• 대한한방내과학회지
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• 제21권2호
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• pp.259-266
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• 2000

Objectives : The effects of aqueous extracts of Cortex ulmi pumilae (a traditional medicine for cancer treatment in oriental medicine) on the induction of apoptotic cell death were investigated in human liver origm hepatoma cell lines, HepG2. Methods : The death of HepG2 cells was markedly induced by the addition of extracts of Cortex ulmi pumilae in a dose-dependent manner. The apoptotic characteristic ladder pattern of DNA strand break was not observed in cell death of HepG2. In addition, it was not shown nucleus chromatin condensation and fragmentation under hoechst staining. However, by the using annexin V staining assay, externalizations of phosphatidylserine in HepG2 cell which were treated with Cortex ulmi pumilae extracts were detected in the early time (at 9 hr after extract treatment). Furthermore, LDH release was not detected in this early stage. Therefore, Cortex ulmi pumilae extracts-induced cell death of HepG2 cells is mediated by apoptotic death signal processes. Result : The activity of caspase 3-like proteases remained in a basal level in HepG2 cells which treated with the extract of Cordyceps sinensis. However, it was markedly increased in HepG2 cells which treated with two extracts of Cortex ulmi pumilae (C.U.P.-C, C.U.P.-K) which were differently extracted (respectively, 2.3 and 3.3 fold). On a while, the phosphotransferase activities of JNK1 was markedly induced in HepG2 cells which were treated with two extracts of Cortex ulmi pumilae. On the contrary, the activation of transcriptional activator, activating protein1(AP-1) and NF-kB were severely decreased by these two extracts of Cortex ulmi pumilae (C.U.P.-C, C.U.P.-K). In addition, antioxidants (GSH and NAC) and intracellular $Ca2^+$ level regulator (Bapta/AM and Thapsigargin) did not affect Cortex ulmi pumilae extracts-induced apoptotic death of HepG2 cells. Conclusions : In conclusion, our results suggest that two extracts of Cortex ulmi pumilae (C.U.P.-C, C.U.P.-K) induces the apoptotic death of human liver origin hepatoma HepG2 cells via activation of caspase 3-like proteases as well as JNK1, and inhibition of transcriptional activators, AP-1 and $NK-{\kappa}B$.

• Nutrition Research and Practice
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• 제7권1호
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• pp.49-58
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• 2013

The most critical point in the assessment of adherence to dietary guidelines is the development of a practical definition for adherence, such as a dietary pattern score. The purpose of this study was to develop the Korean Diet Score (KDS) based on the Korean Food Balance Wheel and to examine the association of KDS with various lifestyle characteristics and biochemical factors. The dietary data of 5,320 subjects from the 4th Korean National Health and Nutritional Examination Survey were used for the final analysis. The food guide was composed of six food group categories; 'grain dishes', 'fish and meat dishes', 'vegetable dishes', 'fruits', 'milk' and 'oils and sugars'. Based on the recommended serving numbers for each group, the scores measuring adherence to this food guide were calculated from the dietary information from the 24-hour dietary recall questionnaire, and then its correlation with various characteristics was assessed. KDS was significantly associated with several clinical, lifestyle and socioeconomic factors as well as diagnosed disease history. The higher quintile group of KDS showed a significantly lower level in fasting blood glucose, systolic blood pressure, triglycerides, current smoking and drinking as well as higher leisure time activity, house income and education. Furthermore, the KDS quintile group of women was inversely associated with hypertension, osteoporosis and diabetes. A higher KDS quintile was characterized with a higher intake of several critical nutrients, such as Ca, Fe and vitamins as well as a desirable nutrition balance such as the ratio of macronutrients. Our results demonstrate that KDS is a beneficial tool in assessing the adherence to a healthy diet based on the Korean dietary guidelines. We suggest that KDS could be a useful indicator for evaluating the dietary balance of the Korean population.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.603-613
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• 1995

The purpose of this investigation was to evaluate on the biodegradability, biocompatibility and tissue regenerative capacity of synthetic bioabsorbable membranes in beagle dogs. For animal study, 9 adult beagle dogs were used to examination, on the surgical implantation of membranes and histological analysis. In each animal, the 3rd and 4th premolars of the both sides of the mandible were selected as test teeth. Two types of bioresorbable membranes including "Guidor membrane", "S-membranes" were used to examining for biological activity, and also Gore-tex membranes was used for positive control. Surgically created defects were made in 2 premolars of both sides of the mandible at $3{\times}4mm^2$ in size and tested membranes were implanted in the defected area. A plaque control regimen was instituted with daily tooth brushing with a 0.1% chlorhexidine digluconate during experimental periods. All the experimental animals were sacrificed after 2, 4, and 8 weeks from surgery and undecalcified slides were prepared using the "sawing and grinding" technique described by Donath and Breuner". In biodegradability, all the membranes were started their biodegradation from two weeks after implantation and gradually demolished of their frame morphology from eight weeks. However, demolition of membranes in 8 weeks after implantation was highest in Guidor membranes and followed by S-membranes. Biocompatibilityof two kinds of biodegradable membranes including Guidor and S-mambrane were shown to be well tolerated to the surrounding tissue, and were minimal accumulation of inflammatory cell infiltration around the implanted membranes to compare with Gore-tex membrane. Regeneration of defected alveolar bone was initiated from two weeks of membrane implantation and new bone formation was gradually increased from that time. However, pattern of new bone formation on the defected areas of two kinds of biodegrable membranes was almost similar and quite competitive comparing with Gore-tex membrane. These results implicate that bioresorbable membranes should be highly useful tool for guided tissue regeneration of periodontal defects.

• 생물정신의학
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• 제16권1호
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• pp.5-14
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• 2009

Objectives : Most of the mechanisms reported for antidepressant drugs are the enhancement of neurite outgrowth and neuronal survival in the rat hippocampus. Neural cell adhesion molecule 140(NCAM140) has been implicated as having a role in cell-cell adhesion, neurite outgrowth, and synaptic plasticity. In this report, we have performed to elucidate a correlation among chronic antidepressant treatments, NCAM140 expression, and activation of phosphorylated cyclicAMP responsive element binding protein(pCREB) which is a downstream molecule of NCAM140-mediated intracellular signaling pathway in the rat hippocampus. Methods : Fluoxetine(10mg/kg) was injected acutely(daily injection for 5days) or chronically(daily injection for 14days) in adult rats. RNA and protein were extracted from the rat hippocampus, respectively. Real-time RT-PCR was performed to analyze the expression pattern of NCAM140 gene and western blot analyses for the activation of the phosphorylation ratio of CREB. Results : Chronic fluoxetine treatments increased NCAM140 expression 1.3 times higher than control in rat hippocampus. pCREB immunoreactivity in the rat hippocampus with chronic fluoxetine treatment was increased 4.0 times higher than that of control. Conclusion : Chronic fluoxetine treatment increased NCAM140 expression and pCREB activity in the rat hippocampus. Our data suggest that NCAM140 and pCREB may play a role in the clinical efficacy of antidepressants promoting the neurite outgrowth and neuronal survival.

• Asian-Australasian Journal of Animal Sciences
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• 제19권3호
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• pp.376-380
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• 2006

An experiment was conducted to study the effect of temperature and pH on in vitro nutrient degradability, volatile fatty acid profile and methane production. The fermenter used was the semi-continuous system, known as the rumen simulation technique (RUSITEC). Sixteen cylinders were used at one time with a volume of 800 ml, the dilution rate was set at 3.5%/hour, the infused buffer being McDougall's artificial saliva. Basal diet (9.6 g DM) used in RUSITEC consisted of (DM) 6.40 g Timothy hay, 1.86 g crushed corn and 1.34 g soybean meal. The food for the fermentation vessel was provided in nylon bags, which were gently agitated in the liquid phase. The experiment lasted for 17 d with all the samples taken during the last 5 d. Treatments were allocated at random to four vessels each and were (1) two temperature levels of $39^{\circ}C$ and $41^{\circ}C$ (2) two pH levels of 6.0 and 7.0. The total diet contained ($g\;kg^{-1}$ DM) 957 OM, 115 CP and $167MJ\;kg^{-1}$ (DM) GE. Although increase in temperature from $39^{\circ}C$ to $41^{\circ}C$ reduced degradation of major nutrients in vitro, it was non-significant. Interaction effect of temperature with pH also reflected a similar trend. However, pH showed a significant (p<0.05) negative effect on the degradability of all the nutrients in vitro. Altering the in vitro pH from 7 to 6 caused marked reduction in DMD from 60.2 to 41.8, CPD from 76.3 to 55.3 and GED from 55.3 to 35.1, respectively. Low pH (6) depressed total VFA production (61.9 vs. 34.9 mM) as well as acetate to propionate ratio in vitro (from 2.0 to 1.5) when compared to pH 7. Compared to pH 7, total gas production decreased from 1,841 ml to 1,148 ml at pH 6, $CO_2$ and $CH_4$ production also reduced from 639 to 260 ml and 138 to 45 ml, respectively. This study supported the premise that pH is one of the principal factors affecting the microbial production of volatile fatty acids and gas. Regulating the ruminal pH to increase bacterial activity may be one of the methods to optimize VFA production, reduce methane and, possibly, improve animal performance.

• 한국산학기술학회논문지
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• 제17권1호
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• pp.664-675
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• 2016

본 연구의 목적은 간호사의 시간선택제 근무에 대한 인식을 조사하고 활성화를 위한 합리적인 근무방안을 제시하는 것이다. 자료는 무작위 할당표출법을 이용하여 전국의 532명의 간호사를 대상으로 2015년 2월 10일부터 24일 까지 설문조사를 하였다. 자료 분석을 위해 SPSS 21.0 프로그램을 사용하였다. 본 연구의 결과에서 시간선택제 근무 중 단축시간제에 대한 필요성($M=3.89{\pm}0.87$)과 지원 의향(46.2%)이 가장 높게 나타났으며, 야간전담제, 휴일전담제, 2교대제 순이었다. 지원 선택의 이유로는 교대근무의 힘든 업무와 육아 병행의 어려움이 가장 높게 나타났다. 시간선택제 근무시행에 따른 긍정적인 영향으로는 자기계발 활동이나 여가활동의 증가로 삶의 질을 높일 수 있고, 육아와 일-가정 양립이 가능한 점이었으며, 부정적인 영향으로는 비정규직으로 채용될 우려와 급여가 감소할 것이라는 의견이 가장 높게 나타났다. 본 연구의 결과를 토대로 시간선택제 근무를 시행하기 위한 선결조건으로 시간선택제 근무 유형에 따른 보상과 근무조건에 대한 표준화된 운영 지침을 제시함으로써 추후에 시간선택제 간호사의 균등한 근무조건을 마련하는데 기여할 수 있다.

• The Korean Journal of Physiology
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• 제22권1호
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• pp.13-29
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• 1988

In order to elucidate the regulatory mechanism of intracellular calcium ion concentrations, contractions or contractures induced by $Na^{+}-removal$, calcium-application or ouabain-treatment as an index of $Na^+/Ca^{2+}$ exchange activity were studied in atrial muscle or vascular smooth muscle (aorta and renal artery) of the rabbit. The magnitude of low sodium contractures in atrial trabeculae increased with sigmoid shape when external sodium concentrations were reduced to sodium-free condition, whereas that of calcium contracture intensified in a parabolic pattern when external calcium concentrations were elevated to 8 mM. $Na^{+}-removal$ contractures were induced in a duration-dependent manner to $K^{+}-free$ exposure and same findings were observed with ouabain treatment. $Na^{+}-free$ contractures were not affected by verapamil treatment, but stimulated by $100{\mu}M\;Mn^{2+}$ and inhibited by high concentrations of $Mn^{2+}\;(2{\sim}8mM)$ in a dose-dependent manner. Ryanodine which is known to suppress the release of calcium from internal store abolished spontaneous twitch contractions induced by $K^{+}-free$ solution, but had no effect on the development $Na^{+}-free$ contractures. Na-free contractures were not always induced in vascular smooth muscle preparations. Contractures by $O\;mM\;Na^+$ were usually seen in aorta, but not often in renal artery.$50\;mM\;K^+$, noradrenaline (NA) and angiotensin II (AII) always evoked very large contraction in all preparations of vascular smooth muscle. Contractures developed by $O\;mM\;Na^+$ were not sensitive to verapamil treatment as in atrial trabeculae, but were abolished by $100{\mu}M\;Mn^{2+}$. In contrast to $Na^{+}-free$ contractures, $Mn^{2+}(100{\mu}M)$ had no effect on the contractures induced by NA or 50 mM$K^+$. Caffeine in the concentration of 10 mM evoked transient contracture in the distal renal artery. The rate of spontaneous relaxation in caffeine contracture was dependent upon the concentrations of external sodium, and had double component of relaxation when the rate of relaxation was plotted in the semilogarithmic scale of relative tension versus time. Especially late components of relaxation had more direct relation to $Na^+$ concentrations. It could be concluded that $Na^+/Ca^{2+}$ exchange mechanism in the heart has a large capacity, inhibited by $Mn^{2+}$ but not by verapamil and ryanodine, while $Na^+/Ca^{2+}$ exchange system in vascular smooth muscle has a very low capacity especially in small artery, inhibited by low concentration of $Mn^{2+}\;(100{\mu}M)$ but not affected by verapamil and ryanodine.

• 한국산학기술학회논문지
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• 제22권1호
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• pp.575-584
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• 2021

본 연구는 연속행동모니터링시스템(CBMS)을 사용하여 저온 충격에 노출된 참돔 Pagrus major 치어의 행동반응에 대한 임계 수온 및 행동 내성 반응을 규명하였다. 실험생물의 행동은 8.0~20.0℃ 수온구간에서 12시간과 24시간 마다 각각 2.0℃와 4.0℃씩 하강시키며 관찰하였다. 참돔 치어는 12.0℃ 이하로 수온이 하강한 경우 수온 노출시간과 변동 폭에 관계없이 유영활동이 감소하고 정지가 반복되는 불안정한 행동패턴이 관찰되었다. 또한 8.0~10.0℃ 수온에 노출된 생물의 유영능력은 급격히 저하되어 수조 바닥에서 머무르는 아치사 반응 행동을 보였다. 실험어류는 저 수온 스트레스 영향을 받아 생물의 50 %만 생존하였으며, 저온 충격(cold shock) 후 6시간 이내에 모든 개체가 사망하였다. 또한 행동지수(BI)는 급격히 감소하였으며 변이계수(CV)의 진폭변화는 타 수온보다 변동 폭이 큰 것으로 분석되었다(p<0.05). 참돔 치어의 저온 스트레스는 12.0℃를 경계로 촉진되며, 8.0~10.0℃의 저온 충격에 노출된 생물의 아치사 반응을 유발시키는 내성한계인 것으로 해석된다.

• 한국전자통신학회논문지
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• 제17권3호
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• pp.515-520
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• 2022

다중 센서를 활용하는 상황인식에 있어서 각각의 센서가 감지하여 보내온 센서 데이터를 활용할 때, 센서 별로 가중치를 달리하여야 할 필요가 있다. 같은 상황에 대하여 같은 종류의 센서를 구성하였더라도 다른 부차적인 요인 때문에 가중치 부여를 달리하여야 하는 경우가 있다. 실제 세계의 이벤트에 가중치 부여를 하지 않을 수 없으며, 다중 센서를 활용하는 상황인식 시스템에서 활용할 수 있는 가중치 부여 방안은 필요하다고 할 수 있다. 본 연구에서는 시간이 경과하면서 센서들이 계속 감지 활동을 하는 가운데 호스트로 보고하는 각 센서에 대한 가중치 부여 방안을 제안한다. 대부분의 사물인터넷 환경에서 센서는 감지 활동을 지속적으로 이어나가며, 감지한 값이 사전에 정해 둔 범위 이상의 변화양상을 보일 때, 호스트로 보고하는 것을 기본으로 한다. 이러한 것을 일종의 데이터 스트림 환경이라고 할 수 있다. 데이터 스트림 환경에서 다중 센서로부터의 감지 데이터를 대상으로 하는 가중치 부여 방안에 대하여 제안하였으며, 새로운 가중치 부여 방안은 스트림 상에서 상황 변화를 주도적으로 나타내는 데이터를 선별하여 가중치를 부여하는 것으로 하였다.

• 환경생물
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• 제42권2호
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• pp.193-206
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• 2024

본 연구는 한반도 전체 산림면적과 탄소저장량의 변화를 추정하기 위해 수행되었다. 위성영상을 활용하여 2000~2019년 기간 산림면적의 변화를 연도별로 분석하였으며, 한국형 산림탄소모형을 기반으로 2000~2020년의 산림탄소 변화를 추정하였다. 모형의 검증을 위해 국가산림자원조사 자료와 임업통계연보, European Space Agency (ESA)에서 구축한 산림 바이오매스 지도를 활용하였다. Landsat 위성자료를 기반으로 한 한반도 산림손실 면적은 478,334 ha로 추정되었으며, 북한과 남한은 각각 48.6% (232,610 ha)와 51.3% (245,725 ha)의 총 손실 면적을 차지하여 지난 20년간 북한과 남한이 비슷한 면적의 산림이 손실된 것이 확인되었다. 모델 분석 결과 2000년의 우리나라와 북한 산림의 지상부 탄소저장량은 211.5, 277.1 Tg C으로 추정되었으며, 2020년에는 각각 357.9, 417.4 Tg C으로 증가할 것으로 나타났다. 같은 기간 우리 나라와 북한 산림의 단위면적당 평균 탄소저장량은 각각 34.8, 29.4 Mg C ha^-1에서 58.9, 44.2 Mg C ha^-1으로 증가할 것으로 분석되었으며, 이러한 결과는 우리나라 산림이 북한의 산림보다 전반적인 생산성과 탄소흡수량이 높다는 것을 의미한다. 또한, ESA의 분석 결과에서 우리나라의 산림 바이오매스가 다소 낮게 추정된 것이 확인되었으며, 따라서 앞으로 보다 활발한 연구와 정보공유 등을 통해 국제사회 및 학문의 영역에서 우리나라 산림의 평가가 제고될 필요성이 있다고 사료된다. 본 연구 결과는 한반도 전체 산림 탄소 및 자원의 추정에 행정구역 및 국가 단위의 유용한 정보를 제공할 뿐만 아니라, 향후 북한 산림환경복구 계획수립을 위한 기초자료로 활용될 수 있을 것으로 기대된다.