• YAKHAK HOEJI
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• v.48 no.1
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• pp.47-54
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• 2004

ClpP is a stress-inducible protein and proteolytic subunit of the ATP-dependent Clp protease in prokaryotes and eukaryotes. Although its physiological roles in bacterial virulence were widely studied in various organsims, including Streptococcus pneumoniae, until now the immunological effect has not been investigated. Here, we have examined the cross reactivity of S. pneumoniae ClpP antibody with other organisms's cell lysate proteins. Although the protein sequence of S. pneumoniae ClpP was highly conserved among various organisms including human, the antibody rasised by S. pneumoniae ClpP was not cross-reacted with other organism's cell lysates, which were Saccharomyces cerevisiae , human lung A549 cell, Bacillus subtilis, Pseuomonas aeruginosa, E. coli, and Salmonella typhi. It was only reacted with S. pneumoniae and Lato-bacillus thermophilus. Thus we examined the immunoprotective effect of ClpP by immunizing mice with the purified ClpP. The mean survival time of mouse was significantly increased with the ClpP immunization. These results suggest that S. pneumoniae ClpP could be used as a vaccine candidate for prevention of S. pneumoniae infection.

• The Journal of Korean Medicine
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• v.21 no.1
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• pp.29-39
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• 2000

In order to elucidate the toxic mechanism of oxygen radicals in cultured mouse spinal sensory neurons, cytotoxic effect of oxygen radicals was evaluated by M1T assay and NR assay. In addition, protective effect of Sopunghwalhyultang(SPHHT) water extract on oxidant-induced neurotoxicity was investigated on these cultures. Spinal sensory neurons derived from mice were cultured in mediums containing various concentrations of Xanthine Oxidase / Hypoxanthine(XO/HX). Cell viability was measured by MTT assay and NR assay. XO/HX-mediated oxygen radicals remarkably decreased cell viability of cultured spinal sensory neurons in a dose-and time-dependent manner. And also, SPHHT blocked XO/HX-induced neurotoxicity in these cultures. These results suggest that oxygen radicals are toxic and SPHHT are effective in blocking against the oxidant-induced neurotoxicity in cultures of spinal sensory neurons of mice.

• Journal of the Korea Institute of Building Construction
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• v.13 no.1
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• pp.20-28
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• 2013

Flexure toughness of Steel Fiber Reinforced Concrete (SFRC) shows a time-dependent characteristic due to the hydration process of the cement matrix in the SFRC system. The effect of two important factors, water/cement (w/c) ratio and fiber volume fraction, on the flexure toughness development of SFRC were investigated. Three different SFRC mixtures with hooked-end steel fibers were tested using a four-point bending testing configuration. Each mixture was tested at five different ages. The results showed that the post-peak toughness of SFRC developed at an earlier age than the first-crack toughness.

• Journal of Plant Biology
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• v.29 no.4
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• pp.255-262
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• 1986

Developmental changes of chlorophyll-protein (CP)-complex and plastid membrane proteins during the greening of rape (Brassica napus L.) cotyledons were examined in order to investigate the effect of benzymladenine (BA) on plastid development. The formation of CP-complexes was slightly promoted by BA treatment in early greening stage, at 24 h and 48 h after illumination. However, BA inhibited the development of CP-complexes at 72 h after illumination. On the profiles of plastid membrane proteins with greening time, it was found that the 24 kd protein was increased and the 56 kd protein was decreased in both water control and BA-treated cotyledons. However, the above two traits were retarded under BA treatment, respectively. From the obtained result, plastid development of rape cotyledon during greening was partially affected by interaction between light and BA dependent on its physiological age.

• Journal of Ocean Engineering and Technology
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• v.15 no.2
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• pp.6-10
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• 2001

Water waves propagate over irregular bottom bathymetry are transformed by refraction, diffraction, shoaling, reflection etc. Principal factor of wave transform is bottom bathymetry, but in case of current field, current is another important factor which effect wave transformation. The governing equation of this study is develope as wave-current equation type to investigate the effect of wave-current interaction. It starts from Berkhoff's(1972) mild slope equation and is transformed to time-dependent hyperbolic type equation by using variational principal. Finally the governing equation is shown as a parabolic type equation by splitting method. This wave-current model was applied to the kwangan beach which is located at Pusan. The numerical simulation results of this model show the characteristics of wave transformation and flow pattern around the Kwangan beach fairly well.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1998.06a
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• pp.71-74
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• 1998

In this Paper, we investigate the electrical properties of ultra-thin(70${\AA}$) nitrided(NO) and reoxidized nitrided oxide(ONO) film that ale considered to be premising candidates for replacing conventional silicon dioxide film in ULSI level integration. we studied I$\sub$g/-V$\sub$g/ characteristics to know the effect of nitridation and reoxidation on the current conduction, leakage current time-dependent dielectric breakdown(TDDB) to evaluate charge-to-breakdown(Q$\sub$bd/), and the effect of stress temperature(25, 50, 75, 100$^{\circ}C$) and compared to those with thermal gate oxide(SiO$_2$) of identical thickness. From the measurement results, we find that reoxidized nitrided oxide(ONO) film shows superior dielectric characteristics, leakage current, and breakdown-to-charge(Qbd) performance over the NO film, while maintaining a similar electric field dependence compared to NO layer. Besides, ONO film has strong resistance against variation in temperature.

• Bulletin of the Korean Chemical Society
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• v.30 no.7
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• pp.1481-1484
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• 2009

When isolated phenol or a small phenol-solvent cluster is excited to the $S_1\;state\;of\;{\pi}{\pi}^*$ character, the hydrogen atom of the hydroxyl group dissociates via a ${\pi}{\sigma}^*$ state that is repulsive along the O-H bond. We computationally investigated the substitution effects of an amino group on the excited state hydrogen transfer reaction of phenol. The time-dependent density functional theory (TDDFT) with B3LYP functional was employed to calculate the potential energy profiles of the ${\pi}{\pi}^*$ and the ${\pi}{\sigma}^*$ excited states along the O-H coordinate, together with the orbital shape at each point, as the position of the substituent was varied. It was found that the amino substitution has an effect of lowering the ${\pi}{\sigma}^*$ state and enhancing the excited state hydrogen transfer reaction.

• Advanced Composite Materials
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• v.17 no.2
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• pp.111-124
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• 2008

The effect of temperature on natural frequency and damping is investigated in two different composite materials, Kevlar 29 fiber woven and polyethylene cloth, used especially to design ballistic armor. A damping monitoring method is used experimentally to measure the frequency response curve and it is also modeled numerically using a finite element program. The natural frequencies of a material, or a system, are a function of its elastic properties, dimensions and mass. This concept is used to calculate theoretical vibration modes of the composites. The damping properties in terms of the damping factor are determined by the half-power bandwidth technique. Numerically analyzed and experimentally measured time response curves are compared. It is seen that polymer matrix composites have temperature dependent mechanical properties. This relationship is functional and they have different effects against temperature.

• Journal of Welding and Joining
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• v.17 no.2
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• pp.84-90
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• 1999

The shape of weld nuggest in arc spot welding of 304 stainless steel was found by searching thermal history of a weld joint through a three-dimensional finite element model. The problem consists of one in which the finite element mesh is growing continuously in time in order to accomodate metal transfer in arc spot welding using element rebirth technique. The analysis was performed on the basis of experimental results. The finite element program MARC, along with a few user subroutines, was employed to obtain the numerical results. Temperature-dependent thermal properties, stir effect in weld pool, effect of phase transformation, and the convective and radiative boundary conditions are included in the model. Numerically predicted shape of weld nuggest is compared with the experimentally observed shape.

• Korean Journal of Materials Research
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• v.15 no.7
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• pp.431-434
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• 2005

To investigate the dependences of the absorption spectrum and electronic structure properties on the ZnO nano-particle size, ZnO nanocrystals were synthesized by a sol-gel method. The absorption onset peak exhibits a systematic blue-shift with decreasing particle size due to the quantum confinement effect, as well as, with decreasing $Zn^{2+}$ concentration. The increase of particle size is mainly controlled by coarsening and aggregation step during the nucleation and growth of ZnO nano-particles. The onset absorption spectrum of ZnO colloids changes from 310 to 355 nm as $Zn^{2+}$ concentration increases from 0.01 to 0.1 mole. The average particle size as a function of aging- time can be determined from the absorption spectra. The freshly prepared nanocrystal size was about 2.8nm.