• Journal of Life Science
• /
• 제27권6호
• /
• pp.688-693
• /
• 2017

Antifungal bacterium against Colletotrichum coccodes causing black dot disease of potatoes and anthracnose of tomatoes was isolated from sewage sludge. The isolate showed a 99% sequence homology of partial 16S rRNA of Bacillus methylotrophicus CBMB205 and Bacillus amyloliquefaciens subsp. plantarum FZB42. The isolate was identified as Bacillus sp. SW29-2, using the neighbor-joining phylogenetic tree, BlastN sequence analysis, and morphological and cultural characteristics. Bacillus sp. SW29-2 is an aerobic, Gram-positive, endospore-forming bacterium, of which the morphological and physiological characteristics were the same as those of type strain B. lichniformis CBMB205, except for the cell growth of over 4% NaCl. The cell growth of the temperature and the initial pH of the medium was shown at $18-47^{\circ}C$ (opt. ca. $38^{\circ}C$) and 3-9 (opt. ca. 6.0), respectively. The inhibition size (diameter) of Bacillus sp. SW29-2 against four strains of C. coccodes ranged from 23 to 29 mm. Also, the isolate showed antifungal activity against penicillium rot-causing Penicillium expansum in apples. Thus far, any report on the antifungal activity of Baciilus spp. against C. coccodes has not been found. These results suggest that the Bacillus sp. SW29-2 isolate could be used as a possible biocontrol agent against C. coccodes, and further applied to other plant pathogenic fungi.

• Korean journal of applied entomology
• /
• 제53권4호
• /
• pp.435-440
• /
• 2014

The number of Bemisia tabaci adults attracted to horseweed, thistle, red bean, cucumber, and tomato plants, as measured by the number stuck to yellow sticky traps, was investigated in a polyvinyl greenhouse for growing tomatoes using organic methods. The number was highest on cucumber plants in the early days of B. tabaci infestation, but was highest on horseweed overall. Of the eight species of beans tested, B. tabaci adults were most attracted to Ultari-Gangnang; however, beans were not effective trap plants for B. tabaci. Measurement of olfactory behavioral response using a four-choice olfactometer showed that B. tabaci adults preferred horseweed to cucumber, eggplant, or tomato. The developmental period of B. tabaci eggs and larvae was shorter at high temperature. At $30^{\circ}C$, the developmental period of eggs and larvae on horseweed were reduced approximately 0.5 and 1 day, respectively, compared to those on cucumber or tomato.

• Korean journal of applied entomology
• /
• 제52권1호
• /
• pp.23-27
• /
• 2013

Investigations were conducted to prevent entrance of Bemisia tabaci (Gennadius) with very fine mesh nets. B. tabaci vectors tomato yellow leaf curl virus in tomatoes. B. tabaci could be completely prevented to penetrate into an 83 mesh net, though few of them passed the 60 mesh net. An independent 60 mesh net cage was built within one of the three interconnected common plastic greenhouse ($21.0{\times}18.3m$) in order to find its usefulness as a crop protection tool from insect pests. TYLCV infected tomato plants were not detected, though a few eggs of B. tabaci and mines of Liriomyza trifolii (Burgess) were detected locally at the entrance of the sliding door. There were practically no significant differences between treatments of nets and the conventional plant height, leaf length and width, number of floret clusters, sugar brix, and product yield. In viewpoints of commercialization, it could be considered highly prospectful to apply to the nets to glasshouses or other greenhouses in which the growing period of crops is long, if the nets were used in the openings of sides, ceilings, and entrances of the greenhouse. However, it might be necessary to take preventive measures for occurrences of insect pests living in soil, temporarily or permanently, as well as high humidity diseases.

• Horticultural Science & Technology
• /
• 제33권4호
• /
• pp.566-574
• /
• 2015

Yellow or transparent fruit peel color is caused by the accumulation or lack of naringenin chalcone (NG, C) in fruit peel and determines the red or pink appearance of tomato fruit, respectively. NGC biosynthesis is regulated by the SlMYB12 gene of the Y locus on chromosome 1, and DNA markers derived from SlMYB12 would be useful for marker-assisted selection (MAS) of tomato fruit color. To develop a gene-based marker, 4.9 kb of the SlMYB12 gene including a potential promoter region was sequenced from the red-fruited (YY) line 'FCR' and pink-fruited (yy) line 'FCP'. Sequence alignment of these SlMYB12 alleles revealed no sequence variations between 'FCR' and 'FCP'. To identify SlMYB12-linked single nucleotide polymorphisms (SNPs), 'FCR' and 'FCP' were genotyped using a SolCAP Tomato SNP array and CAPS markers (CAPS-456, 531, 13762, and 38123) were developed from the four SNPs (solcap_snp_sl_456, 531, 13762, and 38123) most closely flanking the SlMYB12. These CAPS markers were mapped using $F_2$ plants derived from 'FCR' ${\times}$ 'FCP'. The map positions of the fruit peel color locus (Y) were CAPS-13762 (0 cM) - 456 (11.09 cM) - Y (15.71 cM) - 38123 (17.82 cM) - 531 (30.86 cM), and the DNA sequence of SlMYB12 was physically anchored in the middle of CAPS-456 and CAPS-38123, indicating that fruit peel color in domesticated tomato is controlled by SlMYB12. A total of 64 SolCAP tomato germplasms were evaluated for their fruit peel color and SNPs located between solcap_snp_sl_456 and 38123. Seven SNPs that were detected in this interval were highly conserved for pink-fruited accessions and specific to transparent fruit peel traits, as depicted by a phenetic tree of 64 accessions based on the seven SNPs.

• The Plant Pathology Journal
• /
• 제23권3호
• /
• pp.143-150
• /
• 2007

A new virus-like disease of tomato showing chlorotic spots, malformation and necrosis on leaves, and chlorotic blotching, rings, and necrosis on fruits was observed around Sacheon, Gyeongsangnam-do, Korea in 2004. Host range analysis could not differentiate 4 field isolates collected from tomatoes showing different symptoms but identified them as Tomato bushy stunt virus (TBSV). TBSV-tsf2 isolate induced symptoms in indicator plants similar to those caused by the TBSV-C, -S and -Nf. As the isolate could not systemically infect Chenopodium quinoa, the isolate might belong to the previously described TBSV-S isolate. TBSV-tsf2 isolate caused similar cytological alterations that were similar to that generally caused by previously reported TBSV isolates. TBSV-tsf2 isolate, however, could be considered to belong to new strain of TBSV because masses of small electron-dense patches that were not observed from the previously described TBSV. The complete nucleotide sequences of the genomic RNA of 4739 nt excluding non-translated sequences at both termini have been determined and compared to sequences of other TBSV strains. The complete nucleotide sequence identity among TBSV isolates was 98.9% to 99.7%, and to the other tombusviruses ranged from 80.8% to 94.9%. Comparison of the amino acid sequences all five ORFs with those of other TBSV strains shows a similar genomic organization, and high percentage of amino acid sequence homology with TBSV-Nf than TBSV-S isolate. Since the TBSV symptoms were only observed in Sacheon fields where imported seeds from Japan were planted, the TBSV incidence probably caused by the planting contaminated tomato seeds and thus require more through quarantine procedure to prevent settlement of TBSV in Korea. Altogether, these results support that the Korean isolate of TBSV infecting tomato might be new strain.

• Research in Plant Disease
• /
• 제15권1호
• /
• pp.1-7
• /
• 2009

Viruses diagnosed on crops including rice plants from farmers or agricultural extension agencies cover the country were 11 species including Broad bean wilt virus 2 (BBWV2) in 2008. Tomato spotted wilt virus (TSWV) was the most important virus having the detection rate of 22.9%. Two viruses of Tomato yellow leaf curl virus (TYLCV) and Tobacco leaf curl virus (TLCV) inducing leaf yellow and curl diseases on tomatoes were occurred newly with the detection rate of 12.2% and 4.0%, respectively, in 2008. Rice stripe virus (RSV) was occurred on 869.5 ha mainly at Jindo and Haenam areas in Jeollanamdo province. At Jindo area, 12 plots were damaged severely with the infected hill rate of 83.8%. At the main production area of oriental melon at Seongju, almost all fruits from whole sale market at Seongju were infected with Cucumber green mottle mosaic virus (CGMMV) as the detection rate of 87%. The areas occurred TSWV in Korea were 25 totally from 2003 including 7 areas newly reported in 2008 including Naju in Jeoallanamdo. TSWV could be reduced as 0.1 % from 5.3% by covering insect proof net in vinyl house after chemical soil sterilization. Tomato yellow leaf curl disease was occurred on April in 2008 at Tongyoung area in Kyeongsangnamdo, and detected continuously at 13 areas, 7 in Kyeongsangnamdo, 4 in Jeollabukdo and 2 in Jejudo. Potato spindle tuber viroid (PSTVd) was occurred abruptly in a confined space of a civil breeding greenhouse and a cultivar evaluation field followed by disuse 17.4 M/T of potato tubers. No PSTVd was detected at 17 fields cultivated the related potatoes to the bred company by RT-PCR.

• Research in Plant Disease
• /
• 제15권1호
• /
• pp.23-29
• /
• 2009

Cooking oil and yolk mixture (COY), a environmentally acceptable plant protection agent, and COY+$CaCO_3$+neem oil mixture were studied to control the powdery mildew occurring on eggplant, paprika, cherry tomato and maturity tomato in glass houses and vinyl houses during 2005 to 2007. The morphological changes of the pathogenic fungi on the leaf surface before and after treatment of COY were observed. COY made of rape seed oil and COY+$CaCO_3$+neem oil mixture were sprayed three times with 5 days interval to foliar parts of eggplant, paprika and tomato and the disease development were examined 5 days after final spray. In eggplant, the control efficacy of COY to powdery mildew was 94.6%. In paprika, the control efficacy of COY to powdery mildew was 91.6% and that of COY+$CaCO_3$+neem oil mixture was 96.2% that revealed little higher than COY itself. In tomatoes(cherry or maturity tomato), the control efficacy of COY were about 91 %, however, when COY mixture were sprayed to tomato leaves and stems the powdery mildew was controlled completely. Typical and healthy mycelia, conidiophores and condia were observed through scanning electron microscope in COY unsprayed leaf surface, on the other hand destroyed and winkled mycelia and conidiophores were observed in COY treated leaves regardless host plants nor taxonomic differences of fungi.

• Korean Journal of Organic Agriculture
• /
• 제21권1호
• /
• pp.95-104
• /
• 2013

This research was performed to test the effects of oleic acid for the management of greenhouse whiteflies and tobacco whiteflies. Tobacco whiteflies, especially, are the vectors of tomato yellow leaf curl virus on tomato plants. Whiteflies are not only the vectors of various viruses but also the major insect pests that cause direct damages through sucking and induce sooty mold with their sweet dew on tomato plants. There are many eco-friendly management measures including the use of yellow sticky trap and natural enemies such as Eretmocerus eremicus and Amblyseius swirskii. However, these management measures have difficulties to implement in the greenhouse. Therefore, in this research, oleic acid was tested for its effect on the management of whiteflies at various concentrations of 1,000ppm, 2,000ppm, or 4,000ppm. As a result, treatments of 1,000ppm, 2,000ppm and 4,000ppm oleic acid showed the control value of 70%, 76% and 84%, respectively. In another test, treatments of 2,000ppm oleic acid, and control treatment of 1,5000ppm neem oil and 50ppm dinotefuran showed the control value of 82%, 75%, and 75%, respectively. Cost for one application of oleic acid and neem oil for 10a area would be 3,180 Won and 20,150 Won, respectively. As a result, it was assumed that the use of oleic acid would be a appropriate management measure.

• Korean Journal of Organic Agriculture
• /
• 제25권4호
• /
• pp.737-747
• /
• 2017

This experiment was carried out to determine the optimal concentration of agricultural organic materials using sulfur and oil for the insect pest control in pepper and cherry tomato cultivation. The control value of aphids and Oriental tobacco budworm (OTB) was examined one day after spraying with sulfur preparation (SP) (0.33~0.17%), oil preparations (OP) (2.00~0.33%), SP+OP, OP+ginkgo leaf extracts (GLE), SP+OP+GLE on the "Super Manidaa"pepper. The aphid control in pepper was complete by applications of SP+OP (0.25+1.00%) in the early growth stage and the control value was above 98.1% by the application of OP+GLE (1.00+1.00 %), SP+OP+GLE (0.25+1.0+1%), SP+OP+GLE (0.25+1.0+0.5%) in the middle to late growth stage while showing 0% in the control treatment. The OTB was completely controlled by the 3 times application with the high concentration of SP+OP (0.25+1.00%) in pepper cultivation. This result indicates that the oil and the sulfur preparations should be applied at low concentration before insect pests do not appeared, and then sprayed at the high concentration after they appear at pepper plant. The greenhouse whitefly in 'Minichal' tomatoes was completely controlled by three times application of SP (0.25~0.33), OP (1.0~2.00%). and all the treatment of SP+OP. However, continuous control with intervals of 1~3 days was considered favorable in the tomato plant. By the periodical control with agricultural organic materials using sulfur and oil, the greenhouse whitefly, which is a high-temperature insect pest, several moths of OTB did not occur at all. In conclusion, SP+OP (0.17%+0.33%) treatment was the most economical combination to control the aphid, OTB, and greenhouse whitefly in pepper and tomato cultivation when considering operating cost. In addition, we recommend that SP should not be sprayed on the plant shoots during the day time from July to August because of high temperature.

• Journal of Plant Biotechnology
• /
• 제47권3호
• /
• pp.218-226
• /
• 2020

This report describes methods for selecting informative single nucleotide polymorphisms (SNPs), and the development of an online Solanaceae genome database, using 234 tomato resequencing data entries deposited in the NCBI SRA database. The 126 accessions of Solanum lycopersicum, 68 accessions of Solanum lycopersicum var. cerasiforme, and 33 accessions of Solanum pimpinellifolium, which are frequently used for breeding, and some wild-species tomato accessions were included in the analysis. To select tag-SNPs, we identified 29,504,960 SNPs in 234 tomatoes and then separated the SNPs in the genic and intergenic regions according to gene annotation. All tag-SNP were selected from non-synonymous SNPs among the SNPs present in the gene region and, as a result, we obtained tag-SNP from 13,845 genes. When there were no non-synonymous SNPs in the gene, the genes were selected from synonymous SNPs. The total number of tag-SNPs selected was 27,539. To increase the usefulness of the information, a Solanaceae genome database website, TGsol (http://tgsol. seeders.co.kr/), was constructed to allow users to search for detailed information on resources, SNPs, haplotype, and tag-SNPs. The user can search the tag-SNP and flanking sequences for each gene by searching for a gene name or gene position through the genome browser. This website can be used to efficiently search for genes related to traits or to develop molecular markers.