• Title/Summary/Keyword: tolerance domain

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Isolation and Identification of a New Gene Related to Salt Tolerance in Chinese Cabbage (배추에서 신규 염 저항성 관련 유전자 분리 및 검정)

  • Yu, Jae-Gyeong;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.31 no.6
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    • pp.748-755
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    • 2013
  • This study was conducted to find a salt tolerance gene in Brassica rapa. In order to meet this objective, we analyzed data from a KBGP-24K oligo chip [BrEMD (Brassica rapa EST and microarray database)] of the B. rapa ssp. pekinensis 'Chiifu' under salt stress (250 mM NaCl). From the B. rapa KBGP-24K microarray chip analysis, 202 salt-responsive unigenes were primarily selected under salt stress. Of these, a gene with unknown function but known full-length sequence was chosen to closely investigate the gene function. The selected gene was named BrSSR (B. rapa salt stress resistance). BrSSR contains a 285 bp open reading frame encoding a putative 94-amino acid protein, and a DUF581 domain. The pSL94 vector was designed to over-express BrSSR, and was used to transform tobacco plants for salt tolerance analysis. T1 transgenic tobacco plants that over-expressed BrSSR were selected by PCR and DNA blot analyses. Quantitative real-time RT PCR revealed that the expression of BrSSR in transgenic tobacco plants increased by approximately 3.8-fold. Similar results were obtained by RNA blot analysis. Phenotypic characteristics analysis showed that transgenic tobacco plants with over-expressed BrSSR were more salt-tolerant than the wild type control under 250 mM NaCl for 5 days. Based on these results, we hypothesized that the over-expression of BrSSR may be closely related to the enhancement of salt tolerance.

Design of Schroeder Method Diffusers To obtain Field Uniformity Characteristics of Reverberation Chamber (전자파 잔향실 내의 필드 균일성 확보를 위한 Shroeder 방식의 Diffuser 설계)

  • Lee Kwang-Soon;Rhee Joong-Geun;Chung Sam-Young;Kim Hye-Kwang
    • Proceedings of the Acoustical Society of Korea Conference
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    • spring
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    • pp.211-214
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    • 2000
  • 본 논문은 전자파 장해 및 복사내성 측정의 대용 시험시설로 활용될 수 있는 전자파 잔향실의 전자기장 특성에 관하여 연구하였다. 전자파 잔향실 내부 균일성을 확보하기 위하여, 음향효과의 극대화를 위해 인간의 가청 주파수대인 16-20,000Hz 범위, 음속(340 m/sec)에 적용하여 활용되고 있는 Shroeder Quadratic Residue Diffuser를 무선 주파수 대역 30 KHz -3 THz인 범위로 확장, 3가지 형태의 Diffuser를 설계하여 잔향실 내부에 적용하였다. FDTD(Finite-Difference Time-Domain) 수치해석 방식을 이용하여 잔향실 내부의 필드 특성을 조사한 결과 Diffuser를 부착한 3 가지 형태 모두 치대 tolerance $\pm$3dB 이내의 필드 균일도를 나타내었으며, Type 3, 대칭형 구조의 Diffuser 형태가 효율이 가장 우수하다는 결과를 나타내었다.

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Comparative Study of Uniform and Nonuniform Grating Couplers for Optimized Fiber Coupling to Silicon Waveguides

  • Lee, Moon Hyeok;Jo, Jae Young;Kim, Dong Wook;Kim, Yudeuk;Kim, Kyong Hon
    • Journal of the Optical Society of Korea
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    • v.20 no.2
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    • pp.291-299
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    • 2016
  • We have investigated the ultimate limits of nonuniform grating couplers (NGCs) for optimized fiber coupling to silicon waveguides, compared to uniform grating couplers (UGCs). Simple grating coupler schemes, which can be fabricated in etching steps of the conventional complementary metal-oxide semiconductor (CMOS) process on silicon-on-insulator (SOI) wafers without forming any additional overlay structure, have been simulated numerically and demonstrated experimentally. Optimum values of the grating period, fill factor, and groove number for ultimate coupling efficiency of the NGCs are determined from finite-difference time-domain (FDTD) simulation, and confirmed with experimentally demonstrated devices by comparison to those for the UGCs. Our simulated results indicate that maximum coupling efficiency of NGCs is possible when the minimum pattern size is below 50 nm, but the experimental value for the maximum coupling efficiency is limited by the attainable fabrication tolerance in a practical device process.

Bending and Pressing Tolerance of Flexible Polyoxetane based Liquid Crystalline Polymer/Low Molecular Weight Liquid Crystal Device

  • Jang, Chi-Woong;Lim, Tong-Kun;Kim, Moo-Jong;Kim, Ku-Nam;Kwon, Young-Wan;Jin, Jung-Il;Bae, Jung-Hun;Kim, Han-Sik
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07a
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    • pp.433-435
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    • 2005
  • We have studied the realigning behavior of liquid crystal molecules in liquid crystalline polymer/liquid crystal(LCP/LC) system when they are exposed to external stimulation such as bending and pressing. The birefringence of the LCP/LC in a flexible display device was measured as a function of bending or pressing deformation. The microscopic dynamic behavior of main chain, side chain, and the LC were characterized by FTIR and polarization optical microscopy. When the device is deformed in scattering memory state, liquid crystal(LC) director is found to align from randomly oriented domain state(scattering state) to homeotropic state.

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Electric Field Uniformity in Reverberation Chamber with 5 GHz Diffuser by Transmission Antenna (송신 안테나에 의한 5 GHz 이차 잔류 디퓨저를 적용한 전자파 잔향실의 내부 필드 균일도 변화)

  • Rhee, Eugene
    • Journal of the Semiconductor & Display Technology
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    • v.20 no.3
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    • pp.83-86
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    • 2021
  • In this paper, electric fields in electromagnetic reverberation chambers, which are used as a substitute facility for EM-free anechoic chambers, are analyzed. This paper focused on the 4-5 GHz band, which is expected to adversely affect equipment. To analyze the field uniformity inside the electromagnetic reverberation chamber, electric field strengths are sampled and finite-difference time-domain method was used for numerical analysis. Moreover, Quadratic residue diffuser was used to improve the characteristics of the electromagnetic reverberation chamber and the uniformity of the internal field strength. Standard deviation, tolerance characteristics, and partiality characteristics were compared while varying the aiming point of transmission antenna.

Improvement of Field Uniformity in a Reverberation Chamber with Various Numbers of a 2D CRD (2D CRD 수에 따른 전자파 잔향실 내의 필드 균일성 개선)

  • Kim, Jin-Bok;Rhee, Joong-Geun;Kim, Jung-Hoon;Rhee, Eu-Gene
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.47 no.4
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    • pp.18-24
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    • 2010
  • This paper presents the improvement of the field uniformity in a reverberation chamber which can be substitute an anechoic chamber for the electromagnetic interference (EMI) and immunity test. Nowadays, there are many EMI issues due to the increasing use of wireless local area network (LAN), digital multimedia broadcasting (DMB), and mobile internet. With this reason, this paper studied the field characteristics in a reverberation chamber for 2.3 GHz band. In this paper, the finite difference time domain (FDTD) method is used to analyze the field characteristics in a reverberation chamber. To improve the field uniformity in the reverberation chamber, this paper adopted a 2D cubical residue diffuser (CRD) with varying the disposition and number of CRD. For each case, the tolerance and standard deviation of the electric field strength are evaluated. In comparison with the reverberation chamber without any CRD, the reverberation chamber with two CRDs showed improved results; 1.98 dB improvement in standard deviation and 3.6 dB improvement in tolerance.

Expression analysis and characterization of rice oligopeptide transport gene (OsOPT10) that contributes to salt stress tolerance

  • Jung, Yu-Jin;Lee, In-Hye;Han, Kyung-Hee;Son, Cho-Yee;Cho, Yong-Gu;Lee, Myung-Chul;Kang, Kwon-Kyoo
    • Journal of Plant Biotechnology
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    • v.37 no.4
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    • pp.483-493
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    • 2010
  • Knock-out of a gene by insertional mutagenesis is a direct way to address its function through the mutant phenotype. Among ca. 15,000 gene-trapped Ds insertion lines of rice, we identified one line from selected sensitive lines in highly salt stress. We conducted gene tagging by TAIL-PCR, and DNA gel blot analysis from salt sensitive mutant. A gene encoding an oligopeptide transporter (OPT family) homologue was disrupted by the insertion of a Ds transposon into the OsOPT10 gene that was located shot arm of chromosome 8. The OsOPT10 gene (NP_001062118.) has 6 exons and encodes a protein (752 aa) containing the OPT family domain. RT-PCR analysis showed that the expression of OsOPT10 gene was rapidly and strongly induced by stresses such as high-salinity (250 mM), osmotic, drought, $100\;{\mu}M$ ABA. The subcellular localization assay indicated that OsOPT10 was localized specifically in the plasma membrane. Overexpression of OsOPT10 in Arabidopsis thaliana and rice conferred tolerance of transgenic plants to salt stress. Further we found expression levels of some stress related genes were inhibited in OsOPT10 transgenic plants. These results suggested that OsOPT10 might play crucial but differential roles in plant responses to various abiotic stresses.

Isolation and Expression of Dormancy-associated protein 1 (DRM1) in Poplar (Populus alba × P. glandulosa) (현사시나무에서 Dormancy-associated protein 1 (DRM1) 유전자의 분리와 발현특성 구명)

  • Yoon, Seo-Kyung;Bae, Eun-Kyung;Choi, Hyunmo;Choi, Young-Im;Lee, Hyoshin
    • Journal of Plant Biotechnology
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    • v.44 no.1
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    • pp.69-75
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    • 2017
  • Dormancy-associated protein (DRM) is involved in the dormancy physiology of plants and is conserved in almost all plant species. Recent studies found that DRM genes are involved in the abiotic stress response, and characterization studies of these genes have been conducted in several plants. However, few studies have focused on DRM genes in woody plants. Therefore, in this study, cDNA coding for DRM (PagDRM1) was isolated from poplar (Populus alba ${\times}$ P. glandulosa), and its structure and expression characteristics were investigated. PagDRM1 encodes a putative protein composed of 123 amino acids, and the protein contains two conserved domains (Domain I and Domain II). PagDRM1 is present as one or two copies in the poplar genome. Its expression level was highest in the stem, followed by mature leaves, roots, and flowers. During the growth of cultured cells in suspension, PagDRM1 was highly expressed from the late-exponential phase to the stationary phase. In addition, PagDRM1 expression increased in response to drought, salt stress, and treatment with plant hormones (e.g., abscisic acid and gibberellic acid). Therefore, we suggested that PagDRM1 not only plays an important role in the induction of dormancy, but also contributes to stress tolerance in plants.

Evaluation of EM Susceptibility of an PLL on Power Domain Networks of Various Printed Circuit Boards (다양한 PCB의 전원 분배 망에서의 PLL의 전자기 내성 검증)

  • Hwang, Won-Jun;Wee, Jae-Kyung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.5
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    • pp.74-82
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    • 2015
  • As the complexity of an electronic device and the reduction of its operating voltage is progressing, susceptibility test of the chip and module for internal or external noises is essential. Although the immunity compliance of the chip was served with IEC 62132-4 Direct Power Injection method as an industry standard, in fact, EM immunity of the chip is influenced by their Power Domain Network (PDN). This paper evaluates the EM noise tolerance of a PLL and compares their noise transfer characteristics to the PLL on various PCB boards. To make differences of the PDNs of PCBs, various PCBs with or without LDO and with several types of capacitors are tested. For evaluation of discrepancies between EM characteristics of an IC only and the IC on real boards, the analysis of the noise transfer characteristics according to the PDNs shows that it gives important information for the design having robust EM characteristics. DPI measurement results show that greatly improved immunity of the PLL in the low-frequency region according to using the LDO and a frequency change of the PLL according to the DPI could also check with TEM cell measurement spectrum.