• Korean Journal of Computational Design and Engineering
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• v.17 no.6
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• pp.398-408
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• 2012

This paper investigates the tolerance stack-up in a commercial sliding-type mobile phone model developed by a Korean electronics company, with focus on the dimensional quality of the gap between the sliding top and the main body. The tolerance analysis in this study is done using a commercial software package, which runs Monte Carlo simulations to produce the statistical distributions of the gap size at desired locations. Such an analysis revealed that the original design did not yield the desired dimensional quality of the gap. Through a series of systematic analyses and syntheses, an improved design is proposed for the nominal dimensions and tolerances of selected features of the parts. The proposed design was validated, through tolerance analysis simulation, to meet the desired requirement of the gap quality.

• Journal of the Korean Society for Precision Engineering
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• v.31 no.1
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• pp.59-66
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• 2014

The central seam, the vertical 'line' between doors, in the front view of a refrigerator must have its gap and flush within certain ranges to meet functional and aesthetic requirements. The conventional criteria for gap and flush control in the industry are to keep the gap and flush within certain ranges at each of various points along the seam. For aesthetics, however, the uniformity of the gap is also as important because a 'tapered' seam is negatively perceived by human eyes. This paper shows a case study of tolerance design for a refrigerator door system. It presents a step-by-step procedure, which consists of datum flow chain analysis, identification of assembly features, computer modeling of feature tolerances, assembly operations and measurements, tolerance simulation, and tolerance adjustments based on the simulation results. It is found that extra care may need to be used to satisfy the aesthetical criterion for gap uniformity.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.30 no.4
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• pp.363-368
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• 2012

The result of the line simplification algorithm varies with the choice of algorithms, the change in tolerance and the selection of target objects. Three of the algorithms used in this study are Sleeve-fitting, Visvalingam-Whyatt, and Bend-simplify. They were applied to the three kinds of objects which were buildings, rivers, and roads with the five degrees of the tolerance. Through this experiments the vector displacement, the areal displacement, and the angular displacement were measured and the qualitative analysis was performed with the trend line of the errors. The experimental results show that errors were differ from tolerance values, and characteristics of line simplification algorithms based on changes of tolerance were understood.

• Journal of Microbiology and Biotechnology
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• v.1 no.3
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• pp.151-156
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• 1991

In order to construct a yeast strain having high ethanol tolerance together with good lactose fermentation ability, the protoplast fusion using Saccharomyces cerevisiae STV 89 and Kluyveromyces fragilis CBS 397 was carried out. Auxotrophic mutants of K. fragilis were obtained as a selection marker by treatment of ethylmethane sulfonate. The best mutant for protoplast fusion was selected based on the capabilities of ${\beta}-galactosidase$ production and lactose fermentation. The protoplast fusion using polyethylene glycol and calcium chloride solution led to the fusion frequence of $3{\times}10^{-6}$ and a number of fusants were obtained. Among these fusants, a fusant F-3-19 showed the best results in terms of ethanol tolerance, ${\beta}-galactosidase$ activity and lactose fermentation. The performance of lactose fermentation and ethanol tolerance by this fusant were better than those of K. fragilis. Study on the ethanol tolerance having relation to fatty acid composition and intracellular ethanol concentration revealed that the fusant F-3-19 had a higher unsaturated fatty acids content and accumulated less amount of intracellular ethanol compared with a parent of K. fragilis.

• Journal of the Korea Society for Simulation
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• v.25 no.2
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• pp.83-92
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• 2016

Tolerances are defined as the allowable variations in the geometry and positioning of parts in a mechanical assembly for assuring its proper functionality. Tolerance analysis is the activity related to estimating the potential accumulated variation in assemblies. If the estimated variances go out of the specified ranges, it causes the quality problem. Thus, we should adjust the tolerances and this activity is called as tolerance design. In this paper, a case study on the accumulated tolerance analysis and design using Monte Carlo simulation is introduced, which is applied for developing a portable medical device. Using the simulation study, we can improve the assemblability and functionality of the product.

• Korean Journal of Computational Design and Engineering
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• v.11 no.2
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• pp.148-155
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• 2006

This paper presents a new method for assessing the efficiency of production process plans using tolerance chart to lower production cost. The tolerance chart is used to predict the accuracy of a part that is to be produced following the process plan, and to carry out the quantitative measurement on the efficiency of the process plan. By comparing the values of design tolerances and their corresponding resultant tolerances calculated using the tolerance chart, the process plan that is incapable of satisfying the design requirements and the faulty production operations can be identified. Similarly, the process plan that imposes unnecessarily high accuracy and wasteful production operations can also be identified. For the latter, a quantitative measure on the efficiency of the process plan is introduced. The higher the unnecessary cost of the production, the poor is the efficiency of the process plan. A coefficient is introduced for measuring the process plan efficiency. The coefficient also incorporates two weighting factors to reflect the difficulty of manufacturing operations and number of dimensional tolerances involved. To facilitate the identification of the machining operations and the machined surfaces, which are related to the unnecessarily tight resultant tolerances caused by the process plan, a rooted tree representation of the tolerance chart is introduced, and its use is demonstrated. An example is presented to illustrate the new method. This research introduces a new quantitative process plan evaluation method that may lead to the optimization of process plans.

• Journal of Environmental Science International
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• v.11 no.11
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• pp.1173-1181
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• 2002

The present study was undertaken to investigate the effect of low temperature and salicylic acid(SA) on the chilling tolerance of acclimated and nonacclimated cucumber(Cucurmis sativus L.) seedlings. The acclimation phenomenon was characterized in chilling-sensitive cucumber seedlings and found to have a significant effect on the survival and shoot dry weights. The injuries experienced by the acclimated seedlings in the third leaf stage were on average smaller by half than those experienced by the nonacclimated seedlings. Chilling also caused a large increase in the free proline levels, regardless of the acclimation status. Exogenous treatment with SA(0.5mM) resulted in improved growth and survival of the nonacclimated chilled seedlings, indicating that SA induced chilling tolerance and SA and acclimation had common effects. The application of cycloheximide in the presence of SA restored the acclimation-induced chilling tolerance. The elevated proline level observed in the cold-treated and SA-treated plants was more pronounced in the light than in the dark at a chilled temperature, indicating that endogenous proline may play a role in chilling tolerance by stabilizing the water status in response to chilling. From these results it is suggested that SA provided protection against low-temperature stress by increasing the proline accumulation, and pre-treatment with SA may induce antioxidant enzymes leading to increased chilling tolerance.

• Journal of Institute of Control, Robotics and Systems
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• v.16 no.8
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• pp.780-785
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• 2010

The OPRoS (Open Platform for Robotic Services) framework is proposed as an application runtime environment for service robot systems. For the successful deployment of the OPRoS framework, fault tolerance support is crucial on top of its basic functionalities of lifecycle, thread and connection management. In the previous work [1] on OPRoS fault tolerance supports, we presented a framework-based fault tolerance architecture. In this paper, we extend the architecture with component-based fault tolerance techniques, which can provide more simplicity and efficiency than the pure framework-based approach. This argument is especially true for fault detection, since most faults and failure can be defined when the system cannot meet the requirement of the application functions. Specifically, the paper applies two widely-used fault detection techniques to the OPRoS framework: 'bridge component' and 'process model' component techniques for fault detection. The application details and performance of the proposed techniques are demonstrated by the same application scenario in [1]. The combination of component-based techniques with the framework-based architecture would improve the reliability of robot systems using the OPRoS framework.

• Biomedical Science Letters
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• v.23 no.3
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• pp.175-184
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• 2017

Cholera toxin (CT) is an ADP-ribosylating bacterial exotoxin that has been used as an adjuvant in animal studies of oral immunization. The mechanisms of mucosal immunogenicity and adjuvanticity of CT remain to be established. In this study, we investigated the role of indoleamine 2,3-dioxygenase (IDO), which participates in the induction of immune tolerance, in CT-mediated breakdown of oral tolerance. When IDO-deficient ($IDO^{-/-}$) mice and their littermates were given oral ovalbumin, significant changes in antibody responses, footpad swelling and $CD4^+$ T cell proliferation were not observed in $IDO^{-/-}$ mice. Feeding of CT decreased IDO expression in mesenteric lymph nodes (MLN) and Peyer's patch (PP). CT-induced downregulation of IDO expression was reversed by inhibitors of nuclear factor-kappa B (NF-${\kappa}B$), pyrrolidine dithiocarbamate and p50 small interfering RNA. IDO expression was downregulated by the NF-${\kappa}B$ inducers lipopolysaccharide and tumor necrosis factor-${\alpha}$. CT dampened IDO activity and mRNA expression in dendritic cells from MLN and PP. These data indicate that CT disrupts oral tolerance by activating NF-${\kappa}B$, which in turn downregulates IDO expression. This study betters the understanding of the molecular mechanism underlying CT-mediated abrogation of oral tolerance.

• BMB Reports
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• v.46 no.1
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• pp.31-36
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• 2013

Heat shock proteins play an important role in plant stress tolerance and are mainly regulated by heat shock transcription factors (Hsfs). In this study, we generated transgenic rice over-expressing OsHsfA7 and carried out morphological observation and stress tolerance assays. Transgenic plants exhibited less, shorter lateral roots and root hair. Under salt treatment, over-expressing OsHsfA7 rice showed alleviative appearance of damage symptoms and higher survival rate, leaf electrical conductivity and malondialdehyde content of transgenic plants were lower than those of wild type plants. Meanwhile, transgenic rice seedlings restored normal growth but wild type plants could not be rescued after drought and re-watering treatment. These findings indicate that over-expression of OsHsfA7 gene can increase tolerance to salt and drought stresses in rice seedlings.