• Journal of Applied Biological Chemistry
• /
• v.62 no.4
• /
• pp.361-366
• /
• 2019

Isorhamnetin 3-O-glucoside, a member of the flavonol group, has been reported to be effective for inflammatory and ulcer, as well as to alleviate diabetic complications such as neuropathy, nephropathy and retinopathy. Isorhamnetin 3-O-glucoside has been extracted from several plants. Biotransformation is a valuable tool, which is used to produce value-added chemicals with inexpensive compounds. To synthesis isorhamnetin 3-O-glucoside from quercetin, two genes (PGT E82L and ROMT-9) were introduced into Escherichia coli, respectively. In order to synthesis isorhamnetin 3-O-glucoside from quercetin, a co-culture fermentation system was developed by optimizing the medium and temperature for biotransformation, the cell mix ratio, Isopropyl-β-ᴅ-thiogalactoside induction time, and quercetin feed concentration. Finally, isorhamnetin 3-O-glucoside was biosynthesized up to 181.2 mg/L under the optimized biotransformation condition, which was higher 4.7 times than previously reported (39.6 mg/L).

• Microbiology and Biotechnology Letters
• /
• v.26 no.1
• /
• pp.34-39
• /
• 1998

We have already prepared a human lysozyme (HLY) structural gene from chemically synthesized 38 oligomers with high codon usage in Saccharomyces cerevisiae. For directing the synthesis and secretion of HLY in S. cerevisiae, two types of expression vectors, a YCp centromere-based vector, pHK101 and a YEp 2-$\mu\textrm{m}$ circle-based vector, pHK501 were constructed. With the resulting plasmids, we have confirmed that yeast transformant harboring pHK501 has more secreted HLY than pHK101-transformant by using a lysoplate and a turbidimetric assay. In flask cultivation, pHK501-transformant produced active HLY about 8 times (55 units/$m\ell$) higher than pHK101-transformant. From batch cultivation, the HLY productivity was obtained with 1.12 units/$m\ell$/h, corresponding to a 1.8-fold increase compared with flask fermentation. These results indicate that yeast transformant with pHK501 vector overexpressed and secreted HLY than that of YCp type vector.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.2
• /
• pp.169-178
• /
• 2010

The present study was conducted with four ruminally fistulated cattle to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility and TDN values of various silages (whole crop rye, RS; whole crop barley, BS; Italian ryegrass, IRGS and sudangrass, SGS) which were produced in Korea. The pH of rumen fluid were not greatly affected by silage at most times after feeding but SGS decreased (p<0.049) the ruminal ammonia-N concentration as 7.28 mg/100 ml at 3h after feeding compared to other silages. An acetate proportion from SGS feeding was higher at 1h (p<0.018), 3h (p<0.004) and 6h (p<0.019) after feeding than those from other silages. The propionate ($C_2$) proportion, however, was greater (p<0.001~p<0.042) for the RS and BS than the corresponding values of other silages up to 6h after feeding while the lowest $C_2$ proportion was observed from SGS. The butyrate proportion was lowest (p<0.007~p<0.027) in the cattle fed BS at 1~6h after feeding among silages. An ED of dry matter (DM) in the rumen was highest (P<0.048) for RS as 59.64%, and was reduced in the order of BS (56.12%), IRGS (55.64%) and SGS (54.02%). Similar tendency was observed in the EDs of crude protein (p<0.014) and organic matter (OM, p<0.039). The whole tract digestibility of DM (p<0.032), neutral detergent fiber (NDF, p<0.034) and OM (p<0.041) of SGS was greatly reduced. The TDN value based on whole tract digestibility of silages was highest for RS as 61.1%, and TDN values of BS, IRGS and SGS were 57.1%, 57.9% and 50.7%, respectively. Based on the results obtained from the present study, components in various whole crop silages produced in Korea might affect the ruminal VFA proportion, and especially, NDF content could greatly influence on the TDN value of silage calculated based on the NDF content.

• KSBB Journal
• /
• v.25 no.2
• /
• pp.155-166
• /
• 2010

Studies on the production of cell-wall bound innerpolysaccharides (IPS) (soluble ${\beta}$-D-glucan) have been performed by use of suspended myelial cultures of Inonotus obliquus. This product has promising potentials as an effective antidiabetic as well as an immunostimulating agents. As a first step to enhanced production of IPS, Intensive strain improvement programs were carried out by obtaining a large amounts of protoplasts for the isolation of single cell colonies. Rapid and large screening of high-yielding producers was possible because about fivefold higher amount of protoplasts ($2.3{\times}10^6$ protoplasts/mL) could be recovered with relatively high regeneration rates of $10^{-2}{\sim}10^{-3}$ by applying a modified filtration method, as compared to the previously used trapping method. A basic protocol necessary for UV-mutation of the protoplasts was also developed, resulting in several overproducing variants with good fermentation properties. Since the amount of IPS extracted from the mycelial cell walls of I. obliquus turned out to be almost constant per g DCW, increase in cell mass was considered the most important factor for the enhancement in IPS production. Therefore, attempts were made to screen mutant cells showing rapid mycelial growth rate in the final suspended cultures. Notably, the mutant strains showing an active cellgrowth in the preceding solid growth cultures were observed to produce higher amount of IPS in the suspended fermentations as well. A striking mutant, OBLQ756-15-5 strain, obtained from the survivors of a harsh UV-treated condition (97% death rate) was found to stably produce as high cell mass as 22 g DCW/L in the final fermentations. Currently, this strain is being tested for development of a scaled-up fermentation process for mass production of IPS.

• Microbiology and Biotechnology Letters
• /
• v.41 no.3
• /
• pp.327-334
• /
• 2013

For the development of hardy kiwi wine, we arranged for the post-maturity of hardy kiwi fruit, treated them with calcium carbonate and a pectinase enzyme complex, investigated the resulting physicochemical properties and conducted a sensory evaluation. The period determined for creating post-maturity in the hardy kiwi fruit was determined as 5 days storage at room temperature following maturity. During this time the yield of fruit juice was increased from 22.1% to 53.5% using 0.1% (v/v) cytolase PCL5 for 2 h at room temperature. 0.1% (w/v) calcium carbonate was also added during the process of aging, for the reduction of the sour taste. The fermentation trial of the hardy kiwi wine was prepared using water (25% or 50%), sugar ($24^{\circ}brix$), 0.1% (w/v) $CaCO_3$, 0.1% (v/v) cytolase PCL5, $K_2S_2O_5$ (200 ppm), and yeast ($1.5{\times}10^7$ cell/ml). Fermentation then occurred for 2 weeks at $20^{\circ}C$. The pH value, total acidity, alcohol, and reducing sugar content of the resulting hardy kiwi wines of 25% (v/w) and 50% (v/w) water, were in a range of pH 3.4-3.7, 1.12-1.21%, 14.3-14.4%, and 15-16 g/l, respectively. Citric acid and fructose constituted the major organic acids and the free sugar of the 25% and 50% hardy kiwi wine, respectively. Volatile flavor components, including 10 kinds of esters, 8 kinds of alcohols, 5 kinds of acids, 3 kinds of others and aldehydes, were determined by GC analysis. The results of sensory evaluation demonstrated that 50% hardy kiwi wine is more palatable than 25% hardy kiwi wine.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.64 no.1
• /
• pp.55-62
• /
• 2019

Rapeseed meal, which is a byproduct of rapeseed oil extraction, improves crop productivity by supplying nutrients to the soil. The present study aimed to manufacture fermented rapeseed meal compost using two effective microbial agents and evaluate their efficiency as fertilizer. To types of fermented rapeseed meal, manufactured using either a bio-carrier or microbial agent, showed no differences in pH, electrical conductivity (EC), and total nitrogen content. However, the contents of $NH_4-N$ and $NO_3-N$ as inorganic nitrogen were increased by 5.6 times and 1.5 times, respectively, after 5 d of fermentation. Rapeseed meal fermented for 5 d was applied to tomato a basal fertilizer and after eight weeks, the plant height increased in all fermented rapeseed treatments compared to that in the chemical fertilizer treatment, and also the quantum yield of photosystem II (PS II) showed the same trend. The total nitrogen content of tomato leaves treated with a microbial fermented rapeseed meal was twice as high as that of that treated with a chemical fertilizer. It was confirmed that the increase in the tomato height was an effect of the rapeseed meal containing inorganic nitrogen, which can easily be absorbed by plants. From these results, it is considered that fermented rapeseed meal manufactured with an effective microbial agent for 5 d showed the highest inorganic nutrient content and greatest growth enhancement in tomato.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.12
• /
• pp.1625-1632
• /
• 2009

Four lactating Holstein Friesian crossbred cows, with an average initial weight of 450 kg, 48${\pm}$12 days in milk and initial milk yield of 18 kg/h/d, were randomly arranged according to a 2${\times}$2 factorial arrangement in a 4${\times}$4 in Latin square design with 21-d period to investigate the effects of type of total mixed ration (TMR) and type of whole cottonseed (WCS) on intake, digestibility and milk production. The dietary treatments were i) TMR and WCS supplementation at 0.5 kg/h/d, ii) TMR and cracked WCS (cWCS) supplementation at 0.5 kg/h/d, iii) fermented TMR (FTMR) and WCS supplementation at 0.5 kg/h/d, and iv) FTMR and cWCS supplementation at 0.5 kg/h/d. Voluntary feed intake was 15.9, 15.2, 15.4 and 15.6 kg DM/d in dietary treatment 1, 2, 3 and 4, respectively. Digestibility of DM, OM, CP, EE, NDF and ADF were not significantly different among dietary treatments. Ruminal pH, $NH_{3}-N$ and volatile fatty acids in the rumen were also not significantly different among type of TMR or type of WCS. Blood urea-N concentration was not significantly different among dietary treatments. Ruminal bacteria population tended to increase but ruminal protozoa population tended to decrease with supplementation of cWCS, but they were not affected by FTMR. Milk yield and 3.5% FCM were not statistically different among treatments (16.6, 16.2, 17.0, 16.3 kg/d and 18.0, 18.6, 19.9 and 19.0 kg/d, respectively). Milk composition was not significantly different among dietary treatments. However, unsaturated fatty acids in milk fat in cows fed FTMR were lower (p<0.05) than in cows fed TMR. In conclusion, fermentation is a conceivable method to improve the quality of TMR for long-time storage and the cracking method is suitable to release the fat from cottonseed for enhancing fatty acid deposition in milk. Thus, the combination of FTMR and cWCS supplementation would be an alternative strategy to improve performance of lactating cows.

• The Korean Journal of Food And Nutrition
• /
• v.17 no.1
• /
• pp.8-17
• /
• 2004

The effect of chlorella extract on the growth and acid production of yoghurt starter was investigated in order to prepare the yoghurt added with chlorella extract. The various levels of chlorella extract powder were added to skim milk medium and the medium was fermented by single or mixed culture of 4 types of lactic acid bacteria such as Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus bulgaricus. The changes in acid production(pH, titratable acidity) and number of viable cells of the medium during fermentation in skim milk added with chlorella extract powder have determined. When chlorella extract powder was added to skim milk medium at the levels of 0.5%, 1.0%, 2.0%, and 3.0%, the addition of 0.5% chlorella extract powder with the single culture of Str. thermophilus, Lac. casei, and Lac. bulgaricus showed the highest number of viable cell counts after 9 hours incubation. And also all single cultures of the yoghurt starter produced the higher amounts of acid with the addition of 0.5% chlorella extract powder. When chlorella extract powder was added to the medium at the levels of 0.25%, 0.5%, 1.0%, and 2.0%, the addition of lower lever(0.25∼0.5%) of chlorella extract powder with the mixed culture of the lactic acid bacteria showed more the acidity of pH and the number of viable cell counts. Among the treatments tested, the addition of 0.25% chlorella extract powder with the mixed culture of Str. thermophilus and Lac. casei produced the highest number of viable cell counts after 12 hours incubation. Therefore it was suggested to manufacture the yoghurt with the addition of 0.25% chlorella extract powder and the inoculation of mixed culture of Str. thermophilus and Lac. casei for on the stimulation of growth of the yoghurt starter.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.4
• /
• pp.496-500
• /
• 2001

In vitro and in vivo experiments were carried out to evaluate the nutritive value of three tropical browse species as assessed by DM and CP digestibility, and NDF and ADF degradability with incubation times (T) of 6, 12, 24 and 48h. During the in vivo digestibility experiment three male castrated sheep (age 16 - 25 months) with a mean liveweight of $11.5{\pm}0.9kg$ were placed in individual metabolism stalls and were allocated to one of the three browse species in a $3{\times}3$ Latin square design. The browse species were all leguminous and consisted of: Acacia sieberina (A. sieberina), Ficus polita (F. polita), and Ficus sycomorus (F. sycomorus). The mean DM and CP contents of F. polita were higher than for A. sieberina and F. sycomorus (p<0.05). In contrast the NDF and ADF contents of F. sycomorus were higher compared to the other species examined (p<0.05). The in vitro DM and CP digestibility, and NDF and ADF degradability observed at different stages of incubation were higher in F. polita followed by A. sieberina and F. sycomorus. The DM and CP digestibility at 48 h incubation were 72.92, 74.84 and 53.52% and 77.38, 77.68 and 63.64% for A. seiberina, F. polita and F. sycomorus, respectively. This shows that F. polita contains more soluble materials which ruminant can benefit from and hence has more feeding value. The fermentation of F. sycomorus was slower for all the nutrients evaluated due to the presence of more fibre. Similarly, higher in vivo digestibility coefficient of DM, CP, NDF, ADF and hemicellulose were observed for F. polita reflecting its higher values of CP, ether extract (EE) and hemicellulose associated with lower values of NDF and ADF. Higher DMI and daily gain were recorded in sheep during feeding of F. polita compared to the other species evaluated. The digestibility of all the nutrients examined were higher in the in vivo than in the in vitro trial except for CP and DM. Sheep showed no visual signs of toxicity throughout the study periods. These results showed A. seiberina, F. polita and F. sycomorus can sustain sheep on a maintenance diet and could as well be used as a supplementary feed to low producing animals during the tropical dry season. Further research is needed to ascertain the viability of using these browse species on a long-term basis.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.1
• /
• pp.103-109
• /
• 2011

The objective of this study was to investigate the effect of enzymolytic products of soybean meal (ESBM), as one of the protein sources in the diet, on the growth performance, nutrient digestibility and immune function of weaned piglets. Soybean meal produced by bioprocessing with fermentation and enzymolysis contains reduced anti-nutritional factors and improved protein utilization. A total of 240 weaned piglets (Duroc${\times}$Landrace${\times}$Yorkshire, $9.01{\pm}0.22\;kg$ body weight) were randomly allocated to 4 treatments with 6 pens per treatment and 10 piglets per pen. The diets were based on corn-soybean meal and ESBM partially replaced soybean meal and soybean protein isolate at the inclusion level of 5, 10 or 15% in the basal diet. Feed intake and body weight were measured weekly. On days 24 to 27, faeces of each replicate were proportionally collected to determine the nutrient digestibility. On day 28 of the experiment, one piglet from each replicate was slaughtered humanely to collect immune organs. The results showed that inclusion of ESBM increased (p<0.05) the final weight, daily feed intake and daily gain of weaned pigs compared with the control diet, and ESBM at the inclusion levels of 10 and 15% improved (p<0.05) the feed/gain compared with the control diet. There were no differences (p>0.05) in daily feed intake among the levels of ESBM, but increasing the levels of ESBM from 5 to 15% improved (p<0.05) the final weight, average daily gain of pigs and feed/gain. The inclusion of ESBM at 5 to 15% increased (p<0.05) the digestibility of crude protein (CP) by 5 to 16%, and ESBM at 15% increased (p<0.05) the digestibility of digestible energy (DE), Ca and P compared with the control diet. ESBM increased (p<0.05) the relative weights of thymus and mandibular lymph nodes by 57.7 and 29.6%, respectively. The percentages of T lymphocytes, CD4+ and CD8+ in peripheral blood of weaned piglets were also increased (p<0.05) by feeding ESBM. The results suggest that ESBM can be a better protein source in improving growth performance, nutrient digestibility and immune function of weaned piglets.