리체계를 활용하여 지자체 서식 개정 검토를 쉽게 할 수 있도록 문서 서식 규정과 별지 서식 검증 할 수 있도록 통제 항목추가를 제안하였다.

• 한국한의학연구원논문집
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• 제8권2호통권9호
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• pp.95-107
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• 2002

Yukmijihwang-tang(YM) is a noted herbal prescription in Chinese and Korean traditional medicines, and it has been known to reinforce the vital essence and has been widely used for a variety of disease such as stroke, osteoporosis, anti-tumor, and hypothyrodism. Regarding its traditional use, YM has been known to reinforce the Yin (vital essence) of liver and kidney. Also it has been known to reinforce nutrition and biological function in brain. Recently, studies suggested that YM increase antioxidant activities and exert the protective effect against oxidant-induced liver cell injury. We investigated the high-throughput gene expression analysis on the Yukmijihwang-tang administrated in SD rats. Microarray data were validated on a limited number of genes by semiquantitative RT-PCR and Western blot analyses. The recent availability of microarrays provides an attractive strategy for elaborating an unbiased molecular profile of large number of genes in drug discovery This experimental approach offers the potential to identify molecules or cellular pathways not previously associated with herbal medicine. Total RNA from normal control brain and Yukmijihwang-tang administrated brain were hybridized to microarrays containing 10,000 rat genes. The 52 genes were found to be up-regulated(twice or more) excluding EST gene. The nine genes were found to be down-regulated(twice or more) excluding EST gene. Gene array technology was used to identify for the first time many genes expression pathway analysis that arecell cycle pathway, apoptosis pathway, electron transport chain pathway, cytoplasmic ribosomal protein pathway, fatty acid degradation pathway, and TGF-beta signaling pathway. These differentially expressed genes pathway analysis have not previously been iavestigated in the context of herbal medicine efficacy and represent novel factors for further study of the mechanism of herbal medicine efficacy.

• 한국식품조리과학회지
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• 제16권6호
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• pp.557-567
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• 2000

An optional ingredient, jasoja(Perillae semen), was adopted to improve the quality of Dongchimi. The final weight percentage of jasoja in Dongchimi was adjusted to 0, 0.25, 0.5, 0.75, or 1.0%, per radish, and sensory and microbiological characteristics were determined during fermentation at 10 for 45 days. The effect was varied depending on the amounts of jasoja, but Dongchimi fermented with 0.5% jasoja was most favored for color, flavor, taste, texture, and overall acceptability in sensory evaluation. According to a quantitative descriptive analysis for the product, the liquid portion of Dongchimi steadily became clearer and less sour in proportion to the amount of added jasoja. However, a strong off-taste was detected from 1.0% treatment. The viable cell numbers of total and lactic acid bacteria drastically increased during the first 2 days, and then gradually increased to their maximum values during fermentation and slowly decreased at the later stage. Dongchimi with 0.5% treatment showed a distinctive high number of microorganisms at the 15th-day of fermentation and this trend was maintained until the completion of fermentation. The lactic acid bacteria isolated and identified from Dongchimi were; Lactobacillus brevis, Lactobacillus plantarum, Leuconostoc mesenteroides, Lactococcus faecalis, and Lactococcus lactis. The combined number of Lactobacillus brevis and Lactobacillus plantarum began to increase right after preparation to as much as 10$\^$7/CFU/㎖, then decreased to 10-10$^3$CFU/㎖ afterward. This study showed that the addition of jasoja retarded the initial fermentation of Dongchimi; however, too much jasoja at above 1% weight level per Chinese radish might accelerate fermentation at the later fermentation stage and shoud be avoided. A comparable fermentation pattern was observed among the samples; however, more acceptable Dongchimi could be prepared by fermenting for 11 to 30 days at 0.5% jasoja concentration per radish.

• 한국정보통신학회논문지
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• 제9권6호
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• pp.1387-1394
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• 2005

본 논문에서는 다중 셀룰러 CDMA 환경에서 비균일 트래픽 특성을 가진 지능정보 처리를 위한 호 수락제어 기법을 제안하고, 이 기법의 성능을 분석한다. CDMA 시스템에서는 각 셀에서 동시에 통화할 수 있는 이동 가입자들의 수는 각 기지국이 수신하는 총 간섭량에 따라 제약을 받고, 또한 서비스 받는 이동 가입자의 수는 각 기지국마다 균일하지 않게 분포될 수 있다. 본 논문에서는 이러한 환경에서 비균일 트래픽 특성의 지능정보 처리용량을 높이기 위해 등가대역 개념을 이용한 호 수락 제어 기법을 사용하며, 신규 호의 차단율(blocking rate) 및 핸드오프 호의 절단율(dropping rate)을 감소시키기 위해 각 셀의 대역폭을 가변시켜 신규 호와 핸드오프 호를 처리한다. 실험결과 다중 셀룰러 CDMA 환경에 제안한 호 제어 기법을 적용함으로써 이동 가입자의 수용 용량을 증가시킬 수 있음을 확인하였다.

• 한국식품위생안전성학회지
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• 제15권1호
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• pp.25-29
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• 2000

김치 공장에서 배추 절임시 사용하는 염수의 재사용 가능성을 확인하기 위하여 염지 후 배출되는 염수의 염농도를 재조정하여 6회까지 재 사용하면서 염수의 성분과 미생물 변화 및 매회 절임 배추로 담근 김치의 품질을 평가하였다. 배추 절임 후 염수의 염 농도는 1.35~2.49%감소하였고 재 사용횟수에 따라 절임 후 염수의 pH는 4회까지 유의적 변화를 보였고 절임 전 염수의 pH는 4회 까지는 통계적 유의차가 없었으며 산도도 절임 횟수에 따라 증가하는 경향을 보였다. 염수 중 미생물수는 1회 사용시 1.58$\times$10에서 4회 사용시 2.25$\times$$10^{7}$ 으로 최고에 달하여 10배정도 상승하였다. 염수 중 염을 제외한 순 가용성 고형분은 6회 사용시 0.93%로 미미하게 증가하는 경향이었고 비타민 C함량은 6회사용 후 0.55 mg%로 재사용 횟수에 따라 상승하는 경향을 보였다. 6회까지 염수를 재 사용하여 절임한 배추로 담근 김치의 pH와 산도, 그리고 관능적 품질은 유의적 차이를 보이지 않아 염수의 재 사용가능성을 확인하였다.

• 한국식품과학회지
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• 제27권5호
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• pp.813-818
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• 1995

부추추출물의 김치산패 억제효과를 검토하기 위하여 부추 MeOH추출물을 동결건조하여 powder형태로 첨가시기를 달리하여 김치에 첨가하고 pH 및 산도의 변화, 총균수, Lactobacillus속, Leuconostoc속, Pediococcus속 및 Streptococcus속 그리고 효모수의 변화를 측정하였다. pH의 변화는 김치 담금초기에 부추추출물을 첨가한 김치가 pH 감소가 작았고 그 다음으로 숙성 1일째 첨가한 김치에서 차이가 보였지만 숙성 2일${\sim}$4일째 첨가한 김치는 pH 변화가 대조구 김치와 같은 양상을 보였다. 산도의 변화는 김치 담금 초기에 첨가한 김치에 비해 산도 증가 속도가 낮았으며 그 다음으로 숙성 1일째 첨가한 김치는 산도증가가 낮았으나 숙성 2일${\sim}$4일째 첨가한 김치의 산도의 변화는 대조구 김치의 산도변화와 비슷한 경향을 나타냈다. 김치발효 관련 미생물의 변화는 김치 담금초기에 부추추출물을 첨가한 김치에서 생육 억제효과가 인정되었고 특히 Pediococcus속에 대해서는 뚜렷하였으며 Lactobacillus속 및 Leuconostoc속에 대해서도 차이를 보였다. 그 다음으로 숙성 1일째에 첨가한 김치에서 생육 억제효과가 나타났다. 이러한 결과는 pH, 산도 변화에서 김치 담금초기에 부추추출물을 첨가한 김치 및 숙성 1일째 첨가한 김치의 결과와 일치하였다. 부추추출물의 첨가시기는 김치담금초기가 가장 효과적이며 그 다음으로 숙성 1일째가 효과적이며 부추추출물을 첨가한 김치의 산패 지연효과는 1.5일로 나타났다.

• 한국동물위생학회지
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• 제30권3호
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• pp.467-472
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• 2007

Eurasian black vulture (Aegypius monachus) belongs to family Accipitridae and is a raptor preying small birds and mammals. It is a wintering bird residing from the mid of November to the late of March in Korea. Although Eurasian black vulture population wintering at Korea is gradually increased, the efficient rescue and treatment for injured Eurasian black vultures are still problematic, due to lack of basic data. Therefore, in this survey, to obtain basic resources for establishing more efficient rescue system, we surveyed the number of Eurasian black vultures wintering at Gangwon province, wintering ecology and injury state of Eurasian black vulture. Survey area was Togyo reservoir at Cheorwon, Seonan basin at Yangoo and Wolsong reservoir at Chuncheon from November 2004 to March 2005. From 12 rescued Eurasian black vultures, physical examination, total plasma protein (TPP) and packed cell volume (PCV) were measured. To survey injury state of Eurasian black vultures wintering at Gangwon province, we surveyed the cause, morbidity and mortality from 103 Eurasian black vultures, based on medical records from animal rescue teams. The number of eurasian black vultures were gradually increased from November, peaked at Feburary, then steeply decreased at March. Eurasian black vultures showed the most vigorous activity around 1 PM within a day. In physical examination, mean body weight was $9.6{\pm}1.22kg$, body length was $1130{\pm}45.4mm$. No statistically significant difference in body weight and length between male and female eurasian black vultures was observed. Mean PCV was $40.2{\pm}2.7%$, while mean TPP was $5.86{\pm}0.8mg/dl$. The most common cause of injury and mortality was intoxication, while that of morbidity was fatigue.

• 한국수정란이식학회지
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• 제31권1호
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• pp.39-46
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• 2016

Cryopreservation has been applied successfully in many mammalian species. Nevertheless, pig embryos, because of their greater susceptibility to cryoinjuries, have shown a reduced developmental competence. The aim of this study was to evaluate the survival status of vitrified-warmed porcine embryos. Forced blastocoele collapse (FBC) and non-FBC blastocysts are vitrified and concomitantly cultured in culture media which were supplemented with/without fetal bovine serum (FBS). Porcine vitrified-warmed embryos were examined in four different methods: group A, non-FBC without FBS; group B, non-FBC with FBS; group C, FBC without FBS; group D, FBC with FBS. After culture, differences in survival rates of blastocysts derived from vitrified-warmed porcine embryos were found in group A~D (39.5 (A) vs 52.5 (B) and 54.8 (C) vs 66.7% (D), respectively, p<0.05). Reactive oxygen species (ROS) level of survived blastocysts was lower in group D than that of another groups (p<0.05). Moreover, total cell number of survived blastocysts was higher in group D than that of other groups (p<0.05). Otherwise, group D showed significantly lower number of apoptotic cells than other groups ($2.0{\pm}1.5$ vs $3.2{\pm}2.1$, $2.8{\pm}1.9$, and $2.7{\pm}1.6$, respectively, p<0.05). Taken together, these results showed that FBS/FBC improves the developmental competence of vitrified porcine embryos by modulating intracellular levels of ROS and the apoptotic index during the vitrification/warming procedure. Therefore, we suggest that FBS and FBC are effective treatment techniques during the vitrification/warming procedures of porcine blastocysts.

• Osong Public Health and Research Perspectives
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• 제8권6호
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• pp.389-396
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• 2017

Objectives: To circumvent the limitations of the current golden standard method, colony-forming unit (CFU) assay, for viability of Bacille Calmette-$Gu{\acute{e}}rin$ (BCG) vaccines, we developed a new method to rapidly and accurately determine the potency of BCG vaccines. Methods: Based on flow cytometry (FACS) and fluorescein diacetate (FDA) as the most appropriate fluorescent staining reagent, 17 lots of BCG vaccines for percutaneous administration and 5 lots of BCG vaccines for intradermal administration were analyzed in this study. The percentage of viable cells measured by flow cytometry along with the total number of organisms in BCG vaccines, as determined on a cell counter, was used to quantify the number of viable cells. Results: Pearson correlation coefficients of FACS and CFU assays for percutaneous and intradermal BCG vaccines were 0.6962 and 0.7428, respectively, indicating a high correlation. The coefficient of variation value of the FACS assay was less than 7%, which was 11 times lower than that of the CFU assay. Conclusion: This study contributes to the evaluation of new potency test method for FACS-based determination of viable cells in BCG vaccines. Accordingly, quality control of BCG vaccines can be significantly improved.

• Journal of Animal Science and Technology
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• 제64권3호
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• pp.432-442
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• 2022

The purpose of this study was to analyse the effects of light colour on rabbit reproductive performance and the expression of key follicular development genes. Rabbits (n = 1,068, 5 months old, 3.6-4.4 kg live body weight) were divided randomly into four groups, housed individually in wire mesh cages and exposed to red, green, blue, and white light-emitting diode (LED) light (control). The lighting schedule was 16 L : 8 D-15 d / 150 lx / 6:00 am-22:00 pm (3 d preartificial insemination to 12 d postartificial insemination). Red light and white light affected the conception rate and kindling rate and increased the total litter size at birth (p < 0.05). The effects of red light on litter size at weaning, litter weight at weaning, and individual weight at weaning increased compared with the green and blue groups. The effects of red light on live litter size at birth were increased compared with those in the blue group (p < 0.05). Compared to white light, green and blue light reduced the number of secondary follicles (p < 0.05). Compared to red light, green and blue light reduced the number of tertiary follicles (p < 0.05). Compared with white light, red LED light resulted in greater ovarian follicle stimulating hormone receptor and luteinizing hormone receptor mRNA expression (p < 0.05). Compared with green and blue LED light, red LED light resulted in greater B-cell lymphom-2 mRNA expression (p < 0.05). Compared with green LED light, red LED light inhibited FOXO1 mRNA expression in rabbit ovaries (p < 0.05). Red light can affect the reproductive performance of female rabbits and the expression of key genes for follicular development.