• Archives of Pharmacal Research
• /
• v.25 no.4
• /
• pp.485-492
• /
• 2002

The molecular fate of thyroglobulin (Tg) is controlled by oligomerization, a means of storing Tg at high concentrations, and deoligomerization. The oligomerization of bovine Tg are intermolecular reactions that occur through oxidative processes, such as disulfide and dityrosine formation, as well as isopeptide formation; disulfide formation is primarily responsible for Tg oligomerization. Here, the protein disulfide isomerase (PDI) and/or peroxidase-induced oligomerization of unfolded thyroglobulins, which were prepared by treating bovine Tg with heat, urea or thiol/urea, was investigated using SDS-PAGE analyses. In addition, the enzymatic oligomerization was compared with non-enzymatic oligomerization. The thermally-induced oilgomerization of Tg, dependent on glutathione redox state, was affected by the ionic strength or the presence of a surfactant. Meanwhile, PDI-catalyzed oligomerization, time and pH-dependent, was the most remarkable with unfolded/reduced Tg, which was prepared from a treatment with urea/DTT, while the thermally-unfolded Tg was less sensitive. Similarly, the oligomerization of unfolded/reduced Tg was also mediated by peroxidase. However, PDI showed no remarkable effect on the peroxidase-mediated oligomerization of either the unfolded or unfolded/reduced Tg. Additionally, the reductive deoligomerization of oligomeric Tg was exerted by PDI in an excessively reducing state. Based on these results, it is proposed that PDI catalyzes the oligomerization of Tg through the disulfide linkage and its deoligomerization in the molecular fate, and this process may require a specific molecular form of Tg, optimally unfolded/reduced, in a proper redox state.

• Geomechanics and Engineering
• /
• v.18 no.2
• /
• pp.133-140
• /
• 2019

Strain-rate and temperature have significant effects on the one-dimensional (1D) compression behavior of soils. This paper focuses on the bonding degradation effect of soil structure on the time and temperature dependent behavior of soft structured clay. The strain-rate and temperature dependency of preconsolidation pressure are investigated in double logarithm plane and a thermal viscoplastic model considering the combined effect of strain-rate and temperature is developed to describe the mechanical behavior of unstructured clay. By incorporating the bonding degradation, the model is extended that can be suitable for structured clay. The extended model is used to simulate CRS (Constant Rate of Strain) tests conducted on structural Berthierville clay with different strain-rates and temperatures. The comparisons between predicted and experimental results show that the extended model can reasonably describe the effect of bonding degradation on the stain-rate and temperature dependent behavior of soft structural clay under 1D condition. Although the model is proposed for 1D analysis, it can be a good base for developing a more general 3D model.

• The Korean Journal of Physiology and Pharmacology
• /
• v.22 no.5
• /
• pp.597-605
• /
• 2018

In this study, we demonstrated the inhibitory effect of the Class Ic antiarrhythmic agent propafenone on voltage-dependent $K^+$ (Kv) channels using freshly isolated coronary artery smooth muscle cells from rabbits. The Kv current amplitude was progressively inhibited by propafenone in a dose-dependent manner, with an apparent $IC_{50}$ value of $5.04{\pm}1.05{\mu}M$ and a Hill coefficient of $0.78{\pm}0.06$. The application of propafenone had no significant effect on the steady-state activation and inactivation curves, indicating that propafenone did not affect the voltage-sensitivity of Kv channels. The application of train pulses at frequencies of 1 or 2 Hz progressively increased the propafenone-induced inhibition of the Kv current. Furthermore, the inactivation recovery time constant was increased after the application of propafenone, suggesting that the inhibitory action of propafenone on Kv current is partially use-dependent. Pretreatment with Kv1.5, Kv2.1 or Kv7 inhibitor did not change the inhibitory effect of propafenone on the Kv current. Together, these results suggest that propafenone inhibits the vascular Kv channels in a dose- and use-dependent manner, regardless of $Na^+$ channel inhibition.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.28 no.1
• /
• pp.71-78
• /
• 1992

Zr-4 used for a cladding and an end plug of reactor component has creep deformation under operation at high temperature. Creep is regarded as the time dependent deformation of a material under constant applied stress. Although the major source of the deformation of zirconium component in water-cooled reactors is irradiation creep, the thermal creep may give a rise to significant deformation in reactor component especially at relatively high temperatures and at various constant stresses, and therefore it must be predicted accurately. Stress relaxation is the time dependent change of stress at constant strain and it is a process related intimately to creep. In this paper, the creep behavior and stress relaxation of Zr-4 is examined at the temperature of 50$0^{\circ}C$ that is 40% of the absolute melting temperature of Zr-4 under the stress below yield stress and under the various constant strains. The results obtained are summarized as follows: 1) With an increase of stress, the steady state creep rate increases and the creep rupture time decreases. 2) The steady state creep rate $\varepsilon$(%/s) for the stress $\sigma$sub(c) (kgf/mm super(2)) of Zr-4 increases outstandingly. All the empirical equations computed for Zr-4 increases outstandingly. All the empirical equations computed for Zr-4 are in accord with Norton's model equation($\varepsilon$=K$\sigma$ sub(c) super (n)). The constants of materials computed are as follows: K=3.9881$\times$10 super(-5), n=1.9608 3) The rupture time T sub(r) (hr) decreases linearly with the increase of stress on the log-log scaled graph. The empirical equations computed for Zr-4 are in accord with Bailey's model equation (T sub(r)=K sub(1)$\sigma$sub(c) super(m)). The constants of materials computed are as follows: K sub(1)=1.2875$\times$10 super(16), m=-3.467 4) It seems clear that the strain could be quantitatively dependent on the high temperature creep properties such as creep stress, rupture time, steady state creep rate and total creep rate. It is found that these relationships are linear on the log-log graph. 5) In stress relaxation test, as the critical constant strain that can be allowed to the specimen is larger, stress relaxation becomes more rapid, and as the constant strain is smaller, the stress relaxation becomes slower.

• Bulletin of the Korean Chemical Society
• /
• v.16 no.11
• /
• pp.1119-1121
• /
• 1995

The method for the Schlo"gl model with the first order transition is extended to the Scho;gl model with the second order transition for a photochemical reaction. We obtain the explicit results of the time-dependent average and the time correlation function at the unstable steady state of the model in the neighborhood of the Gaussian white noise and then discuss the effect of noise on the dynamic properties.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.24 no.6
• /
• pp.792-801
• /
• 2000

The problem of phase change from liquid to solid in the inviscid plane-stagnation flow is theoretically investigated. The solution at the initial stage of freezing is obtained by expanding it in powers of time, and the final equilibrium state is determined from the steady-state governing equations. The transient solution is dependent on the three dimensionless parameters, but the equilibrium state is determined by one parameter of (temperature ratio/conductivity ratio). The effect of the fluid flow on the growth rate of the solid in the pure conduction problem can be clearly seen from the solution of the initial stage and the final equilibrium state. The characteristics of the transient heat transfer at the surface of the solid and the liquid side of the solid-liquid interface for all the dimensionless parameters are elucidated.

• Proceedings of the KIEE Conference
• /
• 2002.07d
• /
• pp.2037-2039
• /
• 2002

This paper focuses on robust $H_{\infty}$ control for bilinear systems with time-varying parameter uncertainties via state feedback. The suitable robustly stabilizing feedback control law can be constructed in term of solution to a state variable x-dependent quadratic Riccati equation using successive approximation technique. Also, the state feedback control law robustly stabilizes the plant and guarantees a robust $H_{\infty}$ performance for the closed-loop bilinear system with parameter uncertainties and exogenous disturbance.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.12 no.1
• /
• pp.1-11
• /
• 2000

This study investigates the problem of phase change from liquid to solid in the inviscid stagnation flow. The solution of dimensionless governing equations is determined by the three dimensionless parameters of (temperature ratio/conductivity ratio), Stefan number, and diffusi-vity ratio. The solution at the initial stage of freezing is obtained by expanding it in powers of time, and the final equilibrium state is determined from the steady-state governing equations. The equilibrium state is dependent on (temperature ratio/conductivity ratio), but is independent of Stefan number and diffusivity ratio. The effect of fluid flow on the pure conduction problem can be clearly seen from the solution of the initial stage and the final equilibrium state, and the characteristics of the solidification process for all the dimensionless parameters are elucidated.

• Particle and aerosol research
• /
• v.5 no.2
• /
• pp.45-52
• /
• 2009

Black carbon, which is a by-product of combustion of fossil fuel and biomass burning, is the component that imposes the largest uncertainty on quantifying aerosol climate effect. The direct, indirect and semi-direct climate effects of black carbon depend on its state of the mixing with other water-soluble aerosol components. The process that transforms hydrophobic externally mixed black carbon particles into hygroscopic internally mixed ones is called "aging". In most climate models, simple parameterizations for the aging time scale are used instead of solving detailed dynamics equations on the aging process due to the computation cost. In this study, a new parameterization for the black carbon aging time scale due to condensation and coagulation is presented as a function of the concentration of hygroscopic atmospheric components and the black carbon particle size. It is shown that the black carbon aging time scale due to condensation of sulfuric acid vapors varies to a large extent depending on the sulfuric acid concentration and the black carbon particle size. This result indicates that the constant aging time scale values suggested in the literature cannot be directly applied to a global scale modeling. The aging time scale due to coagulation with internally mixed aerosol particles shows an even stronger dependency on particle size, which implies that the use of a particle-size-independent aging time scale may lead to a large error when the aging is dominated by coagulation.

• Korean Journal of Mathematics
• /
• v.16 no.1
• /
• pp.57-84
• /
• 2008

In this paper, a boundary control problem for a flow governed by the time-dependent two dimensional Navier-Stokes equations is considered. We derive a mathematical formulation and a relevant process for an appropriate control along the part of the boundary to minimize the drag due to the flow. After showing the existence of an optimal solution, the first order optimality conditions are derived. The strict differentiability of the state solution in regard to the control parameter shall be exposed rigorously, and the necessary conditions along with the system for the optimal solution shall be deduced in conjunction with the evaluation of the first order Gateaux derivative to the performance functional.