The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Sopunghwalhyul-tang Water Extract against Xanthine Oxidase / Hypoxanthine(XO/HX)-Induced Neurotoxicity in the Cultured Mouse Spinal Sensory Neurons

소풍활혈탕 열탕액이 XO/HX에 의해 손상된 배양 척수감각신경세포에 미치는 영향

  • 양경석 (원광대학교 한의과대학 심계내과학교실) ;
  • 신선호 (원광대학교 한의과대학 심계내과학교실)
  • Published : 2000.03.01
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Abstract

In order to elucidate the toxic mechanism of oxygen radicals in cultured mouse spinal sensory neurons, cytotoxic effect of oxygen radicals was evaluated by M1T assay and NR assay. In addition, protective effect of Sopunghwalhyultang(SPHHT) water extract on oxidant-induced neurotoxicity was investigated on these cultures. Spinal sensory neurons derived from mice were cultured in mediums containing various concentrations of Xanthine Oxidase / Hypoxanthine(XO/HX). Cell viability was measured by MTT assay and NR assay. XO/HX-mediated oxygen radicals remarkably decreased cell viability of cultured spinal sensory neurons in a dose-and time-dependent manner. And also, SPHHT blocked XO/HX-induced neurotoxicity in these cultures. These results suggest that oxygen radicals are toxic and SPHHT are effective in blocking against the oxidant-induced neurotoxicity in cultures of spinal sensory neurons of mice.

Keywords

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