• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.100-105
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• 2011

This study was conducted to evaluate the effect of developed SCB(DSCB) produced by adding N, P and K to SCB liquid fertilizer on the growth of creeping bentgrass. Fertilizer treatments were designed as follows; non-fertilizer (NF), control (CF; chemical fertilizer), 100 DSCB (250 $ml{\cdot}m^{-2}$DSCB), 80DSCB (200 $ml{\cdot}m^{-2}$DSCB) and CF+SCB (CF+250 $ml{\cdot}m^{-2}$SCB). Every treatment was arranged in a randomized complete block design with three replications. In creeping bentgrass, turf color index, chlorophyll index, dry weight, shoot number and nutrient content were measured. The results were as follows; Chemical properties of soil was hardly affected by DSCB and SCB applications. Turf color index and chlorophyll index in DSCB and SCB treatment were increased by 2~3% and 14~19% than those in NF, respectively, and similar to those of CF treatment. As applied to DSCB and SCB, shoot number was increased by 7%, 21%, 36% in 100 DSCB, 80 DSCB and CF+SCB than NF, respectively, and by 19% in CF+SCB than in CF. Supplying DSCB and SCB increased dry weight of creeping bentgrass, compared to CF treatment. Compared with CF, nitrogen and P content in tissue was increased in CF+SCB and in 80DSCB, respectively. These results suggested that applications of DSCB and SCB promoted turf quality and growth of creeping bentgrass by enhancing N and P uptake and shoot number.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.39-46
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• 2015

Objective of this research was to investigate the influence of compositions and concentrations of fertilizer solutions on the vegetative growth and nutrient uptake of 'Seolhyang' strawberry. To achieve this, the solutions of acid fertilizer (AF), neutral fertilizer (NF), and basic fertilizer (BF) were prepared at concentrations of 100 or $200mg{\cdot}L^{-1}$ based on N and applied during the 100 days after transplanting. The changes in chemical properties of the soil solution were analysed every two weeks, and crop growth measurements as well as tissue analyses for mineral contents were conducted 100 days after fertilization. The growth was the highest in the treatments with BF, followed by those with NF and AF. The heaviest fresh and dry weights among treatments were 151.3 and 37.8 g, respectively, with BF $200mg{\cdot}L^{-1}$. In terms of tissue nutrient contents, the highest N, P and Na contents, of 3.08, 0.54, and 0.10%, respectively, were observed in the treatment with NF $200mg{\cdot}L^{-1}$. The highest K content was 2.83%, in the treatment with AF $200mg{\cdot}L^{-1}$, while the highest Ca and Mg were 0.98 and 0.42%, respectively, in BF $100mg{\cdot}L^{-1}$. The AF treatments had higher tissue Fe, Mn, Zn, and Cu contents compared to those of NF or BF when fertilizer concentrations were controlled to equal. During the 100 days after fertilization, the highest and lowest pH in soil solution of root media among all treatments tested were 6.67 in BF $100mg{\cdot}L^{-1}$ and 4.69 in AF $200mg{\cdot}L^{-1}$, respectively. The highest and lowest ECs were $5.132dS{\cdot}m^{-1}$ in BF $200mg{\cdot}L^{-1}$ and $1.448dS{\cdot}m^{-1}$ in BF $100mg{\cdot}L^{-1}$, respectively. For the concentrations of macronutrients in the soil solution of root media, the AF $200mg{\cdot}L^{-1}$ treatment gave the highest $NH_4$ concentrations followed by NF $200mg{\cdot}L^{-1}$ and AF $100mg{\cdot}L^{-1}$. The K concentrations in all treatments rose gradually after day 42 in all treatments. When fertilizer concentrations were controlled to equal, the highest Ca and Mg concentrations were observed in AF followed by NF and BF until day 84 in fertilization. The BF treatments produced the highest $NO_3$ concentrations, followed by NF and AF. The trends in the change of $PO_4$ concentration were similar in all treatments. The $SO_4$ concentrations were higher in treatments with AF than those with NF or BF until day 70 in fertilization. These results indicate that compositions of fertilizer solution should to be modified to contain more alkali nutrients when 'Seolhyang' strawberry is cultivated through inert media and nutri-culture systems.

• The Korean Journal of Malacology
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• v.22 no.2
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• pp.125-134
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• 2006

We investigated the reproductive cycle of the hard clam, Meretrix petechialis with its gonadal development by histological observations. The seasonal changes in biochemical component of the adductor muscle, visceral mass, foot muscle and mantle of the clam were studied by biochemical analysis, from January to December, 2002. The reproductive cycle of this species can be divided into five successive stages: early stage (January to March), late active stage (February to May), ripe stage (April to August), partially spawned stage (July to August) and spent/inactive stage (September to January). Total protein content in the visceral mass was over two times higher than that in the adductor muscle. Monthly changes of total protein content in the adductor muscle were not statistically significant (ANOVA, p = 0.071), while the changes in the visceral mass were significant (p < 0.001). Total protein content in visceral mass was higher during the early active, late active, and ripe stages (from January to May), while the lowest in July. Glycogen content in the adductor muscle was higher than that in the visceral mass. Monthly changes in glycogen contents were statistically significant in both adductor muscle (F = 237.2, p < 0.001) and the visceral mass (F = 64.04, p < 0.001). Glycogen content in the adductor muscle was the highest in the ripe stage (April). Its content was lower in the partially spawned and the spent/inactive stages (June-September). Glycogen contents in the visceral mass were relatively lower until the early active stage, while the highest in the late active stage. RNA content was higher in visceral mass than that in the adductor muscle. Monthly changes in RNA contents were significant in both adductor muscle (F = 195.2, p < 0.001) and visceral mass (F = 78.85, p < 0.001). RNA content in the adductor muscle was high in the early active stage (January-February), and then it decreased rapidly in the late active stage (March-April), thereafter, slightly increased during the partially spawned stage (June-July). RNA content in the visceral mass reached a maximum during the ripe stage (May), and then it decreased rapidly during the partially-spawned stage (June-July). There was significant positive correlation in total protein contents between adductor muscle and visceral mass (r = 0.715, p = 0.020). However, there was no correlation between adductor muscle and visceral mass in glycogen (p = 0.550), while a negative correlation was found between the adductor muscle and visceral mass in RNA (p = 0.518) contents. Especially, changes in RNA content showed a negative correlation between the adductor muscle tissue and visceral mass. Therefore, these results suggest that the nutrient content of the adductor muscle, visceral muscle and foot muscle changed in response to gonadal energy needs.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.5
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• pp.316-325
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• 2001

To develope a technique for efficiently managing fertilizer for cucumber, a quick test method to quantify nitrate content in soil solution and leaf petiole juice using a simple instrument was investigated. Among the nitrate analyzing instruments such as compact ion meter, nitrate ion meter, and test strip with reflectometer, the paper test-strip used in conjunction with a hand-held reflectometer was most closely correlated with ion chromatography method in nitrate content, and then it would be suggested with a tool that a farmer can use rapidly, conveniently and accurately for nitrate analysis in a field. Nitrate content in soil solution collected by porous cup was very variable on the lapsed time after drip irrigation and the sampling positions such as soil depth and the distance from dripper. As a result, a significant correlation between nitrate contents of soil solutions and 2M KCl soil extract was not found. However, nitrate content in soil solution extracted with a volume basis (soil:water=1:2) showed the highly significant correlation with that in 2M KCl extract. Nitrate contents of cucumber leaf petiole juices was greatly different between upper and lower leaves. Eleven to sixteen positioned-leaf would be a proper sampling position to determine nitrate content in leaf petiole for evaluating nutrient state by plant tissue analysis. From the secondary regression equations between nitrate contents of soil and petiole juice and the yield of cucumber, nitrate levels for real time diagnosis were estimated as $400mg\;l^{-1}$ soil solution by porous cup. $300mg\;l^{-1}$ in a soil volume extraction, and $1400mg\;l^{-1}$ in petiole juice from spring to summer season. In addition, the maximum yield of cucumber fruit in pot test was obtained in nitrate $1500mg\;l^{-1}$ level of petiole juice, which was similar to nitrate $1400mg\;l^{-1}$ in greenhouse trial.

• Journal of Korean Society of Forest Science
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• v.83 no.2
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• pp.164-174
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• 1994

Temporal changes in growth performances, chlorophyll contents, and tissue water relations for determining their physiological responses of five economic tree species subjected to chronic water and nutrition stresses were investigated with containerized seedlings grown in different soil moisture regimes and nutrition levels. Seedlings of Quercus acutissima, Q. variabilis, Q. mongolica, Q. serrata, and Fraxinus rhynchophylla were propagated in plastic pots(I.D. $16cm{\times}Depth$ 16cm) for the experiments. The seedlings were subjected to two soil moisture regimes of dry and wet soils and two nutrition levels of fertilization with N+P+K and no fertilization through the growing season from May to September in a green house. For the purpose of analyzing their responses to the environmental stresses, seedling heights and root collar diameters, chlorophyll contents, and P-V curve parameters of the seedlings were measured in May, July, and September. The environmental stresses coming from moisture and nutrient deficits affected the growth performances of seedlings variously among species and among different growing periods, as well as between height and basal diameter growth of seedlings. The growth performances of Q. acutissima were influenced sensitively on the stresses, but those of Q. mongolica less influenced in comparison with other species. Chlorophyll contents were generally higher in Quercus spp. than F. rhynchophylla through the growing season. The chlorophyll contents changed by species and by treatment through the season within ranges of 0.14~1.96 mg/g dry wt. of chlorophyll a and within 0.16~1.79mg/g dry wt. of chlorophyll b, respectively. But the contents seemed to be decreased gradually through the chronic environmental stresses and leaf senescence. The osmotic potential at full turgor(${\Psi}{{\pi}o}$) and turgor loss point(${\Psi}{\pi}p$) had temporarily declined up to 3 to 5bars from -7.0~-12.4bars in May to -10.2~-17.5bars in September and up to 5 to 6bars from -7.6~-14.2bars in May to -12.9~-20.4bars in September, respectively, with some exceptions. The values of ${\Psi}{\pi}p$ were generally high in F. rhynchophylla in May and July, but high in Q. serrata in September. Relative water contents at turgor loss point(RWCp) were generally high in F. rhynchophylla, but the temporal changes of RWCp were quite and frequently different among species and among treatment.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.342-351
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• 2019

The optimum N concentrations incorporated as pre-planting nutrient charge fertilizer were determined for seedling raising using cylindrical paper pots. A root medium was formulated by blending of peat moss (particles smaller than 2.84 mm were 80-90%) and perlite (1 to 3 mm) with the ratio of 7:3 (v/v). The treatment N concentrations incorporated during the root medium formulation were adjusted to 0, 150, 250, 500, and $750mg{\cdot}L^{-1}$ and the concentrations of essential nutrients except N were equal in all treatments. After making of paper pots and putting into the 40-cell tray, the seeds of Chinese cabbage ('Chunmyeong Bom Baechu') and pak-choi ('Hanog cheonggyeongchae') were sown. During the raising of seedlings, weekly analysis of medium pH, EC and concentrations of inorganic elements were conducted. After 21 and 20 days after seed sowing of Chinese cabbage and pak-choi, the growth of the above-ground parts were measured and contents of inorganic elements in the plant tissues were analyzed. During the growing period, pH of the root media rose gradually and the EC decreased rapidly at week 3. The pH of root media at harvest was in the range of 5.3 to 5.9 in Chinese cabbage and 4.93 to 5.39 in pak-choi. Growth of the aboveground parts in terms of fresh and dry weight in both the plants were the highest in the $250mg{\cdot}L^{-1}$ N treatment and the lowest in the control treatment. The elevation of pre-planting N concentrations in root medium resulted in the increase of tissue N content and decrease of P, Ca, and Mg contents. The regression equation derived from the influence of varied pre-planting N concentrations on dry weight of above-ground tissue were $y=-0.0036x^2+0.0021x+0.0635$ ($R^2=0.9826$) in Chinese cabbage and $y=-0.16x^2+0.0009x+0.032$ ($R^2=0.991$) in pak-choi. When the low critical concentration of pre-plant N is taken at the point where dry weight of above-ground tissue is 10% less than maximum (0.40 g in Chinese cabbage and 0.16 g in pak-choi), those point are 0.36 g and 0.144 g per plant in Chinese cabbage and pak-choi, respectively. The lower critical N concentrations of root media calculated from the regression equations are $196mg{\cdot}L^{-1}$ for Chinese cabbage and $187mg{\cdot}L^{-1}$ for pak-choi. These results indicate that optimum pre-plant N concentrations for seedling raising using paper pots are in the range of 196 to $250mg{\cdot}L^{-1}$ for Chinese cabbage and 187 to $250mg{\cdot}L^{-1}$ for pak-choi.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.143-148
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• 1998

This study was carried out in order to investigate oxidative deterioration during dehydration at $40^{\circ}C,\;50^{\circ}C$ and $60^{\circ}C$ of sea mussel and baby clam. Moisture content was decreased with drying temperature and time. Sea mussel was dehydrated more rapidly than baby clam that had Harder muscle tissue. Both samples were not reached to Aw 0.62 in case of 10 hrs drying at $40^{\circ}C$, But it reached within 8 hrs in sea mussel and 10 hrs in baby clam at $50^{\circ}C$, respectively. Even if $60^{\circ}C$ could speed up drying, it caused to form more free fatty acid, peroxide, thiobarbituric acid and brown pigments. Lipophilic brown pigment was 10 times higher than hydrophilic and actively increased in all samples. fluorescence intensity was also increased with drying temperature and time. Particularly, it was higher sea mussel than baby clam more or less.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.4
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• pp.579-587
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• 2020

Objective: This study evaluated the effect of yeast products on growth performance, visceral organ weights, endogenous enzyme activities, ileal nutrient digestibility and meat yield of broiler chickens fed diets containing autolyzed whole yeast (WY) and yeast cell walls (YCW) at varying levels of inclusion. Methods: Nine dietary treatments consisting of WY or YCW included at 0.5, 1.0, 1.5, or 2.0 g/kg diet and a control diet without yeast supplementation was used in the experiment. Each of the nine treatments was replicated six times with nine birds per replicate. Birds were housed in cages, in climate-controlled rooms and fed starter, grower and finisher diets. Results: There was an improvement (p<0.05) in body weight gain and feed conversion ratio on d 10, 24, and 35 for birds fed 1.0 to 2.0 g/kg WY or YCW diet. Small intestine weight was heavier on d 10 and 24 for birds on higher levels of WY and YCW compared to the control group. On d 10 and 24, there was a significant increase (p<0.05) in tissue protein content and pancreatic enzyme activities (trypsin and chymotrypsin) of birds on 1.5 to 2.0 g/kg WY and YCW diets compared to the control group. Compared to the control group, birds on WY (2.0 g/kg diet) and YCW (at 1.5 and 2.0 g/kg diet) had better (p<0.05) protein digestibility on d 24. On d 35, there was significant improvement (p<0.05) in percentage of carcass, absolute and relative breast weight for broiler chickens fed WY and YCW mostly at 2 g/kg diet compared to birds on the control diet. Conclusion: Supplementation of diets with autolyzed WY and YCW products especially at 1.5 to 2.0 g/kg diet improved broiler chicken performance and meat yield through their positive effects on ileal protein digestibility and pancreatic enzyme activities.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.260-267
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• 2016

Amino acids in the plant were intermediate metabolites which produced by uptake and assimilation of nitrogen and these extracts which gained by bio-chemical digestion from protein of plant or animal were a source of functional fertilizer. This study was conducted to evaluate effects of keratin amino acid fertilizer (KAF) gained from animal hair or hoof on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, shoot number, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), compound fertilizer (CF), keratin amino acid fertilizer treatments [CF + KAF $0.26ml\;m^{-2}$ (CKF), CF + KAF $0.52ml\;m^{-2}$ (2CKF)], and only keratin amino acid fertilizer treatment (KF). Shoot number, clipping yield, and nitrogen uptake of KF were higher than those of CF. As compared with CF, soil chemical properties, turf color index, chlorophyll index and clipping yield of keratin amino acid fertilizer were not significant, but shoot number and uptake of N and K were increased significantly. These results show that the application of keratin amino acid fertilizer increased shoot number and growth by increased uptake of nitrogen and potassium.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.12
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• pp.1681-1690
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• 2000

A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).