• 한국가금학회지
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• 제38권2호
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• pp.129-136
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• 2011

본 실험은 ${\alpha}$-tocopherol(0, 50, 100, 200 IU/kg)과 selenium(0.3 ppm) 및 ${\alpha}$-tocopherol 100 IU/kg과 selenium 0.3 ppm을 혼합 급여한 육계의 생산성, 계육의 일반 성분, pH, TBARS, WHC, 전단력, 육색 및 지방산 조성에 미치는 영향을 알아보고자 수행하였다. 육계 360수를 공시하여 대조구와 ${\alpha}$-tocopherol 50 IU/kg 급여구를 T1, ${\alpha}$-tocopherol 100 IU/kg 급여구를 T2, ${\alpha}$-tocopherol 200 IU/kg 급여구를 T3, selenium 0.3 ppm/kg 급여구를 T4, ${\alpha}$-tocopherol 100 IU/kg과 selenium 0.3 ppm/kg 급여구를 T5 등 6개 처리구로 나누어 5주간 사양한 후 다리살 근육을 분석하였다. 육계의 증체량, 사료 섭취량 및 사료 요구율은 처리구간에 유의성이 없었다. 계육의 일반 성분중에 수분과 조단백질, 조회분은 처리구간에 유의성이 없었고, 조지방 함량은 대조구와 T1보다 처리구에서 높았다. pH, 보수성과 전단력은 모든 처리구에서 유의성은 없었다. TBARS는 대조구와 T1은 유의한 차이가 없으나, ${\alpha}$-tocopherol과 selenium 급여구에서 낮았으며, 특히 혼합급여구인 T5에서 TBARS 값이 유의하게 낮았다. 육색중 CIE $L^*$값과 $b^*$값은 처리구간에 유의성이 인정되지 않았으나, CIE $a^*$값은 T2, T3, T5에서 높았다(P<0.05). 지방산의 분석 결과, ${\alpha}$-tocopherol의 급여로 palmitic acid는 감소하고, oleic acid, linileic acid는 증가하여 포화지방산 함량은 줄고, 불포화지방산 함량이 증가하는 결과로 나타났다(P<0.05). 이상의 결과를 종합적으로 고찰해 볼때 ${\alpha}$-tocopherol과 selenium를 육계에 급여하면 육색과 지방의 산화를 억제하는 효과가 있음을 확인할 수 있어 계육의 저장성을 개선할 가능성이 있을 것으로 기대된다.

• 한국식품과학회지
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• 제35권3호
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• pp.347-352
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• 2003

본 연구는 다양한 볶음 조건에서 볶지 않은 쌀눈과 볶은 쌀눈의 품질 특성을 조사하기 위하여 관능검사, 색, tocols 함량 및 과산화물가를 측정하여 평가하였다. 관능검사와 색으로 선정된 쌀눈의 가장 기호도가 우수한 최적 볶음 조건은 $170^{\circ}C$ 20분, $180^{\circ}C$ 10분, $190^{\circ}C$ 6분이었다. 쌀눈의 Hunter a 값은 볶음 온도와 시간이 증가할수록 증가하였던 반면 L값은 볶음 과정이 진행될수록 감소하였다. 지질산화안정성에 있어서는 볶지 않은 쌀눈이 볶은 쌀눈보다 안정하였으며, $60^{\circ}C$에서 저장 9일 후 POV는 볶지 않은 쌀눈의 과산화물가는 2.0 meq/kg인데 반해 $170^{\circ}C$ 20분 처리구는 145.6 meq/kg, $180^{\circ}C$ 10분 처리구는 169.5 meq/kg, $190^{\circ}C$ 6분 처리구는 182.9 meq/kg으로 과산화물의 생성 속도가 현저하게 다름을 보여주었다. 또한 모든 쌀눈 처리구에서 4가지의 tocopherol과 3가지의 tocotrienol이 확인되었으나 ${\beta}$-tocotrienol은 검출되지 않았다. 쌀눈의 주요 tocols는 ${\alpha}$-tocopherol과 ${\alpha}$-tocotrienol이었으며 $60^{\circ}C$ 저장 중 모든 볶음 처리구에서 tocol isomer의 영향은 ${\alpha}$-tocopherol이 가장 현저한 감소를 보인 반면 ${\beta}$-tocopherol과 ${\gamma}$-tocopherol은 서서히 감소하였다. ${\delta}$-tocopherol은 tocopherol 이성체 중에 가장 높은 안정성을 보였으며 저장일별 tocotrienol 이성체들의 지질산화안정성도 tocopherol과 유사한 경향을 보였다.

• Plant Biotechnology Reports
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• 제5권1호
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• pp.19-25
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• 2011

Tocopherols belong to the plant-derived poly phenolic compounds known for antioxidant functions in plants and animals. Activation of mitogen-activated protein kinases (MAPK) is a common reaction of plant cells in defense-related signal transduction pathways. We report a novel non-antioxidant function of ${\alpha}$-tocopherol in higher plants linking the physiological role of tocopherol with stress signalling pathways. Pre-incubation of a low concentration of $50{\mu}M$ ${\alpha}$-tocopherol negatively interferes with MAPK activation in elicitor-treated tobacco BY2 suspension culture cells and wounded tobacco leaves, whereas pre-incubated BY2 cells with ${\alpha}$-tocopherol phosphate did not show the inhibitory effect on stimuli-induced MAPK activation. The decreased MAPK activity was neither due to a direct inhibitory effect of ${\alpha}$-tocopherol nor due to the induction of an inhibitory or inactivating activity directly affecting MAPK activity. The data support that the target of ${\alpha}$-tocopherol negatively regulates an upstream component of the signaling pathways that leads to stress dependent MAPK activation.

• 한국작물학회지
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• 제57권1호
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• pp.83-88
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• 2012

In order to get information on tocopherol content and composition in fifty-six soybean germplasms were evaluated by HPLC. From the principle component analysis, the first three components accounted for 71.6% of the total variance of tocopherol content in the germplasms. Principal component 1 showed significant correlations with all the morphological markers except 100-seed weight. Soybean germplasms were divided into three groups by the first two principal components. The highest content of ${\alpha}$-tocopherol was $38.6{\mu}g$ per g in Tanner, while that of ${\gamma}$-tocopherol was $195.6{\mu}g$ in PI 91073. In case of ${\delta}$-tocopherol, IT 105622 showed the highest value as $29.8{\mu}g$. The contents of tocopherol were gradually increased from the late August to late September, which was 10 days before maturity, in Alchankong and PI 96322. The tocopherol content was higher in seeds from the plants sown early than those sown late. Total tocopherol contents of PI 96188, Geomjeongkong 2, and Suwon 183 grown in Gimje were higher than those grown in Iksan, but the difference was not observed in PI 96322. These results suggest that the contents of tocopherol in soybean were affected not only by the genotypes but also by environment.

• 분석과학
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• 제15권3호
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• pp.248-255
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• 2002

The contents of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, and $\delta$-tocopherols in sesame oils were determined by high performance liquid chromatography with UV detection. ${\alpha}$-Tocopherol contents ranged from 10.28 to 19.79 mg/g oil, ${\beta}$-tocopherol contents from 8.22 to 20.10 mg/g. However, both ${\gamma}$- and $\delta$-tocopherol were less than 1.49 mg/g or not detected. ${\gamma}$-Tocopherol was not detected from both unroasted white and black sesame seed oils. Significantly higher level of tocopherol in sesame oil than other oils is an evidence of the reason why it is highly stable and prevents oxidation. The tocopherol composition for twenty sesame oils was classified by using principal component analysis.

• 한국응용과학기술학회지
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• 제6권1호
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• pp.15-19
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• 1989

The oxidative stability of palm oil with moisture addition during heationg was investigated and the antioxidant effects of tocopherol, citric acid and sodium polyphosphate were evaluated. The addition of natural tocopherol up to 200 ppm level did not improve the oxidative stability of palm oil with moisture (1m1 $H_2O$ additive to 200g of oil at $180^{\circ}C$). The maximum antioxidant effect was observed at the Concentration of 400 ppm. Addition of citirc acid did not influence the effective concentration of tocopherol. Whereas addition of sodium polyphosphate and citric acid increased the antioxidant effect of tocopherol. The maximum synergistice effect was observed at the concentrations of 50 ppm citric acid and 50 ppm of sodium polyphosphate. Addition of 50 ppm citric acid and 50 ppm sodium polyphosphate to 100 ppm of tocopherol exceeded the antioxidant effect of 400 ppm tocopherol alone.

• Archives of Pharmacal Research
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• 제13권1호
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• pp.64-68
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• 1990

The stabilization effect of .alpha.-tocopherol incorporated into liposomal phospholipid membrane was investigated by fluorospectrophotometry and UV-visible spectretarded by the presence of .alpha.-tocopherol in the bilayer of liposomal phospholipid membrane relative to cholesterol-containing liposomes and pure phospholipid liposomes. .alpha.-tocopherol-containing liposomes prolonged the oxidation of liposomes-embedded heme as those of cholesterol-containing liposomes and pure phospholipid liposomes. Thus .alpha.-tocopherol-containing liposomes may be useful for the carrier systems of nutrients and drugs to phospholipid bilayer and stabilized liposomes.

• 대한수의학회지
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• 제40권3호
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• pp.497-504
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• 2000

To document that effects of hyperbaric oxygen(HBO) and ${\alpha}-tocopherol$ on full-thickness skin grafts in rat, we performed full-thickness skin grafts bilaterally on each rats. The HBO-treated rats were received HBO twice daily for 90 minutes at 2 ATA. Surgical control rats were not treated with HBO. ${\alpha}-tocopherol$ treated rats were received the agent via oral gastric tube daily for 3 days preoperative and a fourth dose 1 to 2 hours postoperative. HBO plus ${\alpha}-tocopherol$ treated rats were received HBO and ${\alpha}-tocopherol$ as mentioned above. Biopsy specimens were taken from each rat at the time of grafting and on days 2, 4, 7, 10, 14, 21, and 28, then were processed for tissue-concentration of total glutathione(GSHt), oxidized/reduced glutathione level, and thiobarbituric acid-reactive substance(TBARS) levels. The percentage of viable graft on day 10 ranged from 67 to 93%, and was not significantly different among the each other groups. The percentage of viable graft were, however, higher in HBO plus ${\alpha}-tocopherol$ treated rats(78.6%) than in HBO alone treated rats(59.1%), ${\alpha}-tocopherol$ alone treated rats(66.7%) and surgical control rats(58.2%). TBARS concentration had a significant increase from preoperative concentration at day 2, and peak concentration at day 4(p<0.01). Concentration then decreased to preoperative concentration at day 28. GSHt concentration of free skin graft had a similar patteren of change in four groups and decreased significantly from preoperative concentration at day 2, returning to preoperative concentration by day 7(surgical control, HBO-treated, and ${\alpha}-tocopherol-treated$, alone) and 28(HBO plus ${\alpha}-tocopherol-treated$). Percentage of the concentration of reduced glutathione decreased in surgical control, HBO-treated and, ${\alpha}-tocopherol-treated$(p<0.05), and HBO plus ${\alpha}-tocopherol-treared$(p<0.01) on day 7 after surgery, whereas the concentration of oxidized increased significantly in HBO-treated(p<0.05), ${\alpha}-tocopherol-treated$(p<0.05), and HBO plus ${\alpha}-tocopherol-treated$(p<0.01).

• Reproductive and Developmental Biology
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• 제29권3호
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• pp.155-162
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• 2005

본 연구는 돼지 정자 동결시 taurine과 $\alpha$-tocopherol의 첨가가 융해후 정자 성상과 정자 기능 활성산소계(reactive oxygen species; ROS)의 발생 정도 및 지질 산화(lipid peroxidation, LPO)에 미치는 영향을 구명하기 위하여 시행하였다. 1차 및 2차 희석액내 taurine과 $\alpha$-tocopherol이 첨가된 돼지 동결정액의 융해 후 정자 운동성 양상, 정자 생존성, 정자 기법을 적용하여 다음과 같은 결과를 얻었다. Taurine(25mM, 50mM), $\alpha$-tocopherol($500{\mu}M,\;1,000{\mu}M$ 단일처리군 그리고 taurine과 $\alpha$-tocopherol의 혼합처리군($25mM\~500{\mu}M\~1,000{\mu}M$)은 동결$\cdot$융해 후 대조군과 비해 정자 운동성과 생존성은 유의적인 차이를 나타내지 않았다. Taurine과 $\alpha$-tocopherol의 혼합처리군 $50mM\~1,000{\mu}M$에서 HOST, 점체 반응이 대조군과 비교하여 유의차는 인정되지 않았으나 다소 증가하였다. 동결$\cdot$융해 정자의 ROS 발생 억제를 위한 taurine과 $\alpha$-tocopherol의 모든 처리군은 대조군과 비교하여$\cdot\{O_2}{^-}$ 발생을 유의적으로 완화시키지 못하였다. 그러나 taurine 단일처리군(25mM), $\alpha$-tocopherol 단일처리군($50mM,\;1,000{\mu}M$)과 혼합처리군($25mM\~500mM,\;50mM\~1,000{\mu}M$은 $H_{2}O_{2}$의 발생을 대조군과 비교하여 유의적으로 완화시켰다(P<0.05). 동결$\cdot$융해 정자의 malondialdehyde의 생산은 taurine과 $\alpha$-tocopherol의 모든 처리군에서 대조군과 비교하여 유의적인 감소를 보였다(P<0.05). 이상의 결과를 종합해 보면 돼지 정액의 동결보존시 taurine와 $\alpha$-tocopherol 같은 항산화제의 처리는 ROS 발생과 LPO을 효과적으로 완화시킴에 따라 돼지 정액의 동결보존 효율을 증진시킬 수 있는 유용한 방법이라 사료된다.

• 한국식품조리과학회지
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• 제27권6호
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• pp.713-721
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• 2011

Korean cabbage (Brassica campestris L.ssp.pekinensis) is one of the major cruciferous vegetables. Cruciferous vegetables contain a rare series of secondary metabolites of amino acids called glucosinolates, as well as carotenoids, tocopherol, vitamin C and fibers. This study evaluated the effect of common cooking methods (boiling, microwaving, steaming and frying) on the phytochemical content (lutein, ${\beta}$-carotene, ${\gamma}$-tocopherol, and ${\alpha}$-tocopherol), and total antioxidant capacity of Korean cabbages, determined by DPPH assay and ABTS assay. Boiling caused a decrease in carotenoids, lutein and tocopherols. Microwaving and steaming were relatively good cooking methods for maintaining lutein, ${\beta}$-carotene, ${\gamma}$-tocopherol, and ${\alpha}$-tocopherol. The overall results of this study demonstrate that some domestic cooking procedures, specifically microwave and steaming, increased the bioaccessibility of carotenoids and tocopherol, highlighting the positive role of the nutritional properties of Korean cabbage.