• Title/Summary/Keyword: total bacteria

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Scientific Consideration in Determining Shelf Life of Market Milk (시유의 유통기간 결정에 관한 학문적 고찰)

  • Choi, Suk-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.22 no.1
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    • pp.27-35
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    • 2004
  • The shelf lift of market milk should be determined based on the flavor which is influenced by environmental and sanitary conditions of dairy farm, milk processing plant, and storage and transportation facility as well as compositional quality, such as protein and fat, of the milk itself. The legal shelf life of market milk is often limited by microbiological quality, e.g. total bacterial count, coliform count, and food poisoning bacteria. The bacteria involved with milk spoilage and poisoning are originated from bacteria contaminating milk after pasteurization or spores surviving the heat treatment of pasteurization. The important factors which influence the shelf life of market milk are microbiological quality of raw milk, pasteurization condition, post-pasteurization contamination, and temperature during storage and transportation. The organoleptic quality and shelf life of market milk should be further improved by satisfying the consumer's taste, which depends on somatic cell count and bacterial count of milk, feed quality, foreign substance in milk, and physical treatment during processing and transportation.

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Effect of Colloids on Contaminant Transport in Riverbank Filtration (강변여과에서 콜로이드 물질이 오염물 거동에 미치는 영향)

  • Kim, Dae-Hwan;Lee, Sang-Il;Yoo, Sang-Yeon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2005.05b
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    • pp.1398-1402
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    • 2005
  • Riverbank filtration is a natural process using alluvial aquifers to remove contaminants and pathogens in river water for the production of drinking water. In riverbank filtration, the understanding of contaminant transport is an important task for the production of high quality drinking water. This study investigates the transport behavior of hydrophobic organic contaminants when colloids (dissolved organic matter and bacteria) are present in the aquifer. A mathematical model for the transport of contaminants is developed and solved numerically for various situations. Results show that in the riverbank filtration the presence of DOM and bacteria enhances the mobility of contaminant significantly. Sensitivity analysis indicates that the distribution of the total aqueous Phase contaminant is significantly affected by distribution coefficients which account for affinity of solid or colloidal Phase to contaminant.

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Distribution and Activity of Hheterotrophic Bacteria in the Mudflat of Nakdong River Estuary (난동감 하구 간석지에 존재하는 세균의 분포 및 생리적 활성도)

  • Kim, Sang-Jong;Hong, Soon-Woo;Rhie, Youn;Choi, Sung-Chan
    • Korean Journal of Microbiology
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    • v.23 no.3
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    • pp.215-222
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    • 1985
  • Distribution pattern and activity of heterotrophec bacteria were measured in the mudflat of Nakdong river estuary. In March and June, 1985, community sizes of amylolytic, lipolytic and proteolytic bacteria as well as total viable counts were measured. Vertical distribution of bacterial community size increased a few orders of magnitude from Narch to June. Heterotrophic activity was estimated in turnover time with $U-[^{14}C]-glucose$. Turnover time reduced considerably in June compared to that of March. To sxamine correlations for measured bacterial groups, turnover time and environmental factors, correlation coefficient matrix was obtained. These measured characteristics did not consistently correlate well with one another.

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Enumeration and Recovery of Bacterial Isolates from Ruminants Fed with Different Dietary Regimes and Their Antibacterial Activity

  • Pattnaik, P.;Grover, Sunita;Batish, V.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.6
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    • pp.811-815
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    • 2001
  • The study evaluated different synthetic and semisynthetic media for maximal recovery of rumen bacteria and expression of their antibacterial activity. Rumen Glucose Cellobiose Agar (RGCA) medium was found to be the best for recovery of rumen bacteria. However, L-10 medium was the best for expression of antibacterial activity of ruminal isolates followed by Easy, M-10, RGCA and M-98-5 medium. The present study recommends the use of L-10 medium as the medium of choice for screening of antibacterial activity of ruminal isolates. Comparative evaluation of bacterial counts on different dietary regimes indicated significant difference between different growth media on a specific diet and between diets on specific growth media within a species. However, there is no overall significant difference between total bacterial counts obtained from rumen liquor of cattle and buffalo with respect to either the feeding regime or growth media. Feeding straw based diet to the animal is the best for high recovery of rumen bacteria.

Numerical Taxonomic Studies of Phenol-degrading Bacteria Isolated from Sail (토양에서 분리한 Phenol 분해세균의 수치분류)

  • Lee, Geon;Lee, Sang-Joon;Lee, Jong-Kun
    • Microbiology and Biotechnology Letters
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    • v.19 no.6
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    • pp.624-630
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    • 1991
  • Sixty five phenol degrading bacteria were isolated from soil and identified. Sirnility values calculated on the basis of total 46 morphological, biochemical and physiological characteristics of the isolated strains. 65 isolates were divided into 6 clusters at the 70% simility lavei, The dominant organisms were belonged to Azotobacter, Pseudomonas and Flavobactwium.

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Isolation of Antibiotic-producing Bacteria Antagonistic to Fusarium oxysporum from Sesame-growing Soils and Evaluation of Their Antifungal Activity

  • Lee, Yong Se;Ho Young Lee;Chang Ho Lee;Hee Sung Park
    • Journal of Microbiology and Biotechnology
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    • v.5 no.6
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    • pp.346-352
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    • 1995
  • For isolation of antibiotic-producing bacteria antagonistic to Fusarium oxysporum, a total of 327 microorganisms were screened from sesame-growing soils collected at various locations in Korea by the modified Herr's triple-agar-Iayer technique. Among the 36 bacterial isolates further screened by the dual culture test on tryptic soy agar, 10 were tested to show their antagonistic activity against 14 plant pathogenic fungi. Bacterial culture filtrates were shown either to inhibit some phytopathogenic fungal growth or to suppress F. oxysporum infection of sesame plants maintained in the green house. An isolate, B23, with the most prominent antagonistic activity was identified as Bacillus subtilis.

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Effectiveness of Antimicrobial Starch Coating Containing Thyme Oil against Salmonella, Listeria, Campylobacter, and Pseudomonas on Chicken Breast Meat

  • Goswami, Namita;Han, Jung-Hoon;Holley, Richard A.
    • Food Science and Biotechnology
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    • v.18 no.2
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    • pp.425-431
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    • 2009
  • Antimicrobial coating on chicken carcasses may reduce the effects of cross-contamination and improve product shelf-life and safety. Thyme oil was mixed at 0.5%(v/v) with a pre-gelatinized pea starch coating solution. The coating solution was spread on chicken breast meat after inoculation with selected spoilage and pathogenic bacteria. After inoculation, the chicken meats were packaged in plastic bags and stored at $4^{\circ}C$. During 12 day storage, total aerobic bacteria, lactic acid bacteria, and inoculated organisms were counted at 4 day intervals. Thyme oil treatments reduced the viability of Salmonella as well as the growth of Listeria and Pseudomonas by 2 log CFU/g, and appeared to eliminate inoculated Campylobacter during storage. The addition of thyme oil increased the viscosity of the pre-gelatinized pea starch solution. The results suggested that thyme oil inclusion in an edible starch coating may be a satisfactory delivery system to enhance the safety of processed fresh meat.

Cholesterol-Lowering Lactic Acid Bacteria from Kimchi

  • Cho, Gyu-Sung;Lee, So-Yeoun;Kim, Young-Nam;Shin, Hyeun-Kil
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2005.10a
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    • pp.324-328
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    • 2005
  • Lactic acid bacteria are dominant microflora in fermented kimchi. In this study, lactic acid bacteria were isolated from 5 kinds of fermented kimchi and determined their physiologic property. Total 64 of LAB were isolated from kimch samples. In this study, 8 strains of them were selected by pH and bile salt tolerance test. HFI 58, HFI 40, and Yeulmu E strain had significant cholesterol lowering effect in vitro test. These were made of special feed of chicken by WooJin B&G. A Diet was tested for 5 weeks. The feed of special material supplement HFI58 groups had significant lower cholesterol concentration in egg yolk.

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Changes in Enzyme Activities and Population of Lactic Acid Bacteria during the Kimchi Fermentation Supplemented with Water Extract of Pine Needle (솔잎(Pinus densiflora Sieb. et Zucc.) 물추출물 첨가김치의 숙성 중 젖산균수와 효소활성의 변화)

  • 오영애;최경호;김순동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.2
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    • pp.244-251
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    • 1998
  • To understand the effect of supplement of water extract of pine needle(WEPN) on shelf-life enhancement of the kimchi, activities of four enzymes and number of lactic acid bacteria, during fermentation of the kimchi, were assayed. Enzyme activities of kimchi fermented for 7 days with supplement by 2% water extract of pine needle showed amylase of 86.4%, protease of 85.8%, polygalacturonase of 61.5% and $\beta$-galactosidase of 58.8% against the control kimchi. WEPN showed weak inhibitory effect when it was applied to the isolated enzymes in vitro then those menifested by the kimchi in vivo. Number of total bacterial cell of WEPN supplemented kimchi increased by 10 folds than control between 7 to 14 days of fermentation. On contrast, number of lactic acid bacteria decreased maximaly to 21% of control by fermentation. The clear zone formed on paper disk by WEPN against L. plantarum was larger than that of Leu. mesenteroides.

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Source Environment Feature Related Phylogenetic Distribution Pattern of Anoxygenic Photosynthetic Bacteria as Revealed by pufM Analysis

  • Zeng, Yonghui;Jiao, Nianzhi
    • Journal of Microbiology
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    • v.45 no.3
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    • pp.205-212
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    • 2007
  • Anoxygenic photosynthesis, performed primarily by anoxygenic photosynthetic bacteria (APB), has been supposed to arise on Earth more than 3 billion years ago. The long established APB are distributed in almost every corner where light can reach. However, the relationship between APB phylogeny and source environments has been largely unexplored. Here we retrieved the pufM sequences and related source information of 89 pufM containing species from the public database. Phylogenetic analysis revealed that horizontal gene transfer (HGT) most likely occurred within 11 out of a total 21 pufM subgroups, not only among species within the same class but also among species of different phyla or subphyla. A clear source environment feature related phylogenetic distribution pattern was observed, with all species from oxic habitats and those from anoxic habitats clustering into independent subgroups, respectively. HGT among ancient APB and subsequent long term evolution and adaptation to separated niches may have contributed to the coupling of environment and pufM phylogeny.